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Article ; Online: Bio-Priming with Compatible Rhizospheric Microbes Enhances Growth and Micronutrient Uptake of Red Cabbage

Deepranjan Sarkar / Amitava Rakshit / Hanuman Prasad Parewa / Subhan Danish / Saleh Alfarraj / Rahul Datta

Land, Vol 11, Iss 536, p

2022 Volume 536

Abstract: Red cabbage is known as the millennium’s functional food, which has a lot of importance in our diet because of the health-promoting ingredients present in it. The current study investigated the synergistic relationship of rhizospheric-competent microbial ...

Abstract	Red cabbage is known as the millennium’s functional food, which has a lot of importance in our diet because of the health-promoting ingredients present in it. The current study investigated the synergistic relationship of rhizospheric-competent microbial agents ( Trichoderma harzianum , Pseudomonas fluorescens , and Bacillus subtilis ) in modulating the performance of red cabbage under the field conditions of Middle Gangetic Plains, India. Growth parameters were studied at three developmental stages, viz., pre-cupping, early head formation, and maturity. Our results suggested that the dual application of T . harzianum + P . fluorescens along with the 75% recommended dose of fertilizers (RDF) increased the number of leaves (24.6), leaf area (537.2 cm 2 ), root length (19.8 cm), and micronutrient uptake (Fe, Mn, and Cu) by head of the crop, whereas the co-inoculation of P . fluorescens and B . subtilis along with 75% RDF enhanced plant spread (39.0 cm), earliness (95.2 days), and Zn uptake. Maximum plant height (28.7 cm) and chlorophyll (SPAD, 77.3) were recorded in 100% RDF (120:60:60 kg ha −1 ) and the combination of T . harzianum + B . subtilis along with 75% RDF, respectively. Interestingly, consortium ( T . harzianum + P . fluorescens ) bio-primed plants recorded about 14% higher root length in comparison to plants receiving sole fertilizers. The regression analysis revealed a significant relationship of Fe and Mn uptake with chlorophyll (SPAD) and between Zn uptake and the earliness of the crop. The present study indicated that seedling bio-priming with the dual consortium of efficient bio-agents is a viable strategy to lessen our dependence on chemical fertilizers for improving red cabbage production.
Keywords	red cabbage ; rhizosphere engineering ; plant-microbe symbiosis ; growth parameters ; micronutrient uptake ; Agriculture ; S
Subject code	580
Language	English
Publishing date	2022-04-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Impact of different mulching treatments on weed flora and productivity of maize (Zea mays L.) and sunflower (Helianthus annuus L.).

Mubshar Hussain / Syed Nasir Abbas Shah / Muhammad Naeem / Shahid Farooq / Khawar Jabran / Saleh Alfarraj

PLoS ONE, Vol 17, Iss 4, p e

2022 Volume 0266756

Abstract: The concerns on weed control through herbicides are increasing due to their negative impacts on environment and human health. Therefore, alternative weed management methods are inevitable for sustainable crop production and lowering the negative ... ...

Abstract	The concerns on weed control through herbicides are increasing due to their negative impacts on environment and human health. Therefore, alternative weed management methods are inevitable for sustainable crop production and lowering the negative consequences of herbicides. Mulching is an environment-friendly weed management approach capable of substituting herbicides to significant extent. Therefore, this study evaluated the role of different mulching treatments on suppressing weed flora in maize (Zea mays L.) and sunflower (Helianthus annuus L.) crops. Furthermore, the impact of different mulching treatments on the productivity of both crops was also investigated. Three mulch treatments, i.e., plastic mulch (PLM), sorghum mulch (SM) and paper mulch (PM) along with two controls, i.e., weed-free (WF) and weedy-check (WC) were included in the study. Different mulch treatments significantly altered weed flora in both crops. The PLM and PM resulted in the highest suppression (43-47%) of weed flora compared to WC treatment in both crops. The highest and the lowest weed diversity was recorded for WC and WF treatments, respectively. Different allometric traits, i.e., leaf area index, crop growth rate and root length of both crops were significantly improved by PLM as compared to the WC. Overall, maize crop recorded higher density of individual and total weeds compared to sunflower with WC treatment. The density of individual and total weeds was significantly lowered by PLM compared to WC treatment in both crops. Similarly, higher growth and yield-related traits of both crops were noted with PLM compared to the rest of the mulching treatments. Results of the current study warrant that PLM could suppress weed flora and improve the productivity of both crops. However, PLM alone could not provide 100% control over weed flora; therefore, it should be combined with other weed management approaches for successful weed control in both crops.
Keywords	Medicine ; R ; Science ; Q
Subject code	571
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Survey of insect pests in the manuscripts library of Coptic museum in Egypt

