Article: Efficient adeno-associated virus serotype 5 capture with affinity functionalized nanofiber adsorbents.
Frontiers in bioengineering and biotechnology
2023 Volume 11, Page(s) 1183974
Abstract: Adeno-associated viruses (AAVs) are one of the most promising tools for gene therapy applications. These vectors are purified using affinity and ion exchange chromatography, typically using packed beds of resin adsorbents. This leads to diffusion and ... ...
| Abstract | Adeno-associated viruses (AAVs) are one of the most promising tools for gene therapy applications. These vectors are purified using affinity and ion exchange chromatography, typically using packed beds of resin adsorbents. This leads to diffusion and pressure drop limitations that affect process productivity. Due to their high surface area and porosity, electrospun nanofiber adsorbents offer mass transfer and flow rate advantages over conventional chromatographic media. The present work investigated the use of affinity cellulose-based nanofiber adsorbents for adeno-associated virus serotype 5 (AAV5) capture, evaluating dynamic binding capacity, pressure drop, and AAV5 recovery at residence times (RT) less than 5 s. The dynamic binding capacity was found to be residence time-dependent, but nevertheless higher than 1.0 × 10 |
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| Language | English |
| Publishing date | 2023-05-16 |
| Publishing country | Switzerland |
| Document type | Journal Article |
| ZDB-ID | 2719493-0 |
| ISSN | 2296-4185 |
| ISSN | 2296-4185 |
| DOI | 10.3389/fbioe.2023.1183974 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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