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  1. Book: Microbial metabolic engineering

    Santos, Christine Nicole S. / Ajikumar, Parayil Kumaran

    methods and protocols

    (Methods in molecular biology ; 1927 ; Springer protocols)

    2019  

    Author's details edited by Christine Nicole S. Santos and Parayil Kumaran Ajikumar
    Series title Methods in molecular biology ; 1927
    Springer protocols
    Collection
    Language English
    Size x, 252 Seiten, Illustrationen
    Publisher Humana Press
    Publishing place New York, NY
    Publishing country United States
    Document type Book
    HBZ-ID HT019995897
    ISBN 978-1-4939-9141-9 ; 9781493991426 ; 1-4939-9141-8 ; 1493991426
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 7042 -1927- verfügbar in Königswinter Königswinter

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  2. Article ; Online: Editorial overview: nature's catalysts: The important role of enzymes in biotechnology.

    Parayil, Ajikumar / Santos, Christine

    Current opinion in biotechnology

    2021  Volume 69, Page(s) iii–v

    MeSH term(s) Biotechnology ; Catalysis
    Language English
    Publishing date 2021-06-27
    Publishing country England
    Document type Editorial
    ZDB-ID 1052045-4
    ISSN 1879-0429 ; 0958-1669
    ISSN (online) 1879-0429
    ISSN 0958-1669
    DOI 10.1016/j.copbio.2021.06.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Frontal Arteriovenous Malformation Presenting as Painful Unilateral Conjunctiva Injection.

    Harkins, Andrew / Bassig-Santos, Christine / Cirone, Michael

    Clinical practice and cases in emergency medicine

    2022  Volume 6, Issue 1, Page(s) 96–98

    Abstract: Case presentation: Arteriovenous malformations (AVM) have a variety of clinically significant manifestations. This report details a patient who presented with unilateral conjunctiva injection, which was found to be due to an atypical manifestation of an ...

    Abstract Case presentation: Arteriovenous malformations (AVM) have a variety of clinically significant manifestations. This report details a patient who presented with unilateral conjunctiva injection, which was found to be due to an atypical manifestation of an AVM with a large draining vein mimicking carotid cavernous fistula.
    Discussion: While imaging for patients presenting with eye pain and unilateral conjunctiva injection is not always warranted, emergency physicians should keep their differential diagnosis broad and pursue additional workup when warning signs of more sinister pathology present.
    Language English
    Publishing date 2022-02-28
    Publishing country United States
    Document type Journal Article
    ISSN 2474-252X
    ISSN (online) 2474-252X
    DOI 10.5811/cpcem.2021.10.54268
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Refusal to Bear Weight in a Previously Healthy 8-year-old Girl.

    Sherwood, Karla / Santos, Christine / Rao, Latha / Webb, Nicole

    Pediatrics in review

    2022  Volume 42, Issue 12, Page(s) 698–701

    MeSH term(s) Child ; Female ; Health Status ; Humans ; Weight-Bearing
    Language English
    Publishing date 2022-08-26
    Publishing country United States
    Document type Case Reports ; Journal Article
    ZDB-ID 774515-1
    ISSN 1526-3347 ; 0191-9601
    ISSN (online) 1526-3347
    ISSN 0191-9601
    DOI 10.1542/pir.2020-0085
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  5. Article ; Online: High-Altitude Pulmonary Edema in Mountain Community Residents.

    Ebert-Santos, Christine

    High altitude medicine & biology

    2017  Volume 18, Issue 3, Page(s) 278–284

    Abstract: Ebert-Santos, Christine. High-altitude pulmonary edema in mountain community residents. High Alt Med Biol. 18:278-284, 2017.-High-altitude pulmonary edema (HAPE) affects lowlanders ascending quickly to elevations above 2440 m. Mountain resident children ... ...