Gawhara M.M. Abu El-Hassan / Saleh Alfarraj / Sulaiman A. Alharbi / Nagwa H. Atiya

Saudi Journal of Biological Sciences, Vol 28, Iss 9, Pp 5061-

2021 Volume 5064

Abstract: Museums are the main sources of cultural, political, economic, scientific and historic information in the communities. Pests in a museum, library or archive environment can cause serious damage to highly valuable and irreplaceable materials. A survey was ...

Abstract	Museums are the main sources of cultural, political, economic, scientific and historic information in the communities. Pests in a museum, library or archive environment can cause serious damage to highly valuable and irreplaceable materials. A survey was conducted in the Manuscripts Library of the Coptic Museum (Egypt) to determine the biodiversity of insect pests infest the place. Sampling were done monthly for a year (from October 2018 to September 2019) using sticky traps with a nontoxic sticky substance. The sticky traps were placed at the corners of the library, behind doors and on the windows edges. A total of 1047 specimens belonging to nine species under six families and five orders were collected and identified. The most abundant species was Monomorium pharaonic with a total of 639 collected specimens followed by Ochetellus glaber, Thermobia domestica, Gibbium psylloides, Anthrenus verbasci, Periplaneta Americana, Lasioderma serricorne, Liposcelis bostrychophila, Attagenus fasciatus with total number of 193, 62, 45, 39, 23, 21, 13, 12 collected specimens, respectively. The traps which sited in the corners of the library trapped 60% of the total recorded specimens.
Keywords	Survey ; Museum pests ; Antiquities ; Egypt ; Biology (General) ; QH301-705.5
Subject code	590
Language	English
Publishing date	2021-09-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Assessment of risk management and control measures against coronavirus disease

Khalil Ur Rehman / Shahla Andleeb / Saleh Alfarraj / Sulaiman Ali Alharbi / Adeel Mahmood

Saudi Journal of Biological Sciences, Vol 28, Iss 5, Pp 3013-

2021 Volume 3020

Abstract: This article presents the COVID-19 situation and control measures taken by the Government of Pakistan. Two waves of pandemic are faced globally and similar in the study area. We have investigated the risk management decision in two phases. Primarily, ... ...

Abstract	This article presents the COVID-19 situation and control measures taken by the Government of Pakistan. Two waves of pandemic are faced globally and similar in the study area. We have investigated the risk management decision in two phases. Primarily, strict lockdown was observed from March 2020 to July 2020 and smart lockdown was enforced from August 2020 to December 2020. It has been studied that during strict lockdown, COVID cases reduced gradually but reopening of institutes and smart lockdown strategy resulted gradual increase in confirmed cases and death rates. During first wave of COVID-19 in Pakistan, a total confirmed number of patients of COVID-19 were 263,496 till 18th of July 2020 with total deaths of 5,568 people and 204,276 recoveries, while total number of COVID-19 patients reached 555,511 till 9th of February 2021 with total deaths of 12,026 people. Province of Sindh was affected badly with total number of 251,434 COVID-19 cases followed by Punjab Province with total number of 161,347 COVID-19 till 9th of February 2020.
Keywords	Risk assessment ; Disease management ; Virus spread ; Pandemic ; Situation analysis ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2021-05-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Simultaneous determination of four endogenous steroids in bio matrices by LC-MS/MS

Premanand Ranganathan / V. Gunasekaran / Indrajeet Singhvi / Saleh Alfarraj / Salma Usmani / Mohammad Javed Ansari

Journal of King Saud University: Science, Vol 33, Iss 1, Pp 101245- (2021)

2021

Abstract: A simple sensitive and accessible ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry using AJS-ESI method is established and validated for the intended purpose of quantification of major cholesterol steroidal ... ...

