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Article: [CHANGE IN THE CONDITION OF LOWER LIMB VEINS IN COSMONAUTS ON LONG-DURATION MISSIONS].

Kotovskaya, A R / Fomina, G A / Salnikov, A V

Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine

2015 Volume 49, Issue 5, Page(s) 5–10

Abstract: The paper reports changes in the main parameters of the lower limb veins in 36 cosmonauts in the course of 6-month space missions. Major reduction in the leg volume was shown in all cosmonauts, as well as a significant increase of venous capacity and ... ...

Abstract	The paper reports changes in the main parameters of the lower limb veins in 36 cosmonauts in the course of 6-month space missions. Major reduction in the leg volume was shown in all cosmonauts, as well as a significant increase of venous capacity and compliance. Rate of vein filling exhibited different trends, i.e. decreased in the majority of cosmonauts (n = 26, 74%) and increased in the rest (n = 9, 26%). Increases in venous capacity, compliance and filling rate may impact orthostatic stability (OS). These changes are among the pathophysiological mechanisms of the OS loss in space flight.
MeSH term(s)	Adult ; Astronauts ; Elasticity ; Follow-Up Studies ; Hemodynamics/physiology ; Humans ; Lower Extremity/blood supply ; Male ; Middle Aged ; Posture/physiology ; Rest ; Space Flight ; Time Factors ; Veins/physiology
Language	Russian
Publishing date	2015
Publishing country	Russia (Federation)
Document type	English Abstract ; Journal Article
ZDB-ID	1147752-0
ISSN	0233-528X ; 0321-5040
ISSN	0233-528X ; 0321-5040
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Article: [Normal values of the major parameters of lower limb veins in Russian cosmonauts prior to flight and in healthy untrained subjects].

Kotovskaya, A R / Fomina, G A / Salnikov, A V

Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine

2015 Volume 49, Issue 1, Page(s) 13–18

Abstract: The article presents normal values of the major parameters of lower limb veins in cosmonauts during preparations for space flight and volunteers leading a common life. The authors report the results and discuss the causes for differences in normal leg ... ...

Abstract	The article presents normal values of the major parameters of lower limb veins in cosmonauts during preparations for space flight and volunteers leading a common life. The authors report the results and discuss the causes for differences in normal leg venous parameters in these groups of subjects. Incomparability of measurements made in cosmonauts and common people is demonstrated. Changes in lower limb veins of a cosmonaut in microgravity can be evaluated only relative to his/her normal values of the major venous parameters (capacitance, compliance and filling) before flight.
MeSH term(s)	Adult ; Astronauts ; Humans ; Lower Extremity/blood supply ; Middle Aged ; Reference Values ; Russia ; Space Flight ; Weightlessness
Language	Russian
Publishing date	2015
Publishing country	Russia (Federation)
Document type	English Abstract ; Journal Article
ZDB-ID	1147752-0
ISSN	0233-528X ; 0321-5040
ISSN	0233-528X ; 0321-5040
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Article ; Online: ACKR4 Recruits GRK3 Prior to β-Arrestins but Can Scavenge Chemokines in the Absence of β-Arrestins.

Matti, Christoph / Salnikov, Angela / Artinger, Marc / D'Agostino, Gianluca / Kindinger, Ilona / Uguccioni, Mariagrazia / Thelen, Marcus / Legler, Daniel F

Frontiers in immunology

2020 Volume 11, Page(s) 720

Abstract: Chemokines are essential for guiding cell migration. Atypical chemokine receptors (ACKRs) contribute to the cell migration process by binding, internalizing and degrading local chemokines, which enables the formation of confined gradients. ACKRs are ... ...

