LIVIVO - Search results -

Search results

Result 1 - 10 of total 39

Search options

Article ; Online: Deciphering the antigen specificities of antibodies by clustering their complementarity determining region sequences.

Saputri, Dianita S / Ismanto, Hendra S / Nugraha, Dendi K / Xu, Zichang / Horiguchi, Yasuhiko / Sakakibara, Shuhei / Standley, Daron M

mSystems

2023 Volume 8, Issue 6, Page(s) e0072223

Abstract: Importance: Determining antigen and epitope specificity is an essential step in the discovery of therapeutic antibodies as well as in the analysis adaptive immune responses to disease or vaccination. Despite extensive efforts, deciphering antigen ... ...

Abstract	Importance: Determining antigen and epitope specificity is an essential step in the discovery of therapeutic antibodies as well as in the analysis adaptive immune responses to disease or vaccination. Despite extensive efforts, deciphering antigen specificity solely from BCR amino acid sequence remains a challenging task, requiring a combination of experimental and computational approaches. Here, we describe and experimentally validate a simple and straightforward approach for grouping antibodies that share antigen and epitope specificities based on their CDR sequence similarity. This approach allows us to identify the specificities of a large number of antibodies whose antigen targets are unknown, using a small fraction of antibodies with well-annotated binding specificities.
MeSH term(s)	Complementarity Determining Regions/genetics ; Antibodies/chemistry ; Antigens/chemistry ; Epitopes/chemistry ; Immunity ; Cluster Analysis
Chemical Substances	Complementarity Determining Regions ; Antibodies ; Antigens ; Epitopes
Language	English
Publishing date	2023-11-17
Publishing country	United States
Document type	Journal Article
ISSN	2379-5077
ISSN (online)	2379-5077
DOI	10.1128/msystems.00722-23
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Corrigendum to: Multiple tolerance checkpoints restrain affinity maturation of B cells expressing the germline precursor of a lupus patient-derived anti-dsDNA antibody in knock-in mice.

El Hussien, Marwa Ali / Tsai, Chao-Yuan / Satouh, Yuhkoh / Motooka, Daisuke / Okuzaki, Daisuke / Ikawa, Masahito / Kikutani, Hitoshi / Sakakibara, Shuhei

International immunology

2022 Volume 34, Issue 4, Page(s) 225

Language	English
Publishing date	2022-05-04
Publishing country	England
Document type	Published Erratum
ZDB-ID	1013745-2
ISSN	1460-2377 ; 0953-8178
ISSN (online)	1460-2377
ISSN	0953-8178
DOI	10.1093/intimm/dxac007
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2619: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 70: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Contribution of viral mimics of cellular genes to KSHV infection and disease.

Sakakibara, Shuhei / Tosato, Giovanna

Viruses

2014 Volume 6, Issue 9, Page(s) 3472–3486

Abstract: Kaposi's sarcoma-associated herpesvirus (KSHV, also named Human herpesvirus 8 HHV-8) is the cause of Kaposi sarcoma (KS), the most common malignancy in HIV-infected individuals worldwide, primary effusion lymphoma (PEL) and multicentric Castleman disease ...

Abstract	Kaposi's sarcoma-associated herpesvirus (KSHV, also named Human herpesvirus 8 HHV-8) is the cause of Kaposi sarcoma (KS), the most common malignancy in HIV-infected individuals worldwide, primary effusion lymphoma (PEL) and multicentric Castleman disease (MCD). KSHV is a double-stranded DNA virus that encodes several homologues of cellular proteins. The structural similarity between viral and host proteins explains why some viral homologues function as their host counterparts, but sometimes at unusual anatomical sites and inappropriate times. In other cases, structural modification in the viral proteins can suppress or override the function of the host homologue, contributing to KSHV-related diseases. For example, viral IL-6 (vIL-6) is sufficiently different from human IL-6 to activate gp130 signaling independent of the α subunit. As a consequence, vIL-6 can activate many cell types that are unresponsive to cellular IL-6, contributing to MCD disease manifestations. Here, we discuss the molecular biology of KSHV homologues of cellular products as conduits of virus/host interaction with a focus on identifying new strategies for therapy of KS and other KSHV-related diseases.
MeSH term(s)	Castleman Disease/etiology ; Cytokines/genetics ; Cytokines/metabolism ; Herpesviridae Infections/complications ; Herpesviridae Infections/genetics ; Herpesviridae Infections/virology ; Herpesvirus 8, Human/genetics ; Humans ; Inflammation Mediators/metabolism ; Lymphoma, Primary Effusion/etiology ; NF-kappa B/metabolism ; Sarcoma, Kaposi/etiology ; Tumor Suppressor Protein p53/metabolism
Chemical Substances	Cytokines ; Inflammation Mediators ; NF-kappa B ; Tumor Suppressor Protein p53
Language	English
Publishing date	2014-09-19
Publishing country	Switzerland
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v6093472
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Single-cell RNA-seq analysis identifies distinct myeloid cells in a case with encephalitis temporally associated with COVID-19 vaccination.

