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Article ; Online: Canine urothelial carcinoma: a pilot study of microRNA detection in formalin-fixed, paraffin-embedded tissue samples and in normal urine

Varvil, Mara S. / Clark, Samuel L. / Bailey, Taylor W. / Ramos-Vara, José A. / Santos, Andrea Pires dos

Journal of Veterinary Diagnostic Investigation. 2024 Jan., v. 36, no. 1 p.70-77

2024

Abstract: We assessed the effects of fixation time in formalin and inclusion of surrounding tissue on microRNA (miRNA) cycle quantification (Cq) values in formalin-fixed, paraffin-embedded (FFPE) urothelial carcinoma (UC) tissue (n = 3), and the effect of ... ...

Abstract	We assessed the effects of fixation time in formalin and inclusion of surrounding tissue on microRNA (miRNA) cycle quantification (Cq) values in formalin-fixed, paraffin-embedded (FFPE) urothelial carcinoma (UC) tissue (n = 3), and the effect of conditions on miRNAs in urine from 1 healthy dog. MiRNAs were extracted using commercial kits and quantified using miRNA-specific fluorometry in normal bladder tissue scrolls, UC tissue cores, and bladder muscularis tissue cores from 4 FFPE bladder sections (3 UCs, 1 normal), plus 1 UC stored in formalin for 1, 8, 15, and 22 d before paraffin-embedding. Urine was collected from a healthy dog on 4 occasions; 1-mL aliquots were stored at 20, 4, −20, and −80°C for 4, 8, 24, and 48 h, and 1 and 2 wk. For both FFPE tissue and urine, we used reverse-transcription quantitative real-time PCR (RT-qPCR) to quantify miR-143, miR-152, miR-181a, miR-214, miR-1842, and RNU6B in each tissue or sample, using miR-39 as an exogenous control gene. The Cq values were compared with ANOVA and t-tests. The time of tissue-fixation in formalin did not alter miRNA Cq values; inclusion of the muscularis layer resulted in a statistically different miRNA Cq profile for miR-152, miR-181a, and RNU6B in bladder tissue. MiRNAs in acellular urine were stable for up to 2 wk regardless of the storage temperature. Our findings support using stored FFPE and urine samples for miRNA detection; we recommend measuring miRNA only in the tissue of interest in FFPE sections.
Keywords	bladder ; carcinoma ; dogs ; fluorometry ; formalin ; genes ; microRNA ; quantitative polymerase chain reaction ; reverse transcription ; storage temperature ; t-test ; urine ; formalin-fixation ; paraffin-embedded tissues ; stability ; urothelial carcinoma
Language	English
Dates of publication	2024-01
Size	p. 70-77.
Publishing place	SAGE Publications
Document type	Article ; Online
ZDB-ID	287603-6
ISSN	1943-4936 ; 1040-6387
ISSN (online)	1943-4936
ISSN	1040-6387
DOI	10.1177/10406387231211908
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Canine urothelial carcinoma: a pilot study of microRNA detection in formalin-fixed, paraffin-embedded tissue samples and in normal urine.

Varvil, Mara S / Clark, Samuel L / Bailey, Taylor W / Ramos-Vara, José A / Santos, Andrea Pires Dos

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

2023 Volume 36, Issue 1, Page(s) 70–77

Abstract	We assessed the effects of fixation time in formalin and inclusion of surrounding tissue on microRNA (miRNA) cycle quantification (Cq) values in formalin-fixed, paraffin-embedded (FFPE) urothelial carcinoma (UC) tissue (
MeSH term(s)	Dogs ; Animals ; MicroRNAs/genetics ; MicroRNAs/analysis ; Pilot Projects ; Carcinoma, Transitional Cell/genetics ; Carcinoma, Transitional Cell/veterinary ; Urinary Bladder Neoplasms/veterinary ; Paraffin Embedding/veterinary ; Formaldehyde ; Tissue Fixation/veterinary ; Tissue Fixation/methods ; Dog Diseases/diagnosis ; Dog Diseases/genetics ; Dog Diseases/pathology
Chemical Substances	MicroRNAs ; Formaldehyde (1HG84L3525)
Language	English
Publishing date	2023-11-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	287603-6
ISSN	1943-4936 ; 1040-6387
ISSN (online)	1943-4936
ISSN	1040-6387
DOI	10.1177/10406387231211908
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Article ; Online: Dog and Cat Contact as Risk Factor for Human Toxocariasis: Systematic Review and Meta-Analysis.

