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  1. Article ; Online: Bagarius bagarius, and Eichhornia crassipes are suitable bioindicators of heavy metal pollution, toxicity, and risk assessment

    Mohammad Mahamood / Farmanur Rahman Khan / Farhana Zahir / Mehjbeen Javed / Saleh S. Alhewairini

    Scientific Reports, Vol 13, Iss 1, Pp 1-

    2023  Volume 12

    Abstract: Abstract Water quality index (WQI) of Narora channel and health of endemic fish Bagarius bagarius and plant Eichhornia crassipes, district Bulandshahar, Uttar Pradesh, India were studied. Among the physicochemical properties of water, pH, D.O, Cr, Fe, Ni, ...

    Abstract Abstract Water quality index (WQI) of Narora channel and health of endemic fish Bagarius bagarius and plant Eichhornia crassipes, district Bulandshahar, Uttar Pradesh, India were studied. Among the physicochemical properties of water, pH, D.O, Cr, Fe, Ni, and Cd were above the recommended standards. These factors lead to high WQI (4124.83), indicating poor quality and not suitable for drinking and domestic usage. In fish tissues, the highest metal load was reported in the liver (58.29) and the lowest in the kidney (33.73). Heavy metals also cause a lowering of condition indices. As expected, decreased serum protein (− 63.41%) and liver glycogen (− 79.10%) were recorded in the exposed fish. However, blood glucose (47.22%) and serum glycogen (74.69%) showed elevation. In the plant, roots (21.50) contained the highest, and leaves (16.87) had the lowest heavy metal load. Bioaccumulation factor (BAF) > 1, indicates hyperaccumulation of all metals. E. crassipes roots showed the highest translocation factor (TF) > 1 for Ni (1.57) and Zn (1.30). The high mobility factor (MF) reflected the suitability of E. crassipes for phytoextraction of Mn, Cd, Zn, Fe, Ni, and Cu. Moreover, Bagarius sp. consumption could not pose any non-cancer risk. Although, lower cancer risk can be expected from Ni and Cr.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2023-02-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Characterization and bioactivities of M. arvensis, V. officinalis and P. glabrum

    Syed Aizaz Ali Shah / Naveeda Akhtar Qureshi / Muhammad Zahid Qureshi / Saleh S. Alhewairini / Anber Saleem / Adnan Zeb

    Saudi Journal of Biological Sciences, Vol 30, Iss 6, Pp 103646- (2023)

    In-silico modeling of V. officinalis as a potential drug source

    2023  

    Abstract: In current study the pharmaceutically active herbs was used against coccidiosis, caused by a protozoan: Eimeria, lead to $ 3 billion loss annually. The aqueous and methanolic extracts of whole plants were applied in-vitro to assess sporulation inhibition ...

    Abstract In current study the pharmaceutically active herbs was used against coccidiosis, caused by a protozoan: Eimeria, lead to $ 3 billion loss annually. The aqueous and methanolic extracts of whole plants were applied in-vitro to assess sporulation inhibition (spi) assay and calculated the inhibitory concentration (IC50). For in-vivo study 9 groups of 14 day old broiler chicks were infected with Eimeria tenella and three groups were treated different concentrations of methanolic extracts of Verbena officinalis and Polygonum glabrum post infection. The mean weight gain, oocyst count, diarrhea, biochemical tests, hematology, and histopathology of all groups were analyzed. The herbs were characterized by antioxidant assay, phytochemical screening, Fourier transmission and infrared (FT-IR), Ultra Violet-visible (UV–Vis) spectroscopy and Gas chromatography and mass spectroscopy (GC–MS). The GC–MS identified phyto-compounds of V. officinalis were docked with S-Adenosyl methionine (SAM) synthetase. The in-vitro study revealed that V. officinalis and P. glabrum have minimum IC50 of 0.14 and 12 mg/ml respectively. The in-vivo experiment showed that V. officinalis had significantly high anticoccidial potential with significant hematological profile like drug treated controls. The histology of treated chicks also showed recovery in the studied tissues. The antioxidant assay showed that V. officinalis have 4.19U/mg Superoxide dismutase (SOD) and 33.96 µM/mg Glutathione (GSH) quantities. The chemical characterization confirmed the presence of large number of organic compounds, however Flavonoids found only in V. officinalis, which suggests the anticoccidial potential of V. officinalis because flavonoids as antagonist of thiamine (Prinzo, 1999), because it promotes the carbohydrate synthesis required. Strychane, 1-acetyl-20a-hydroxy-16-methylene has best binding of with target protein with lowest binding score (-6.4 Kcal/mol), suggests its anticoccidial potential in poultry.
    Keywords Avain coccidiosis ; Eimeria tenella ; ROSS 308 broiler chicks ; Verbena officinalis ; Polygonum glabrum ; Biology (General) ; QH301-705.5
    Subject code 540
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article: A Fast and Easy QuEChERS-DLLME Method Combined with GC-MS for Ethion and Bifenthrin Residues Determination and Study of Their Dissipation Dynamics in Palm Dates

    Abdel Ghani, Sherif B / Saleh S. Alhewairini / Svetlana Hrouzková

    Food analytical methods. 2018 Dec., v. 11, no. 12

    2018  

    Abstract: New fast and efficient analytical method based on QuEChERS followed by dispersive liquid-liquid microextraction (DLLME) was developed for extraction and pre-concentration of residues of ethion and bifenthrin insecticide/acaricides in palm date matrix. GC- ...

    Abstract New fast and efficient analytical method based on QuEChERS followed by dispersive liquid-liquid microextraction (DLLME) was developed for extraction and pre-concentration of residues of ethion and bifenthrin insecticide/acaricides in palm date matrix. GC-MS was used for quantification of the pesticides. The developed method was validated according to the European regulations’ requirements. Method accuracy was 102.2% and 96.1% with a precision of 8.4% and 4.3% (intra-day) and 7.9 and 5.1% (inter-day) for ethion and bifenthrin, respectively. The validated QuEChERS and QuEChERS-DLLME methods were compared. Applying DLLME for preconcentration of pesticides lowered the limit of quantification nearly 20 times. By adding the DLLME step using chlorobenzene as extractant, the method achieved better sensitivity and enrichment factor. Matrix effects were evaluated. Developed methods were utilized in analysis of real samples originated from field trial and markets. For field trial, pre-harvest interval (PHI) and dissipation kinetics were investigated. QuEChERS-DLLME method detected ethion in all market samples and bifenthrin in two of them.
    Keywords bifenthrin ; chlorobenzene ; ethion ; field experimentation ; gas chromatography-mass spectrometry ; liquid-phase microextraction ; markets
    Language English
    Dates of publication 2018-12
    Size p. 3542-3550.
    Publishing place Springer US
    Document type Article
    ZDB-ID 2429656-9
    ISSN 1936-976X ; 1936-9751
    ISSN (online) 1936-976X
    ISSN 1936-9751
    DOI 10.1007/s12161-018-1333-8
    Database NAL-Catalogue (AGRICOLA)

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