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  1. Article ; Online: Ultrasound-Guided Minimally Invasive Autopsy of Respiratory Muscles as a Safe and Cost-Effective Technique in COVID-19 Pandemic Era.

    Nucci, Ricardo Aparecido Baptista / Dolhnikoff, Marisa / Saldiva, Paulo Hilário do Nascimento / Jacob-Filho, Wilson

    Acta cytologica

    2021  Volume 65, Issue 3, Page(s) 276–278

    MeSH term(s) Autopsy/economics ; Autopsy/methods ; COVID-19/epidemiology ; COVID-19/prevention & control ; COVID-19/virology ; Cost-Benefit Analysis ; Humans ; Pandemics ; Respiratory Muscles/pathology ; SARS-CoV-2/isolation & purification ; SARS-CoV-2/physiology ; Ultrasonography/methods
    Language English
    Publishing date 2021-03-09
    Publishing country Switzerland
    Document type Letter
    ZDB-ID 80003-x
    ISSN 1938-2650 ; 0001-5547
    ISSN (online) 1938-2650
    ISSN 0001-5547
    DOI 10.1159/000514222
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  2. Article ; Online: Systemic dengue infection associated with a new dengue virus type 2 introduction in Brazil - a case report.

    Cunha, Marielton Dos Passos / Duarte-Neto, Amaro Nunes / Pour, Shahab Zaki / Hajjar, Ludhmila Abrahão / Frassetto, Fernando Pereira / Dolhnikoff, Marisa / Saldiva, Paulo Hilario do Nascimento / Zanotto, Paolo Marinho de Andrade

    BMC infectious diseases

    2021  Volume 21, Issue 1, Page(s) 311

    Abstract: Background: Dengue infection is caused by an arbovirus with a wide range of presentations, varying from asymptomatic disease to unspecific febrile illness and haemorrhagic syndrome with shock, which can evolve to death. In Brazil, the virus circulates ... ...

    Abstract Background: Dengue infection is caused by an arbovirus with a wide range of presentations, varying from asymptomatic disease to unspecific febrile illness and haemorrhagic syndrome with shock, which can evolve to death. In Brazil, the virus circulates since the 1980s with many introductions of new serotypes, genotypes, and lineages since then. Here we report a fatal case of dengue associated with a Dengue virus (DENV) lineage not detected in the country until now.
    Case presentation: The patient, a 58-year-old man arrived at the hospital complaining of fever and severe abdominal pain due to intense gallbladder edema, mimicking acute abdomen. After 48 h of hospital admission, he evolved to refractory shock and death. DENV RNA was detected in all tissues collected (heart, lung, brain, kidney, spleen, pancreas, liver, and testis). Viral sequencing has shown that the virus belongs to serotype 2, American/Asian genotype, in a new clade, which has never been identified in Brazil before. The virus was phylogenetically related to isolates from central America [Puerto Rico (2005-2007), Martinique (2005), and Guadeloupe (2006)], most likely arriving in Brazil from Puerto Rico.
    Conclusion: In summary, this was the first fatal documented case with systemic dengue infection associated with the new introduction of Dengue type 2 virus in Brazil during the 2019 outbreak.
    MeSH term(s) Brain/virology ; Brazil ; Dengue/diagnosis ; Dengue/virology ; Dengue Virus/classification ; Dengue Virus/genetics ; Dengue Virus/isolation & purification ; Gallbladder/pathology ; Genotype ; Heart/virology ; Humans ; Lung/virology ; Male ; Middle Aged ; Phylogeny ; RNA, Viral/metabolism
    Chemical Substances RNA, Viral
    Language English
    Publishing date 2021-04-01
    Publishing country England
    Document type Case Reports ; Journal Article
    ZDB-ID 2041550-3
    ISSN 1471-2334 ; 1471-2334
    ISSN (online) 1471-2334
    ISSN 1471-2334
    DOI 10.1186/s12879-021-05959-2
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  3. Article ; Online: Ultrasound assessment of pulmonary fibroproliferative changes in severe COVID-19: a quantitative correlation study with histopathological findings.