Abstract	A simple sensitive and accessible ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry using AJS-ESI method is established and validated for the intended purpose of quantification of major cholesterol steroidal metabolites Corticosterone, 11α-Hydroxyprogesterone, Testosterone and Progesterone is extracted by Tertiary butyl methyl ether solvent from human plasma and analyzed using Infinity Lab Poroshell 120 EC-C18, (2.1 × 150 mm, 2.7 µm) column by gradient elution. The Eluent composed of 5 milli molar Ammonium formate in Methanol as eluent A and 5 mM Ammonium formate as eluent B and detection was done by AJS- ESI fitted tandem mass spectrometry in positive mode. The internal standard used is Caffeine. The quantification lower limits (LLOQ) are 0.200 ng per liter for all four steroids Corticosterone, 11α-Hydroxyprogesterone, Testosterone and Progesterone. The Calibration curve are linear over the respectively limits of 0.200 to 1000.000 ng per milliliter of Plasma for each analyte and quadric for Testosterone. This new LC-MS/MS method shows presentable precision & accuracy and provides satisfactory sensitive for the fulfillment of human pharmacokinetic studies.
Keywords	Progesterone ; 11α-Hydroxyprogesterone ; Corticosterone ; Testosterone ; Method validation and LCMSMS ; Science (General) ; Q1-390
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Parasites on the wing; two new records of marine chewing lice (Phthiraptera) on Brown booby (Suliformes

Eslam Adly / Mohamed Nasser / Saleh Alfarraj / Sulaiman Ali Alharbi / Sara Al-Ashaal

Journal of King Saud University: Science, Vol 33, Iss 5, Pp 101451- (2021)

Sulidae) from Egypt with notes on genus Pectinopygus/boobies phylogeny

2021

Abstract: Objectives: Little studies had been done on chewing lice of marine birds in the Middle East. Through this work, parasitic chewing lice of Brown Booby Sula leucogaster (Boddaert) in the Red Sea were recorded for the first time from Egypt. Methods: One ... ...

Abstract	Objectives: Little studies had been done on chewing lice of marine birds in the Middle East. Through this work, parasitic chewing lice of Brown Booby Sula leucogaster (Boddaert) in the Red Sea were recorded for the first time from Egypt. Methods: One brown booby was examined for chewing lice in Giftun Island, Hurghada. A total of 27 specimens of lice were collected from this bird and phylogenetic analysis of species of genus Pectinopygus associated boobies has been done based on NCBI data. Results and conclusions: Two species of marine chewing lice were identified: Eidmanniella albescens (Piaget, 1880) and Pectinopygus sulae (Rudow, 1869) diagnostic remarks for the two identified species, measurements, and material examined were provided through the manuscript. The final phylogenetic tree indicates the monophyletic origin of Pectinopygus spp. associated with boobies and their paraphyletic relation to other species of Pectinopygus that associated with other Suliformes. Also, independence of boobies’ speciation from chewing louse of genus Pectinopygus speciation. The present work forms a small step in a long way of studying marine chewing lice of Egypt and a better understanding of marine birds/chewing lice interaction.
Keywords	Egypt ; Red Sea ; New records ; Chewing lice ; Brown booby ; Science (General) ; Q1-390
Subject code	590
Language	English
Publishing date	2021-07-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Impact of Gamma Irradiation and Kale Leaf Powder on Amino Acid and Fatty Acid Profiles of Chicken Meat under Different Storage Intervals

Waseem Khalid / Muhammad Sajid Arshad / Gulzar Ahmad Nayik / Saleh Alfarraj / Mohammad Javed Ansari / Raquel P. F. Guiné

Molecules, Vol 27, Iss 8201, p

2022 Volume 8201

Abstract: The present study was planned to determine the effect of kale leaf powder and gamma rays on variations in the pH, amino acid and fatty acid profiles of chicken meat at different storage intervals. Significant changes ( p ≤ 0.05) in the pH, amino acid and ...