Ishikawa, Masakazu / Shimada, Yuki / Ozono, Tatsuhiko / Matsumoto, Hisatake / Ogura, Hiroshi / Kihara, Keigo / Mochizuki, Hideki / Okuno, Tatsusada / Sakakibara, Shuhei / Kinoshita, Makoto / Okuzaki, Daisuke

Frontiers in immunology

2023 Volume 14, Page(s) 998233

Abstract: Recently accumulating evidence has highlighted the rare occurrence of COVID-19 vaccination-induced inflammation in the central nervous system. However, the precise information on immune dysregulation related to the COVID-19 vaccination-associated ... ...

Abstract	Recently accumulating evidence has highlighted the rare occurrence of COVID-19 vaccination-induced inflammation in the central nervous system. However, the precise information on immune dysregulation related to the COVID-19 vaccination-associated autoimmunity remains elusive. Here we report a case of encephalitis temporally associated with COVID-19 vaccination, where single-cell RNA sequencing (scRNA-seq) analysis was applied to elucidate the distinct immune signature in the peripheral immune system. Peripheral blood mononuclear cells (PBMCs) were analyzed using scRNA-seq to clarify the cellular components of the patients in the acute and remission phases of the disease. The data obtained were compared to those acquired from a healthy cohort. The scRNA-seq analysis identified a distinct myeloid cell population in PBMCs during the acute phase of encephalitis. This specific myeloid population was detected neither in the remission phase of the disease nor in the healthy cohort. Our findings illustrate induction of a unique myeloid subset in encephalitis temporally associated with COVID-19 vaccination. Further research into the dysregulated immune signature of COVID-19 vaccination-associated autoimmunity including the cerebrospinal fluid (CSF) cells of central nervous system (CNS) is warranted to clarify the pathogenic role of the myeloid subset observed in our study.
MeSH term(s)	Humans ; COVID-19 Vaccines ; Leukocytes, Mononuclear ; Single-Cell Gene Expression Analysis ; COVID-19 ; Encephalitis ; Myeloid Cells ; Vaccination
Chemical Substances	COVID-19 Vaccines
Language	English
Publishing date	2023-02-23
Publishing country	Switzerland
Document type	Case Reports ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2023.998233
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Opposing roles of RUBCN isoforms in autophagy and memory B cell generation.

Tsai, Chao-Yuan / Sakakibara, Shuhei / Kuan, Yu-Diao / Omori, Hiroko / El Hussien, Maruwa Ali / Okuzaki, Daisuke / Lu, Shiou-Ling / Noda, Takeshi / Tabata, Keisuke / Nakamura, Shuhei / Yoshimori, Tamotsu / Kikutani, Hitoshi

Science signaling

2023 Volume 16, Issue 803, Page(s) eade3599

Abstract: RUBCN (also known as Rubicon) was originally identified as a negative regulator of autophagy, a process by which cells degrade and recycle damaged components or organelles and that requires the activity of the class III PI3K VPS34 and the mTORC1 protein ... ...

