LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 8 of total 8

Search options

  1. Article ; Online: Effects of D128N Mutation on OsSERK2 in Xa21-Mediated Immune Complex

    Raghib Ishraq Alvy / M. H. M. Mubassir / Mohd Firdaus Abdul-Wahab / Salehhuddin Hamdan

    Advances in Agriculture, Vol

    An In Silico Study

    2023  Volume 2023

    Abstract: Receptor-like kinases (RLKs) are plant proteins that form signaling circuits to transduce information through the plant cell membrane to the nucleus and activate processes that direct growth, development, stress response, and disease resistance. Upon ... ...

    Abstract Receptor-like kinases (RLKs) are plant proteins that form signaling circuits to transduce information through the plant cell membrane to the nucleus and activate processes that direct growth, development, stress response, and disease resistance. Upon sensing various environmental stress stimuli, RLKs interact with specific targets and recruit several other proteins to initiate the defense mechanism. Among many RLK subfamilies, leucine-rich repeat RLKs (LRR-RLKs) are the largest. Xa21, a member of LRR-RLK, is a vital receptor protein in rice plants that binds with bacterial RaxX21-sY, whereas OsSERK2 is a somatic embryogenic receptor kinase (SERK) that acts as a coreceptor in this process. This study focuses on the effect of a substitution mutation of aspartate128 with asparagine128 (D128N) in OsSERK2 on the interdependent binding pattern of the Xa21, RaxX21-sY, and OsSERK2 D128N proteins. The in silico results showed that the D128N mutation in OsSERK2 can significantly change the interaction pattern of the critical residues of the OsSERK2 and affects its receptor-ligand (Xa21-RaxX21-sY) interaction in the complex. These findings are expected to significantly contribute to the study of the structural basis of Xa21-mediated immunity and the first layer of plant defense mechanisms, thereby aiding further research on these structures and their phenotypic implications.
    Keywords Agriculture (General) ; S1-972
    Subject code 580
    Language English
    Publishing date 2023-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article ; Online: A Novel Protein Interaction between Nucleotide Binding Domain of Hsp70 and p53 Motif

    Asita Elengoe / Mohammed Abu Naser / Salehhuddin Hamdan

    International Journal of Genomics, Vol

    2015  Volume 2015

    Keywords Genetics ; QH426-470 ; Biology (General) ; QH301-705.5 ; Science ; Q
    Language English
    Publishing date 2015-01-01T00:00:00Z
    Publisher Hindawi Publishing Corporation
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article: Molecular dynamics simulation and docking studies on novel mutants (T11V, T12P and D364S) of the nucleotide-binding domain of human heat shock 70 kDa protein

    Elengoe, Asita / Mohammed Abu Naser / Salehhuddin Hamdan

    Biologia. 2016 Jan. 23, v. 70, no. 12

    2016  

    Abstract: The aim of investigating protein interaction between Homo sapiens adenovirus and heat shock 70 kDa protein (Hsp70) is to study a potentially synergistic interaction that would enhance the anti-apoptotic mechanisms, hence increasing the virus replication ... ...

    Abstract The aim of investigating protein interaction between Homo sapiens adenovirus and heat shock 70 kDa protein (Hsp70) is to study a potentially synergistic interaction that would enhance the anti-apoptotic mechanisms, hence increasing the virus replication rate and improve the killing efficiency of tumour cells in cancer therapy. Currently, the protein interaction between Hsp70 and E1A 32 kDa of human adenovirus C serotype 5 (Ad5) is still unknown. Mutant models (T11V, T12P and D364S) were built, simulated and their interactions with Ad5 were studied. The E1A 32 kDa of human Ad5 motif (PNLVP) showed the lowest binding energy and intermolecular energy values with the novel T11V mutant at -8.26 kcal/mol and -11.21 kcal/mol. The protein-ligand complex models revealed that the T11V mutant had the strongest and most stable interaction with the PNLVP motif among all the four protein-ligand complex structures. This knowledge would assist future in vivo investigations of this protein-ligand complex structure in cancer treatment research.
    Keywords Homo sapiens ; Human adenovirus C ; energy ; humans ; models ; molecular dynamics ; mutants ; neoplasm cells ; neoplasms ; protein-protein interactions ; serotypes ; therapeutics ; virus replication ; NBD of Hsp70 ; adenovirus serotype 5 ; PNLVP motif ; molecular dynamics simulation ; docking
    Language English
    Dates of publication 2016-0123
    Size p. 1655-1671.
    Publishing place De Gruyter Open
    Document type Article
    ZDB-ID 419136-5
    ISSN 1336-9563 ; 0006-3088 ; 1335-6372 ; 1335-6380
    ISSN (online) 1336-9563
    ISSN 0006-3088 ; 1335-6372 ; 1335-6380
    DOI 10.1515/biolog-2015-0194
    Database NAL-Catalogue (AGRICOLA)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 791: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Modeling and Docking Studies on Novel Mutants (K71L and T204V) of the ATPase Domain of Human Heat Shock 70 kDa Protein 1

