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Article ; Online: HyperMRI: hyperspectral and magnetic resonance fusion methodology for neurosurgery applications.

Villa, Manuel / Sancho, Jaime / Rosa, Gonzalo / Chavarrias, Miguel / Juarez, Eduardo / Sanz, Cesar

International journal of computer assisted radiology and surgery

2024

Abstract: Purpose: Magnetic resonance imaging (MRI) is a common technique in image-guided neurosurgery (IGN). Recent research explores the integration of methods like ultrasound and tomography, among others, with hyperspectral (HS) imaging gaining attention due ... ...

Abstract	Purpose: Magnetic resonance imaging (MRI) is a common technique in image-guided neurosurgery (IGN). Recent research explores the integration of methods like ultrasound and tomography, among others, with hyperspectral (HS) imaging gaining attention due to its non-invasive real-time tissue classification capabilities. The main challenge is the registration process, often requiring manual intervention. This work introduces an automatic, markerless method for aligning HS images with MRI. Methods: This work presents a multimodal system that combines RGB-Depth (RGBD) and HS cameras. The RGBD camera captures the patient's facial geometry, which is used for registration with the preoperative MR through ICP. Once MR-depth registration is complete, the integration of HS data is achieved using a calibrated homography transformation. The incorporation of external tracking with a novel calibration method allows camera mobility from the registration position to the craniotomy area. This methodology streamlines the fusion of RGBD, HS and MR images within the craniotomy area. Results: Using the described system and an anthropomorphic phantom head, the system has been characterised by registering the patient's face in 25 positions and 5 positions resulted in a fiducial registration error of 1.88 ± 0.19 mm and a target registration error of 4.07 ± 1.28 mm, respectively. Conclusions: This work proposes a new methodology to automatically register MR and HS information with a sufficient accuracy. It can support the neurosurgeons to guide the diagnosis using multimodal data over an augmented reality representation. However, in its preliminary prototype stage, this system exhibits significant promise, driven by its cost-effectiveness and user-friendly design.
Language	English
Publishing date	2024-05-18
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2365628-1
ISSN	1861-6429 ; 1861-6410
ISSN (online)	1861-6429
ISSN	1861-6410
DOI	10.1007/s11548-024-03102-5
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Article ; Online: Alterations in the Glycan Profile of Mouse Transferrin: New Insights in Collagen-Induced Arthritis.

Mancera-Arteu, Montserrat / Giménez, Estela / Sancho, Jaime / Sanz-Nebot, Victoria

Journal of proteome research

2020 Volume 19, Issue 4, Page(s) 1750–1759

Abstract: Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC) was optimized in order to study the possible modifications occurring in its glycans in collagen-induced arthritis (CIA) samples. SDS-PAGE and nanoLC-MS/MS were used ... ...

Abstract	Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC) was optimized in order to study the possible modifications occurring in its glycans in collagen-induced arthritis (CIA) samples. SDS-PAGE and nanoLC-MS/MS were used to monitor the IAC purification performance. Afterward, a relative quantification of mouse transferrin (mTf) glycan isomers using [
MeSH term(s)	Animals ; Arthritis, Experimental ; Glycosylation ; Mice ; Polysaccharides ; Tandem Mass Spectrometry ; Transferrin
Chemical Substances	Polysaccharides ; Transferrin
Language	English
Publishing date	2020-03-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2078618-9
ISSN	1535-3907 ; 1535-3893
ISSN (online)	1535-3907
ISSN	1535-3893
DOI	10.1021/acs.jproteome.0c00016
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Zs.A 6189: Show issues

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Article ; Online: GoRG: Towards a GPU-Accelerated Multiview Hyperspectral Depth Estimation Tool for Medical Applications.

