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Book ; Online: Chapter Sorption Detoxification as an Addition to Conventional Therapy of Acute Radiation Sickness and Iatrogenic Leukopenia

Sarnatskaya, V. V. / Snezhkova, E. A. / Chekhun, Vasyl F. / Vg, Nikolaev / Bilous, Anatoliy G. / Shevchuk, O. O. / Badakhivska, Kvitoslava I. / Sakhno, L. A.

2019

Keywords	Medical microbiology & virology ; leukopenia, ionizing irradiation, anti-cancer chemotherapy, granulocyte colony stimulating factor, hemosorption, enterosorption, application-sorption therapy
Size	1 Online-Ressource
Publisher	InTechOpen
Document type	Book ; Online
Note	English ; Open Access
HBZ-ID	HT021047889
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Article: [Fatty acid composition variability of rapeseed oil: classical selection and biotechnology].

Sakhno, L A

TSitologiia i genetika

2010 Volume 44, Issue 6, Page(s) 70–80

Abstract: The problems and achievements in the rapeseed Brassica napus L. var. oleifera breeding directed on the change of fatty acid composition in seed oil with the use of traditional and genetic engineering approaches are analyzed. It is noticed that the ... ...

Abstract	The problems and achievements in the rapeseed Brassica napus L. var. oleifera breeding directed on the change of fatty acid composition in seed oil with the use of traditional and genetic engineering approaches are analyzed. It is noticed that the combination of biotechnological workings out and methods of classical breeding is the optimum for the further improvement of rapeseed oil composition.
MeSH term(s)	Biotechnology/methods ; Brassica rapa/genetics ; Brassica rapa/growth & development ; Canola Oil ; Fatty Acids/analysis ; Fatty Acids, Monounsaturated ; Plant Oils/chemistry ; Plant Oils/standards ; Selection, Genetic
Chemical Substances	Canola Oil ; Fatty Acids ; Fatty Acids, Monounsaturated ; Plant Oils
Language	Russian
Publishing date	2010-11
Publishing country	Ukraine
Document type	Journal Article ; Review
ZDB-ID	390716-8
ISSN	0564-3783
ISSN	0564-3783
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Article: Comparative study of biochemical and morphological parameters in rats with Walker 256 and Walker 256/DOX carcinosarcoma.

Yushko, L A / Sarnatskaya, V V / Sakhno, L A / Hudenko, N V / Paziuk, L M / Maslenny, V N / Melnyk, V O / Nikolaev, V G

Experimental oncology

2021 Volume 43, Issue 1, Page(s) 21–25

Abstract: Aim: To evaluate the changes of some biochemical blood plasma parameters and morphological structure of the internal organs of rats with transplanted doxorubicin (DOX)-sensitive (Walker 256) and doxorubicin-resistant (Walker 256/DOX) strains of Walker ... ...

Abstract	Aim: To evaluate the changes of some biochemical blood plasma parameters and morphological structure of the internal organs of rats with transplanted doxorubicin (DOX)-sensitive (Walker 256) and doxorubicin-resistant (Walker 256/DOX) strains of Walker 256 carcinosarcoma. Materials and methods: The study was performed on female Wistar rats with transplanted Walker 256 or Walker 256/DOX and intact animals (control). On the 9 Results: Walker 256 growth, in comparison with Walker 256/DOX, is accompanied by more pronounced systemic effect on tumor-bearing rats. Uric acid concentration in the blood plasma of Walker 256 bearing rats was significantly (by 15.5%) higher than in Walker 256/DOX bearing rats. Aspartate aminotransferase activity in the Walker 256 group was significantly (by 107.2%) higher than in Walker 256/DOX group, but alanine aminotransferase activity was 58.5% lower. 56.7% decrease of alkaline phosphatase in rats with Walker 256, and 21% increase of this index in rats with Walker 256/DOX were observed. The growth of Walker 256 carcinosarcoma led to greater structural damage of the liver, kidneys and spleen in experimental animals compared with Walker 256/DOX strain. Conclusion: Tumor growth in rats with Walker 256/DOX leads to less pronounced changes in the biochemical parameters of rat blood plasma and morphological structure of internal organs compared with wild-type carcinosarcoma.
MeSH term(s)	Animals ; Carcinoma 256, Walker/blood ; Carcinoma 256, Walker/pathology ; Drug Resistance, Neoplasm/physiology ; Female ; Rats ; Rats, Wistar
Language	English
Publishing date	2021-03-10
Publishing country	Ukraine
Document type	Comparative Study ; Journal Article
ZDB-ID	2176927-8
ISSN	1812-9269 ; 0204-3564
ISSN	1812-9269 ; 0204-3564
DOI	10.32471/exp-oncology.2312-8852.vol-43-no-1.15636
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Article: Efficacy of dispersed fibrous carbon sorbent in treatment of malignant fungating wounds.