Merigueti, Yslla Fernanda Fitz Balo / Giuffrida, Rogerio / da Silva, Rodrigo Costa / Kmetiuk, Louise Bach / Santos, Andrea Pires Dos / Biondo, Alexander Welker / Santarém, Vamilton Alvares

Frontiers in public health

2022 Volume 10, Page(s) 854468

Abstract: Toxocariasis, a neglected parasitic zoonosis with worldwide distribution, has been reportedly associated to different risk factors in several epidemiological and meta-analysis studies. However, dog and cat contact (environmental and animal exposure) as ... ...

Abstract	Toxocariasis, a neglected parasitic zoonosis with worldwide distribution, has been reportedly associated to different risk factors in several epidemiological and meta-analysis studies. However, dog and cat contact (environmental and animal exposure) as isolated associated risk factor for children and adults remains to be fully established. Accordingly, the present meta-analysis has aimed to directly assess dog and cat contact for toxocariasis seropositivity in under-18 and adult persons, using a survey strategy of PubMed/Medline, Embase, Scopus and Scielo Databases, from January 2009 to December 2021. A meta-analysis model of random effects was applied to estimate
MeSH term(s)	Adult ; Animals ; Cat Diseases/epidemiology ; Cat Diseases/parasitology ; Cats ; Child ; Dog Diseases/parasitology ; Dogs ; Humans ; Risk Factors ; Toxocara ; Toxocariasis/epidemiology ; Toxocariasis/parasitology ; United States
Language	English
Publishing date	2022-06-28
Publishing country	Switzerland
Document type	Meta-Analysis ; Systematic Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2711781-9
ISSN	2296-2565 ; 2296-2565
ISSN (online)	2296-2565
ISSN	2296-2565
DOI	10.3389/fpubh.2022.854468
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Molecular detection of feline hemoplasmas and retroviruses in free-roaming and shelter cats within a university campus [Mar. 2023, 9(1), p. 20551169231162671]

Yamakawa, Ana Carolina / Haisi, Amanda / Kmetiuk, Louise Bach / Pellizzaro, Maysa / Mendes, Juliana Cristina Rebonato / Canavessi, Aurea Maria Oliveira / Ullmann, Leila Sabrina / de Castro, Wagner Antônio Chiba / Pessoa Araújo Júnior, João / Santos, Andrea Pires dos / Biondo, Alexander Welker

Journal of Feline Medicine and Surgery Open Reports. 2023 Feb., v. 9, no. 1, p. 20551169221148672

2023 , Page(s) 20551169221148672

Abstract: The aim of the present study was to assess the frequency of hemoplasma, feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) infections in cats living in an on-campus shelter and free-roaming cats within a university campus in Brazil. ... ...

Abstract	The aim of the present study was to assess the frequency of hemoplasma, feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) infections in cats living in an on-campus shelter and free-roaming cats within a university campus in Brazil. Blood samples were tested using quantitative PCR for hemoplasma, FIV and FeLV. Positive hemoplasma samples were sequenced. Associations between hemoplasma detection and living situation, sex, flea and/or tick parasitism, and coinfection with FIV and FeLV, were assessed using Fisher’s exact test and the respective odds ratios were calculated. Overall, 6/45 (13.3%) cats tested positive: four (8.9%) were infected with ‘Candidatus Mycoplasma haemominutum’ and two (4.4%) with Mycoplasma haemofelis. All positive samples were from free-roaming cats (6/15; 40.0%) and had statistically significantly lower packed cell volumes (P = 0.037). Although 5/23 (21.7%) males and 1/22 (4.6%) females were positive, no statistically significant association between sex and hemoplasma infection was found (P = 0.19). Viral quantitative PCR (qPCR) was performed on 43/45 samples, among which 2/43 (4.7%) were positive for FIV and none for FeLV. Only one cat (2.3%) was coinfected with hemoplasma and FIV (P = 0.26). In addition, 4/6 (66.7%) cats that tested positive for hemoplasmas were infested by fleas (P = 0.0014) and/or ticks (P = 0.25). These results show that even if the free-roaming cat population is clinically healthy and has adequate access to food, it may present flea infestation and hemoplasma infection with lower packed cell volume values.
Keywords	Feline immunodeficiency virus ; Feline leukemia virus ; Mycoplasma haemofelis ; Mycoplasma haemominutum ; Siphonaptera ; blood ; cats ; hematocrit ; medicine ; mixed infection ; parasitism ; quantitative polymerase chain reaction ; surgery ; ticks ; Brazil ; Animal health ; coinfection ; hemotropic mycoplasma ; molecular diagnosis
Language	English
Dates of publication	2023-02
Size	p. 20551169221148672
Publishing place	SAGE Publications
Document type	Article ; Online
ZDB-ID	2822177-1
ISSN	2055-1169
ISSN	2055-1169
DOI	10.1177/20551169221148672
Database	NAL-Catalogue (AGRICOLA)

