LIVIVO - Suchergebnisse -

Suchergebnis

Treffer 1 - 10 von insgesamt 15

Suchoptionen

Artikel ; Online: In Vivo and Ex Vivo View of Newt Lens Regeneration.

Tsissios, Georgios / Sallese, Anthony / Chen, Weihao / Miller, Alyssa / Wang, Hui / Del Rio-Tsonis, Katia

Methods in molecular biology (Clifton, N.J.)

2022 Band 2562, Seite(n) 197–208

Abstract: Lens regeneration in the adult newt illustrates a unique example of naturally occurring cell transdifferentiation. During this process, iris pigmented epithelial cells (iPECs) reprogram into a lens, a tissue that is derived from a different embryonic ... ...

Abstract	Lens regeneration in the adult newt illustrates a unique example of naturally occurring cell transdifferentiation. During this process, iris pigmented epithelial cells (iPECs) reprogram into a lens, a tissue that is derived from a different embryonic source. Several methodologies both in vivo and in culture have been utilized over the years to observe this phenomenon. Most recently, Optical Coherence Tomography (OCT) has been identified as an effective tool to study the lens regeneration process in continuity through noninvasive, real-time imaging of the same animal. Described in this chapter are three different methodologies that can be used to observe the newt lens regeneration process both in vivo and ex vivo.
Mesh-Begriff(e)	Animals ; Lens, Crystalline ; Salamandridae ; Cell Transdifferentiation ; Epithelial Cells
Sprache	Englisch
Erscheinungsdatum	2022-10-21
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-2659-7_13
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

Fernleihe an ZB MED

Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Details ▾
- Kostenpflichtig bestellen

Artikel ; Online: Isolation and Characterization of Peritoneal Macrophages from Salamanders.

Sallese, Anthony / Tsissios, Georgios / Pérez-Estrada, J Raúl / Martinez, Arielle / Del Rio-Tsonis, Katia

Methods in molecular biology (Clifton, N.J.)

2022 Band 2562, Seite(n) 259–270

Abstract: Salamanders have been used as research models for centuries. While they exhibit a wide range of biological features not seen in mammals, none has captivated scientists like their ability to regenerate. Interestingly, axolotl macrophages have emerged as ... ...

Abstract	Salamanders have been used as research models for centuries. While they exhibit a wide range of biological features not seen in mammals, none has captivated scientists like their ability to regenerate. Interestingly, axolotl macrophages have emerged as an essential cell population for tissue regeneration. Whether the same is true in other salamanders such as newt species Notophthalmus viridescens, Cynops pyrrhogaster, or Pleurodeles waltl remains to be seen. Unfortunately, regardless of the species, molecular tools to study macrophage function in salamanders are lacking. We propose that the readily available, terminally differentiated peritoneal macrophages from newts or axolotls could be used to validate molecular reagents in the study of macrophage function during tissue regeneration in salamanders.
Mesh-Begriff(e)	Animals ; Urodela ; Macrophages, Peritoneal ; Pleurodeles ; Mammals ; Salamandridae
Sprache	Englisch
Erscheinungsdatum	2022-10-19
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-2659-7_18
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Fernleihe an ZB MED

Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Details ▾
- Kostenpflichtig bestellen

Artikel ; Online: Mass spectrometry based metabolomics: a novel analytical technique for detecting metabolic syndrome?

Sallese, Anthony / Zhu, Jiangjiang

Bioanalysis

2017 Band 9, Heft 21, Seite(n) 1623–1626

Mesh-Begriff(e)	Biomarkers/analysis ; Humans ; Mass Spectrometry ; Metabolic Syndrome/diagnosis ; Metabolic Syndrome/metabolism ; Metabolomics
Chemische Substanzen	Biomarkers
Sprache	Englisch
Erscheinungsdatum	2017-11-02
Erscheinungsland	England
Dokumenttyp	Editorial
ISSN	1757-6199
ISSN (online)	1757-6199
DOI	10.4155/bio-2017-0165
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Fernleihe an ZB MED

Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Details ▾
- Kostenpflichtig bestellen

Artikel ; Online: Macrophages modulate fibrosis during newt lens regeneration.