Abstract	Chemokines are essential for guiding cell migration. Atypical chemokine receptors (ACKRs) contribute to the cell migration process by binding, internalizing and degrading local chemokines, which enables the formation of confined gradients. ACKRs are heptahelical membrane spanning molecules structurally related to G-protein coupled receptors (GPCRs), but seem to be unable to signal through G-proteins upon ligand binding. ACKR4 internalizes the chemokines CCL19, CCL21, and CCL25 and is best known for shaping functional CCL21 gradients. Ligand binding to ACKR4 has been shown to recruit β-arrestins that has led to the assumption that chemokine scavenging relies on β-arrestin-mediated ACKR4 trafficking, a common internalization route taken by class A GPCRs. Here, we show that CCL19, CCL21, and CCL25 readily recruited β-arrestin1 and β-arrestin2 to human ACKR4, but found no evidence for β-arrestin-dependent or independent ACKR4-mediated activation of the kinases Erk1/2, Akt, or Src. However, we demonstrate that β-arrestins interacted with ACKR4 in the steady-state and contributed to the spontaneous trafficking of the receptor in the absence of chemokines. Deleting the C-terminus of ACKR4 not only interfered with the interaction of β-arrestins, but also with the uptake of fluorescently labeled cognate chemokines. We identify the GPCR kinase GRK3, and to a lesser extent GRK2, but not GRK4, GRK5, and GRK6, to be recruited to chemokine-stimulated ACKR4. We show that GRK3 recruitment proceded the recruitment of β-arrestins upon ACKR4 engagement and that GRK2/3 inhibition partially interfered with steady-state interaction and chemokine-driven recruitment of β-arrestins to ACKR4. Overexpressing β-arrestin2 accelerated the uptake of fluorescently labeled CCL19, indicating that β-arrestins contribute to the chemokine scavenging activity of ACKR4. By contrast, cells lacking β-arrestins were still capable to take up fluorescently labeled CCL19 demonstrating that β-arrestins are dispensable for chemokine scavenging by ACKR4.
MeSH term(s)	Chemokine CCL19/metabolism ; Chemokine CCL21/metabolism ; Chemokines, CC/metabolism ; G-Protein-Coupled Receptor Kinase 3/metabolism ; HeLa Cells ; Humans ; Plasmids/genetics ; Plasmids/metabolism ; Protein Binding/genetics ; Receptors, CCR/genetics ; Receptors, CCR/metabolism ; Receptors, CCR7/genetics ; Receptors, CCR7/metabolism ; Signal Transduction/genetics ; Transfection ; beta-Arrestin 1/metabolism ; beta-Arrestin 2/genetics ; beta-Arrestin 2/metabolism
Chemical Substances	ACKR4 protein, human ; ARRB1 protein, human ; ARRB2 protein, human ; CCL19 protein, human ; CCL21 protein, human ; CCL25 protein, human ; CCR7 protein, human ; Chemokine CCL19 ; Chemokine CCL21 ; Chemokines, CC ; Receptors, CCR ; Receptors, CCR7 ; beta-Arrestin 1 ; beta-Arrestin 2 ; G-Protein-Coupled Receptor Kinase 3 (EC 2.7.11.15) ; GRK3 protein, human (EC 2.7.11.15)
Language	English
Publishing date	2020-04-22
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2020.00720
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Book ; Online: Data management and execution systems for the Rubin Observatory Science Pipelines

Lust, Nate B. / Jenness, Tim / Bosch, James F. / Salnikov, Andrei / Pease, Nathan M. / Gower, Michelle / Kowalik, Mikolaj / Dubois-Felsmann, Gregory P. / Mueller, Fritz / Schellart, Pim

2023

Abstract: We present the Rubin Observatory system for data storage/retrieval and pipelined code execution. The layer for data storage and retrieval is named the Butler. It consists of a relational database, known as the registry, to keep track of metadata and ... ...

Abstract	We present the Rubin Observatory system for data storage/retrieval and pipelined code execution. The layer for data storage and retrieval is named the Butler. It consists of a relational database, known as the registry, to keep track of metadata and relations, and a system to manage where the data is located, named the datastore. Together these systems create an abstraction layer that science algorithms can be written against. This abstraction layer manages the complexities of the large data volumes expected and allows algorithms to be written independently, yet be tied together automatically into a coherent processing pipeline. This system consists of tools which execute these pipelines by transforming them into execution graphs which contain concrete data stored in the Butler. The pipeline infrastructure is designed to be scalable in nature, allowing execution on environments ranging from a laptop all the way up to multi-facility data centers. This presentation will focus on the data management aspects as well as an overview on the creation of pipelines and the corresponding execution graphs. Comment: 4 pages, submitted to Astronomical Data Analysis Software and Systems XXXII, October 2022
Keywords	Astrophysics - Instrumentation and Methods for Astrophysics ; Computer Science - Distributed ; Parallel ; and Cluster Computing
Subject code	004
Publishing date	2023-03-06
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: CCL20 is a novel ligand for the scavenging atypical chemokine receptor 4.