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Article: Molecular Investigation Confirms

Mathias, Bruno da Silva / Minozzo, Guilherme Augusto / Biondo, Alexander Welker / Costa, Jaciara de Oliveira Jorge / Soares, Herbert Sousa / Marcili, Arlei / Guimarães, Lilian de Oliveira / Anjos, Carolina Clares Dos / Santos, Andrea Pires Dos / Riediger, Irina Nastassja / Fecchio, Alan / Bueno, Marina Galvão / Pinho, João Batista / Kirchgatter, Karin

Microorganisms

2023 Volume 11, Issue 6

Abstract: ... ...

Abstract	Plasmodium
Language	English
Publishing date	2023-06-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms11061531
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Serosurvey of anti-treponema pallidum (syphilis), anti-hepatitis C virus and anti-HIV antibodies in homeless persons of São Paulo city, southeastern Brazil.

Felipetto, Laís Giuliani / Teider-Junior, Pedro Irineu / da Silva, Felipe Fortino Verdan / Couto, Anahi Chechia do / Kmetiuk, Louise Bach / Martins, Camila Marinelli / Ullmann, Leila Sabrina / Timenetsky, Jorge / Santos, Andrea Pires Dos / Biondo, Alexander Welker

The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases

2021 Volume 25, Issue 4, Page(s) 101602

Abstract: Homeless persons have been considered as one of the most susceptible populations to sexually transmitted infections. In Brazil, these population experienced an increase of 140% from 2012 to 2020. Accordingly, the present study aimed to assess the ... ...

Abstract	Homeless persons have been considered as one of the most susceptible populations to sexually transmitted infections. In Brazil, these population experienced an increase of 140% from 2012 to 2020. Accordingly, the present study aimed to assess the seroprevalence of anti-Treponema pallidum, anti-HCV, anti-HIV antibodies, and the risk factors associated with homeless persons in a daytime attendance shelter of São Paulo city during the syphilis epidemic in Brazil. Blood samples of 116 volunteers and epidemiological data were conveniently collected in the shelter from June through August 2018. Detection of syphilis, HCV, and HIV antibodies was performed by chemiluminescent microparticle immunoassay (CMIA). CMIA-reagent samples for anti-T. pallidum antibodies were confirmed by Venereal Disease Research Laboratory (VDRL) non-treponemal test. VDRL non-reagent samples were confirmed by treponemal rapid immunochromatographic test. A rapid immunoblot assay confirmed seropositivity to HIV. Overall, anti-T. pallidum antibodies were observed in 29/116 (25.0%), anti-HCV antibodies in 4/116 (3.4%), and anti-HIV antibodies in 2/116 (1.7%) individuals, both co-infected with anti-T. pallidum antibodies. Associated risk factors for syphilis in homeless persons were being born or previously living in another city (p = 0.043) and becoming homeless due to family conflicts (p = 0.035). Besides homeless vulnerability, worldwide shortage of benzathine penicillin supply and increasing of syphilis testing access through rapid testing in primary health care services may have also impacted disease spreading at the time. The prevalence of syphilis found herein is the highest worldwide to date in this population.
MeSH term(s)	Brazil/epidemiology ; HIV Antibodies ; HIV Infections/epidemiology ; HIV-1 ; Hepacivirus ; Hepatitis C/epidemiology ; Homeless Persons ; Humans ; Seroepidemiologic Studies ; Syphilis/epidemiology ; Treponema pallidum
Chemical Substances	HIV Antibodies
Language	English
Publishing date	2021-08-10
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2041400-6
ISSN	1678-4391 ; 1413-8670
ISSN (online)	1678-4391
ISSN	1413-8670
DOI	10.1016/j.bjid.2021.101602
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 5633: Show issues