Abstract	RUBCN (also known as Rubicon) was originally identified as a negative regulator of autophagy, a process by which cells degrade and recycle damaged components or organelles and that requires the activity of the class III PI3K VPS34 and the mTORC1 protein complex. Here, we characterized the role of a shorter isoform, RUBCN
MeSH term(s)	Memory B Cells ; B-Lymphocytes ; Autophagy ; Protein Isoforms/genetics ; Mechanistic Target of Rapamycin Complex 1/genetics
Chemical Substances	Protein Isoforms ; Mechanistic Target of Rapamycin Complex 1 (EC 2.7.11.1)
Language	English
Publishing date	2023-09-19
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2417226-1
ISSN	1937-9145 ; 1945-0877
ISSN (online)	1937-9145
ISSN	1945-0877
DOI	10.1126/scisignal.ade3599
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Multiple tolerance checkpoints restrain affinity maturation of B cells expressing the germline precursor of a lupus patient-derived anti-dsDNA antibody in knock-in mice.

El Hussien, Marwa Ali / Tsai, Chao-Yuan / Satouh, Yuhkoh / Motooka, Daisuke / Okuzaki, Daisuke / Ikawa, Masahito / Kikutani, Hitoshi / Sakakibara, Shuhei

International immunology

2021 Volume 34, Issue 4, Page(s) 207–223

Abstract: Anti-dsDNA antibodies are a hallmark of systemic lupus erythematosus and are highly associated with its exacerbation. Cumulative evidence has suggested that somatic hypermutation contributes to the high-affinity reactivity of anti-dsDNA antibodies. Our ... ...

Abstract	Anti-dsDNA antibodies are a hallmark of systemic lupus erythematosus and are highly associated with its exacerbation. Cumulative evidence has suggested that somatic hypermutation contributes to the high-affinity reactivity of anti-dsDNA antibodies. Our previous study demonstrated that these antibodies are generated from germline precursors with low-affinity ssDNA reactivity through affinity maturation and clonal expansion in patients with acute lupus. This raised the question of whether such precursors could be subjected to immune tolerance. To address this, we generated a site-directed knock-in (KI) mouse line, G9gl, which carries germline-reverted sequences of the VH-DH-JH and Vκ-Jκ regions of patient-derived, high-affinity anti-dsDNA antibodies. G9gl heterozygous mice had a reduced number of peripheral B cells, only 27% of which expressed G9gl B-cell receptor (BCR). The remaining B cells harbored non-KI allele-derived immunoglobulin heavy (IgH) chains or fusion products of upstream mouse VH and the KI gene, suggesting that receptor editing through VH replacement occurred in a large proportion of B cells in the KI mice. G9gl BCR-expressing B cells responded to ssDNA but not dsDNA, and exhibited several anergic phenotypes, including reduced surface BCR and shortened life span. Furthermore, G9gl B cells were excluded from germinal centers (GCs) induced by several conditions. In particular, following immunization with methylated bovine serum albumin-conjugated bacterial DNA, G9gl B cells occurred at a high frequency in memory B cells but not GC B cells or plasmablasts. Collectively, multiple tolerance checkpoints prevented low-affinity precursors of pathogenic anti-dsDNA B cells from undergoing clonal expansion and affinity maturation in GCs.
MeSH term(s)	Animals ; Antibodies, Antinuclear ; B-Lymphocytes ; Germ Cells ; Humans ; Immune Tolerance/genetics ; Immunoglobulin Heavy Chains/genetics ; Lupus Erythematosus, Systemic/genetics ; Mice ; Receptors, Antigen, B-Cell
Chemical Substances	Antibodies, Antinuclear ; Immunoglobulin Heavy Chains ; Receptors, Antigen, B-Cell ; anti-dsDNA autoantibody
Language	English
Publishing date	2021-12-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1013745-2
ISSN	1460-2377 ; 0953-8178
ISSN (online)	1460-2377
ISSN	0953-8178
DOI	10.1093/intimm/dxab111
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2619: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 70: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Local production of broadly cross-reactive IgE against multiple fungal cell wall polysaccharides in patients with allergic fungal rhinosinusitis.