Matti, Christoph / D'Uonnolo, Giulia / Artinger, Marc / Melgrati, Serena / Salnikov, Angela / Thelen, Sylvia / Purvanov, Vladimir / Strobel, Tobias D / Spannagel, Lisa / Thelen, Marcus / Legler, Daniel F

Journal of leukocyte biology

2020 Volume 107, Issue 6, Page(s) 1137–1154

Abstract: The chemokine CCL20 is broadly produced by endothelial cells in the liver, the lung, in lymph nodes and mucosal lymphoid tissues, and recruits CCR6 expressing leukocytes, particularly dendritic cells, mature B cells, and subpopulations of T cells. How ... ...

Abstract	The chemokine CCL20 is broadly produced by endothelial cells in the liver, the lung, in lymph nodes and mucosal lymphoid tissues, and recruits CCR6 expressing leukocytes, particularly dendritic cells, mature B cells, and subpopulations of T cells. How CCL20 is systemically scavenged is currently unknown. Here, we identify that fluorescently labeled human and mouse CCL20 are efficiently taken-up by the atypical chemokine receptor ACKR4. CCL20 shares ACKR4 with the homeostatic chemokines CCL19, CCL21, and CCL25, although with a lower affinity. We demonstrate that all 4 human chemokines recruit β-arrestin1 and β-arrestin2 to human ACKR4. Similarly, mouse CCL19, CCL21, and CCL25 equally activate the human receptor. Interestingly, at the same chemokine concentration, mouse CCL20 did not recruit β-arrestins to human ACKR4. Further cross-species analysis suggests that human ACKR4 preferentially takes-up human CCL20, whereas mouse ACKR4 similarly internalizes mouse and human CCL20. Furthermore, we engineered a fluorescently labeled chimeric chemokine consisting of the N-terminus of mouse CCL25 and the body of mouse CCL19, termed CCL25_19, which interacts with and is taken-up by human and mouse ACKR4.
MeSH term(s)	Amino Acid Sequence ; Animals ; B-Lymphocytes/cytology ; B-Lymphocytes/metabolism ; Binding Sites ; Cell Line ; Chemokine CCL19/chemistry ; Chemokine CCL19/genetics ; Chemokine CCL19/metabolism ; Chemokine CCL20/chemistry ; Chemokine CCL20/genetics ; Chemokine CCL20/metabolism ; Chemokine CCL21/chemistry ; Chemokine CCL21/genetics ; Chemokine CCL21/metabolism ; Chemokines, CC/chemistry ; Chemokines, CC/genetics ; Chemokines, CC/metabolism ; HEK293 Cells ; HeLa Cells ; Humans ; Ligands ; Mice ; Mutant Chimeric Proteins/chemistry ; Mutant Chimeric Proteins/genetics ; Mutant Chimeric Proteins/metabolism ; Protein Binding ; Protein Interaction Domains and Motifs ; Protein Isoforms/chemistry ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; Protein Structure, Secondary ; Receptors, CCR/chemistry ; Receptors, CCR/genetics ; Receptors, CCR/metabolism ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Sequence Alignment ; Sequence Homology, Amino Acid ; Species Specificity ; Transfection ; beta-Arrestins/genetics ; beta-Arrestins/metabolism
Chemical Substances	ACKR4 protein, human ; CCL19 protein, human ; CCL20 protein, human ; CCL21 protein, human ; CCL25 protein, human ; Chemokine CCL19 ; Chemokine CCL20 ; Chemokine CCL21 ; Chemokines, CC ; Ligands ; Mutant Chimeric Proteins ; Protein Isoforms ; Receptors, CCR ; Recombinant Proteins ; beta-Arrestins
Language	English
Publishing date	2020-06-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605722-6
ISSN	1938-3673 ; 0741-5400
ISSN (online)	1938-3673
ISSN	0741-5400
DOI	10.1002/JLB.2MA0420-295RRR
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Article: Data systems for the Linac coherent light source.

Thayer, J / Damiani, D / Ford, C / Dubrovin, M / Gaponenko, I / O'Grady, C P / Kroeger, W / Pines, J / Lane, T J / Salnikov, A / Schneider, D / Tookey, T / Weaver, M / Yoon, C H / Perazzo, A

Advanced structural and chemical imaging

2017 Volume 3, Issue 1, Page(s) 3

Abstract: The data systems for X-ray free-electron laser (FEL) experiments at the Linac coherent light source (LCLS) are described. These systems are designed to acquire and to reliably transport shot-by-shot data at a peak throughput of 5 GB/s to the offline data ...