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Article ; Online: Serosurvey of Rickettsia spp. in cats from a Brazilian spotted fever-endemic area.

Mendes, Juliana Cristina Rebonato / Kmetiuk, Louise Bach / Martins, Camila Marinelli / Canavessi, Aurea Maria Oliveira / Jimenez, Tatiana / Pellizzaro, Maysa / Martins, Thiago Fernandes / Morikawa, Vivien Midori / Santos, Andrea Pires Dos / Labruna, Marcelo Bahia / Biondo, Alexander Welker

Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria

2019 Volume 28, Issue 4, Page(s) 713–721

Abstract: Rickettsia spp. bacteria are responsible for tick-borne diseases worldwide, mostly maintained by rickettsial amplifiers capybaras in Brazilian endemic areas. The campus of the University of São Paulo, in southeastern Brazil, is an area endemic for ... ...

Abstract	Rickettsia spp. bacteria are responsible for tick-borne diseases worldwide, mostly maintained by rickettsial amplifiers capybaras in Brazilian endemic areas. The campus of the University of São Paulo, in southeastern Brazil, is an area endemic for Brazilian spotted fever (BSF), with high density of capybaras and Amblyomma spp., along with confirmed human cases. Besides capybaras, the university has also an in-campus high population of sheltered and free-roaming cats. Accordingly, the aim of this study was to determine the prevalence and characteristics associated with Rickettsia rickettsii, Rickettsia parkeri and Rickettsia felis exposure among cats in a BSF-endemic area. Out of 51 cats sampled, 23/35 shelter (65.7%) and 5/16 free-roaming (31.2%) were positive (titers ≥ 64) for at least one Rickettsia species. Ticks species were present in 3/16 free-roaming cats (18.8%), consisting of Amblyomma spp., nymphs of Amblyomma sculptum and adult Rhipicephalus sanguineus sensu lato. Despite sharing the capybaras environment, the seropositivity among the free-roaming and shelter cats was lower than owned cats in other endemic areas. Whether equally or less exposed to rickettsial infection, compared with owned cats in endemic areas, free-roaming and shelter cats may be used as environmental sentinels for human exposure to rickettsiae in such areas.
MeSH term(s)	Animals ; Brazil/epidemiology ; Cat Diseases/diagnosis ; Cat Diseases/epidemiology ; Cat Diseases/microbiology ; Cats ; Female ; Male ; Rickettsia/classification ; Rickettsia/isolation & purification ; Rickettsia Infections/diagnosis ; Rickettsia Infections/epidemiology ; Rickettsia Infections/microbiology ; Rickettsia Infections/veterinary ; Rocky Mountain Spotted Fever/transmission ; Ticks/microbiology
Language	English
Publishing date	2019-11-13
Publishing country	Brazil
Document type	Journal Article
ISSN	1984-2961
ISSN (online)	1984-2961
DOI	10.1590/S1984-29612019092
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Molecular detection of Leptospira spp. in rats as early spatial predictor for human disease in an endemic urban area.

Pellizzaro, Maysa / Martins, Camila Marinelli / Yamakawa, Ana Carolina / Ferraz, Diogo da Cunha / Morikawa, Vivien Midori / Ferreira, Fernando / Santos, Andrea Pires Dos / Biondo, Alexander Welker / Langoni, Helio

PloS one

2019 Volume 14, Issue 5, Page(s) e0216830

Abstract: Background: Leptospirosis is considered a neglected zoonosis associated with infrastructure problems and low socioeconomic status, particularly slums. Since the disease is mainly transmitted in urban settings by rat urine, this risk factor may be ... ...