Tsissios, Georgios / Sallese, Anthony / Perez-Estrada, J Raul / Tangeman, Jared A / Chen, Weihao / Smucker, Byran / Ratvasky, Sophia C / Grajales-Esquivel, Erika / Martinez, Arielle / Visser, Kimberly J / Joven Araus, Alberto / Wang, Hui / Simon, András / Yun, Maximina H / Del Rio-Tsonis, Katia

Stem cell research & therapy

2024 Band 15, Heft 1, Seite(n) 141

Abstract: Background: Previous studies have suggested that macrophages are present during lens regeneration in newts, but their role in the process is yet to be elucidated.: Methods: Here we generated a transgenic reporter line using the newt, Pleurodeles ... ...

Abstract	Background: Previous studies have suggested that macrophages are present during lens regeneration in newts, but their role in the process is yet to be elucidated. Methods: Here we generated a transgenic reporter line using the newt, Pleurodeles waltl, that traces macrophages during lens regeneration. Furthermore, we assessed early changes in gene expression during lens regeneration using two newt species, Notophthalmus viridescens and Pleurodeles waltl. Finally, we used clodronate liposomes to deplete macrophages during lens regeneration in both species and tested the effect of a subsequent secondary injury after macrophage recovery. Results: Macrophage depletion abrogated lens regeneration, induced the formation of scar-like tissue, led to inflammation, decreased iris pigment epithelial cell (iPEC) proliferation, and increased rates of apoptosis in the eye. Some of these phenotypes persisted throughout the last observation period of 100 days and could be attenuated by exogenous FGF2 administration. A distinct transcript profile encoding acute inflammatory effectors was established for the dorsal iris. Reinjury of the newt eye alleviated the effects of macrophage depletion, including the resolution of scar-like tissue, and re-initiated the regeneration process. Conclusions: Together, our findings highlight the importance of macrophages for facilitating a pro-regenerative environment in the newt eye by regulating fibrotic responses, modulating the overall inflammatory landscape, and maintaining the proper balance of early proliferation and late apoptosis of the iPECs.
Mesh-Begriff(e)	Animals ; Macrophages/metabolism ; Regeneration/drug effects ; Fibrosis ; Lens, Crystalline/metabolism ; Lens, Crystalline/cytology ; Lens, Crystalline/injuries ; Salamandridae ; Apoptosis/drug effects ; Cell Proliferation/drug effects
Sprache	Englisch
Erscheinungsdatum	2024-05-14
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	2548671-8
ISSN	1757-6512 ; 1757-6512
ISSN (online)	1757-6512
ISSN	1757-6512
DOI	10.1186/s13287-024-03740-1
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Details ▾
- Kostenpflichtig bestellen

Artikel ; Online: Characterizing the lens regeneration process in Pleurodeles waltl.

Tsissios, Georgios / Theodoroudis-Rapp, Gabriella / Chen, Weihao / Sallese, Anthony / Smucker, Byran / Ernst, Lake / Chen, Junfan / Xu, Yiqi / Ratvasky, Sophia / Wang, Hui / Del Rio-Tsonis, Katia

Differentiation; research in biological diversity

2023 Band 132, Seite(n) 15–23

Abstract: Background: Aging and regeneration are heavily linked processes. While it is generally accepted that regenerative capacity declines with age, some vertebrates, such as newts, can bypass the deleterious effects of aging and successfully regenerate a lens ...

Abstract	Background: Aging and regeneration are heavily linked processes. While it is generally accepted that regenerative capacity declines with age, some vertebrates, such as newts, can bypass the deleterious effects of aging and successfully regenerate a lens throughout their lifetime. Results: Here, we used Spectral-Domain Optical Coherence Tomography (SD-OCT) to monitor the lens regeneration process of larvae, juvenile, and adult newts. While all three life stages were able to regenerate a lens through transdifferentiation of the dorsal iris pigment epithelial cells (iPECs), an age-related change in the kinetics of the regeneration process was observed. Consistent with these findings, iPECs from older animals exhibited a delay in cell cycle re-entry. Furthermore, it was observed that clearance of the extracellular matrix (ECM) was delayed in older organisms. Conclusions: Collectively, our results suggest that although lens regeneration capacity does not decline throughout the lifespan of newts, the intrinsic and extrinsic cellular changes associated with aging alter the kinetics of this process. By understanding how these changes affect lens regeneration in newts, we can gain important insights for restoring the age-related regeneration decline observed in most vertebrates.
Mesh-Begriff(e)	Animals ; Pleurodeles ; Salamandridae ; Lens, Crystalline ; Extracellular Matrix ; Cell Division
Sprache	Englisch
Erscheinungsdatum	2023-03-11
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	184540-8
ISSN	1432-0436 ; 0301-4681
ISSN (online)	1432-0436
ISSN	0301-4681
DOI	10.1016/j.diff.2023.02.003
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Volltext online