Abstract	The data systems for X-ray free-electron laser (FEL) experiments at the Linac coherent light source (LCLS) are described. These systems are designed to acquire and to reliably transport shot-by-shot data at a peak throughput of 5 GB/s to the offline data storage where experimental data and the relevant metadata are archived and made available for user analysis. The analysis and monitoring implementation (AMI) and Photon Science ANAlysis (psana) software packages are described. Psana is open source and freely available.
Language	English
Publishing date	2017-01-14
Publishing country	Germany
Document type	Journal Article
ISSN	2198-0926
ISSN	2198-0926
DOI	10.1186/s40679-016-0037-7
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Book ; Online: Building a Distributed Computing System for LDMX

Bryngemark, Lene Kristian / Cameron, David / Dutta, Valentina / Eichlersmith, Thomas / Konya, Balazs / Moreno, Omar / Mullier, Geoffrey / Paganelli, Florido / Pöttgen, Ruth / Rogers, Fuzzy / Salnikov, Andrii / Weakliem, Paul

Challenges of creating and operating a lightweight e-infrastructure for small-to-medium size accelerator experiments

2021

Abstract: Particle physics experiments rely extensively on computing and data services, making e-infrastructure an integral part of the research collaboration. Constructing and operating distributed computing can however be challenging for a smaller-scale ... ...

Abstract	Particle physics experiments rely extensively on computing and data services, making e-infrastructure an integral part of the research collaboration. Constructing and operating distributed computing can however be challenging for a smaller-scale collaboration. The Light Dark Matter eXperiment (LDMX) is a planned small-scale accelerator-based experiment to search for dark matter in the sub-GeV mass region. Finalizing the design of the detector relies on Monte-Carlo simulation of expected physics processes. A distributed computing pilot project was proposed to better utilize available resources at the collaborating institutes, and to improve scalability and reproducibility. This paper outlines the chosen lightweight distributed solution, presenting requirements, the component integration steps, and the experiences using a pilot system for tests with large-scale simulations. The system leverages existing technologies wherever possible, minimizing the need for software development, and deploys only non-intrusive components at the participating sites. The pilot proved that integrating existing components can dramatically reduce the effort needed to build and operate a distributed e-infrastructure, making it attainable even for smaller research collaborations. Comment: 10 pages, 4 figures, Submitted to 25th International Conference on Computing in High-Energy and Nuclear Physics (vCHEP 2021)
Keywords	High Energy Physics - Experiment ; Computer Science - Distributed ; Parallel ; and Cluster Computing
Publishing date	2021-05-06
Publishing country	us
Document type	Book ; Online
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Article ; Online: Combined treatment of L1CAM antibodies and cytostatic drugs improve the therapeutic response of pancreatic and ovarian carcinoma.

Schäfer, Heiner / Dieckmann, Chantal / Korniienko, Olena / Moldenhauer, Gerhard / Kiefel, Helena / Salnikov, Alexey / Krüger, Achim / Altevogt, Peter / Sebens, Susanne

Cancer letters

2012 Volume 319, Issue 1, Page(s) 66–82

Abstract: The adhesion molecule L1CAM (CD171) accounts for enhanced motility, invasiveness and chemoresistance of tumor cells and represents a novel marker for various tumor entities including pancreatic and ovarian carcinoma. Recently, we showed that L1CAM ... ...