Sakhno, L A / Babenko, L P / Lazarenko, L M / Korotych, V G / Sarnatskaya, V V / Snezhkova, E A / Spivak, M Ya / Nikolaev, V G

Experimental oncology

2021 Volume 43, Issue 4, Page(s) 359–364

Abstract: Aim: To evaluate the influence of dispersed fibrous carbon sorbent (DFCS) on malignant fungating wounds (MFWs) in Guerin's carcinoma-bearing rats with an assessment of wound bacterial microflora.: Materials and methods: The study was performed on ... ...

Abstract	Aim: To evaluate the influence of dispersed fibrous carbon sorbent (DFCS) on malignant fungating wounds (MFWs) in Guerin's carcinoma-bearing rats with an assessment of wound bacterial microflora. Materials and methods: The study was performed on female Wistar rats inoculated subcutaneously with Guerin T8 carcinoma into the upper interscapular region. On day 20, the tumors were infected by injecting the suspension of S. aureus 8325-4. After 24 h, gauze dressings were applied daily to the wounds in the control group and DFCS in the experimental one. The state of the wounds was assessed by visual control with photo monitoring, odor control on a verbal rating scale and microbiological analysis of qualitative and quantitative characteristics of wound microbiota. Results: On the 3rd day after the start of dressings, the number of S. aureus of 8325-4 strain was significantly higher in the wounds of rats in control group than experimental one (5.14 ± 0.27 vs 3.43 ± 0.33 lg CFU/ml). The total number of Staphylococci in the DFCS-treated wounds did not differ statistically from the number of S. aureus 8325-4 strain whereas in control ones were higher by an order of magnitude. On the 10th day the total number of Staphylococci and S. aureus 8325-4 in particular, remained consistently high in the wounds of the control rats, while was almost five orders of magnitude lower and represented mainly by S. aureus 8325-4 in the experimental ones. The total number of aerobic and optionally anaerobic microorganisms was significantly lower as well. A greater variety of microorganisms in the gauze-covered wounds, as well as an their increased number were accompanied by enhancement of the wound odor from "noticeable" to "strong". There was an appearance of a "barely noticeable" odor in only one animal from the experimental group. Conclusion: The study has demonstrated the ability of DFCS to control substantially the bacterial microflora as well as malodor of MWFs in vivo. The results obtained can contribute to solving the problem of improving the quality of palliative care for patients with malignant and other chronic wounds.
MeSH term(s)	Animals ; Carbon ; Female ; Humans ; Neoplasms/complications ; Rats ; Rats, Wistar ; Staphylococcus aureus
Chemical Substances	Carbon (7440-44-0)
Language	English
Publishing date	2021-12-29
Publishing country	Ukraine
Document type	Journal Article
ZDB-ID	2176927-8
ISSN	1812-9269 ; 0204-3564
ISSN	1812-9269 ; 0204-3564
DOI	10.32471/exp-oncology.2312-8852.vol-43-no-4.16757
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Article: Adsorptive therapy as a modificator for tumor-host interaction.

Sakhno, L A / Sarnatskaya, V V / Yushko, L A / Snezhkova, E A / Bardakhivskaya, K I / Shevchuk, O O / Sidorenko, A S / Nikolaev, V G

Experimental oncology

2019 Volume 41, Issue 3, Page(s) 254–257

Abstract: The potential of one of the adsorption methods, enterosorption (ES), using the new generation of carbon adsorbents to correct the negative manifestations of tumor-host interaction in the framework of paraneoplastic syndrome (PNS) as well as systemic ... ...

