Article ; Online: Translation of RNA from Eggs During Post-Diapause Development of Bombyx mori: (translation/RNA/diapause (egg)/Bombyx mori).
Development, growth & differentiation
2023 Volume 27, Issue 1, Page(s) 13–20
Abstract: Eggs of Bombyx mori are aroused from diapause by long-term chilling and develop when transferred to 25°C. During the first 20 hr of post-diapause development, the polysome content and the presumed rate of protein synthesis increase about 3-fold, while ... ...
Abstract | Eggs of Bombyx mori are aroused from diapause by long-term chilling and develop when transferred to 25°C. During the first 20 hr of post-diapause development, the polysome content and the presumed rate of protein synthesis increase about 3-fold, while the ribosome content and the total RNA content increase only 1.1-fold. In this study, total RNAs were extracted from chilled eggs (termed 0 hr of development), and post-diapause eggs at 10 and 20 hr of development. The RNAs were purified further by high pressure liquid chromatography to remove RNA-like oligonucleotides. On translation in a protein-synthesizing system derived from wheat germ with a subsaturating amount of RNA, no difference was found in the relative amounts of translatable mRNA activity at 10 and 20 hr of development from that at 0 hr. Moreover, the translation products of the different RNA preparations in a rabbit reticulocyte lysate system appeared very similar when separated by gel electrophoresis and located by fluorography. These facts suggest that protein synthesis in early post-diapause development is controlled at a translational level. |
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Language | English |
Publishing date | 2023-06-06 |
Publishing country | Japan |
Document type | Journal Article |
ZDB-ID | 280433-5 |
ISSN | 1440-169X ; 0012-1592 |
ISSN (online) | 1440-169X |
ISSN | 0012-1592 |
DOI | 10.1111/j.1440-169X.1985.00013.x |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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