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  1. Article: Could biological tissue preservation methods change chemical elements proportion measured by energy dispersive X-ray spectroscopy?

    Ladeira, Luiz Carlos Maia / dos Santos, Eliziária Cardoso / Valente, Gilmar Edilberto / da Silva, Janaina / Santos, Talita Amorim / dos Santos Costa Maldonado, Izabel Regina

    Biological trace element research. 2020 July, v. 196, no. 1

    2020  

    Abstract: Energy dispersive X-ray spectroscopy (EDS) is a powerful technical tool used in the biomedical field to investigate the proportion of chemical elements of interest in research, such as heavy metal bioaccumulation and the enzymatic cofactors and ... ...

    Abstract Energy dispersive X-ray spectroscopy (EDS) is a powerful technical tool used in the biomedical field to investigate the proportion of chemical elements of interest in research, such as heavy metal bioaccumulation and the enzymatic cofactors and nanoparticle therapy in various pathologies. However, the correct evaluation of the proportion of the elements is subject to some factors, including the method of sample preservation. In this study, we seek to investigate the effect of biological tissue preservation methods on the proportion of chemical elements obtained by the EDS methodology. For such, we used EDS to measure the proportion of chemical elements with biomedical interest in preserved livers, using three common methods for preserving biological tissues: (a) freezing, (b) paraformaldehyde fixative solution, and (c) Karnovsky solution. We found an increased level of sodium and reduced contents of potassium and copper in samples fixed in fixative solutions, when compared to frozen samples (p < 0.05). Our data indicate that preservation methods can change the proportion of chemical elements in biological samples, when measured by EDS. Frozen preservation should be preferred to retain the actual chemical content of samples and allow a correct assessment of the proportion of their elements.
    Keywords bioaccumulation ; copper ; energy-dispersive X-ray analysis ; freezing ; heavy metals ; liver ; nanoparticles ; potassium ; sodium ; therapeutics
    Language English
    Dates of publication 2020-07
    Size p. 168-172.
    Publishing place Springer US
    Document type Article
    ZDB-ID 445336-0
    ISSN 1559-0720 ; 0163-4984
    ISSN (online) 1559-0720
    ISSN 0163-4984
    DOI 10.1007/s12011-019-01909-x
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Could biological tissue preservation methods change chemical elements proportion measured by energy dispersive X-ray spectroscopy?

    Ladeira, Luiz Carlos Maia / Dos Santos, Eliziária Cardoso / Valente, Gilmar Edilberto / da Silva, Janaina / Santos, Talita Amorim / Dos Santos Costa Maldonado, Izabel Regina

    Biological trace element research

    2019  Volume 196, Issue 1, Page(s) 168–172

    Abstract: Energy dispersive X-ray spectroscopy (EDS) is a powerful technical tool used in the biomedical field to investigate the proportion of chemical elements of interest in research, such as heavy metal bioaccumulation and the enzymatic cofactors and ... ...

    Abstract Energy dispersive X-ray spectroscopy (EDS) is a powerful technical tool used in the biomedical field to investigate the proportion of chemical elements of interest in research, such as heavy metal bioaccumulation and the enzymatic cofactors and nanoparticle therapy in various pathologies. However, the correct evaluation of the proportion of the elements is subject to some factors, including the method of sample preservation. In this study, we seek to investigate the effect of biological tissue preservation methods on the proportion of chemical elements obtained by the EDS methodology. For such, we used EDS to measure the proportion of chemical elements with biomedical interest in preserved livers, using three common methods for preserving biological tissues: (a) freezing, (b) paraformaldehyde fixative solution, and (c) Karnovsky solution. We found an increased level of sodium and reduced contents of potassium and copper in samples fixed in fixative solutions, when compared to frozen samples (p < 0.05). Our data indicate that preservation methods can change the proportion of chemical elements in biological samples, when measured by EDS. Frozen preservation should be preferred to retain the actual chemical content of samples and allow a correct assessment of the proportion of their elements.
    MeSH term(s) Animals ; Liver/chemistry ; Male ; Metals, Alkali/analysis ; Metals, Alkaline Earth/analysis ; Metals, Heavy/analysis ; Mice ; Spectrometry, X-Ray Emission ; Tissue Preservation
    Chemical Substances Metals, Alkali ; Metals, Alkaline Earth ; Metals, Heavy
    Language English
    Publishing date 2019-10-25
    Publishing country United States
    Document type Journal Article
    ZDB-ID 445336-0
    ISSN 1559-0720 ; 0163-4984
    ISSN (online) 1559-0720
    ISSN 0163-4984
    DOI 10.1007/s12011-019-01909-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Green tea infusion prevents diabetic nephropathy aggravation in recent-onset type 1 diabetes regardless of glycemic control.

    Ladeira, Luiz Carlos Maia / Dos Santos, Eliziária Cardoso / Santos, Talita Amorim / da Silva, Janaina / Lima, Graziela Domingues de Almeida / Machado-Neves, Mariana / da Silva, Renê Chagas / Freitas, Mariella Bontempo / Maldonado, Izabel Regina Dos Santos Costa

    Journal of ethnopharmacology

    2021  Volume 274, Page(s) 114032

    Abstract: Ethnopharmacological relevance: Green tea, traditionally used as antidiabetic medicine, positively affects the diabetic nephropathy. It was assumed that these beneficial effects were due to the hypoglycemiant capacity of the tea, wich reduces the ... ...

