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  1. Article ; Online: Real-time PCR quantification of Rhizoctonia solani AG-3 from soil samples.

    Salamone, Amy L / Okubara, Patricia A

    Journal of microbiological methods

    2020  Volume 172, Page(s) 105914

    Abstract: Rhizoctonia solani anastomosis group 3 (AG-3) causes several diseases of potato, including black scurf and stem canker, affecting potato production in the Skagit Valley, Washington, and around the world. Primers for a SYBR-Green II-based real-time ... ...

    Abstract Rhizoctonia solani anastomosis group 3 (AG-3) causes several diseases of potato, including black scurf and stem canker, affecting potato production in the Skagit Valley, Washington, and around the world. Primers for a SYBR-Green II-based real-time polymerase chain reaction (qPCR) assay were designed from sequences of the nuclear internal transcribed spacer (ITS) regions of fungal isolates of potato and onion from the Pacific Northwest, USA. The primers preferentially amplified R. solani AG-3 DNA, compared to DNA from R. solani AG-4, AG-5 and AG-8. In silico analysis of primer-template duplex stability indicated that the assay also will detect R. solani AG-3 isolates from pea and onion in Washington State and from diverse crop species around the world, but not R. solani AG-9 and AG-2-1. The assay was used to quantify R. solani AG-3 populations in pathogen-infested field soils after temporary flooding rotation, a practice found to be effective for reducing Sclerotinia sclerotiorum and R. solani AG-3 in potatoes in growth chamber studies. Population densities of the pathogen were not significantly reduced in saturated (flooded) soils relative to fallow. However, the qPCR approach was more sensitive and quantitative than the toothpick baiting method for diagnosis of these soil samples. Accurate detection and quantification of R. solani AG-3 in soil will facilitate the development of integrated management plans for Rhizoctonia diseases of potato.
    MeSH term(s) Bacterial Typing Techniques/methods ; DNA Primers ; Plant Diseases/microbiology ; Real-Time Polymerase Chain Reaction/methods ; Rhizoctonia/genetics ; Rhizoctonia/isolation & purification ; Soil ; Soil Microbiology ; Solanum tuberosum/microbiology ; Washington
    Chemical Substances DNA Primers ; Soil
    Language English
    Publishing date 2020-04-06
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 604916-3
    ISSN 1872-8359 ; 0167-7012
    ISSN (online) 1872-8359
    ISSN 0167-7012
    DOI 10.1016/j.mimet.2020.105914
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1849: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Real-time PCR quantification of Rhizoctonia solani AG-3 from soil samples

    Salamone, Amy L. / Okubara, Patricia A.

    Journal of microbiological methods. 2020 May, v. 172 p.105914-

    2020  

    Abstract: Rhizoctonia solani anastomosis group 3 (AG-3) causes several diseases of potato, including black scurf and stem canker, affecting potato production in the Skagit Valley, Washington, and around the world. Primers for a SYBR-Green II-based real-time ... ...

    Abstract Rhizoctonia solani anastomosis group 3 (AG-3) causes several diseases of potato, including black scurf and stem canker, affecting potato production in the Skagit Valley, Washington, and around the world. Primers for a SYBR-Green II-based real-time polymerase chain reaction (qPCR) assay were designed from sequences of the nuclear internal transcribed spacer (ITS) regions of fungal isolates of potato and onion from the Pacific Northwest, USA. The primers preferentially amplified R. solani AG-3 DNA, compared to DNA from R. solani AG-4, AG-5 and AG-8. In silico analysis of primer-template duplex stability indicated that the assay also will detect R. solani AG-3 isolates from pea and onion in Washington State and from diverse crop species around the world, but not R. solani AG-9 and AG-2-1. The assay was used to quantify R. solani AG-3 populations in pathogen-infested field soils after temporary flooding rotation, a practice found to be effective for reducing Sclerotinia sclerotiorum and R. solani AG-3 in potatoes in growth chamber studies. Population densities of the pathogen were not significantly reduced in saturated (flooded) soils relative to fallow. However, the qPCR approach was more sensitive and quantitative than the toothpick baiting method for diagnosis of these soil samples. Accurate detection and quantification of R. solani AG-3 in soil will facilitate the development of integrated management plans for Rhizoctonia diseases of potato.
    Keywords Allium cepa ; DNA ; Pisum sativum ; Rhizoctonia ; Sclerotinia sclerotiorum ; Thanatephorus cucumeris ; baiting ; computer simulation ; crop production ; crop rotation ; fallow ; fungi ; growth chambers ; internal transcribed spacers ; onions ; pathogens ; population density ; potatoes ; quantitative polymerase chain reaction ; soil ; soil sampling ; stem cankers ; Washington (state) ; Rhizoctonia solani AG-3 ; Black scurf ; qPCR ; Temporary rotation flooding ; Solanum tuberosum
    Language English
    Dates of publication 2020-05
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 604916-3
    ISSN 1872-8359 ; 0167-7012
    ISSN (online) 1872-8359
    ISSN 0167-7012
    DOI 10.1016/j.mimet.2020.105914
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1849: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Clonostachys rosea, a potential biological control agent for Rhizoctonia solani AG-3 causing black scurf on potato