Zugriff für angemeldete ZB MED Nutzerinnen und Nutzer

Zusatzmaterialien

Verfügbar in ZB MED Köln/Königswinter

Zs.A 1170: Hefte anzeigen

Standort:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Über subito bestellen

Details ▾

Artikel ; Online: In Vivo Imaging of Newt Lens Regeneration: Novel Insights Into the Regeneration Process.

Chen, Weihao / Tsissios, Georgios / Sallese, Anthony / Smucker, Byran / Nguyen, Anh-Thu / Chen, Junfan / Wang, Hui / Del Rio-Tsonis, Katia

Translational vision science & technology

2021 Band 10, Heft 10, Seite(n) 4

Abstract: Purpose: To establish optical coherence tomography (OCT) as an in vivo imaging modality for investigating the process of newt lens regeneration.: Methods: Spectral-domain OCT was employed for in vivo imaging of the newt lens regeneration process. A ... ...

Abstract	Purpose: To establish optical coherence tomography (OCT) as an in vivo imaging modality for investigating the process of newt lens regeneration. Methods: Spectral-domain OCT was employed for in vivo imaging of the newt lens regeneration process. A total of 37 newts were lentectomized and followed by OCT imaging over the course of 60 to 80 days. Histological images were obtained at several time points to compare with the corresponding OCT images. Volume measurements were also acquired. Results: OCT can identify the key features observed in corresponding histological images based on the scattering differences from various eye tissues, such as the cornea, intact and regenerated lens, and the iris. Lens volume measurements from three-dimensional OCT images showed that the regenerating lens size increased linearly until 60 days post-lentectomy. Conclusions: Using OCT imaging, we were able to track the entire process of newt lens regeneration in vivo for the first time. Three-dimensional OCT images allowed us to volumetrically quantify and visualize the dynamic spatial relationships between tissues during the regeneration process. Our results establish OCT as an in vivo imaging modality to track/analyze the entire lens regeneration process from the same animal. Translational relevance: Lens regeneration in newts represents a unique example of vertebrate tissue plasticity. Investigating the cellular and morphological events that govern this extraordinary process in vivo will advance our understanding and shed light on developing new therapies to treat blinding disorders in higher vertebrates.
Mesh-Begriff(e)	Animals ; Iris ; Lens, Crystalline/diagnostic imaging ; Regeneration ; Salamandridae ; Tomography, Optical Coherence
Sprache	Englisch
Erscheinungsdatum	2021-08-12
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2674602-5
ISSN	2164-2591 ; 2164-2591
ISSN (online)	2164-2591
ISSN	2164-2591
DOI	10.1167/tvst.10.10.4
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Details ▾
- Kostenpflichtig bestellen

Artikel: Macrophages modulate fibrosis during newt lens regeneration.

Tsissios, Georgios / Sallese, Anthony / Perez-Estrada, J Raul / Tangeman, Jared A / Chen, Weihao / Smucker, Byran / Ratvasky, Sophia C / Grajales-Esquivel, Erika / Martinez, Arielle / Visser, Kimberly J / Araus, Alberto Joven / Wang, Hui / Simon, Andras / Yun, Maximina H / Rio-Tsonis, Katia Del

bioRxiv : the preprint server for biology

2023

Abstract: Previous studies indicated that macrophages play a role during lens regeneration in newts, but their function has not been tested experimentally. Here we generated a transgenic newt reporter line in which macrophages can be ... ...