Abstract	The present study was planned to determine the effect of kale leaf powder and gamma rays on variations in the pH, amino acid and fatty acid profiles of chicken meat at different storage intervals. Significant changes ( p ≤ 0.05) in the pH, amino acid and fatty acid profiles of chicken meat following different treatments (KLP (1% and 2%) and gamma irradiation (3k Gy)) were reported at 0, 7 and 14 days of storage. The pH value of the chicken meat sample decreased with the addition of kale leaf powder, whereas the value increased following a gamma irradiation dose of 3 kGy and with the passage of time. During different storage intervals, the minimum reduction in the amino acid and fatty acid quantities in the chicken meat samples was reported after gamma irradiation treatment. However, with the addition of KLP, the amount of amino acids and fatty acids in the chicken meat samples increased. Conclusively, the pH was observed to be reduced in the meat following combined treatment (irradiation + KLP), whereas the 2% KLP treatment improved the amino acid and fatty acid profiles of the chicken samples.
Keywords	chicken meat ; gamma rays ; kale leaf powder ; amino acids ; fatty acids ; Organic chemistry ; QD241-441
Subject code	571
Language	English
Publishing date	2022-11-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Sero-RAPD makers based evaluation of chilli pepper germplasm for resistance to chilli veinal mottle virus

Sehrish Saba / Muhammad Ashfaq / Mirza Abid Mehmood / Furqan Ahmad / Saleh Alfarraj / Mohammad Javed Ansari / Amir Maqbool

Journal of King Saud University: Science, Vol 34, Iss 8, Pp 102303- (2022)

2022

Abstract: Background: Chilli veinal mottle virus is a remarkable threat to chilli crops worldwide including Pakistan. Methods: In the present study, 18 chilli pepper genotypes were catalogued against ChiVMV ATIPK isolate by using DAS-ELISA with virus-specific ... ...

Abstract	Background: Chilli veinal mottle virus is a remarkable threat to chilli crops worldwide including Pakistan. Methods: In the present study, 18 chilli pepper genotypes were catalogued against ChiVMV ATIPK isolate by using DAS-ELISA with virus-specific polyclonal antisera. Results: Out of 18 genotypes, four genotypes viz., Loungi pepper, Sanam, Red Pepper, and CV-1 were categorized as highly susceptible followed by three susceptible (Soofi, Royal Wonder, Pepper PLSQ) and four (Yolo wonder, Bhakkar Local, CIM-506, CR-25) were moderately susceptible. The plants of these genotypes exhibited characteristic symptoms of ChiVMV and accumulated a high level of virus titer. The rest of three (California wonder, Gola Peshawar, Red top) and four (CV-7, Hot Pepper, Doni Pepper, Ghotki) genotypes were grouped as resistant and moderately resistant, respectively. Three resistant and three highly susceptible genotypes were further appraised for genetic diversity by RAPD markers and a total of 75 bands were scored, 76% of them unveiled polymorphism with an amplification range of 5–9 bands. Genetic analysis revealed 44–78.67% similarities with Mean Similarity Index (MSI) ranging from 59.46 to 65.86%. Most of the primers were highly informative with PIC value fluctuated from 0.388 to 0.666, with an average value of 0.512. Cluster analysis further confirmed and clustered both groups of genotypes in a separate clade. Conclusion: It is concluded that genotypes; California Wonder and Red Top showed diverse response from other genotypes, not only based on symptoms and ELISA but also on markers' basis. Therefore, these genotypes could be useful as ChiVMV resistant sources in the chilli breeding program.
Keywords	Chilli ; RAPD ; Genetic diversity ; ELISA ; ChiVMV ; Science (General) ; Q1-390
Language	English
Publishing date	2022-11-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Deciphering waste bound nitrogen by employing psychrophillic Aporrectodea caliginosa and priming of coprolites by associated heterotrophic nitrifiers under high altitude Himalayas

Tahir Sheikh / Zahoor Baba / Ali Mohd Yatoo / Basharat Hamid / Sadaf Iqbal / Fehim Wani / Sabah Fatima / Saleh Alfarraj / Mohammad Javed Ansari

Scientific Reports, Vol 12, Iss 1, Pp 1-

2022 Volume 16

Abstract: Abstract Himalayan ecosystem is characterized by its fragile climate with rich repositories of biodiversity. Waste collection and disposal are becoming increasingly difficult due to topographical variations. Aporrectodea caligenosa, a versatile ... ...