Sancho, Jaime / Sutradhar, Pallab / Rosa, Gonzalo / Chavarrías, Miguel / Perez-Nuñez, Angel / Salvador, Rubén / Lagares, Alfonso / Juárez, Eduardo / Sanz, César

Sensors (Basel, Switzerland)

2021 Volume 21, Issue 12

Abstract: HyperSpectral (HS) images have been successfully used for brain tumor boundary detection during resection operations. Nowadays, these classification maps coexist with other technologies such as MRI or IOUS that improve a neurosurgeon's action, with their ...

Abstract	HyperSpectral (HS) images have been successfully used for brain tumor boundary detection during resection operations. Nowadays, these classification maps coexist with other technologies such as MRI or IOUS that improve a neurosurgeon's action, with their incorporation being a neurosurgeon's task. The project in which this work is framed generates an unified and more accurate 3D immersive model using HS, MRI, and IOUS information. To do so, the HS images need to include 3D information and it needs to be generated in real-time operating room conditions, around a few seconds. This work presents Graph cuts Reference depth estimation in GPU (GoRG), a GPU-accelerated multiview depth estimation tool for HS images also able to process YUV images in less than 5.5 s on average. Compared to a high-quality SoA algorithm, MPEG DERS, GoRG YUV obtain quality losses of -0.93 dB, -0.6 dB, and -1.96% for WS-PSNR, IV-PSNR, and VMAF, respectively, using a video synthesis processing chain. For HS test images, GoRG obtains an average RMSE of 7.5 cm, with most of its errors in the background, needing around 850 ms to process one frame and view. These results demonstrate the feasibility of using GoRG during a tumor resection operation.
MeSH term(s)	Algorithms ; Brain ; Brain Neoplasms ; Humans ; Magnetic Resonance Imaging
Language	English
Publishing date	2021-06-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s21124091
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Article ; Online: CD38 promotes hematopoietic stem cell dormancy.

PLoS biology

2024 Volume 22, Issue 2, Page(s) e3002517

Abstract: A subpopulation of deeply quiescent, so-called dormant hematopoietic stem cells (dHSCs) resides at the top of the hematopoietic hierarchy and serves as a reserve pool for HSCs. The state of dormancy protects the HSC pool from exhaustion throughout life; ... ...

Abstract	A subpopulation of deeply quiescent, so-called dormant hematopoietic stem cells (dHSCs) resides at the top of the hematopoietic hierarchy and serves as a reserve pool for HSCs. The state of dormancy protects the HSC pool from exhaustion throughout life; however, excessive dormancy may prevent an efficient response to hematological stresses. Despite the significance of dHSCs, the mechanisms maintaining their dormancy remain elusive. Here, we identify CD38 as a novel and broadly applicable surface marker for the enrichment of murine dHSCs. We demonstrate that cyclic adenosine diphosphate ribose (cADPR), the product of CD38 cyclase activity, regulates the expression of the transcription factor c-Fos by increasing the release of Ca2+ from the endoplasmic reticulum (ER). Subsequently, we uncover that c-Fos induces the expression of the cell cycle inhibitor p57Kip2 to drive HSC dormancy. Moreover, we found that CD38 ecto-enzymatic activity at the neighboring CD38-positive cells can promote human HSC quiescence. Together, CD38/cADPR/Ca2+/c-Fos/p57Kip2 axis maintains HSC dormancy. Pharmacological manipulations of this pathway can provide new strategies to improve the success of stem cell transplantation and blood regeneration after injury or disease.
MeSH term(s)	Animals ; Humans ; Mice ; Calcium/metabolism ; Cyclic ADP-Ribose/metabolism ; Hematopoietic Stem Cells ; ADP-ribosyl Cyclase 1/metabolism ; Cyclin-Dependent Kinase Inhibitor p57/metabolism
Chemical Substances	Calcium (SY7Q814VUP) ; Cyclic ADP-Ribose (119340-53-3) ; ADP-ribosyl Cyclase 1 (EC 3.2.2.6) ; CD38 protein, human (EC 3.2.2.5) ; Cd38 protein, mouse (EC 3.2.2.5) ; Cyclin-Dependent Kinase Inhibitor p57
Language	English
Publishing date	2024-02-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	2126776-5
ISSN	1545-7885 ; 1544-9173
ISSN (online)	1545-7885
ISSN	1544-9173
DOI	10.1371/journal.pbio.3002517
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Zs.A 6193: Show issues

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Article ; Online: The Role of CD38 on the Function of Regulatory B Cells in a Murine Model of Lupus.