Abstract	Previous studies indicated that macrophages play a role during lens regeneration in newts, but their function has not been tested experimentally. Here we generated a transgenic newt reporter line in which macrophages can be visualized
Sprache	Englisch
Erscheinungsdatum	2023-06-05
Erscheinungsland	United States
Dokumenttyp	Preprint
DOI	10.1101/2023.06.04.543633
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Fernleihe an ZB MED

Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Details ▾

Artikel: Macrophages modulate fibrosis during newt lens regeneration.

Tsissios, Georgios / Sallese, Anthony / Perez-Estrada, J Raul / Tangeman, Jared A / Chen, Weihao / Smucker, Byran / Ratvasky, Sophia C / Grajales-Esquive, Erika L / Martinez, Arielle / Visser, Kimberly J / Araus, Alberto Joven / Wang, Hui / Simon, Andras / Yun, Maximina H / Rio-Tsonis, Katia Del

Research square

2023

Abstract: Background: Previous studies indicated that macrophages play a role during lens regeneration in newts, but their function has not been tested experimentally.: Methods: Here we generated a transgenic newt reporter line in which macrophages can be ... ...

Abstract	Background: Previous studies indicated that macrophages play a role during lens regeneration in newts, but their function has not been tested experimentally. Methods: Here we generated a transgenic newt reporter line in which macrophages can be visualized Results: Macrophage depletion induced the formation of scar-like tissue, an increased and sustained inflammatory response, an early decrease in iris pigment epithelial cell (iPEC) proliferation and a late increase in apoptosis. Some of these phenotypes persisted for at least 100 days and could be rescued by exogenous FGF2. Re-injury alleviated the effects of macrophage depletion and re-started the regeneration process. Conclusions: Together, our findings highlight the importance of macrophages in facilitating a pro-regenerative environment in the newt eye, helping to resolve fibrosis, modulating the overall inflammatory landscape and maintaining the proper balance of early proliferation and late apoptosis.
Sprache	Englisch
Erscheinungsdatum	2023-11-25
Erscheinungsland	United States
Dokumenttyp	Preprint
DOI	10.21203/rs.3.rs-3603645/v1
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Fernleihe an ZB MED

Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Details ▾

Artikel ; Online: A murine model of hereditary pulmonary alveolar proteinosis caused by homozygous

Shima, Kenjiro / Arumugam, Paritha / Sallese, Anthony / Horio, Yuko / Ma, Yan / Trapnell, Cole / Wessendarp, Matthew / Chalk, Claudia / McCarthy, Cormac / Carey, Brenna C / Trapnell, Bruce C / Suzuki, Takuji

American journal of physiology. Lung cellular and molecular physiology

2022 Band 322, Heft 3, Seite(n) L438–L448

Abstract: Hereditary pulmonary alveolar proteinosis (hPAP) is a rare disorder caused by recessive mutations in GM-CSF receptor subunit α/β genes ( ...

Abstract	Hereditary pulmonary alveolar proteinosis (hPAP) is a rare disorder caused by recessive mutations in GM-CSF receptor subunit α/β genes (
Mesh-Begriff(e)	Animals ; Disease Models, Animal ; Granulocyte-Macrophage Colony-Stimulating Factor/genetics ; Humans ; Macrophages, Alveolar/metabolism ; Mice ; Pulmonary Alveolar Proteinosis/genetics ; Pulmonary Alveolar Proteinosis/metabolism ; Pulmonary Surfactants/metabolism ; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor/genetics ; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor/metabolism ; Surface-Active Agents/metabolism
Chemische Substanzen	CSF2RA protein, human ; Csf2ra protein, mouse ; Pulmonary Surfactants ; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor ; Surface-Active Agents ; Granulocyte-Macrophage Colony-Stimulating Factor (83869-56-1)
Sprache	Englisch
Erscheinungsdatum	2022-01-19
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1013184-x
ISSN	1522-1504 ; 1040-0605
ISSN (online)	1522-1504
ISSN	1040-0605
DOI	10.1152/ajplung.00175.2021
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Verfügbar in ZB MED Köln/Königswinter

Uc I Zs.89: Hefte anzeigen			Standort: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.A 2749: Hefte anzeigen			Standort: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)