    Abstract Ebert-Santos, Christine. High-altitude pulmonary edema in mountain community residents. High Alt Med Biol. 18:278-284, 2017.-High-altitude pulmonary edema (HAPE) affects lowlanders ascending quickly to elevations above 2440 m. Mountain resident children with no travel can sometimes develop HAPE as was observed over 30 years ago (Fasules et al., 1985). This is not well known and children instead are diagnosed as having pneumonia or asthma. In our clinic at 2800 m, we see children presenting with severe hypoxemia, clinical, and radiographic findings consistent with HAPE despite no recent travel. We call this mountain resident HAPE. We reviewed records of 48 patients with pulmonary symptoms. Analysis included vital signs, pulse oximetry, laboratories, physical findings, and clinical course. We identified 33 residents with HAPE and no travel, five with reentry HAPE, two visitors with classic HAPE, six residents with pneumonia, and two with asthma. Also, 48 X-rays on hypoxemic children seen between 2006 and 2017 were reviewed. Five showed definite HAPE with follow-up X-rays within 48 hours confirming rapid clearing on oxygen, 27 showed findings consistent with HAPE or viral pneumonia and no repeat study. Children living at elevation presenting with hypoxemia are commonly misdiagnosed. Rapid improvement with oxygen and little to no improvement with bronchodilators are more consistent with HAPE, and thus, antibiotics and other treatments can be avoided.
    Language English
    Publishing date 2017-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2076262-8
    ISSN 1557-8682 ; 1527-0297
    ISSN (online) 1557-8682
    ISSN 1527-0297
    DOI 10.1089/ham.2016.0100
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Targeted Mass Spectrometry-Based Proteomics Tools for Strain Optimization.

    Tseng, Hsien-Chung / Santos, Christine Nicole S

    Methods in molecular biology (Clifton, N.J.)

    2019  Volume 1927, Page(s) 191–201

    Abstract: The goal of strain optimization is to create high-performance strains producing compounds of interest at a high titer, yield, and volumetric productivity. The effectiveness of strain optimization relies on methodologies used to aid optimization of native ...

    Abstract The goal of strain optimization is to create high-performance strains producing compounds of interest at a high titer, yield, and volumetric productivity. The effectiveness of strain optimization relies on methodologies used to aid optimization of native or novel pathways within cells. Many different factors, including mRNA abundance, protein abundance, and enzyme activity/stability, will contribute to the strain performance, which is not often evident by simply monitoring product titers. To this end, targeted proteomics tools utilizing LC-MS-MS (liquid chromatography coupled with tandem mass spectrometry) have recently been developed and can monitor protein levels at great sensitivities. Here, we describe all relevant aspects when developing proteomics tools for strain optimization.
    MeSH term(s) Biotechnology ; Chromatography, High Pressure Liquid ; Chromatography, Liquid ; Data Analysis ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Metabolic Engineering ; Proteomics/methods ; Tandem Mass Spectrometry
    Language English
    Publishing date 2019-02-20
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-9142-6_13
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Balancing glucose and oxygen uptake rates to enable high amorpha-4,11-diene production in Escherichia coli via the methylerythritol phosphate pathway.

    Patil, Vikas / Santos, Christine N S / Ajikumar, Parayil K / Sarria, Stephen / Takors, Ralf

    Biotechnology and bioengineering

    2021  Volume 118, Issue 3, Page(s) 1317–1329

    Abstract: Amorpha-4,11-diene (AMD4,11) is a precursor to artemisinin, a potent antimalarial drug that is traditionally extracted from the shrubs of Artemisia annua. Despite significant prior efforts to produce artemisinin and its precursors through biotechnology, ... ...