Burlock, Brianna / Richardson, Gabrielle / García-Rodríguez, Sonia / Guerrero, Salvador / Zubiaur, Mercedes / Sancho, Jaime

International journal of molecular sciences

2018 Volume 19, Issue 10

Abstract: Previous work from our group has shown ... ...

Abstract	Previous work from our group has shown that
MeSH term(s)	ADP-ribosyl Cyclase 1/genetics ; ADP-ribosyl Cyclase 1/immunology ; Animals ; Autoimmunity ; B-Lymphocytes, Regulatory/immunology ; B-Lymphocytes, Regulatory/pathology ; Dendritic Cells/immunology ; Dendritic Cells/pathology ; Disease Models, Animal ; Gene Deletion ; Interferon Type I/immunology ; Interleukin-10/immunology ; Lupus Erythematosus, Systemic/immunology ; Lupus Erythematosus, Systemic/pathology ; Membrane Glycoproteins/genetics ; Membrane Glycoproteins/immunology ; Mice ; Mice, Inbred C57BL
Chemical Substances	Interferon Type I ; Membrane Glycoproteins ; Interleukin-10 (130068-27-8) ; Cd38 protein, mouse (EC 3.2.2.5) ; ADP-ribosyl Cyclase 1 (EC 3.2.2.6)
Language	English
Publishing date	2018-09-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms19102906
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Article ; Online: Reduction of ventilatory time using the multidisciplinary disconnection protocol. Pilot study.

Sánchez-Maciá, Miriam / Miralles-Sancho, Jaime / Castaño-Picó, María José / Pérez-Carbonell, Ana / Maciá-Soler, Loreto

Revista latino-americana de enfermagem

2019 Volume 27, Page(s) e3215

Abstract: Objective: compare ventilatory time between patients with the application of a disconnection protocol, managed in a coordinated way between doctor and nurse, with patients managed exclusively by the doctor.: Method: experimental pilot study before ... ...

Abstract	Objective: compare ventilatory time between patients with the application of a disconnection protocol, managed in a coordinated way between doctor and nurse, with patients managed exclusively by the doctor. Method: experimental pilot study before and after. Twenty-five patients requiring invasive mechanical ventilation for 24 hours or more were included, and the protocol-guided group was compared with the protocol-free group managed according to usual practice. Results: by means of the multidisciplinary protocol, the time of invasive mechanical ventilation was reduced (141.94 ± 114.50 vs 113.18 ± 55.14; overall decrease of almost 29 hours), the time spent on weaning (24 hours vs 7.40 hours) and the numbers of reintubation (13% vs 0%) in comparison with the group in which the nurse did not participate. The time to weaning was shorter in the retrospective cohort (2 days vs. 5 days), as was the hospital stay (7 days vs. 9 days). Conclusion: the use of a multidisciplinary protocol reduces the duration of weaning, the total time of invasive mechanical ventilation and reintubations. The more active role of the nurse is a fundamental tool to obtain better results.
MeSH term(s)	Aged ; Clinical Protocols ; Evidence-Based Practice ; Female ; Humans ; Male ; Nurse's Role ; Physician-Nurse Relations ; Pilot Projects ; Respiration, Artificial/standards ; Retrospective Studies ; Ventilator Weaning/standards
Language	Spanish
Publishing date	2019-12-05
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2105698-5
ISSN	1518-8345 ; 0104-1169
ISSN (online)	1518-8345
ISSN	0104-1169
DOI	10.1590/1518-8345.2923.3215
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Article ; Online: Evaluation of ion mobility for the separation of glycoconjugate isomers due to different types of sialic acid linkage, at the intact glycoprotein, glycopeptide and glycan level.