Über subito bestellen

Details ▾
- Im ZB MED-Bestand
- Kostenpflichtig bestellen

Artikel ; Online: Long-Term Safety and Efficacy of Gene-Pulmonary Macrophage Transplantation Therapy of PAP in Csf2ra

Arumugam, Paritha / Suzuki, Takuji / Shima, Kenjiro / McCarthy, Cormac / Sallese, Anthony / Wessendarp, Matthew / Ma, Yan / Meyer, Johann / Black, Diane / Chalk, Claudia / Carey, Brenna / Lachmann, Nico / Moritz, Thomas / Trapnell, Bruce C

Molecular therapy : the journal of the American Society of Gene Therapy

2019 Band 27, Heft 9, Seite(n) 1597–1611

Abstract: Hereditary pulmonary alveolar proteinosis (PAP) is a genetic lung disease characterized by surfactant accumulation and respiratory failure arising from disruption of GM-CSF signaling. While mutations in either CSF2RA or CSF2RB (encoding GM-CSF receptor α ...

Abstract	Hereditary pulmonary alveolar proteinosis (PAP) is a genetic lung disease characterized by surfactant accumulation and respiratory failure arising from disruption of GM-CSF signaling. While mutations in either CSF2RA or CSF2RB (encoding GM-CSF receptor α or β chains, respectively) can cause PAP, α chain mutations are responsible in most patients. Pulmonary macrophage transplantation (PMT) is a promising new cell therapy in development; however, no studies have evaluated this approach for hereditary PAP (hPAP) caused by Csf2ra mutations. Here, we report on the preclinical safety, tolerability, and efficacy of lentiviral-vector (LV)-mediated Csf2ra expression in macrophages and PMT of gene-corrected macrophages (gene-PMT therapy) in Csf2ra gene-ablated (Csf2ra
Mesh-Begriff(e)	Animals ; Cell Proliferation ; Cell- and Tissue-Based Therapy/methods ; Disease Models, Animal ; Gene Expression ; Genetic Therapy/methods ; Genetic Vectors/genetics ; Immunophenotyping ; Lentivirus/genetics ; Macrophages, Alveolar/metabolism ; Macrophages, Alveolar/transplantation ; Mice ; Mice, Knockout ; Pulmonary Alveolar Proteinosis/diagnosis ; Pulmonary Alveolar Proteinosis/genetics ; Pulmonary Alveolar Proteinosis/therapy ; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor/genetics ; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor/metabolism ; Signal Transduction ; Transduction, Genetic
Chemische Substanzen	Csf2ra protein, mouse ; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
Sprache	Englisch
Erscheinungsdatum	2019-07-02
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2010592-7
ISSN	1525-0024 ; 1525-0016
ISSN (online)	1525-0024
ISSN	1525-0016
DOI	10.1016/j.ymthe.2019.06.010
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Volltext online

Zugriff für angemeldete ZB MED Nutzerinnen und Nutzer

Zusatzmaterialien

Verfügbar in ZB MED Köln/Königswinter

Zs.A 5640: Hefte anzeigen

Standort:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Über subito bestellen

Details ▾

Zum Seitenanfang

Ihre letzten Suchen

Zusatzmaterialien

Kategorien

Über subito bestellen

Fernleihe an ZB MED

Zusatzmaterialien

Kategorien

Über subito bestellen

Fernleihe an ZB MED

Zusatzmaterialien

Kategorien

Über subito bestellen

Fernleihe an ZB MED

Zusatzmaterialien

Kategorien

Über subito bestellen

Volltext online

Zusatzmaterialien

Kategorien

Verfügbar in ZB MED Köln/Königswinter

Über subito bestellen

Zusatzmaterialien

Kategorien

Über subito bestellen

Zusatzmaterialien

Kategorien

Fernleihe an ZB MED

Zusatzmaterialien

Kategorien

Fernleihe an ZB MED

Zusatzmaterialien

Kategorien

Verfügbar in ZB MED Köln/Königswinter

Über subito bestellen

Volltext online

Zusatzmaterialien

Kategorien

Verfügbar in ZB MED Köln/Königswinter

Über subito bestellen