Haruna, Soichiro / Takeda, Kazuya / El-Hussien, Marwa Ali / Maeda, Yohei / Hayama, Masaki / Shikina, Takashi / Doi, Katsumi / Inohara, Hidenori / Kikutani, Hitoshi / Sakakibara, Shuhei

Allergy

2022 Volume 77, Issue 10, Page(s) 3147–3151

MeSH term(s)	Cell Wall ; Chronic Disease ; Fungal Polysaccharides ; Humans ; Immunoglobulin E ; Rhinitis, Allergic ; Sinusitis/microbiology
Chemical Substances	Fungal Polysaccharides ; Immunoglobulin E (37341-29-0)
Language	English
Publishing date	2022-06-27
Publishing country	Denmark
Document type	Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	391933-x
ISSN	1398-9995 ; 0105-4538
ISSN (online)	1398-9995
ISSN	0105-4538
DOI	10.1111/all.15413
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Uh III Zs.150: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MO 125: Show issues

Order via subito

Details ▾

Article ; Online: Viral interleukin-6: role in Kaposi's sarcoma-associated herpesvirus: associated malignancies.

Sakakibara, Shuhei / Tosato, Giovanna

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

2011 Volume 31, Issue 11, Page(s) 791–801

Abstract: Viral interleukin-6 (vIL-6) is a product of Kaposi's sarcoma-associated herpesvirus (KSHV) expressed in latently infected cells and to a higher degree during viral replication. A distinctive feature of vIL-6 is the ability to directly bind and activate ... ...

Abstract	Viral interleukin-6 (vIL-6) is a product of Kaposi's sarcoma-associated herpesvirus (KSHV) expressed in latently infected cells and to a higher degree during viral replication. A distinctive feature of vIL-6 is the ability to directly bind and activate gp130 signaling in the absence of other receptor subunits. Secretion of vIL-6 is generally poor, but vIL-6 can activate gp130 from inside the cell. Due to the wide cell distribution of gp130, vIL-6 has the potential to induce a wide range of biological effects. Expression of vIL-6 is variable in KSHV-associated Kaposi's sarcoma (KS), primary effusion lymphoma (PEL), multicentric Castleman's disease (MCD), and in a newly described MCD-like systemic inflammatory syndrome observed in human immunodeficiency virus-positive patients. PEL effusions usually contain vIL-6 at high concentrations; since vIL-6 induces vascular endothelial growth factor, vIL-6 likely contributes to vascular permeability and formation of PEL effusions. Lymph nodes affected with MCD contain vIL-6-positive cells, and vIL-6 levels rise in conjunction with flares of the disease and likely contribute to symptoms of inflammation. The development of vIL-6 inhibitors is a potentially important advance in the treatment of KSHV-associated malignancies where vIL-6 is expressed.
MeSH term(s)	Animals ; Castleman Disease/metabolism ; Castleman Disease/pathology ; Castleman Disease/virology ; Herpesvirus 8, Human/genetics ; Herpesvirus 8, Human/metabolism ; Humans ; Interleukin-6/genetics ; Interleukin-6/metabolism ; Lymphoma, Primary Effusion/metabolism ; Lymphoma, Primary Effusion/pathology ; Lymphoma, Primary Effusion/virology ; Sarcoma, Kaposi/metabolism ; Sarcoma, Kaposi/pathology ; Sarcoma, Kaposi/virology
Chemical Substances	Interleukin-6
Language	English
Publishing date	2011-07-18
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	1226675-9
ISSN	1557-7465 ; 1079-9907
ISSN (online)	1557-7465
ISSN	1079-9907
DOI	10.1089/jir.2011.0043
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1748: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Bystander inhibition of humoral immune responses by Epstein-Barr virus LMP1.

Tsai, Chao-Yuan / Sakakibara, Shuhei / Yasui, Teruhito / Minamitani, Takeharu / Okuzaki, Daisuke / Kikutani, Hitoshi

International immunology

2018 Volume 30, Issue 12, Page(s) 579–590

Abstract: Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP1), which mimics a constitutively active receptor, is required for viral transformation of primary B cells. LMP1 is expressed in EBV-infected germinal center (GC) B cells of immunocompetent ... ...