Abstract	The potential of one of the adsorption methods, enterosorption (ES), using the new generation of carbon adsorbents to correct the negative manifestations of tumor-host interaction in the framework of paraneoplastic syndrome (PNS) as well as systemic toxicity of chemo- and radiation therapy, is discussed. The ES influence on the development of PNS was demonstrated in C57/BL6 mice with transplanted Lewis lung carcinoma. Two-week administration of carbon enterosorbents resulted in a significant suppression of metastasis and correction of tumor-related anemia, activation of granulocytic line in the bone marrow with nearly 3-fold enhancement of its mitotic activity. ES exerted a positive influence on the structural-morphologic indexes and regenerative potential of kidneys and liver, mitigated manifestations of oxidative stress, decreased the level of endogenous intoxication, increased resistance of erythrocyte membranes and decreased ligand loading of blood plasma transport proteins. The effect of ES on anticancer activity and toxic reactions of cisplatin (CP) was evaluated in Guerin carcinoma-bearing rats. ES reduced significantly creatinine and other kidney biochemical indexes elevated in the blood plasma of rats after CP treatment. ES attenuated dystrophic changes in the histological structure of internal organs (kidney, liver, spleen), caused by tumor growth and significantly aggravated under the influence of CP. Such changes were specially traced in the kidneys and well reflect the nephroprotective potential of ES. In rats irradiated with X-ray in sublethal dose, highly activated granulated carbonic enterosorbents facilitated the restoration of white blood cells and lymphocyte count. The results obtained confirm the insights of academician R.E. Kavetsky predicting the future of adsorptive detoxification with activated carbons in the treatment of cancer patients.
MeSH term(s)	Animals ; Antineoplastic Combined Chemotherapy Protocols/adverse effects ; Antineoplastic Combined Chemotherapy Protocols/therapeutic use ; Combined Modality Therapy/adverse effects ; Combined Modality Therapy/methods ; Disease Models, Animal ; Enterosorption/adverse effects ; Enterosorption/methods ; Humans ; Male ; Mice ; Neoplasms/complications ; Neoplasms/diagnosis ; Neoplasms/therapy ; Paraneoplastic Syndromes/therapy ; Treatment Outcome
Language	English
Publishing date	2019-09-27
Publishing country	Ukraine
Document type	Journal Article
ZDB-ID	2176927-8
ISSN	1812-9269 ; 0204-3564
ISSN	1812-9269 ; 0204-3564
DOI	10.32471/exp-oncology.2312-8852.vol-41-no-3.13576
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Article: Theoretical ground for adsorptive therapy of anthracyclines cardiotoxicity.

Shevchuk, O O / Posokhova, E A / Sakhno, L A / Nikolaev, V G

Experimental oncology

2012 Volume 34, Issue 4, Page(s) 314–322

Abstract: Anthracyclines play an important role in treatment of variety types of cancer due to their high effectiveness and broad spectrum of activity. However, a major limitation of their use is the dose-limiting cardiotoxicity. The inability to predict and ... ...

Abstract	Anthracyclines play an important role in treatment of variety types of cancer due to their high effectiveness and broad spectrum of activity. However, a major limitation of their use is the dose-limiting cardiotoxicity. The inability to predict and prevent anthracycline cardiotoxicity is in part due to the fact that the molecular and cellular mechanisms remain controversial and incompletely understood. This review focuses on the biochemical basis of the anthracyclines toxic cardiac effects and pharmacological measures to their treatment and preventing. We describe the theoretical substantiation of the enterosorption abilities for diminishing of cardiac damage.
MeSH term(s)	Animals ; Anthracyclines/adverse effects ; Anthracyclines/chemistry ; Heart/drug effects ; Heart Diseases/chemically induced ; Heart Diseases/therapy ; Humans
Chemical Substances	Anthracyclines
Language	English
Publishing date	2012-12
Publishing country	Ukraine
Document type	Journal Article ; Review
ZDB-ID	2176927-8
ISSN	1812-9269 ; 0204-3564
ISSN	1812-9269 ; 0204-3564
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Article: Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl-lipid desaturases and thermostable lichenase