Abstract	Background: Leptospirosis is considered a neglected zoonosis associated with infrastructure problems and low socioeconomic status, particularly slums. Since the disease is mainly transmitted in urban settings by rat urine, this risk factor may be important predictor tool for prompt control and effective prevention at the local level in urban endemic areas. Accordingly, the present study aimed to propose an early spatial predictor tool for human leptospirosis in urban settings, to test the methodology of molecular methods for assessing Leptospira spp. in trapped rats, and report associated environmental data. Methodology/principal findings: Official city records and previous study were used to select risk factors for human leptospirosis in an endemic neighborhood of Curitiba, Brazil. Neighborhood census sectors were divided in high- and low-risk areas using 12 selected factors: flood area, water supply, water course, green coverage, afforestation, sewage network, open sewage, open garbage, garbage collection, dumpster, pavement, and rodent complaints. In addition, rats were captured in pre-determined sites from January through March 2017, euthanized, and individual kidneys samples sent for molecular diagnosis. Human cases were obtained from official city records. In total, 95/112 (84.8%) census sectors were classified as low-risk to human leptospirosis. No significant statistical differences were found in human case frequencies between high and low-risk areas. Kidney samples from 17/25 (68.0%) trapped rats were positive for Leptospira spp. The main risk factors associated with rodent presence included inadequate water supply (p = 0.04), sanitary sewage (p = 0.04), unpaved streets (p = 0.04), and complaint of rodents (p = 0.04). Conclusions/significance: This study offers a new approach to score leptospirosis transmission risk, and to compare small areas and their heterogeneity in the same census sector of endemic areas. Environmental risk factors for Leptospira spp. transmission within the neighborhood were mainly due to differences in infrastructure and basic services. To the author's knowledge, this is the first study using Leptospira spp. in rats as predictor for human disease in an urban setting of a major city. Although the number of rats trapped was low, this methodology may be used as basis for early and effective interventions, focused on high risk areas for leptospirosis prior to human cases, and potentially reducing morbidity and mortality in low-income areas of urban settings.
MeSH term(s)	Animals ; Brazil ; Cities ; Humans ; Leptospira/genetics ; Leptospirosis/diagnosis ; Leptospirosis/epidemiology ; Leptospirosis/genetics ; Pathology, Molecular ; Poverty Areas ; Rats ; Risk Factors ; Zoonoses/diagnosis ; Zoonoses/epidemiology ; Zoonoses/genetics
Language	English
Publishing date	2019-05-22
Publishing country	United States
Document type	Journal Article
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0216830
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Hemoplasma prevalence and hematological abnormalities associated with infection in three different cat populations from Southern Brazil.

Santos, Andrea Pires dos / Conrado, Francisco de Oliveira / Messick, Joanne Belle / Biondo, Alexander Welker / Oliveira, Simone Tostes de / Guimaraes, Ana Marcia Sá / Nascimento, Naíla Cannes do / Pedralli, Viviane / Lasta, Camila Serina / González, Félix Hilário Diaz

Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria

2014 Volume 23, Issue 4, Page(s) 428–434

Abstract: Three hemoplasma species are recognized in domestic cats: Mycoplasma haemofelis, 'Candidatus Mycoplasma haemominutum' and 'Candidatus Mycoplasma turicensis'. We report the prevalence and hematological abnormalities of hemoplasma infection in 369 domestic ...

