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  1. Article ; Online: Protocol for the detection of defined T cell clones in a heterogeneous cell population

    Andrea Gobbini / Alessandra Bandera / Renata Grifantini / Sergio Abrignani / Samuele Notarbartolo

    STAR Protocols, Vol 5, Iss 1, Pp 102787- (2024)

    1481  

    Abstract: Summary: Identifying defined T cell clones within a polyclonal population is key to clarifying their phenotype and function. Here, we present a protocol for detecting specified T cell clones in a heterogeneous cell population. We describe steps for ... ...

    Abstract Summary: Identifying defined T cell clones within a polyclonal population is key to clarifying their phenotype and function. Here, we present a protocol for detecting specified T cell clones in a heterogeneous cell population. We describe steps for stimulating human CD4+ T cells isolated from blood with a protein antigen, sorting antigen-specific cells by fluorescence-activated cell sorting, and detecting among these the presence of predefined T cell clones, based on their T cell receptor (TCR). TCR cDNA is amplified through 5′-RACE (TCR-SMART) and detected by qPCR.For complete details on the use and execution of this protocol, please refer to Notarbartolo et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
    Keywords Bioinformatics ; Cell isolation ; Flow Cytometry ; Gene Expression ; Immunology ; Molecular Biology ; Science (General) ; Q1-390
    Language English
    Publishing date 2024-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Transcriptional signature of human pro-inflammatory TH17 cells identifies reduced IL10 gene expression in multiple sclerosis

    Dan Hu / Samuele Notarbartolo / Tom Croonenborghs / Bonny Patel / Ron Cialic / Tun-Hsiang Yang / Dominik Aschenbrenner / Karin M. Andersson / Marco Gattorno / Minh Pham / Pia Kivisakk / Isabelle V. Pierre / Youjin Lee / Karun Kiani / Maria Bokarewa / Emily Tjon / Nathalie Pochet / Federica Sallusto / Vijay K. Kuchroo /
    Howard L. Weiner

    Nature Communications, Vol 8, Iss 1, Pp 1-

    2017  Volume 14

    Abstract: CD4+ T cells secreting interleukin-17 (TH17) have diverse functions in modulating autoimmune diseases. Here the authors show via transcriptome analyses that a subset of human TH 17 co-expressing interferon-γ (TH1/17) has a molecular signature similar to “ ...

    Abstract CD4+ T cells secreting interleukin-17 (TH17) have diverse functions in modulating autoimmune diseases. Here the authors show via transcriptome analyses that a subset of human TH 17 co-expressing interferon-γ (TH1/17) has a molecular signature similar to “pathogenic” mouse TH 17 but distinct from “non-pathogenic” mouse TH 17.
    Keywords Science ; Q
    Language English
    Publishing date 2017-11-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: Transcriptional signature of human pro-inflammatory TH17 cells identifies reduced IL10 gene expression in multiple sclerosis

    Dan Hu / Samuele Notarbartolo / Tom Croonenborghs / Bonny Patel / Ron Cialic / Tun-Hsiang Yang / Dominik Aschenbrenner / Karin M. Andersson / Marco Gattorno / Minh Pham / Pia Kivisakk / Isabelle V. Pierre / Youjin Lee / Karun Kiani / Maria Bokarewa / Emily Tjon / Nathalie Pochet / Federica Sallusto / Vijay K. Kuchroo /
    Howard L. Weiner

    Nature Communications, Vol 8, Iss 1, Pp 1-

    2017  Volume 14

    Abstract: CD4+ T cells secreting interleukin-17 (TH17) have diverse functions in modulating autoimmune diseases. Here the authors show via transcriptome analyses that a subset of human TH 17 co-expressing interferon-γ (TH1/17) has a molecular signature similar to “ ...

    Abstract CD4+ T cells secreting interleukin-17 (TH17) have diverse functions in modulating autoimmune diseases. Here the authors show via transcriptome analyses that a subset of human TH 17 co-expressing interferon-γ (TH1/17) has a molecular signature similar to “pathogenic” mouse TH 17 but distinct from “non-pathogenic” mouse TH 17.
    Keywords Science ; Q
    Language English
    Publishing date 2017-11-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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