Gerasymenko, I. M / Sakhno, L. A / Kyrpa, T. N / Ostapchuk, A. M / Hadjiev, T. A / Goldenkova-Pavlova, I. V / Sheludko, Y. V

Russian journal of plant physiology. 2015 May, v. 62, no. 3

2015

Abstract: We established transgenic lines of Nicotiana tabacum expressing hybrid genes of Synechocystis sp. PCC 6803 Δ12 (desA) acyllipid desaturase and Synechococcus vulcanus Δ9 (desC) acyllipid desaturase with or without sequence coding for transit peptide of ... ...

Abstract	We established transgenic lines of Nicotiana tabacum expressing hybrid genes of Synechocystis sp. PCC 6803 Δ12 (desA) acyllipid desaturase and Synechococcus vulcanus Δ9 (desC) acyllipid desaturase with or without sequence coding for transit peptide of Rubisco small subunit of Arabidopsis thaliana under control of a constitutive promoter. Reliable increase of linoleic acid portion (C18:2; Δ9,12) accompanied with decrease of α-linolenic acid (C18:3; Δ9,12,15) relative amount was detected for plants expressing hybrid desA::licBM3 gene. No reliable changes were detected in fatty acid profiles and unsaturation index of plants transformed with Δ9 desaturase gene desC::licBM3 lacking signals of intracellular targeting while expression of this gene with Arabidopsis thaliana Rubisco small subunit transit peptide sequence caused growth of C18:3 α-linolenic acid part simultaneously with reduction of C18:2 linoleic acid part, as well as increase of unsat-uration index. No changes in relative amount of Δ9-monounsaturated fatty acids were observed in any of studied lines. All plants expressing desaturase genes exhibited enhanced levels of superoxide dismutase (SOD) activity after cold treatment in contrast to control lines with suppressed SOD activity after cold treatment.
Keywords	Arabidopsis thaliana ; Nicotiana tabacum ; Synechococcus vulcanus ; Synechocystis ; alpha-linolenic acid ; cold treatment ; fatty acid composition ; gene expression ; genes ; genetically modified organisms ; licheninase ; linoleic acid ; ribulose-bisphosphate carboxylase ; superoxide dismutase ; thermal stability
Language	English
Dates of publication	2015-05
Size	p. 283-291.
Publishing place	Springer-Verlag
Document type	Article
ZDB-ID	1166808-8
ISSN	1608-3407 ; 1070-3292 ; 1021-4437
ISSN (online)	1608-3407
ISSN	1070-3292 ; 1021-4437
DOI	10.1134/S1021443715030073
Database	NAL-Catalogue (AGRICOLA)

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Article: Carbon adsorbents in oncology: achievements and perspectives.

Nikolaev, V G / Sakhno, L A / Snezhkova, E A / Sarnatskaya, V V / Yushko, L A

Experimental oncology

2011 Volume 33, Issue 1, Page(s) 2–8

Abstract: The results of own investigations and literature data are summarized to determine the place of the main methods of adsorption therapy in complex treatment of the patients with malignant tumors. New possibilities for the usage of new generation of carbon ... ...

Abstract	The results of own investigations and literature data are summarized to determine the place of the main methods of adsorption therapy in complex treatment of the patients with malignant tumors. New possibilities for the usage of new generation of carbon adsorbents and modern adsorptive technologies in cancer treatment are discussed.
MeSH term(s)	Adsorption/physiology ; Carbon/metabolism ; Carbon/therapeutic use ; Humans ; Neoplasms/therapy
Chemical Substances	Carbon (7440-44-0)
Language	English
Publishing date	2011-03
Publishing country	Ukraine
Document type	Journal Article ; Review
ZDB-ID	2176927-8
ISSN	1812-9269 ; 0204-3564
ISSN	1812-9269 ; 0204-3564
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Article: [Stable expression of promoterless bar gene in transgenic rape plants].