    Asita Elengoe / Mohammed Abu Naser / Salehhuddin Hamdan

    International Journal of Molecular Sciences, Vol 15, Iss 4, Pp 6797-

    2014  Volume 6814

    Abstract: The purpose of exploring protein interactions between human adenovirus and heat shock protein 70 is to exploit a potentially synergistic interaction to enhance anti-tumoral efficacy and decrease toxicity in cancer treatment. However, the protein ... ...

    Abstract The purpose of exploring protein interactions between human adenovirus and heat shock protein 70 is to exploit a potentially synergistic interaction to enhance anti-tumoral efficacy and decrease toxicity in cancer treatment. However, the protein interaction of Hsp70 with E1A32 kDa of human adenovirus serotype 5 remains to be elucidated. In this study, two residues of ATPase domain of human heat shock 70 kDa protein 1 (PDB: 1 HJO) were mutated. 3D mutant models (K71L and T204V) using PyMol software were then constructed. The structures were evaluated by PROCHECK, ProQ, ERRAT, Verify 3D and ProSA modules. All evidence suggests that all protein models are acceptable and of good quality. The E1A32 kDa motif was retrieved from UniProt (P03255), as well as subjected to docking interaction with NBD, K71L and T204V, using the Autodock 4.2 program. The best lowest binding energy value of −9.09 kcal/mol was selected for novel T204V. Moreover, the protein-ligand complex structures were validated by RMSD, RMSF, hydrogen bonds and salt bridge analysis. This revealed that the T204V-E1A32 kDa motif complex was the most stable among all three complex structures. This study provides information about the interaction between Hsp70 and the E1A32 kDa motif, which emphasizes future perspectives to design rational drugs and vaccines in cancer therapy.
    Keywords adenovirus ; Hsp70 ; PROCHECK ; ProQ ; ERRAT ; Verify 3D ; ProSA ; docking ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 572
    Language English
    Publishing date 2014-04-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article ; Online: The Effects of Zeolite X and Y on Cancer Cell Lines

    Noor Azhana Ghazi / Khairina ‘Izzati Amir Hussain / Nik Ahmad Nizam Nik Malek / Salehhuddin Hamdan

    Journal of Science and Technology, Vol 4, Iss

    2012  Volume 1

    Abstract: Zeolites are hydrated silicates of aluminium that have been very useful in many industry because of its microporous property, absorbance ability and ion exchange capacity. It is currently viewed as a potential adjuvant in cancer therapy due to its ... ...

    Abstract Zeolites are hydrated silicates of aluminium that have been very useful in many industry because of its microporous property, absorbance ability and ion exchange capacity. It is currently viewed as a potential adjuvant in cancer therapy due to its ability to inhibit the proliferation of cancer cells. Research on natural zeolite clinoptilolite application as anticancer agent has been proven by others. However, the effect of other types of zeolite on cancer cells is still uncertain. This study is performed to determine the effects of zeolite X and Y on cancer cell lines proliferation in vitro. Cancer cell lines HeLa, AsPC-1 and 911 cells were cultured in designated medium treated with zeolite X and zeolite Y at the concentration of 5 mg/ml and 50 mg/ml. Fetal Bovine Serum (FBS) concentrations were modified to 5%, 10%, 15% and 20%. After 72 hours incubation, the efficacy of zeolite to treat cancer cell lines were measured by means of cell viability test via MTT assay. Overall results showed that cancer cell lines cultivated in the medium treated with 50 mg/ml of zeolite X and 5% FBS exhibited the highest inhibition of cell proliferation and decrease in cell viability. This finding provides preliminary information in the study of determining the potential use of zeolite as anticancer agent for alternative or complementary therapy.
    Keywords Zeolite X ; zeolite Y ; anticancer agent ; cancer therapy ; complementary therapy ; Science (General) ; Q1-390 ; Science ; Q
    Subject code 610
    Language English
    Publishing date 2012-07-01T00:00:00Z
    Publisher Penerbit UTHM
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Article ; Online: Pharmacological Mechanisms Underlying Gastroprotective Activities of the Fractions Obtained from Polygonum minus in Sprague Dawley Rats

    Salehhuddin Hamdan / Hasnah Mohd Sirat / Suhailah Wasman Qader / Lee Suan Chua / Mahmood Ameen Abdulla

    International Journal of Molecular Sciences, Vol 13, Iss 2, Pp 1481-

    2012  Volume 1496

    Abstract: The leaves of Polygonum minus were fractionated using an eluting solvent to evaluate the pharmacological mechanisms underlying the anti-ulcerogenic activity of P. minus. Different P. minus fractions were obtained and evaluated for their ulcer preventing ... ...