Abstract	The adhesion molecule L1CAM (CD171) accounts for enhanced motility, invasiveness and chemoresistance of tumor cells and represents a novel marker for various tumor entities including pancreatic and ovarian carcinoma. Recently, we showed that L1CAM inhibition increases the apoptotic response of tumor cells towards cytostatic drugs pointing to the potential of L1CAM to serve as a chemosensitizer in anti-cancer therapy. Thus, the present study evaluated the therapeutic potential of combined treatment with L1CAM antibodies and chemotherapeutic drugs in pancreatic and ovarian carcinoma model systems in vivo. Two L1CAM-specific antibodies (L1-14.10 and L1-9.3/2a) exhibiting high binding affinity to the L1CAM expressing pancreatic adenocarcinoma cell line Colo357 and the ovarian carcinoma cell line SKOV3ip were used for treatment. The combined therapy of SCID mice with either L1CAM antibody and gemcitabine and paclitaxel, respectively, reduced the growth of subcutaneously grown Colo357 or SKOV3ip tumors more efficiently than treatment with the cytostatic drug alone or in combination with control IgG. This was accompanied by an increased number of apoptotic tumor cells along with an elevated procaspase-8 expression. Furthermore, a lowered activation of NF-κB along with a reduced expression of VEGF and a diminished number of CD31-positive blood vessels were observed in tumors after combined therapy compared to control treatments, while the infiltration of F4/80-positive macrophages increased. Overall, these data provide new insights into the mechanism of the anti-cancer activity of L1CAM-blocking antibodies in vivo and support the suitability of L1CAM as a target for chemosensitization and of L1CAM-interfering antibodies as an appropriate tool to increase the therapeutic response of pancreatic and ovarian carcinoma.
MeSH term(s)	Animals ; Antibodies, Monoclonal/administration & dosage ; Antineoplastic Combined Chemotherapy Protocols/therapeutic use ; Biomarkers, Tumor ; Carcinoma/drug therapy ; Carcinoma, Pancreatic Ductal/drug therapy ; Cell Line, Tumor ; Cytostatic Agents/administration & dosage ; Deoxycytidine/administration & dosage ; Deoxycytidine/analogs & derivatives ; Disease Models, Animal ; Female ; Mice ; Mice, SCID ; Neural Cell Adhesion Molecule L1/antagonists & inhibitors ; Neural Cell Adhesion Molecule L1/immunology ; Ovarian Neoplasms/drug therapy ; Paclitaxel/administration & dosage ; Pancreatic Neoplasms/drug therapy
Chemical Substances	Antibodies, Monoclonal ; Biomarkers, Tumor ; Cytostatic Agents ; Neural Cell Adhesion Molecule L1 ; Deoxycytidine (0W860991D6) ; gemcitabine (B76N6SBZ8R) ; Paclitaxel (P88XT4IS4D)
Language	English
Publishing date	2012-06-01
Publishing country	Ireland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	195674-7
ISSN	1872-7980 ; 0304-3835
ISSN (online)	1872-7980
ISSN	0304-3835
DOI	10.1016/j.canlet.2011.12.035
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Zs.A 1177: Show issues

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Article: Biosintez vitaminov prorastaiushchimi semenami v usloviiakh nizkoĭ temperatury i pereuvlazhneniia pochvy

Salnikov, A I

Prikladnaia biokhimiia i mikrobiologiia

1975 Volume 11, Issue 6, Page(s) 885–887

Abstract: The distribution of farnesene and farnesene hydroperoxide in apples of different varieties was studied. The relationship between the content of these compounds and the pathological browning of the apple surface, scald was followed. It was shown that 1) ... ...

Title translation	Vitamin biosynthesis by germinating seeds at low temperature and in overmoistened soil.
Abstract	The distribution of farnesene and farnesene hydroperoxide in apples of different varieties was studied. The relationship between the content of these compounds and the pathological browning of the apple surface, scald was followed. It was shown that 1) farnesene and farnesene hydroperoxide were concentrated in the cuticle; 2) there was no distinct correlation between farnesene concentration and scald, 3) there was a direct correlation between the concentration of farnese hydroperoxide and scald development; 4) the factors that inhibited farnesene oxidation (antioxidants, low oxygen concentration, mineral oils--farnesene absorbers) slowed down the scald development during apple storage.
MeSH term(s)	Biotin/biosynthesis ; Cold Temperature ; Inositol/biosynthesis ; Nicotinic Acids/biosynthesis ; Pantothenic Acid/biosynthesis ; Plants/drug effects ; Plants/metabolism ; Pyridoxine/biosynthesis ; Seeds/metabolism ; Soil ; Thiamine/biosynthesis ; Triticum/metabolism ; Vitamin B Complex/biosynthesis ; Water/pharmacology
Chemical Substances	Nicotinic Acids ; Soil ; Water (059QF0KO0R) ; Vitamin B Complex (12001-76-2) ; Pantothenic Acid (19F5HK2737) ; Inositol (4L6452S749) ; Biotin (6SO6U10H04) ; Pyridoxine (KV2JZ1BI6Z) ; Thiamine (X66NSO3N35)
Language	Russian
Publishing date	1975-11
Publishing country	Russia (Federation)
Document type	Journal Article
ZDB-ID	412549-6
ISSN	0555-1099
ISSN	0555-1099
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Zs.B 1871: Show issues

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Book: Information management system for SND experiment

Gaponenko, I. A / Salnikov, A. A

(Budker INP ; 98-39)

1998

Title variant	Raspredelennaja sistema obmena informaciej (IMAN) dlja ėksperimenta SND
Author's details	I. A. Gaponenko, A. A. Salnikov
Series title	Budker INP ; 98-39
Language	English
Size	59 S
Publishing place	Novosibirsk
Document type	Book
Note	Russ. Zsfassung u.d.T.: Raspredelennaja sistema obmena informaciej (IMAN) dlja ėksperimenta SND
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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