Abstract	Three hemoplasma species are recognized in domestic cats: Mycoplasma haemofelis, 'Candidatus Mycoplasma haemominutum' and 'Candidatus Mycoplasma turicensis'. We report the prevalence and hematological abnormalities of hemoplasma infection in 369 domestic cats from three different populations (blood donors, hospitalized cats and shelter cats) from Southern Brazil. Complete blood counts were performed at the time of blood collection, and DNA was extracted and tested by conventional PCR for each hemoplasma species. A total of 79 samples (21.40%) were positive for at least one species. The most prevalent hemoplasma was 'Candidatus Mycoplasma haemominutum', with 50/369 (13.55%) positive cats, followed by 'Candidatus Mycoplasma turicensis', 10/369 (2.71%), and Mycoplasma haemofelis, 8/369 (2.16%). Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' coinfection was observed in 4/369 (1.08%), whereas 'Candidatus Mycoplasma haemominutum' and 'Candidatus Mycoplasma turicensis' in 5/369 (1.35%). Three cats (0.81%) were infected with all three hemoplasmas. There was no association between infection and the different populations. Anemia was associated with Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum', but not with 'Candidatus Mycoplasma turicensis'. Male cats and cats with outdoor access were more likely to be infected. Although 'Candidatus Mycoplasma haemominutum' is believed to cause minimal or no hematological alterations, the infected cats studied herein were more likely to be anemic.
MeSH term(s)	Anemia/epidemiology ; Anemia/veterinary ; Animals ; Brazil/epidemiology ; Cat Diseases/epidemiology ; Cat Diseases/microbiology ; Cats ; Female ; Hematologic Diseases/epidemiology ; Hematologic Diseases/veterinary ; Male ; Mycoplasma Infections/epidemiology ; Mycoplasma Infections/veterinary ; Prevalence
Language	English
Publishing date	2014-10
Publishing country	Brazil
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1984-2961
ISSN (online)	1984-2961
DOI	10.1590/S1984-29612014079
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: MOLECULAR INVESTIGATION OF HEMOTROPIC MYCOPLASMAS IN HUMAN BEINGS, DOGS AND HORSES IN A RURAL SETTLEMENT IN SOUTHERN BRAZIL.

Vieira, Rafael Felipe da Costa / Vidotto, Odilon / Vieira, Thállitha Samih Wischral Jayme / Guimaraes, Ana Márcia Sá / Santos, Andrea Pires dos / Nascimento, Naíla Cannes / Santos, Nelson Jesse Rodrigues dos / Martins, Thiago Fernandes / Labruna, Marcelo Bahia / Marcondes, Mary / Biondo, Alexander Welker / Messick, Joanne Belle

Revista do Instituto de Medicina Tropical de Sao Paulo

2015 Volume 57, Issue 4, Page(s) 353–357

Abstract: The aims of this study were to determine the prevalence of hemoplasmas in a rural Brazilian settlement's population of human beings, their dogs and horses, highly exposed to tick bites; to identify the tick species parasitizing dogs and horses, and ... ...

Abstract	The aims of this study were to determine the prevalence of hemoplasmas in a rural Brazilian settlement's population of human beings, their dogs and horses, highly exposed to tick bites; to identify the tick species parasitizing dogs and horses, and analyze factors associated with their infection. Blood samples from 132 dogs, 16 horses and 100 humans were screened using a pan-hemoplasma SYBR green real-time PCR assay followed by a species-specific TaqMan real-time PCR. A total of 59/132 (44.7%) dog samples were positive for hemoplasmas (21 Mycoplasma haemocanis alone, 12 ' Candidatus Mycoplasma haematoparvum' alone and 21 both). Only 1/100 (1.0%) human sample was positive by qPCR SYBR green, with no successful amplification of 16S rRNA or 23 rRNA genes despite multiple attempts. All horse samples were negative. Dogs >1 year of age were more likely to be positive for hemoplasmas ( p= 0.0014). In conclusion, although canine hemoplasma infection was highly prevalent, cross-species hemoplasma transmission was not observed, and therefore may not frequently occur despite overexposure of agents and vectors.
MeSH term(s)	Animals ; Brazil/epidemiology ; Dog Diseases/epidemiology ; Dog Diseases/microbiology ; Dogs ; Female ; Horse Diseases/epidemiology ; Horse Diseases/microbiology ; Horses ; Humans ; Mycoplasma/classification ; Mycoplasma/genetics ; Mycoplasma Infections/epidemiology ; Mycoplasma Infections/microbiology ; Mycoplasma Infections/veterinary ; Phylogeny ; Prevalence ; Real-Time Polymerase Chain Reaction ; Risk Factors ; Rural Population
Language	English
Publishing date	2015-07
Publishing country	Brazil
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	128928-7
ISSN	1678-9946 ; 0036-4665
ISSN (online)	1678-9946
ISSN	0036-4665
DOI	10.1590/S0036-46652015000400014
Database	MEDical Literature Analysis and Retrieval System OnLINE

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