    Abstract The leaves of Polygonum minus were fractionated using an eluting solvent to evaluate the pharmacological mechanisms underlying the anti-ulcerogenic activity of P. minus. Different P. minus fractions were obtained and evaluated for their ulcer preventing capabilities using the ethanol induction method. In this study, Sprague Dawley rats weighing 150–200 g were used. Different parameters were estimated to identify the active fraction underlying the mechanism of the gastroprotective action of P. minus: the gastric mucus barrier, as well as superoxide dismutase, total hexosamine, and prostaglandin synthesis. Amongst the five fractions from the ethanolic extract of P. minus, the ethyl acetate:methanol 1:1 v/v fraction (F2) significantly (p < 0.005) exhibited better inhibition of ulcer lesions in a dose-dependent manner. In addition, rats pre-treated with F2 showed a significant elevation in superoxide dismutase (SOD), hexosamine and PGE2 levels in the stomach wall mucosa in a dose-dependent matter. Based on these results, the ethyl acetate:methanol 1:1 v/v fraction was considered to be the best fraction for mucous protection in the ethanol induction model. The mechanisms underlying this protection were attributed to the synthesis of antioxidants and PGE2.
    Keywords Polygonum minus ; HPLC ; UPLC-ESI-MS/MS ; gastroprotective mechanisms ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 540
    Language English
    Publishing date 2012-02-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  7. Article ; Online: Antioxidant, Total Phenolic Content and Cytotoxicity Evaluation of Selected Malaysian Plants

    Nigar Najim / Salehhuddin Hamdan / Mazatulikhma Mat Zain / Lee Suan Chua / Mahmood Ameen Abdulla / Suhailah Wasman Qader

    Molecules, Vol 16, Iss 4, Pp 3433-

    2011  Volume 3443

    Abstract: Aqueous and ethanol extracts of different traditional Malaysian plants (Polygonum minus, Andrographis paniculata, Curcuma xanthorrhiza, Momordica charantia and Strobilanthes crispus) were evaluated for their antioxidant properties, total phenolic content ...

    Abstract Aqueous and ethanol extracts of different traditional Malaysian plants (Polygonum minus, Andrographis paniculata, Curcuma xanthorrhiza, Momordica charantia and Strobilanthes crispus) were evaluated for their antioxidant properties, total phenolic content and cytotoxic activity. Antioxidant activity was evaluated by using 1,1-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. The results showed that ethanol extracts contain high antioxidant activities compared to aqueous extracts. The findings exhibited a strong correlation between antioxidant activity and the total phenol contents. In addition, all the plant extracts showed non-toxic effects against a normal human lung fibroblast cell line (Hs888Lu). Although traditionally aqueous extracts are used, we determined that ethanol extracts usually achieved better activity in the assays.
    Keywords antioxidant activity ; total phenolic content ; cytotoxicity ; Malaysian plants ; Organic chemistry ; QD241-441
    Language English
    Publishing date 2011-04-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  8. Article: Molecular dynamics simulation and docking studies on novel mutants (T11V, T12P and D364S) of the nucleotide-binding domain of human heat shock 70 kDa protein

    Elengoe, Asita / Mohammed Abu Naser / Salehhuddin Hamdan

    Biologia

    Volume v. 70,, Issue no. 1

    Abstract: The aim of investigating protein interaction between Homo sapiens adenovirus and heat shock 70 kDa protein (Hsp70) is to study a potentially synergistic interaction that would enhance the anti-apoptotic mechanisms, hence increasing the virus replication ... ...

    Abstract The aim of investigating protein interaction between Homo sapiens adenovirus and heat shock 70 kDa protein (Hsp70) is to study a potentially synergistic interaction that would enhance the anti-apoptotic mechanisms, hence increasing the virus replication rate and improve the killing efficiency of tumour cells in cancer therapy. Currently, the protein interaction between Hsp70 and E1A 32 kDa of human adenovirus C serotype 5 (Ad5) is still unknown. Mutant models (T11V, T12P and D364S) were built, simulated and their interactions with Ad5 were studied. The E1A 32 kDa of human Ad5 motif (PNLVP) showed the lowest binding energy and intermolecular energy values with the novel T11V mutant at -8.26 kcal/mol and -11.21 kcal/mol. The protein-ligand complex models revealed that the T11V mutant had the strongest and most stable interaction with the PNLVP motif among all the four protein-ligand complex structures. This knowledge would assist future in vivo investigations of this protein-ligand complex structure in cancer treatment research.
    Language English
    Document type Article
    ISSN 1336-9563
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top