    Salamone, Amy L. / Gundersen, Babette / Inglis, Debra Ann

    Biocontrol science and technology. 2018 Sept. 2, v. 28, no. 9 p.895-900

    2018  

    Abstract: Rhizoctonia solani AG-3 forms sclerotia on potato tubers, reducing quality and marketability. Potato discs with sclerotia were exposed to saturated soil, and following toothpick baiting, hyphae of R. solani parasitised with mycelia that either coiled ... ...

    Abstract Rhizoctonia solani AG-3 forms sclerotia on potato tubers, reducing quality and marketability. Potato discs with sclerotia were exposed to saturated soil, and following toothpick baiting, hyphae of R. solani parasitised with mycelia that either coiled around or penetrated cell walls were observed. Similar parasitic behaviour was observed in dual cultures. Clonostachys rosea was identified as the mycoparasite by morphology and ITS sequencing. When co-inoculated onto stems grown from disease-free seed potatoes, tubers of the C. rosea + R. solani inoculated plants had significantly lower black scurf (P < 0.0001) and higher yield (P = 0.0002) than R. solani inoculated controls.
    Keywords Clonostachys rosea ; Thanatephorus cucumeris ; biological control agents ; cell walls ; disease-free plants ; disease-free seeds ; hyphae ; internal transcribed spacers ; mycelium ; planting seed ; potatoes ; sclerotia ; seed tubers ; soil ; stems ; Solanum tuberosum ; Gliocladium roseum ; Bionectria ochroleuca
    Language English
    Dates of publication 2018-0902
    Size p. 895-900.
    Publishing place Taylor & Francis
    Document type Article ; Online
    ZDB-ID 1084478-8
    ISSN 1360-0478 ; 0958-3157
    ISSN (online) 1360-0478
    ISSN 0958-3157
    DOI 10.1080/09583157.2018.1498063
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 5910: Show issues

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  4. Article ; Online: Rickettsia species identified in adult, host-seeking Dermacentor occidentalis (Acari: Ixodidae) from Baja California, Mexico, and Oregon and Washington, United States.

    Paddock, Christopher D / Zambrano, Maria L / Clover, James R / Ladd-Wilson, Stephen / Dykstra, Elizabeth A / Salamone, Amy / Kangiser, David / Ayres, Bryan N / Shooter, Savannah L / Karpathy, Sandor E / Kjemtrup, Anne M / Beati, Lorenza / Levin, Michael L / Lane, Robert S / Zazueta, Oscar E

    Journal of medical entomology

    2024  Volume 61, Issue 3, Page(s) 781–790

    Abstract: The Pacific Coast tick (Dermacentor occidentalis Marx, 1892) is a frequently encountered and commonly reported human-biting tick species that has been recorded from most of California and parts of southwestern Oregon, southcentral Washington, and ... ...

    Abstract The Pacific Coast tick (Dermacentor occidentalis Marx, 1892) is a frequently encountered and commonly reported human-biting tick species that has been recorded from most of California and parts of southwestern Oregon, southcentral Washington, and northwestern Mexico. Although previous investigators have surveyed populations of D. occidentalis for the presence of Rickettsia species across several regions of California, populations of this tick have not been surveyed heretofore for rickettsiae from Baja California, Oregon, or Washington. We evaluated 1,367 host-seeking, D. occidentalis adults collected from 2015 to 2022 by flagging vegetation at multiple sites in Baja California, Mexico, and Oregon and Washington, United States, using genus- and species-specific assays for spotted fever group rickettsiae. DNA of Rickettsia 364D, R. bellii, and R. tillamookensis was not detected in specimens from these regions. DNA of R. rhipicephali was detected in D. occidentalis specimens obtained from Ensenada Municipality in Baja California and southwestern Oregon, but not from Washington. All ompA sequences of R. rhipichephali that were amplified from individual ticks in southwestern Oregon were represented by a single genotype. DNA of the Ixodes pacificus rickettsial endosymbiont was amplified from specimens collected in southwestern Oregon and Klickitat County, Washington; to the best of our knowledge, this Rickettsia species has never been identified in D. occidentalis. Collectively, these data are consistent with a relatively recent introduction of Pacific Coast ticks in the northernmost extension of its recognized range.
    MeSH term(s) Animals ; Rickettsia/isolation & purification ; Rickettsia/genetics ; Dermacentor/microbiology ; Washington ; Oregon ; Female ; Mexico ; Male
    Language English
    Publishing date 2024-03-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 410635-0
    ISSN 1938-2928 ; 0022-2585
    ISSN (online) 1938-2928
    ISSN 0022-2585
    DOI 10.1093/jme/tjae023
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 303: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Factors Influencing Distribution of Coccidioides immitis in Soil, Washington State, 2016.