    de Almeida Monteiro, Renata Aparecida / Duarte-Neto, Amaro Nunes / Ferraz da Silva, Luiz Fernando / de Oliveira, Ellen Pierre / do Nascimento, Ellen Caroline Toledo / Mauad, Thais / Saldiva, Paulo Hilário do Nascimento / Dolhnikoff, Marisa

    Intensive care medicine

    2021  Volume 47, Issue 2, Page(s) 199–207

    Abstract: Purpose: This study was designed to evaluate the usefulness of lung ultrasound (LUS) imaging to characterize the progression and severity of lung damage in cases of COVID-19.: Methods: We employed a set of combined ultrasound parameters and ... ...

    Abstract Purpose: This study was designed to evaluate the usefulness of lung ultrasound (LUS) imaging to characterize the progression and severity of lung damage in cases of COVID-19.
    Methods: We employed a set of combined ultrasound parameters and histopathological images obtained simultaneously in 28 patients (15 women, 0.6-83 years) with fatal COVID-19 submitted to minimally invasive autopsies, with different times of disease evolution from initial symptoms to death (3-37 days, median 18 days). For each patient, we analysed eight post-mortem LUS parameters and the proportion of three histological patterns (normal lung, exudative diffuse alveolar damage [DAD] and fibroproliferative DAD) in eight different lung regions. The relationship between histopathological and post-mortem ultrasonographic findings was assessed using various statistical approaches.
    Results: Statistically significant positive correlations were observed between fibroproliferative DAD and peripheral consolidation (coefficient 0.43, p = 0.02) and pulmonary consolidation (coefficient 0.51, p = 0.005). A model combining age, time of evolution, sex and ultrasound score predicted reasonably well (r = 0.66) the proportion of pulmonary parenchyma with fibroproliferative DAD.
    Conclusion: The present study adds information to previous studies related to the use of LUS as a tool to assess the severity of acute pulmonary damage. We provide a histological background that supports the concept that LUS can be used to characterize the progression and severity of lung damage in severe COVID-19.
    MeSH term(s) Adolescent ; Adult ; Aged ; Aged, 80 and over ; COVID-19/diagnostic imaging ; COVID-19/pathology ; Child ; Child, Preschool ; Correlation of Data ; Female ; Humans ; Infant ; Lung/diagnostic imaging ; Lung/pathology ; Male ; Middle Aged ; Ultrasonography ; Young Adult
    Language English
    Publishing date 2021-01-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80387-x
    ISSN 1432-1238 ; 0340-0964 ; 0342-4642 ; 0935-1701
    ISSN (online) 1432-1238
    ISSN 0340-0964 ; 0342-4642 ; 0935-1701
    DOI 10.1007/s00134-020-06328-4
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  4. Article ; Online: Ultrasound-guided minimally invasive autopsies: A protocol for the study of pulmonary and systemic involvement of COVID-19.

    Monteiro, Renata Aparecida de Almeida / Duarte-Neto, Amaro Nunes / Silva, Luiz Fernando Ferraz da / Oliveira, Ellen Pierre de / Filho, Jair Theodoro / Santos, Glaucia Aparecida Bento Dos / Oliveira, Ilka Regina Souza de / Mauad, Thais / Saldiva, Paulo Hilário do Nascimento / Dolhnikoff, Marisa

    Clinics (Sao Paulo, Brazil)

    2020  Volume 75, Page(s) e1972

    MeSH term(s) Autopsy/methods ; Betacoronavirus ; COVID-19 ; Coronavirus Infections/pathology ; Humans ; Pandemics ; Pneumonia, Viral/pathology ; SARS-CoV-2 ; Ultrasonography, Interventional/methods
    Keywords covid19
    Language English
    Publishing date 2020-05-29
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2182801-5
    ISSN 1980-5322 ; 1807-5932
    ISSN (online) 1980-5322
    ISSN 1807-5932
    DOI 10.6061/clinics/2020/e1972
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  5. Book ; Online: Ultrasound-guided minimally invasive autopsies

