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Article ; Online: Bioproduction of succinic acid from potato waste. Kinetic modeling

Escanciano, Itziar A. / Santos, Victoria E. / Blanco, Angeles / Ladero, Miguel

Industrial Crops & Products. 2023 Nov., v. 203 p.117124-

2023

Abstract: Succinic acid is a key chemical platform for the implementation of biorefineries and, therefore, for the development of a sustainable bioeconomy. In this work, succinic acid anaerobic production by Actinobacillus succinogenes from potato waste and ... ...

Abstract	Succinic acid is a key chemical platform for the implementation of biorefineries and, therefore, for the development of a sustainable bioeconomy. In this work, succinic acid anaerobic production by Actinobacillus succinogenes from potato waste and glucose was carried out. Firstly, a simple kinetic model was developed to accurately predict the evolution of succinic acid, substrate, by-products, and biomass based on bottle experiments performed at different initial glucose concentrations. Secondly, experiments with an acid hydrolysate of potato waste as carbon source were performed in orbitally shaken bottles and bioreactor to compare the fermentation performance of the potato waste with the pure glucose. Using this hydrolysate, 32.2 g‧L⁻¹ of succinic acid were obtained with a productivity of 0.64 g‧L⁻¹‧h⁻¹, improving SA yield by 37% compared to results with pure glucose. This is one of the highest values to date for succinic acid bioproduction from food residues (0.92 g‧g⁻¹). Finally, the proposed kinetic model was successfully applied to the fermentation data obtained from potato waste fermentation, being this type of models a highly valuable tool for future techno-economic analysis of the bioprocess.
Keywords	Actinobacillus succinogenes ; bioeconomics ; biomass ; bioprocessing ; bioreactors ; biorefining ; carbon ; evolution ; fermentation ; glucose ; hydrolysates ; kinetics ; potatoes ; succinic acid ; wastes ; Potato waste ; Hydrolysis ; Kinetic model ; CO2
Language	English
Dates of publication	2023-11
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	1132158-1
ISSN	1872-633X ; 0926-6690
ISSN (online)	1872-633X
ISSN	0926-6690
DOI	10.1016/j.indcrop.2023.117124
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Study on the Operational Modes Using Both Growing and Resting Cells for Succinic Acid Production from Xylose Kinetic Modelling

Escanciano, Itziar A. / Ripoll, Vanessa / Ladero, Miguel / Santos, Victoria E.

Fermentation. 2023 July 14, v. 9, no. 7

2023

Abstract: Succinic acid (SA) is one of the most prominent C4 biomass-based platform chemicals that can be biologically obtained. This article verifies, for the first time, the possibility of producing succinic acid with fed-batch or repeated batch operations with ... ...

Abstract	Succinic acid (SA) is one of the most prominent C4 biomass-based platform chemicals that can be biologically obtained. This article verifies, for the first time, the possibility of producing succinic acid with fed-batch or repeated batch operations with Actinobacillus succinogenes in a resting state, that is, in the absence of a nitrogen source. In this work it is possible to optimise separately the stages of cell growth and production in the fed-batch or repeated batch modes, minimising the costs associated with the nitrogen source and facilitating the subsequent purification of SA. These experiments were carried out with xylose, the most abundant monosaccharide in hemicelluloses, with the results subsequently being compared to those obtained in equivalent operations carried out with cells in a state of growth. First, a cost-effective synthetic growth medium was proposed and successfully employed for SA production. Biocatalysts’ reutilisation showed that the bioprocess can be carried out successfully in repeated batch and fed-batch modes. The best mode for growing cells is repeated batch, achieving a maximum productivity of 0.77 g‧L⁻¹‧h⁻¹, a selectivity of 53% and a yield of 51% with respect to xylose consumed. In contrast, the fed-batch mode was found to be the most convenient mode with resting cell biocatalyst, reaching a maximum productivity of 0.83 g‧L⁻¹‧h⁻¹, a selectivity of 0.78 g‧g⁻¹ and a yield of 68% with respect to the xylose consumed. In addition, by-product formation is significantly reduced when employing resting cells. An unstructured non-segregated kinetic model was developed for both biocatalysts, capable of simulating cell growth, xylose consumption, SA production and by-product generation, with successful estimation of kinetic parameters supported by statistical criteria.
Keywords	Actinobacillus succinogenes ; biocatalysts ; bioprocessing ; byproducts ; cell growth ; cost effectiveness ; culture media ; fermentation ; hemicellulose ; kinetics ; nitrogen ; succinic acid ; xylose
Language	English
Dates of publication	2023-0714
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2813985-9
ISSN	2311-5637
ISSN	2311-5637
DOI	10.3390/fermentation9070663
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Development of a Simple and Robust Kinetic Model for the Production of Succinic Acid from Glucose Depending on Different Operating Conditions