    Abstract Amorpha-4,11-diene (AMD4,11) is a precursor to artemisinin, a potent antimalarial drug that is traditionally extracted from the shrubs of Artemisia annua. Despite significant prior efforts to produce artemisinin and its precursors through biotechnology, there remains a dire need for more efficient biosynthetic routes for its production. Here, we describe the optimization of key process conditions for an Escherichia coli strain producing AMD4,11 via the native methylerythritol phosphate (MEP) pathway. By studying the interplay between glucose uptake rates and oxygen demand, we were able to identify optimal conditions for increasing carbon flux through the MEP pathway by manipulating the availability of NADPH required for terpenoid production. Installation of an optimal q
    MeSH term(s) Antimalarials/metabolism ; Erythritol/analogs & derivatives ; Erythritol/metabolism ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Glucose/metabolism ; Oxygen/metabolism ; Oxygen Consumption ; Polycyclic Sesquiterpenes/metabolism ; Sugar Phosphates/metabolism
    Chemical Substances 2-C-methylerythritol 4-phosphate ; Antimalarials ; Polycyclic Sesquiterpenes ; Sugar Phosphates ; amorpha-4,11-diene ; Glucose (IY9XDZ35W2) ; Erythritol (RA96B954X6) ; Oxygen (S88TT14065)
    Language English
    Publishing date 2021-01-15
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 280318-5
    ISSN 1097-0290 ; 0006-3592
    ISSN (online) 1097-0290
    ISSN 0006-3592
    DOI 10.1002/bit.27655
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Pediatric Otoscopy Video Screening With Shift Contrastive Anomaly Detection.

    Wang, Weiyao / Tamhane, Aniruddha / Santos, Christine / Rzasa, John R / Clark, James H / Canares, Therese L / Unberath, Mathias

    Frontiers in digital health

    2022  Volume 3, Page(s) 810427

    Abstract: Ear related concerns and symptoms represent the leading indication for seeking pediatric healthcare attention. Despite the high incidence of such encounters, the diagnostic process of commonly encountered diseases of the middle and external presents a ... ...

    Abstract Ear related concerns and symptoms represent the leading indication for seeking pediatric healthcare attention. Despite the high incidence of such encounters, the diagnostic process of commonly encountered diseases of the middle and external presents a significant challenge. Much of this challenge stems from the lack of cost effective diagnostic testing, which necessitates the presence or absence of ear pathology to be determined clinically. Research has, however, demonstrated considerable variation among clinicians in their ability to accurately diagnose and consequently manage ear pathology. With recent advances in computer vision and machine learning, there is an increasing interest in helping clinicians to accurately diagnose middle and external ear pathology with computer-aided systems. It has been shown that AI has the capacity to analyze a single clinical image captured during the examination of the ear canal and eardrum from which it can determine the likelihood of a pathognomonic pattern for a specific diagnosis being present. The capture of such an image can, however, be challenging especially to inexperienced clinicians. To help mitigate this technical challenge, we have developed and tested a method using video sequences. The videos were collected using a commercially available otoscope smartphone attachment in an urban, tertiary-care pediatric emergency department. We present a two stage method that first, identifies valid frames by detecting and extracting ear drum patches from the video sequence, and second, performs the proposed shift contrastive anomaly detection (SCAD) to flag the otoscopy video sequences as normal or abnormal. Our method achieves an AUROC of 88.0% on the patient level and also outperforms the average of a group of 25 clinicians in a comparative study, which is the largest of such published to date. We conclude that the presented method achieves a promising first step toward the automated analysis of otoscopy video.
    Language English
    Publishing date 2022-02-10
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2673-253X
    ISSN (online) 2673-253X
    DOI 10.3389/fdgth.2021.810427
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Engineering complex biological systems in bacteria through recombinase-assisted genome engineering.

    Santos, Christine Nicole S / Yoshikuni, Yasuo

    Nature protocols

    2014  Volume 9, Issue 6, Page(s) 1320–1336

    Abstract: Here we describe an advanced paradigm for the design, construction and stable implementation of complex biological systems in microbial organisms. This engineering strategy was previously applied to the development of an Escherichia coli-based platform, ... ...