    Monteiro, Renata Aparecida de Almeida / Duarte-Neto, Amaro Nunes / Silva, Luiz Fernando Ferraz da / Oliveira, Ellen Pierre de / Filho, Jair Theodoro / Santos, Glaucia Aparecida Bento dos / Oliveira, Ilka Regina Souza de / Mauad, Thais / Saldiva, Paulo Hilário do Nascimento / Dolhnikoff, Marisa

    Clinics v.75 2020

    A protocol for the study of pulmonary and systemic involvement of COVID-19

    2020  

    Keywords covid19
    Language English
    Publishing date 2020-01-01
    Publisher Faculdade de Medicina / USP
    Publishing country br
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: Ultrasound-guided minimally invasive autopsies: A protocol for the study of pulmonary and systemic involvement of COVID-19

    Monteiro, Renata Aparecida de Almeida / Duarte-Neto, Amaro Nunes / Silva, Luiz Fernando Ferraz da / Oliveira, Ellen Pierre de / Filho, Jair Theodoro / Santos, Glaucia Aparecida Bento Dos / Oliveira, Ilka Regina Souza de / Mauad, Thais / Saldiva, Paulo Hilário do Nascimento / Dolhnikoff, Marisa

    Clinics (Sao Paulo)

    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #518813
    Database COVID19

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  7. Article ; Online: Ultrasound-guided minimally invasive autopsies

    Monteiro, Renata Aparecida de Almeida / Duarte-Neto, Amaro Nunes / Silva, Luiz Fernando Ferraz da / Oliveira, Ellen Pierre de / Theodoro Filho, Jair / Santos, Glaucia Aparecida Bento dos / Oliveira, Ilka Regina Souza de / Mauad, Thais / Saldiva, Paulo Hilário do Nascimento / Dolhnikoff, Marisa

    Clinics; v.; e1972 ; Clinics; Vol. 75 (2020); e1972 ; Clinics; Vol 75 (2020); e1972 ; 1980-5322 ; 1807-5932

    A protocol for the study of pulmonary and systemic involvement of COVID-19

    2020  Volume 75

    Keywords covid19
    Language English
    Publishing date 2020-05-27
    Publisher Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
    Publishing country br
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article: Periodontal tissues are targets for Sars-Cov-2: a post-mortem study.

    Fernandes Matuck, Bruno / Dolhnikoff, Marisa / Maia, Gilvan V A / Isaac Sendyk, Daniel / Zarpellon, Amanda / Costa Gomes, Sara / Duarte-Neto, Amaro Nunes / Rebello Pinho, João Renato / Gomes-Gouvêa, Michele Soares / Sousa, Suzana C O M / Mauad, Thais / Saldiva, Paulo Hilário do Nascimento / Braz-Silva, Paulo H / da Silva, Luiz Fernando Ferraz

    Journal of oral microbiology

    2020  Volume 13, Issue 1, Page(s) 1848135

    Abstract: Background: ...

    Abstract Background:
    Language English
    Publishing date 2020-11-26
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2523919-3
    ISSN 2000-2297
    ISSN 2000-2297
    DOI 10.1080/20002297.2020.1848135
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  9. Article: Spatial analysis of urban violence based on emergency room data.

    Lima, Liliam Pereira de / Singer, Julio da Motta / Saldiva, Paulo Hilário do Nascimento

    Revista de saude publica

    2008  Volume 42, Issue 4, Page(s) 648–655

    Abstract: Objective: To estimate the spatial intensity of urban violence events using wavelet-based methods and emergency room data.: Methods: Information on victims attended at the emergency room of a public hospital in the city of São Paulo, Southeastern ... ...