Escanciano, Itziar A. / Ladero, Miguel / Santos, Victoria E. / Blanco, Angeles

Fermentation. 2023 Feb. 25, v. 9, no. 3

2023

Abstract: Succinic acid (SA) is one of the main identified biomass-derived chemical building blocks. In this work we approach the study of its production by Actinobacillus succinogenes DSM 22257 from glucose, focusing on the development and application of a simple ...

Abstract	Succinic acid (SA) is one of the main identified biomass-derived chemical building blocks. In this work we approach the study of its production by Actinobacillus succinogenes DSM 22257 from glucose, focusing on the development and application of a simple kinetic model capable of representing the evolution of the process over time for a great diversity of process variables key to the production of this platform bio-based chemical: initial biomass concentration, yeast extract concentration, agitation speed, and carbon dioxide flow rate. All these variables were studied experimentally, determining the values of key fermentation parameters: titer (23.8–39.7 g·L⁻¹), yield (0.59–0.72 gSA·ggₗᵤ⁻¹), productivity (0.48–0.96 gSA·L⁻¹·h⁻¹), and selectivity (0.61–0.69 gSA·ggₗᵤ⁻¹). Even with this wide diversity of operational conditions, a non-structured and non-segregated kinetic model was suitable for fitting to experimental data with high accuracy, considering the values of the goodness-of-fit statistical parameters. This model is based on the logistic equation for biomass growth and on potential kinetic equations to describe the evolution of SA and the sum of by-products as production events that are not associated with biomass growth. The application of the kinetic model to diverse operational conditions sheds light on their effect on SA production. It seems that nitrogen stress is a good condition for SA titer and selectivity, there is an optimal inoculum mass for this purpose, and hydrodynamic stress starts at 300 r.p.m. in the experimental set-up employed. Due to its practical importance, and to validate the developed kinetic model, a fed-batch fermentation was also carried out, verifying the goodness of the model proposed via the process simulation (stage or cycle 1) and application to further cycles of the fed-batch operation. The results showed that biomass inactivation started at cycle 3 after a grace period in cycle 2.
Keywords	Actinobacillus succinogenes ; agitation ; batch fermentation ; biomass ; carbon dioxide ; equations ; evolution ; glucose ; hydrodynamics ; inoculum ; kinetics ; models ; nitrogen ; succinic acid ; yeast extract
Language	English
Dates of publication	2023-0225
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2813985-9
ISSN	2311-5637
ISSN	2311-5637
DOI	10.3390/fermentation9030222
Database	NAL-Catalogue (AGRICOLA)

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Article: Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration

Ripoll, Vanessa / Ladero, Miguel / Santos, Victoria E.

Biochemical engineering journal. 2021 Dec., v. 176

2021

Abstract: Biodiesel-derived glycerol was biologically converted to 2,3-butanediol, an organic compound with multiple industrial applications. Recent studies showed that Raoultella terrigena CECT 4519 is an effective biocatalyst for the bioprocess under ... ...

Abstract	Biodiesel-derived glycerol was biologically converted to 2,3-butanediol, an organic compound with multiple industrial applications. Recent studies showed that Raoultella terrigena CECT 4519 is an effective biocatalyst for the bioprocess under conventional growing conditions. In the present work, a novel biocatalyst composed by R. terrigena resting cells was evaluated for the first time. Fluid dynamic conditions has been optimized to maximize 2,3-butanediol production in terms of titre, yield, and selectivity. Regarding the effect of initial glycerol concentration in batch runs, no substrate inhibition was detected in the studied conditions (concentrations between 45 and 250 g/L were used). Employing pure glycerol as carbon source, 82.0 g/L 2,3-butanediol titre was achieved, whereas 76.5 g/L were reached using raw glycerol. These numbers involve an achieved yield respect to maximal theoretic yield of 79% and 77%, respectively. A successful kinetic modelling of the bioprocess was developed and it is able to describe both the evolution of concentrations of relevant components with time and the rates calculated at experimental time values. Estimated specific 2,3-butanediol production rate was 0.034 g₂,₃₋BDO/gX·h, while the estimated empirical pseudo-stoichiometric coefficient glycerol/2,3-butanediol was 2.52 gGₗy/g₂,₃₋BDO. The obtained results showed that R. terrigena resting cells is a promising biocatalyst, which provides new opportunities for developing and scaling-up glycerol biorefineries.
Keywords	Raoultella terrigena ; biocatalysts ; bioprocessing ; biorefining ; carbon ; evolution ; fluid mechanics ; glycerol
Language	English
Dates of publication	2021-12
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2012139-8
ISSN	1369-703X
ISSN	1369-703X
DOI	10.1016/j.bej.2021.108185
Database	NAL-Catalogue (AGRICOLA)