    Abstract Here we describe an advanced paradigm for the design, construction and stable implementation of complex biological systems in microbial organisms. This engineering strategy was previously applied to the development of an Escherichia coli-based platform, which enabled the use of brown macroalgae as a feedstock for the production of biofuels and renewable chemicals. In this approach, functional genetic modules are first designed in silico and constructed on a bacterial artificial chromosome (BAC) by using a recombineering-based inchworm extension technique. Stable integration into the recipient chromosome is then mediated through the use of recombinase-assisted genome engineering (RAGE). The flexibility, simplicity and speed of this method enable a comprehensive optimization of several different parameters, including module configuration, strain background, integration locus, gene copy number and intermodule compatibility. This paradigm therefore has the potential to markedly expedite most strain-engineering endeavors. Once a biological system has been designed and constructed on a BAC, its implementation and optimization in a recipient host can be carried out in as little as 1 week.
    MeSH term(s) Bacteria/genetics ; Chromosomes, Artificial, Bacterial/genetics ; Genetic Engineering/methods ; Genome, Bacterial/genetics ; Metabolic Networks and Pathways/genetics ; Recombinases/metabolism
    Chemical Substances Recombinases
    Language English
    Publishing date 2014-05-15
    Publishing country England
    Document type Journal Article
    ZDB-ID 2244966-8
    ISSN 1750-2799 ; 1754-2189
    ISSN (online) 1750-2799
    ISSN 1754-2189
    DOI 10.1038/nprot.2014.084
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  10. Article ; Online: How do we operate a large monthly red blood cell exchange program.

    Allison, David / Manon, Luis / Vidanovic, Vladimir / Crane, Jason / Sevcik, Joan / Janiszewski, Sara / Gabert, Kimberly / Santos, Christine Clemente Dos / Discua, Joseph / Hsu, Lewis L / Gordeuk, Victor / Campbell-Lee, Sally

    Transfusion

    2023  Volume 63, Issue 3, Page(s) 450–456

    Abstract: Background: Red blood cell (RBC) exchange for sickle cell disease presents unique difficulties due to RBC phenotyping, complex antibody work-ups, large number of RBC units required, and vascular access considerations, any of which can delay the ... ...

    Abstract Background: Red blood cell (RBC) exchange for sickle cell disease presents unique difficulties due to RBC phenotyping, complex antibody work-ups, large number of RBC units required, and vascular access considerations, any of which can delay the procedure. Multidisciplinary coordination and systemic processes ensure that monthly appointments remain on schedule.
    Study design and methods: A high-volume chronic RBC exchange program is described, highlighting the importance of multidisciplinary coordination and process improvement strategies involving initial referral, vascular access, order sets, and allocation of antigen-negative or phenotypically matched RBCs.
    Results: Approximately 50 outpatient RBC exchanges are performed each month with an 82% kept-appointment rate. Specific factors for program success include open communication across services and improvements to referrals and standardized order sets.
    Conclusion: A combination of multidisciplinary coordination and process improvement can ensure the success of a high volume RBC exchange program. Frequent communication of upcoming appointments between the referring hematologists, the hemapheresis clinic, transfusion service, and interventional radiology is critical. Advance notice to the immunohematology reference lab of upcoming appointments is needed to allow enough time for allocating antigen-negative RBCs. Order sets can be leveraged to standardize and streamline RBC exchanges. Lastly, numerous mechanisms help patients compensate for the cognitive sequelae of stroke.
    MeSH term(s) Humans ; Erythrocyte Transfusion/methods ; Anemia, Sickle Cell ; Erythrocytes ; Blood Component Removal ; Stroke
    Language English
    Publishing date 2023-01-11
    Publishing country United States
    Document type Case Reports
    ZDB-ID 208417-x
    ISSN 1537-2995 ; 0041-1132
    ISSN (online) 1537-2995
    ISSN 0041-1132
    DOI 10.1111/trf.17245
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