Article ; Online: Combining diagnostic procedures for the management of leishmaniasis in areas with high prevalence of Leishmania guyanensis.
Anais brasileiros de dermatologia
2012 Volume 86, Issue 6, Page(s) 1141–1144
Abstract: Background: The Amazon region corresponds to approximately 40% of the cases of leishmaniasis in Brazil. We report a prospective study with 180 patients conducted in a health care unit that diagnoses 10% of the cases of leishmaniasis in the Brazilian ... ...
| Abstract | Background: The Amazon region corresponds to approximately 40% of the cases of leishmaniasis in Brazil. We report a prospective study with 180 patients conducted in a health care unit that diagnoses 10% of the cases of leishmaniasis in the Brazilian Amazon. The study addresses how a combination of procedures improves diagnosis in areas with high prevalence of Leishmania guyanensis. Objectives: to evaluate diagnostic methods in areas with high prevalence of Leishmania guyanensis. Methods: All subjects were amastigote-positive by direct microscopic examination of lesion scarifications. We conducted skin biopsy and histopathology, polymerase chain reaction and parasite cultivation. Results: Polymerase chain reaction detected almost ninety percent of infections when two amplification protocols were used (mini-exon and HSP-70). HSP-70 specific polymerase chain reaction matched the sensitivity of parasite cultivation plus histopathology. Conclusion: The best combination was polymerase chain reaction plus histopathology, which increased diagnostic sensitivity to 94%. Species discrimination by polymerase chain reaction disclosed prevalence of human infections with Leishmania guyanensis of 94% and with Leishmania braziliensis of 6% for this region. |
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| MeSH term(s) | Adolescent ; Adult ; Aged ; Biopsy ; Brazil ; Child ; Child, Preschool ; Female ; Humans ; Leishmania braziliensis/isolation & purification ; Leishmania guyanensis/isolation & purification ; Leishmaniasis, Mucocutaneous/diagnosis ; Male ; Middle Aged ; Polymerase Chain Reaction ; Prevalence ; Prospective Studies ; Sensitivity and Specificity ; Young Adult | ||||||||||
| Language | English | ||||||||||
| Publishing date | 2012-01-15 | ||||||||||
| Publishing country | Spain | ||||||||||
| Document type | Evaluation Studies ; Journal Article | ||||||||||
| ZDB-ID | 433655-0 | ||||||||||
| ISSN | 1806-4841 ; 0365-0596 | ||||||||||
| ISSN (online) | 1806-4841 | ||||||||||
| ISSN | 0365-0596 | ||||||||||
| DOI | 10.1590/s0365-05962011000600012 | ||||||||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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