    Chow, Nancy A / Kangiser, David / Gade, Lalitha / McCotter, Orion Z / Hurst, Steven / Salamone, Amy / Wohrle, Ron / Clifford, Wayne / Kim, Sunkyung / Salah, Zainab / Oltean, Hanna N / Plumlee, Geoffrey S / Litvintseva, Anastasia P

    mSphere

    2021  Volume 6, Issue 6, Page(s) e0059821

    Abstract: Coccidioides immitis and Coccidioides posadasii are causative agents of Valley fever, a serious fungal disease endemic to regions with hot, arid climate in the United States, Mexico, and Central and South America. The environmental niche ... ...

    Abstract Coccidioides immitis and Coccidioides posadasii are causative agents of Valley fever, a serious fungal disease endemic to regions with hot, arid climate in the United States, Mexico, and Central and South America. The environmental niche of
    MeSH term(s) Coccidioides/genetics ; Coccidioides/isolation & purification ; Coccidioidomycosis/epidemiology ; Coccidioidomycosis/microbiology ; DNA, Fungal/chemistry ; DNA, Fungal/genetics ; Endemic Diseases ; Humans ; Real-Time Polymerase Chain Reaction ; Soil Microbiology ; Washington
    Chemical Substances DNA, Fungal
    Language English
    Publishing date 2021-11-03
    Publishing country United States
    Document type Journal Article
    ISSN 2379-5042
    ISSN (online) 2379-5042
    DOI 10.1128/mSphere.00598-21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Fungal diversity of marine biofilms on artificial reefs in the north-central Gulf of Mexico

    Salamone, Amy L / Allison K. Walker / Brent M. Robicheau

    Botanica marina. 2016 Sept. 17, v. 59, no. 5

    2016  

    Abstract: We present the first characterization of fungal community diversity of natural mixed-species biofilms on artificial marine reefs. Four artificial reefs in the Mississippi (MS) Sound, USA, representing low-profile (underwater) and high-profile ( ... ...

    Abstract We present the first characterization of fungal community diversity of natural mixed-species biofilms on artificial marine reefs. Four artificial reefs in the Mississippi (MS) Sound, USA, representing low-profile (underwater) and high-profile (periodically air-exposed) conditions were sampled every 3 months over a 23-month period to investigate changes in fungal diversity within reef biofilms. Fungal presence was assessed via PCR amplification of the internal transcribed spacer (ITS) region of fungal ribosomal DNA, and by terminal restriction fragment length polymorphism (T-RFLP) analysis of fungal ITS regions – the latter being used to track variation in fungal community structure with respect to season, location, and reef profile type. Fungal communities were also characterized taxonomically through both morphological identification and phylogenetic comparisons of ITS gene sequences, with 36 fungal genera cultured from reef biofilms. Using a multivariate statistical approach, significant temporal and spatial differences in fungal biofilm communities were detected. High-profile reefs differed significantly in biofilm fungal community composition across the 10 sampling periods. This assessment of marine fungal biofilm communities over time provides novel insights into the fungal diversity present on artificial reefs in an understudied region, the north-central Gulf of Mexico.
    Keywords artificial reefs ; biofilm ; community structure ; fungal communities ; fungi ; internal transcribed spacers ; phylogeny ; polymerase chain reaction ; reefs ; restriction fragment length polymorphism ; ribosomal DNA ; statistical analysis ; transcription (genetics) ; Gulf of Mexico ; Mississippi
    Language English
    Dates of publication 2016-0917
    Size p. 291-305.
    Publishing place De Gruyter
    Document type Article
    ZDB-ID 1475447-2
    ISSN 1437-4323 ; 0006-8055
    ISSN (online) 1437-4323
    ISSN 0006-8055
    DOI 10.1515/bot-2016-0032
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