Sakhno, L A / Gocheva, E A / Komarnitskiĭ, I K / Kuchuk, N V

TSitologiia i genetika

2008 Volume 42, Issue 1, Page(s) 21–28

Abstract: Phosphinothricin resistant plants of two rapeseed (Brassica napus L. var. oleifera DC.) spring industrial cultivars were obtained by Agrobacterium tumefaciens leaf disk transformation. Vector constructions contained the promoterless coding sequence of ... ...

Abstract	Phosphinothricin resistant plants of two rapeseed (Brassica napus L. var. oleifera DC.) spring industrial cultivars were obtained by Agrobacterium tumefaciens leaf disk transformation. Vector constructions contained the promoterless coding sequence of phosphinothricin acetyltransferase (bar) gene located between two inverted lox-sites (elements of Cre/lox recombination system of P1 phage) and selective neomycinphosphotransferase II gene (nptII). Integration of the alien genes was confirmed by the PCR analyses. Stable and linked inheritance of foreign genes in T1 and T2 progeny was shown.
MeSH term(s)	Acetyltransferases/genetics ; Agrobacterium tumefaciens/pathogenicity ; Aminobutyrates/pharmacology ; Brassica napus/genetics ; Brassica napus/growth & development ; Brassica napus/microbiology ; Gene Expression ; Genes, Plant ; Genetic Vectors ; Herbicide Resistance/genetics ; Plant Leaves/genetics ; Plant Leaves/growth & development ; Plant Leaves/microbiology ; Plants, Genetically Modified ; Polymerase Chain Reaction ; Promoter Regions, Genetic ; Recombination, Genetic
Chemical Substances	Aminobutyrates ; phosphinothricin (51276-47-2) ; Acetyltransferases (EC 2.3.1.-) ; phosphinothricin N-acetyltransferase (EC 2.3.1.-)
Language	Russian
Publishing date	2008-01
Publishing country	Ukraine
Document type	English Abstract ; Journal Article
ZDB-ID	390716-8
ISSN	0564-3783
ISSN	0564-3783
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Article ; Online: Binding Potency of Heparin Immobilized on Activated Charcoal for DNA Antibodies.

Snezhkova, E A / Tridon, A / Evrard, B / Nikolaev, V G / Uvarov, V Yu / Tsimbalyuk, R S / Ivanuk, A A / Komov, V V / Sakhno, L A

Bulletin of experimental biology and medicine

2016 Volume 160, Issue 4, Page(s) 444–447

Abstract: In vitro experiments showed that heparin adsorbed on activated charcoal can bind antibodies raised against native and single-stranded DNA in a diluted sera pool with a high level of these DNA. Thus, heparin used as anticoagulant during hemosorption ... ...

Abstract	In vitro experiments showed that heparin adsorbed on activated charcoal can bind antibodies raised against native and single-stranded DNA in a diluted sera pool with a high level of these DNA. Thus, heparin used as anticoagulant during hemosorption procedure can demonstrate supplementary therapeutic activity resulting from its interaction with various agents involved in acute and chronic inflammatory reactions such as DNA- and RNA-binding substances, proinflammatory cytokines, complement components, growth factors, etc. Research and development of heparin-containing carbonic adsorbents for the therapy of numerous inflammatory and autoimmune diseases seems to be a promising avenue in hematology.
MeSH term(s)	Adsorption/physiology ; Animals ; Antibodies, Antinuclear/immunology ; Antibodies, Antinuclear/therapeutic use ; Anticoagulants/therapeutic use ; Autoimmune Diseases/therapy ; Charcoal/metabolism ; Charcoal/therapeutic use ; DNA, Single-Stranded/immunology ; Heparin/metabolism ; Heparin/therapeutic use ; Rabbits
Chemical Substances	Antibodies, Antinuclear ; Anticoagulants ; DNA, Single-Stranded ; Charcoal (16291-96-6) ; Heparin (9005-49-6)
Language	English
Publishing date	2016-02
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	390407-6
ISSN	1573-8221 ; 0007-4888 ; 0365-9615
ISSN (online)	1573-8221
ISSN	0007-4888 ; 0365-9615
DOI	10.1007/s10517-016-3192-x
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