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  1. Article: Determination of single-cell oxygen consumption with impedance feedback for control of sample-probe separation.

    Osbourn, Damon M / Sanger, Richard H / Smith, Peter J S

    Analytical chemistry

    2005  Volume 77, Issue 21, Page(s) 6999–7004

    Abstract: The ability to measure chemical gradients surrounding single cells provides novel insights into several areas of cell dynamics--particularly metabolism. Detection of metabolic oxygen consumption can be achieved from a single mammalian cell using a ... ...

    Abstract The ability to measure chemical gradients surrounding single cells provides novel insights into several areas of cell dynamics--particularly metabolism. Detection of metabolic oxygen consumption can be achieved from a single mammalian cell using a modulated amperometric sensor in a self-referencing mode. To date, however, apart from visual cues, we do not have a reliable and cell-compatible method for determining and stabilizing the position of such probes. In this paper, we report on having successfully measured the increase in the uncompensated resistance of an electrochemical cell upon approach to single, living, biological cells, while simultaneously measuring the metabolic oxygen consumption. This was accomplished by applying an ac and a dc excitation signal to the electrode. The applied ac waveform was a 100-kHz sine wave with an amplitude of 10 mV rms, while the dc voltage applied was -600 mV. The two signals were shown not to interfere with one another. Furthermore, it is shown that the sample-probe distance can be measured for approach to single cells on the order of 10-15-microm diameter and 5-microm height, with 100-nm resolution.
    MeSH term(s) Animals ; Cell Line ; Cytological Techniques/instrumentation ; Cytological Techniques/methods ; Electric Impedance ; Insulinoma ; Oxygen Consumption/physiology ; Rats
    Language English
    Publishing date 2005-11-01
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/ac050326w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4033: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
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  2. Article: Libya

    Sanger, Richard H

    The Middle East journal Vol. 29, No. 4 , p. 409-417

    conclusions on an unfinished revolution

    1975  Volume 29, Issue 4, Page(s) 409–417

    Author's details Richard H. Sanger
    Keywords Staatsumwälzung ; Libyen
    Publisher Inst.
    Publishing place Washington, DC
    Document type Article
    ZDB-ID 3507-5
    Database ECONomics Information System

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  3. Article ; Online: Bcl-xL induces Drp1-dependent synapse formation in cultured hippocampal neurons.

    Li, Hongmei / Chen, Yingbei / Jones, Adrienne F / Sanger, Richard H / Collis, Leon P / Flannery, Richard / McNay, Ewan C / Yu, Tingxi / Schwarzenbacher, Robert / Bossy, Blaise / Bossy-Wetzel, Ella / Bennett, Michael V L / Pypaert, Marc / Hickman, John A / Smith, Peter J S / Hardwick, J Marie / Jonas, Elizabeth A

    Proceedings of the National Academy of Sciences of the United States of America

    2008  Volume 105, Issue 6, Page(s) 2169–2174

    Abstract: Maturation of neuronal synapses is thought to involve mitochondria. Bcl-xL protein inhibits mitochondria-mediated apoptosis but may have other functions in healthy adult neurons in which Bcl-xL is abundant. Here, we report that overexpression of Bcl-xL ... ...