Abstract	Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP1), which mimics a constitutively active receptor, is required for viral transformation of primary B cells. LMP1 is expressed in EBV-infected germinal center (GC) B cells of immunocompetent individuals, suggesting that it may contribute to persistent EBV infection. In this study, we generated and analyzed mice that expressed LMP1 under the control of the CD19 or activation-induced cytidine deaminase (AID) promoter. Expression of LMP1 induced activation of B cells but severely inhibited their differentiation into antibody-secreting cells (ASCs) in vitro and GC B cells in vivo. LMP1-expressing (LMP1+) B cells not only suppressed the functions of wild-type (WT) B cells in in vitro co-culture, but also blocked differentiation of WT B cells into GC B cells and ASCs in immunized bone marrow chimeric mice. Microarray analysis revealed that the gene encoding indoleamine 2,3-dioxygenase 1 (IDO1), a major enzyme involved in the tryptophan metabolic process, was highly induced by LMP1. Either inhibition of IDO1 activity by methyl-l-tryptophan or knockout of Ido1 in LMP1+ B cells could rescue WT B cells from such suppression. IDO1-induced tryptophan consumption and production of tryptophan metabolites appeared to be responsible for inhibition of B-cell function. We conclude that LMP1 expression in antigen-committed B cells not only directly impairs GC B-cell differentiation, but also indirectly inhibits the functions of neighboring B cells, resulting in suppression of humoral immune responses. Such bystander inhibition by LMP1+ B cells may contribute to immune evasion by EBV.
MeSH term(s)	Animals ; B-Lymphocytes/immunology ; Cell Differentiation ; Herpesvirus 4, Human/immunology ; Immunity, Humoral/immunology ; Mice ; Mice, Transgenic ; Viral Matrix Proteins/immunology
Chemical Substances	EBV-associated membrane antigen, Epstein-Barr virus ; Viral Matrix Proteins
Language	English
Publishing date	2018-09-24
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1013745-2
ISSN	1460-2377 ; 0953-8178
ISSN (online)	1460-2377
ISSN	0953-8178
DOI	10.1093/intimm/dxy053
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2619: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 70: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Antibody feedback contributes to facilitating the development of Omicron-reactive memory B cells in SARS-CoV-2 mRNA vaccinees.

The Journal of experimental medicine

2022 Volume 220, Issue 2

Abstract: In contrast to a second dose of the SARS-CoV-2 mRNA vaccine, a third dose elicits potent neutralizing activity against the Omicron variant. To address the underlying mechanism for this differential antibody response, we examined spike receptor-binding ... ...

Abstract	In contrast to a second dose of the SARS-CoV-2 mRNA vaccine, a third dose elicits potent neutralizing activity against the Omicron variant. To address the underlying mechanism for this differential antibody response, we examined spike receptor-binding domain (RBD)-specific memory B cells in vaccinated individuals. Frequency of Omicron-reactive memory B cells increased ∼9 mo after the second vaccine dose. These memory B cells show an altered distribution of epitopes from pre-second memory B cells, presumably due to an antibody feedback mechanism. This hypothesis was tested using mouse models, showing that an addition or a depletion of RBD-induced serum antibodies results in a concomitant increase or decrease, respectively, of Omicron-reactive germinal center (GC) and memory B cells. Our data suggest that pre-generated antibodies modulate the selection of GC and subsequent memory B cells after the second vaccine dose, accumulating more Omicron-reactive memory B cells over time, which contributes to the generation of Omicron-neutralizing antibodies elicited by the third vaccine dose.
MeSH term(s)	Animals ; Mice ; Humans ; COVID-19 Vaccines ; Feedback ; Memory B Cells ; SARS-CoV-2 ; COVID-19/prevention & control ; RNA, Messenger ; Antibodies, Neutralizing ; Antibodies, Viral
Chemical Substances	COVID-19 Vaccines ; RNA, Messenger ; Antibodies, Neutralizing ; Antibodies, Viral
Language	English
Publishing date	2022-12-13
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	218343-2
ISSN	1540-9538 ; 0022-1007
ISSN (online)	1540-9538
ISSN	0022-1007
DOI	10.1084/jem.20221786
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ua VI Zs.107: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MG 45: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito