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  1. Article: DNA metabarcoding assessment of Neotropical ichthyoplankton communities is marker-dependent.

    Teixeira, Daniel Fonseca / Hilário, Heron Oliveira / Santos, Gilmar Bastos / Carvalho, Daniel Cardoso

    Ecology and evolution

    2023  Volume 13, Issue 10, Page(s) e10649

    Abstract: The study of ichthyoplankton is paramount to understanding fish assemblages' reproductive dynamics. DNA metabarcoding has been applied as a rapid, cost-effective, and accurate taxonomy tool, allowing the identification of multiple individuals ... ...

    Abstract The study of ichthyoplankton is paramount to understanding fish assemblages' reproductive dynamics. DNA metabarcoding has been applied as a rapid, cost-effective, and accurate taxonomy tool, allowing the identification of multiple individuals simultaneously. However, there remain significant challenges when using DNA metabarcoding, such as molecular marker choice according to the taxonomic resolution and length of the fragment to be sequenced, primer bias, incomplete reference databases, and qualitative inference incongruences. Here, 30 ichthyoplankton pools collected from a Neotropical river were identified at a molecular level using DNA metabarcoding to compare the resolution, sensibility, specificity, and relative read abundance (RRA) recovery of three molecular markers: the standard COI fragment (650 pb, with each end analyzed individually) and two short 12S rRNA genes markers (≅200 bp - NeoFish and MiFish markers). The combined use of the three markers increased the genera detection rates by 25%-87.5%, allowing an increased taxonomic coverage and robust taxonomic identification of complex Neotropical ichthyoplankton communities. RRA is marker-dependent, indicating caution is still needed while inferring species abundance based on DNA metabarcoding data when using PCR-dependent protocols.
    Language English
    Publishing date 2023-10-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 2635675-2
    ISSN 2045-7758
    ISSN 2045-7758
    DOI 10.1002/ece3.10649
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Fish‐based multimetric index for evaluating land use effects on large neotropical reservoirs

    de Oliveira Sanches, Bárbara / Becker, Bárbara / Hughes, Robert Mason / Petesse, Maria Letizia / Ribeiro, Julia Ramos / Santos, Gilmar Bastos

    Journal of applied ichthyology. 2019 Oct., v. 35, no. 5

    2019  

    Abstract: Because of their importance to human well‐being and ecosystem services, it is important to have cost‐effective methods for assessing reservoir ecological condition or health. To do so, we constructed a preliminary reservoir fish assemblage index (RFAI) ... ...

    Abstract Because of their importance to human well‐being and ecosystem services, it is important to have cost‐effective methods for assessing reservoir ecological condition or health. To do so, we constructed a preliminary reservoir fish assemblage index (RFAI) to assess the effect of predominantly agricultural land use on two oligotrophic neotropical reservoirs that differed in disturbance level. We first determined the disturbance levels around each reservoir through use of an integrated disturbance index (IDI). The IDI was calculated from the combination of land use metrics at local (Local Disturbance Index ‐ LDI) and buffer scales (Buffer Disturbance Index ‐ BDI). Afterwards, we employed the IDI to assess the response of candidate metrics to environmental disturbance in the RFAI construction. The final RFAI included three metrics selected by range, responsiveness, and redundancy tests. Metrics were scored continuously, and the final RFAI was divided in four quality classes: very poor, poor, acceptable and good. The results showed a clear disturbance gradient in both reservoirs with the IDI values varying between 0.07 and 1.33. However, the IDI values were greater around Volta Grande reservoir because of the predominance of agriculture land use (at local and buffer scales). The RFAI scores in Nova Ponte reservoir were significantly higher than in Volta Grande. All the sites scored as very poor or poor were in Volta Grande and all the sites scored as good were in Nova Ponte. In addition, RFAI scores in the reservoir river arms did not differ from those in the reservoir main body, indicating that it was applicable to both habitat types. Finally, our RFAI scores indicated no significant seasonal difference in the two reservoirs; however, the rainy season produced a greater range and more low scores, particularly in Volta Grande. We related this result to the harmful effect of agriculture and the surface runoff of fertilizers, herbicides, and pesticides. Thus, the RFAI was effective in detecting agricultural impacts at the site scale, even in oligotrophic reservoirs.
    Keywords agricultural land ; cost effectiveness ; ecosystem services ; fertilizers ; fish ; habitats ; herbicides ; land use ; Neotropics ; rivers ; runoff ; seasonal variation ; social welfare ; water reservoirs ; wet season
    Language English
    Dates of publication 2019-10
    Size p. 1129-1140.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 283875-8
    ISSN 0175-8659
    ISSN 0175-8659
    DOI 10.1111/jai.13954
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Length–weight relationships of 78 fish species from São Francisco River basin, Brazil

    Pessali, Tiago Casarim / Salvador, Gilberto Nepomuceno / Alves, Carlos Bernardo Mascarenhas / Drager, Dennys Heilbuth Cachapuz / Zawadzki, Cláudio Henrique / Prata, Erival Gonçalves / Vieira, Fábio / Santos, Gilmar Bastos / Rosa, Gustavo Ribeiro / Penido, Iago de Souza / Gomes, João Pedro Corrêa / Brito, Marcelo Fulgencio Guedes / Pompeu, Paulo Santos / Leitão, Rafael Pereira / dos Santos, Sérgio Alexandre / Barroso, Tobias Antônio / Ribeiro, Thiago Cotta / Sousa, Tarcísio José / Dala‐Corte, Renato Bolson /
    Freitas, Tiago Magalhães da Silva

    Journal of applied ichthyology. 2022 May, v. 38, no. 3

    2022  

    Abstract: We established the length–weight relationships (LWR) for 78 fish species from the São Francisco River basin, Brazil. Samples were originated from different scientific projects performed between 1993 and 2019. Fishing gears used were mainly gillnets and ... ...

    Abstract We established the length–weight relationships (LWR) for 78 fish species from the São Francisco River basin, Brazil. Samples were originated from different scientific projects performed between 1993 and 2019. Fishing gears used were mainly gillnets and cast nets of various mesh sizes. Specimens varied from 0.6 to 128 cm (18.1 ± 11.8 cm) and from 0.01 to 26,900 g (317.9 ± 733.6 g). The allometric coefficient b varied from 2.509 to 3.494. For 66 species, the LWR parameters were completely novel and, for the other 12 species, are present for the first time for the São Francisco River basin.
    Keywords allometry ; fish ; gillnets ; ichthyology ; watersheds ; Brazil
    Language English
    Dates of publication 2022-05
    Size p. 344-351.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 283875-8
    ISSN 0175-8659
    ISSN 0175-8659
    DOI 10.1111/jai.14312
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: Genetic evaluation of migratory fish: Implications for conservation and stocking programs.

    Pimentel, Juliana da Silva Martins / Ludwig, Sandra / Resende, Leonardo Cardoso / Brandão-Dias, Pedro Ferreira Pinto / Pereira, Adriana Heloísa / de Abreu, Nazaré Lúcio / Rosse, Izinara Cruz / Martins, Ana Paula Vimieiro / Facchin, Susanne / Lopes, João de Magalhães / Santos, Gilmar Bastos / Alves, Carlos Bernado Mascarenhas / Kalapothakis, Evanguedes

    Ecology and evolution

    2020  Volume 10, Issue 19, Page(s) 10314–10324

    Abstract: Fish stocking programs have been implemented to mitigate the blockage of original riverbeds by the construction of hydropower dams, which affects the natural migration of fish populations. However, this method raises concerns regarding the genetic rescue ...

    Abstract Fish stocking programs have been implemented to mitigate the blockage of original riverbeds by the construction of hydropower dams, which affects the natural migration of fish populations. However, this method raises concerns regarding the genetic rescue of the original populations of migratory fish species. We investigated the spatial distribution of genetic properties, such as genetic diversity, population structure, and gene flow (migration), of the Neotropical migratory fish
    Language English
    Publishing date 2020-09-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 2635675-2
    ISSN 2045-7758
    ISSN 2045-7758
    DOI 10.1002/ece3.6231
    Database MEDical Literature Analysis and Retrieval System OnLINE

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