Barroso, Albert / Giménez, Estela / Konijnenberg, Albert / Sancho, Jaime / Sanz-Nebot, Victoria / Sobott, Frank

Journal of proteomics

2017 Volume 173, Page(s) 22–31

Abstract: The study of protein glycosylation can be regarded as an intricate but very important task, making glycomics one of the most challenging and interesting, albeit under-researched, type of "omics" science. Complexity escalates remarkably when considering ... ...

Abstract	The study of protein glycosylation can be regarded as an intricate but very important task, making glycomics one of the most challenging and interesting, albeit under-researched, type of "omics" science. Complexity escalates remarkably when considering that carbohydrates can form severely branched structures with many different constituents, which often leads to the formation of multiple isomers. In this regard, ion mobility (IM) spectrometry has recently demonstrated its power for the separation of isomeric compounds. In the present work, the potential of traveling wave IM (TWIMS) for the separation of isomeric glycoconjugates was evaluated, using mouse transferrin (mTf) as model glycoprotein. Particularly, we aim to assess the performance of this platform for the separation of isomeric glycoconjugates due to the type of sialic acid linkage, at the intact glycoprotein, glycopeptide and glycan level. Straightforward separation of isomers was achieved with the analysis of released glycans, as opposed to the glycopeptides which showed a more complex pattern. Finally, the developed methodology was applied to serum samples of mice, to investigate its robustness when analyzing real complex samples. Biological significance: Ion mobility mass spectrometry is a promising analytical technique for the separation of glycoconjugate isomers due to type of sialic acid linkage. The impact of such a small modification in the glycan structure is more evident in smaller analytes, reason why the analysis of free glycans was easier compared to the intact protein or the glycopeptides. The established methodology could be regarded as starting point in the separation of highly decorated glycoconjugates. This is an important topic nowadays, as differences in the abundance of some glycan isomers could be the key for the early diagnosis, control or differentiation of certain diseases, such as inflammation or cancer.
MeSH term(s)	Animals ; Glycomics/methods ; Glycosylation ; Ion Mobility Spectrometry/methods ; Ion Mobility Spectrometry/standards ; Isomerism ; Mice ; N-Acetylneuraminic Acid/chemical synthesis ; Polysaccharides/analysis ; Transferrin/chemistry
Chemical Substances	Polysaccharides ; Transferrin ; N-Acetylneuraminic Acid (GZP2782OP0)
Language	English
Publishing date	2017-11-29
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2400835-7
ISSN	1876-7737 ; 1874-3919
ISSN (online)	1876-7737
ISSN	1874-3919
DOI	10.1016/j.jprot.2017.11.020
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Article ; Online: Tocilizumab as an Adjuvant Therapy for Hemophagocytic Lymphohistiocytosis Associated With Visceral Leishmaniasis.

Rios-Fernández, Raquel / Callejas-Rubio, Jose-Luis / García-Rodríguez, Sonia / Sancho, Jaime / Zubiaur, Mercedes / Ortego-Centeno, Norberto

American journal of therapeutics

2016 Volume 23, Issue 5, Page(s) e1193–6

Abstract: Leishmaniasis is important as a cause of hemophagocytic lymphohistiocytosis (HLH) and must be considered and excluded in patients with HLH because it can cause severe or even fatal complications. When HLH is present, there is a deficient downregulation ... ...

Abstract	Leishmaniasis is important as a cause of hemophagocytic lymphohistiocytosis (HLH) and must be considered and excluded in patients with HLH because it can cause severe or even fatal complications. When HLH is present, there is a deficient downregulation of the immune response, leading to an uncontrolled inflammation. We report a case of visceral leishmaniasis-HLH where the therapy with tocilizumab, targeting interleukin 6, help to regulate the immune response for the infection of Leishmania.
MeSH term(s)	Adult ; Antibodies, Monoclonal, Humanized/administration & dosage ; Antibodies, Monoclonal, Humanized/pharmacology ; Antibodies, Monoclonal, Humanized/therapeutic use ; Female ; Humans ; Leishmaniasis, Visceral/complications ; Leishmaniasis, Visceral/drug therapy ; Leishmaniasis, Visceral/immunology ; Lymphohistiocytosis, Hemophagocytic/drug therapy ; Lymphohistiocytosis, Hemophagocytic/etiology ; Lymphohistiocytosis, Hemophagocytic/immunology ; Receptors, Interleukin-6/antagonists & inhibitors ; Receptors, Interleukin-6/immunology
Chemical Substances	Antibodies, Monoclonal, Humanized ; Receptors, Interleukin-6 ; tocilizumab (I031V2H011)
Language	English
Publishing date	2016-09
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	1280786-2
ISSN	1536-3686 ; 1075-2765
ISSN (online)	1536-3686
ISSN	1075-2765
DOI	10.1097/MJT.0000000000000035
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Zs.A 4351: Show issues

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Article: Cutting edge: natural DNA repetitive extragenic sequences from gram-negative pathogens strongly stimulate TLR9.

Magnusson, Mattias / Tobes, Raquel / Sancho, Jaime / Pareja, Eduardo

Journal of immunology (Baltimore, Md. : 1950)

2007 Volume 179, Issue 1, Page(s) 31–35

Abstract: Bacterial DNA exerts immunostimulatory effects on mammalian cells via the intracellular TLR9. Although broad analysis of TLR9-mediated immunostimulatory potential of synthetic oligonucleotides has been developed, which kinds of natural bacterial DNA ... ...

Abstract	Bacterial DNA exerts immunostimulatory effects on mammalian cells via the intracellular TLR9. Although broad analysis of TLR9-mediated immunostimulatory potential of synthetic oligonucleotides has been developed, which kinds of natural bacterial DNA sequences are responsible for immunostimulation are not known. This work provides evidence that the natural DNA sequences named repetitive extragenic palindromic (REPs) sequences present in Gram-negative bacteria are able to produce innate immune system stimulation via TLR9. A strong induction of IFN-alpha production by REPs from Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa, and Neisseria meningitidis was detected in splenocytes from 129 mice. In addition, the involvement of TLR9 in immune stimulation by REPs was confirmed using B6.129P2-Tlr9(tm1Aki) knockout mice. Considering the involvement of TLRs in Gram-negative septic shock, it is conceivable that REPs play a role in its pathogenesis. This study highlights REPs as a potential novel target in septic shock treatment.
MeSH term(s)	Adjuvants, Immunologic/genetics ; Adjuvants, Immunologic/physiology ; Animals ; Cells, Cultured ; DNA, Bacterial/metabolism ; DNA, Bacterial/physiology ; Escherichia coli K12/genetics ; Gram-Negative Bacteria/genetics ; Gram-Negative Bacteria/immunology ; Humans ; Immunity, Innate/genetics ; Ligands ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Neisseria meningitidis, Serogroup B/genetics ; Pseudomonas aeruginosa/genetics ; Repetitive Sequences, Nucleic Acid ; Salmonella typhi/genetics ; Toll-Like Receptor 9/deficiency ; Toll-Like Receptor 9/genetics ; Toll-Like Receptor 9/metabolism
Chemical Substances	Adjuvants, Immunologic ; DNA, Bacterial ; Ligands ; Toll-Like Receptor 9
Language	English
Publishing date	2007-04-16
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3056-9
ISSN	1550-6606 ; 0022-1767 ; 1048-3233 ; 1047-7381
ISSN (online)	1550-6606
ISSN	0022-1767 ; 1048-3233 ; 1047-7381
DOI	10.4049/jimmunol.179.1.31
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Zs.MG 28: Show issues

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Article ; Online: Extracellular vesicles from pristane-treated CD38-deficient mice express an anti-inflammatory neutrophil protein signature, which reflects the mild lupus severity elicited in these mice.

Carrillo-Rodríguez, Paula / Robles-Guirado, José-Ángel / Cruz-Palomares, Adrián / Palacios-Pedrero, Miguel Ángel / González-Paredes, Elena / Más-Ciurana, Alex / Franco-Herrera, Carolina / Ruiz-de-Castroviejo-Teba, Paloma A / Lario, Antonio / Longobardo, Victoria / Montosa-Hidalgo, Laura / Pérez-Sánchez-Cañete, María M / Corzo-Corbera, María-Mercedes / Redondo-Sánchez, Sandra / Jodar, Ana-Belén / Blanco, Francisco J / Zumaquero, Esther / Merino, Ramón / Sancho, Jaime /

Zubiaur, Mercedes

Frontiers in immunology

2022 Volume 13, Page(s) 1013236

Abstract: In CD38-deficient ( ... Cd38 ... -/- ... ) ... mice intraperitoneal injection of pristane induces a lupus-like disease, which is milder than that induced in WT mice, showing significant differences in the inflammatory and autoimmune processes ... ...

Abstract	In CD38-deficient ( Cd38<sup>-/-</sup> ) mice intraperitoneal injection of pristane induces a lupus-like disease, which is milder than that induced in WT mice, showing significant differences in the inflammatory and autoimmune processes triggered by pristane. Extracellular vesicles (EV) are present in all body fluids. Shed by cells, their molecular make-up reflects that of their cell of origin and/or tissue pathological situation. The aim of this study was to analyze the protein composition, protein abundance, and functional clustering of EV released by peritoneal exudate cells (PECs) in the pristane experimental lupus model, to identify predictive or diagnostic biomarkers that might discriminate the autoimmune process in lupus from inflammatory reactions and/or normal physiological processes. In this study, thanks to an extensive proteomic analysis and powerful bioinformatics software, distinct EV subtypes were identified in the peritoneal exudates of pristane-treated mice: 1) small EV enriched in the tetraspanin CD63 and CD9, which are likely of exosomal origin; 2) small EV enriched in CD47 and CD9, which are also enriched in plasma-membrane, membrane-associated proteins, with an ectosomal origin; 3) small EV enriched in keratins, ECM proteins, complement/coagulation proteins, fibrin clot formation proteins, and endopetidase inhibitor proteins. This enrichment may have an inflammation-mediated mesothelial-to-mesenchymal transition origin, representing a protein corona on the surface of peritoneal exudate EV; 4) HDL-enriched lipoprotein particles. Quantitative proteomic analysis allowed us to identify an anti-inflammatory, Annexin A1-enriched pro-resolving, neutrophil protein signature, which was more prominent in EV from pristane-treated Cd38<sup>-/-</sup> mice, and quantitative differences in the protein cargo of the ECM-enriched EV from Cd38<sup>-/-</sup> vs WT mice. These differences are likely to be related with the distinct inflammatory outcome shown by Cd38<sup>-/-</sup> vs WT mice in response to pristane treatment. Our results demonstrate the power of a hypothesis-free and data-driven approach to transform the heterogeneity of the peritoneal exudate EV from pristane-treated mice in valuable information about the relative proportion of different EV in a given sample and to identify potential protein markers specific for the different small EV subtypes, in particular those proteins defining EV involved in the resolution phase of chronic inflammation.
MeSH term(s)	Mice ; Animals ; Neutrophils ; Proteomics ; Disease Models, Animal ; Inflammation ; Anti-Inflammatory Agents ; Extracellular Vesicles
Chemical Substances	pristane (26HZV48DT1) ; Anti-Inflammatory Agents
Language	English
Publishing date	2022-10-24
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.1013236
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