    Abstract Objective: To estimate the spatial intensity of urban violence events using wavelet-based methods and emergency room data.
    Methods: Information on victims attended at the emergency room of a public hospital in the city of São Paulo, Southeastern Brazil, from January 1, 2002 to January 11, 2003 were obtained from hospital records. The spatial distribution of 3,540 events was recorded and a uniform random procedure was used to allocate records with incomplete addresses. Point processes and wavelet analysis technique were used to estimate the spatial intensity, defined as the expected number of events by unit area.
    Results: Of all georeferenced points, 59% were accidents and 40% were assaults. There is a non-homogeneous spatial distribution of the events with high concentration in two districts and three large avenues in the southern area of the city of São Paulo.
    Conclusions: Hospital records combined with methodological tools to estimate intensity of events are useful to study urban violence. The wavelet analysis is useful in the computation of the expected number of events and their respective confidence bands for any sub-region and, consequently, in the specification of risk estimates that could be used in decision-making processes for public policies.
    MeSH term(s) Adolescent ; Adult ; Aged ; Aged, 80 and over ; Brazil/epidemiology ; Child ; Child, Preschool ; Demography ; Emergency Service, Hospital/statistics & numerical data ; Female ; Geographic Information Systems ; Humans ; Infant ; Infant, Newborn ; Male ; Medical Records ; Middle Aged ; Public Policy ; Urban Population/statistics & numerical data ; Violence/statistics & numerical data ; Wounds and Injuries/epidemiology ; Young Adult
    Language English
    Publishing date 2008-08-08
    Publishing country Brazil
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 732179-x
    ISSN 1518-8787 ; 0034-8910
    ISSN (online) 1518-8787
    ISSN 0034-8910
    DOI 10.1590/s0034-89102008000400010
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  10. Article ; Online: The effect of air pollution on pneumonia-related emergency department visits in a region of extensive sugar cane plantations: a 30-month time-series study.

    Arbex, Marcos Abdo / Pereira, Luiz Alberto Amador / Carvalho-Oliveira, Regiane / Saldiva, Paulo Hilário do Nascimento / Braga, Alfésio Luís Ferreira

    Journal of epidemiology and community health

    2014  Volume 68, Issue 7, Page(s) 669–674

    Abstract: Background: In Brazil, many cities are surrounded by sugar cane plantations, and when these plantations are burnt prior to harvesting, millions of people are exposed to the smoke from these fires from May to November every year.: Methods: A daily ... ...

    Abstract Background: In Brazil, many cities are surrounded by sugar cane plantations, and when these plantations are burnt prior to harvesting, millions of people are exposed to the smoke from these fires from May to November every year.
    Methods: A daily time-series regression analysis was conducted in a city located in the sugar cane plantation region of São Paulo State, Brazil, between 1 February 2005 and 31 July 2007. The percentage increase in the number of pneumonia-related emergency department visits (PEDV) associated with a 10 µg/m(3) increase in the total suspended particles (TSP) concentration was measured, including any effects that were delayed for up to 6 days.
    Results: A total of 1505 PEDV (a median of two events per day) were analysed. During the burning period, there was an acute effect that began on the day of exposure and remained for 2 days. An increase of 6% (95% CI 2.4 to 9.9) in PEDV was observed for the 2 days following the TSP increase. This pattern and the size of the effect were similar to those observed for the whole period and also during the non-burning period.
    Conclusions: Increases in TSP concentrations were found to be associated with increased PEDV in a region affected by air pollution from sugar cane burning. This finding reinforces the need for polices and efforts to ban sugar cane burning prior to harvesting.
    MeSH term(s) Agriculture ; Air Pollution/adverse effects ; Brazil ; Databases, Factual ; Emergency Service, Hospital/utilization ; Environmental Exposure/adverse effects ; Humans ; Pneumonia/physiopathology ; Saccharum ; Smoke/adverse effects ; Time Factors
    Chemical Substances Smoke
    Language English
    Publishing date 2014-07
    Publishing country England
    Document type Journal Article
    ZDB-ID 391868-3
    ISSN 1470-2738 ; 0142-467X ; 0141-7681 ; 0143-005X
    ISSN (online) 1470-2738
    ISSN 0142-467X ; 0141-7681 ; 0143-005X
    DOI 10.1136/jech-2013-203709
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