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  1. Article ; Online: Translation of RNA from Eggs During Post-Diapause Development of Bombyx mori: (translation/RNA/diapause (egg)/Bombyx mori).

    Saito, Akira / Sugimoto, Yasushi / Koga, Katsumi / Sakaguchi, Bungo

    Development, growth & differentiation

    2023  Volume 27, Issue 1, Page(s) 13–20

    Abstract: Eggs of Bombyx mori are aroused from diapause by long-term chilling and develop when transferred to 25°C. During the first 20 hr of post-diapause development, the polysome content and the presumed rate of protein synthesis increase about 3-fold, while ... ...

    Abstract Eggs of Bombyx mori are aroused from diapause by long-term chilling and develop when transferred to 25°C. During the first 20 hr of post-diapause development, the polysome content and the presumed rate of protein synthesis increase about 3-fold, while the ribosome content and the total RNA content increase only 1.1-fold. In this study, total RNAs were extracted from chilled eggs (termed 0 hr of development), and post-diapause eggs at 10 and 20 hr of development. The RNAs were purified further by high pressure liquid chromatography to remove RNA-like oligonucleotides. On translation in a protein-synthesizing system derived from wheat germ with a subsaturating amount of RNA, no difference was found in the relative amounts of translatable mRNA activity at 10 and 20 hr of development from that at 0 hr. Moreover, the translation products of the different RNA preparations in a rabbit reticulocyte lysate system appeared very similar when separated by gel electrophoresis and located by fluorography. These facts suggest that protein synthesis in early post-diapause development is controlled at a translational level.
    Language English
    Publishing date 2023-06-06
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 280433-5
    ISSN 1440-169X ; 0012-1592
    ISSN (online) 1440-169X
    ISSN 0012-1592
    DOI 10.1111/j.1440-169X.1985.00013.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Homoeotic Gene E

    Shinohara, Yukie / Koga, Katsumi / Sakaguchi, Bungo

    Development, growth & differentiation

    2023  Volume 25, Issue 1, Page(s) 105–112

    Abstract: The formation of segments of Bombyx larvae involves differentiation of legged and legless segments during embryogenesis. Observations by light microscopy of serial sections of developing embryos of Bombyx showed that the cell number of the ectodermal ... ...

    Abstract The formation of segments of Bombyx larvae involves differentiation of legged and legless segments during embryogenesis. Observations by light microscopy of serial sections of developing embryos of Bombyx showed that the cell number of the ectodermal layers increased more rapidly in segments where legs were being extruded than in those where no appendages were formed. In the embryos of a homoeotic mutant for the E-pseudoallelic locus (about 0.0-VI), E
    Language English
    Publishing date 2023-06-06
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 280433-5
    ISSN 1440-169X ; 0012-1592
    ISSN (online) 1440-169X
    ISSN 0012-1592
    DOI 10.1111/j.1440-169X.1983.00105.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: INSTABILITY OF MESSENGER AND RIBOSOMAL RNA IN A GLUE-PROTEIN MUTANT OF Bombyx mori.

    Kamijo, Seiji / Kawaguchi, Yutaka / Fujii, Hiroshi / Sakaguchi, Bungo / Doira, Hiroshi / Kuhara, Satoru / Aso, Yoichi / Koga, Katsumi / Hayashi, Katsuya / Shimotohno, Kunitada

    Development, growth & differentiation

    2023  Volume 20, Issue 4, Page(s) 283–289

    Abstract: The mucous glands of Bombyx pupae secrete glue proteins which attach deposited eggs to the mounting sheet. A mutant of a dominant gene, named no glue (Ng), produces nonadhesive eggs which have a low capacity for glue-protein synthesis. In the present ... ...

    Abstract The mucous glands of Bombyx pupae secrete glue proteins which attach deposited eggs to the mounting sheet. A mutant of a dominant gene, named no glue (Ng), produces nonadhesive eggs which have a low capacity for glue-protein synthesis. In the present study it was shown that the mucous glands of Ng silkworms showed rapid degradation of mRNA as well as rRNA during development; this may cause the low capacity for glue-protein synthesis in the mutant organ. In contrast, the mucous glands of normal silkworms showed a significant increase in content of RNA's until the maximum rate of glue-protein synthesis was achieved. The degradation of RNA in the Ng mucous gland was inhibited by actinomycin D injected into the body fluid. Thus it is supposed that the Ng gene codes for a presumptive controller RNA, which would be the mediator of RNA instability in the mucous glands of Ng pupae.
    Language English
    Publishing date 2023-06-06
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 280433-5
    ISSN 1440-169X ; 0012-1592
    ISSN (online) 1440-169X
    ISSN 0012-1592
    DOI 10.1111/j.1440-169X.1978.00283.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

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