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Article: Modeling the Succinic Acid Bioprocess: A Review

Escanciano, Itziar A. / Wojtusik, Mateusz / Esteban, Jesús / Ladero, Miguel / Santos, Victoria E.

Fermentation. 2022 July 31, v. 8, no. 8

2022

Abstract: Succinic acid has attracted much interest as a key platform chemical that can be obtained in high titers from biomass through sustainable fermentation processes, thus boosting the bioeconomy as a critical production strategy for the future. After several ...

Abstract	Succinic acid has attracted much interest as a key platform chemical that can be obtained in high titers from biomass through sustainable fermentation processes, thus boosting the bioeconomy as a critical production strategy for the future. After several years of development of the production of succinic acid, many studies on lab or pilot scale production have been reported. The relevant experimental data reveal underlying physical and chemical dynamic phenomena. To take advantage of this vast, but disperse, kinetic information, a number of mathematical kinetic models of the unstructured non-segregated type have been proposed in the first place. These relatively simple models feature critical aspects of interest for the design, control, optimization and operation of this key bioprocess. This review includes a detailed description of the phenomena involved in the bioprocesses and how they reflect on the most important and recent models based on macroscopic and metabolic chemical kinetics, and in some cases even coupling mass transport.
Keywords	bioeconomics ; biomass ; bioprocessing ; fermentation ; mass transfer ; reaction kinetics ; succinic acid
Language	English
Dates of publication	2022-0731
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2813985-9
ISSN	2311-5637
ISSN	2311-5637
DOI	10.3390/fermentation8080368
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Resting cells isobutanol production by Shimwellia blattae (p424IbPSO): Influence of growth culture conditions.

Acedos, Miguel G / Santos, Victoria E / Garcia-Ochoa, Felix

Biotechnology progress

2018 Volume 34, Issue 5, Page(s) 1073–1080

Abstract: Isobutanol is a promising gasoline additive and could even be a potential substitute used directly as combustible. In this work, the production of isobutanol from glucose by Shimwellia blattae (p424IbPSO) in resting cell cultures is studied. This ... ...

Abstract	Isobutanol is a promising gasoline additive and could even be a potential substitute used directly as combustible. In this work, the production of isobutanol from glucose by Shimwellia blattae (p424IbPSO) in resting cell cultures is studied. This production has two stages, involving a resting cell phase that has not been studied before. The cell growth was carried out under different operating conditions: temperature and medium composition (YE, ammonium, and IPTG concentrations), looking for the highest isobutanol production. Moreover, the cells were collected at three different growth times checking their isobutanol production capacity. The best operating conditions have been determined as: 30°C of temperature, a medium containing 1.5 g L
MeSH term(s)	Butanols/metabolism ; Culture Media ; Enterobacteriaceae/metabolism ; Glucose/metabolism ; Temperature
Chemical Substances	Butanols ; Culture Media ; isobutyl alcohol (56F9Z98TEM) ; Glucose (IY9XDZ35W2)
Language	English
Publishing date	2018-10-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	165657-0
ISSN	1520-6033 ; 8756-7938
ISSN (online)	1520-6033
ISSN	8756-7938
DOI	10.1002/btpr.2705
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Article: High 2,3-butanediol production from glycerol by Raoultella terrigena CECT 4519

Ripoll, Vanessa / Rodríguez, Alberto / Ladero, Miguel / Santos, Victoria E

Bioprocess and biosystems engineering. 2020 Apr., v. 43, no. 4

2020

Abstract: Bioconversion of biodiesel-derived glycerol into 2,3-butanediol has received recently much attention due to its increasing surplus and its multiple uses in industry as bulk chemical. The influence of initial glycerol concentration on 2,3-butanediol ... ...