Abstract	Abstract Himalayan ecosystem is characterized by its fragile climate with rich repositories of biodiversity. Waste collection and disposal are becoming increasingly difficult due to topographical variations. Aporrectodea caligenosa, a versatile psychrophillic soil dweller, is a useful biocatalyst with potent bio-augmented capability for waste treatment at low temperatures. Microcosm experiments were conducted to elucidate the comprehensive nature of biogenic nitrogen transformation to NH4 + and NO3 − produced by coupling of earthworm-microbes. Higher biogenic recovery of NH4 +-N from coprolites of garden soil (47.73 ± 1.16%) and Himalayan goat manure (86.32 ± 0.92%) with an increment of 14.12 and 47.21% respectively over their respective control (without earthworms) with a linear decline beyond 4th week of incubation was reported. NO3 –-N recovery progressively sustained in garden soil and goat manure coprolites during entire incubation with highest 81.81 ± 0.45 and 87.20 ± 1.08 µg-N g−1dry weight recorded in 6th and 5th week of incubation respectively and peak increments as 38.58 and 53.71% relative to respective control (without earthworms). Declined NH4 +–N in coprolites at low temperature (15.0 ± 2.0 °C) evidenced increased nitrification rates by taking over the process by abundant nitrifying microbes. Steady de-nitrification with progressive incubation on an average was 16.95 ± 0.46 ng-N g−1 per week and 21.08 ± 0.87 ng-N g−1 per week compared to 14.03 ± 0.58 ng-N g−1 per week and 4.50 ± 0.31 ng-N g−1 per week in respective control treatments. Simultaneous heterotrophic nitrification and aerobic denitrification (SHNAD) was found to be a prominent bioprocess at low temperature that resulted in high and stable total nitrogen and nitrate accumulation from garden soil and goat manure with relative recovery efficiency of 11.12%, 14.97% and 14.20%; 19.34%. A. caligenosa shows promising prospects for mass applicability in biogenic N removal from manure of Himalayan goat.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Infestation rate and cardinal directional preference of pistachio twig borer [Kermania pistaciella Amsel. (Lepidoptera

Mehmet Mamay / Ceyhan Sönmez / Yunzhou Li / Çetin Mutlu / Imran Akhtar / Rehana Bibi / Saleh Alfarraj / Mohammad Javed Ansari / Tomas Vyhnanek

Journal of King Saud University: Science, Vol 34, Iss 4, Pp 102025- (2022)

Tineidae)]

2022

Abstract: Background: Pistachio twig borer [Kermania pistaciella Amsel, 1964 (Lepidoptera: Tineidae)] is one of the most important pests of pistachio that opens galleries in the shoots and causes blind and sparse-grained clusters and all these damage fruit ... ...

Abstract	Background: Pistachio twig borer [Kermania pistaciella Amsel, 1964 (Lepidoptera: Tineidae)] is one of the most important pests of pistachio that opens galleries in the shoots and causes blind and sparse-grained clusters and all these damage fruit clusters. However, the infestation rates and cardinal direction preference are unknown for the pest. Methods: This study determined infestation rate and cardinal direction preference of K. pistaciella in pistachio orchards during 2019 and 2020. The orchards located in in 25 different locations, i.e., Birecik, Bozova, Ceylanpınar, Eyyübiye, Halfeti, Haliliye, Hilvan and Karaköprü districts Şanlıurfa province Turkey. Surveys were carried out in three or four orchards representing each district included in the study. Twenty-five trees were randomly selected from each orchard and one shoot from each side of the tree was collected (100 shoots per orchard) for determining infestation and cardinal directional preference. A total 2500 shoots were observed from the study area and infestation was recorded. Results: The highest infestation rate was recorded for Yaslıca village in Bozova district (70%) during 2019, and Göklü village of Halfeti and Akziyaret village in Karaköprü district (29%) during 2020. The lowest infestation rate (2%) was noted for Akrepli village of Ceylanpınar district during both years of the study. Bozova and Halfeti were the most infested districts compared to the rest of the districts included in the study. The highest and the lowest infestation rate was recorded for north and west directions, respectively during both years of study. The average of two years data revealed that infestation rate for the shoots collected from the north direction was 23.68%, while it 20.33% for the shoots collected form west direction. Statistical analysis revealed non-significant difference for infestation rates of the shoots collected from different directions (χ2 = 0,9468; p = 0,8141). Conclusion: The results revealed that all tree directions should be considered for the ...
Keywords	Kermania pistaciella ; Pistachio ; Damage ; Infestation ; Preference ; Science (General) ; Q1-390
Subject code	580
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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