    Abstract Ethnopharmacological relevance: Green tea, traditionally used as antidiabetic medicine, positively affects the diabetic nephropathy. It was assumed that these beneficial effects were due to the hypoglycemiant capacity of the tea, wich reduces the glycemic overload and, consequently, the advanced glycation end products rate and oxidative damage. However, these results are still controversial, since tea is not always able to exert a hypoglycemic action, as demonstrated by previous studies.
    Aim: Investigate if green tea infusion can generate positive outcomes for the kidney independently of glycemic control, using a model of severe type 1 diabetes.
    Material and methods: We treated streptozotocin type 1 diabetic young rats with 100 mg/kg of green tea, daily, for 42 days, and evaluated the serum and tissue markers for stress and function. We also analyzed the ion dynamics in the organ and the morphological alterations promoted by diabetes and green tea treatment. Besides, we analyzed, by an in silico approach, the interactions of the green tea main catechins with the proteins expressed in the kidney.
    Results: Our findings reveal that the components of green tea can interact with the proteins participating in cell signaling pathways that regulate energy metabolism, including glucose and glycogen synthesis, glucose reabsorption, hypoxia management, and cell death by apoptosis. Such interaction reduces glycogen accumulation in the organ, and protects the DNA. These results also reflect in a preserved glomerulus morphology, with improvement in pathological features, and suggesting a prevention of kidney function impairment.
    Conclusion: Our results show that such benefits are achieved regardless of the blood glucose status, and are not dependent on the reduction of hyperglycemia.
    MeSH term(s) Animals ; Camellia sinensis ; Catalase/metabolism ; DNA Damage ; Diabetes Mellitus, Experimental/metabolism ; Diabetes Mellitus, Experimental/pathology ; Diabetes Mellitus, Experimental/therapy ; Diabetes Mellitus, Type 1/metabolism ; Diabetes Mellitus, Type 1/pathology ; Diabetes Mellitus, Type 1/therapy ; Diabetic Nephropathies/metabolism ; Diabetic Nephropathies/pathology ; Diabetic Nephropathies/therapy ; Glycemic Control ; Glycogen/metabolism ; Kidney/drug effects ; Kidney/metabolism ; Kidney/pathology ; Male ; Nitric Oxide/metabolism ; Rats, Wistar ; Superoxide Dismutase/metabolism ; Tea ; Rats
    Chemical Substances Tea ; Nitric Oxide (31C4KY9ESH) ; Glycogen (9005-79-2) ; Catalase (EC 1.11.1.6) ; Superoxide Dismutase (EC 1.15.1.1)
    Language English
    Publishing date 2021-03-15
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2021.114032
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Green tea infusion prevents diabetic nephropathy aggravation in recent-onset type 1 diabetes regardless of glycemic control

    Maia Ladeira, Luiz Carlos / Cardoso dos Santos, Eliziária / Chagas da Silva, Renê / Santos, Talita Amorim / da Silva, Janaina / Lima, Graziela Domingues de Almeida / Machado-Neves, Mariana / da Silva, Renê Chagas / Freitas, Mariella Bontempo / Maldonado, Izabel Regina dos Santos Costa

    Journal of ethnopharmacology. 2021 June 28, v. 274

    2021  

    Abstract: Green tea, traditionally used as antidiabetic medicine, positively affects the diabetic nephropathy. It was assumed that these beneficial effects were due to the hypoglycemiant capacity of the tea, wich reduces the glycemic overload and, consequently, ... ...

    Abstract Green tea, traditionally used as antidiabetic medicine, positively affects the diabetic nephropathy. It was assumed that these beneficial effects were due to the hypoglycemiant capacity of the tea, wich reduces the glycemic overload and, consequently, the advanced glycation end products rate and oxidative damage. However, these results are still controversial, since tea is not always able to exert a hypoglycemic action, as demonstrated by previous studies.Investigate if green tea infusion can generate positive outcomes for the kidney independently of glycemic control, using a model of severe type 1 diabetes.We treated streptozotocin type 1 diabetic young rats with 100 mg/kg of green tea, daily, for 42 days, and evaluated the serum and tissue markers for stress and function. We also analyzed the ion dynamics in the organ and the morphological alterations promoted by diabetes and green tea treatment. Besides, we analyzed, by an in silico approach, the interactions of the green tea main catechins with the proteins expressed in the kidney.Our findings reveal that the components of green tea can interact with the proteins participating in cell signaling pathways that regulate energy metabolism, including glucose and glycogen synthesis, glucose reabsorption, hypoxia management, and cell death by apoptosis. Such interaction reduces glycogen accumulation in the organ, and protects the DNA. These results also reflect in a preserved glomerulus morphology, with improvement in pathological features, and suggesting a prevention of kidney function impairment.Our results show that such benefits are achieved regardless of the blood glucose status, and are not dependent on the reduction of hyperglycemia.
    Keywords DNA ; apoptosis ; blood glucose ; blood serum ; computer simulation ; diabetic nephropathy ; energy metabolism ; flavanols ; glucose ; glycemic control ; glycogen ; green tea ; hyperglycemia ; hypoxia ; insulin-dependent diabetes mellitus ; kidneys ; models ; renal function ; resorption ; streptozotocin ; traditional medicine
    Language English
    Dates of publication 2021-0628
    Publishing place Elsevier B.V.
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2021.114032
    Database NAL-Catalogue (AGRICOLA)

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