    Abstract Maturation of neuronal synapses is thought to involve mitochondria. Bcl-xL protein inhibits mitochondria-mediated apoptosis but may have other functions in healthy adult neurons in which Bcl-xL is abundant. Here, we report that overexpression of Bcl-xL postsynaptically increases frequency and amplitude of spontaneous miniature synaptic currents in rat hippocampal neurons in culture. Bcl-xL, overexpressed either pre or postsynaptically, increases synapse number, the number and size of synaptic vesicle clusters, and mitochondrial localization to vesicle clusters and synapses, likely accounting for the changes in miniature synaptic currents. Conversely, knockdown of Bcl-xL or inhibiting it with ABT-737 decreases these morphological parameters. The mitochondrial fission protein, dynamin-related protein 1 (Drp1), is a GTPase known to localize to synapses and affect synaptic function and structure. The effects of Bcl-xL appear mediated through Drp1 because overexpression of Drp1 increases synaptic markers, and overexpression of the dominant-negative dnDrp1-K38A decreases them. Furthermore, Bcl-xL coimmunoprecipitates with Drp1 in tissue lysates, and in a recombinant system, Bcl-xL protein stimulates GTPase activity of Drp1. These findings suggest that Bcl-xL positively regulates Drp1 to alter mitochondrial function in a manner that stimulates synapse formation.
    MeSH term(s) Animals ; Cells, Cultured ; Dynamins/physiology ; Hippocampus/cytology ; Hippocampus/metabolism ; Mitochondria/metabolism ; Rats ; Synapses ; Synaptic Transmission ; bcl-X Protein/physiology
    Chemical Substances bcl-X Protein ; Dnm1l protein, rat (EC 3.6.5.5) ; Dynamins (EC 3.6.5.5)
    Language English
    Publishing date 2008-02-04
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.0711647105
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1148: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article: Bcl-xL induces Drp1-dependent synapse formation in cultured hippocampal neurons

    Li, Hongmei / Chen, Yingbei / Jones, Adrienne F / Sanger, Richard H / Collis, Leon P / Flannery, Richard / McNay, Ewan C / Yu, Tingxi / Schwarzenbacher, Robert / Bossy, Blaise / Bossy-Wetzel, Ella / Bennett, Michael V.L / Pypaert, Marc / Hickman, John A / Smith, Peter J.S / Hardwick, J. Marie / Jonas, Elizabeth A

    Proceedings of the National Academy of Sciences of the United States of America. 2008 Feb. 12, v. 105, no. 6

    2008  

    Abstract: Maturation of neuronal synapses is thought to involve mitochondria. Bcl-xL protein inhibits mitochondria-mediated apoptosis but may have other functions in healthy adult neurons in which Bcl-xL is abundant. Here, we report that overexpression of Bcl-xL ... ...

    Abstract Maturation of neuronal synapses is thought to involve mitochondria. Bcl-xL protein inhibits mitochondria-mediated apoptosis but may have other functions in healthy adult neurons in which Bcl-xL is abundant. Here, we report that overexpression of Bcl-xL postsynaptically increases frequency and amplitude of spontaneous miniature synaptic currents in rat hippocampal neurons in culture. Bcl-xL, overexpressed either pre or postsynaptically, increases synapse number, the number and size of synaptic vesicle clusters, and mitochondrial localization to vesicle clusters and synapses, likely accounting for the changes in miniature synaptic currents. Conversely, knockdown of Bcl-xL or inhibiting it with ABT-737 decreases these morphological parameters. The mitochondrial fission protein, dynamin-related protein 1 (Drp1), is a GTPase known to localize to synapses and affect synaptic function and structure. The effects of Bcl-xL appear mediated through Drp1 because overexpression of Drp1 increases synaptic markers, and overexpression of the dominant-negative dnDrp1-K38A decreases them. Furthermore, Bcl-xL coimmunoprecipitates with Drp1 in tissue lysates, and in a recombinant system, Bcl-xL protein stimulates GTPase activity of Drp1. These findings suggest that Bcl-xL positively regulates Drp1 to alter mitochondrial function in a manner that stimulates synapse formation.
    Language English
    Dates of publication 2008-0212
    Size p. 2169-2174.
    Publishing place National Academy of Sciences
    Document type Article
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 4' 63/44: Show issues
    87/25270: Show issues
    Qa 4' 169/3: Show issues
    Z 8.7: Show issues

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  5. Article: American policy in the Middle East during 1953

    Sanger, Richard H

    The Department of State bulletin : the official weekly record of United States Foreign Policy Vol. 30, No. 763 , p. 209-214

    1954  Volume 30, Issue 763, Page(s) 209–214

    Author's details Richard H. Sanger
    Keywords Aussenpolitik ; Vereinigte Staaten ; Mittlerer und Naher Osten
    Publisher US Gov.Print.Off.
    Publishing place Washington, DC
    Document type Article
    ZDB-ID 212461-0
    Database ECONomics Information System

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