Abstract	Bioconversion of biodiesel-derived glycerol into 2,3-butanediol has received recently much attention due to its increasing surplus and its multiple uses in industry as bulk chemical. The influence of initial glycerol concentration on 2,3-butanediol production in batch runs has been studied. A concentration higher than 140 g/L produces an inhibitory effect on the final 2,3-butanediol concentration and its production rate. In batch mode, the highest yield respect to the theoretical maximum yield (71%) was reached employing 140 g/L as initial concentration 140 g/L. Based on these results, a high 2,3-butanediol production has been achieved through a fed-batch strategy. The reached 2,3-butanediol concentration was 90.5 g/L from pure glycerol and 80.5 g/L from raw glycerol. The 2,3-butanediol yield respect to the theoretical maximum yield was also improved through the fed-batch operation (90%). To date, this concentration is the highest produced amount employing as biocatalyst a non-pathogenic bacterium (level 1).
Keywords	Raoultella terrigena ; bacteria ; biocatalysts ; biotransformation ; glycerol ; industry
Language	English
Dates of publication	2020-04
Size	p. 685-692.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	1476357-6
ISSN	1432-0797 ; 1615-7605 ; 1615-7591
ISSN (online)	1432-0797 ; 1615-7605
ISSN	1615-7591
DOI	10.1007/s00449-019-02266-8
Database	NAL-Catalogue (AGRICOLA)

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Article: Fluid dynamic conditions and oxygen availability effects on microbial cultures in STBR: An overview

Garcia-Ochoa, Felix / Gomez, Emilio / Santos, Victoria E

Biochemical engineering journal. 2020 Dec. 15, v. 164

2020

Abstract: Bioprocesses are often carried out in stirred tank bioreactors. Due to the need of mixing, homogenization and interphase transports, cultures are usually agitated by mechanical, hydraulic or pneumatic means. The increasing in agitation decreases mixing ... ...

Abstract	Bioprocesses are often carried out in stirred tank bioreactors. Due to the need of mixing, homogenization and interphase transports, cultures are usually agitated by mechanical, hydraulic or pneumatic means. The increasing in agitation decreases mixing time, improves homogeneity, mass and heat transfer rates and, accordingly, the metabolism and the biochemical reaction rates are also influenced. The power input into the system is essentially required in order to increase the mass transfer rate, the oxygen transport rate in aerobic bioprocesses, but also the shear forces are increased and consequently the concentration of some compounds in the liquid phase (oxygen in the aerobic bioprocess) with the corresponding effects on the cellular metabolism and the cellular structures due to fluid dynamics into the bioreactor. Hydrodynamic conditions in STBR are given by the broth characteristic (mainly viscosity), the vessel geometry, including the stirrer and sparger design, and the agitation and gas flow rate. Thus, shear effects would take place, affecting the culture performance, provoking cell damage in some occasions. The primary goal of this work is to describe up to date knowledge on these concepts: fluid dynamic conditions, hydrodynamic stress and oxygen availability influence affecting the microbial processes performance.
Keywords	agitation ; bioprocessing ; bioreactors ; geometry ; heat transfer ; homogenization ; hydrodynamics ; interphase ; liquids ; mass transfer ; metabolism ; oxygen ; viscosity
Language	English
Dates of publication	2020-1215
Publishing place	Elsevier B.V.
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	2012139-8
ISSN	1369-703X
ISSN	1369-703X
DOI	10.1016/j.bej.2020.107803
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: High 2,3-butanediol production from glycerol by Raoultella terrigena CECT 4519.

Ripoll, Vanessa / Rodríguez, Alberto / Ladero, Miguel / Santos, Victoria E

Bioprocess and biosystems engineering

2019 Volume 43, Issue 4, Page(s) 685–692

Abstract	Bioconversion of biodiesel-derived glycerol into 2,3-butanediol has received recently much attention due to its increasing surplus and its multiple uses in industry as bulk chemical. The influence of initial glycerol concentration on 2,3-butanediol production in batch runs has been studied. A concentration higher than 140 g/L produces an inhibitory effect on the final 2,3-butanediol concentration and its production rate. In batch mode, the highest yield respect to the theoretical maximum yield (71%) was reached employing 140 g/L as initial concentration 140 g/L. Based on these results, a high 2,3-butanediol production has been achieved through a fed-batch strategy. The reached 2,3-butanediol concentration was 90.5 g/L from pure glycerol and 80.5 g/L from raw glycerol. The 2,3-butanediol yield respect to the theoretical maximum yield was also improved through the fed-batch operation (90%). To date, this concentration is the highest produced amount employing as biocatalyst a non-pathogenic bacterium (level 1).
MeSH term(s)	Batch Cell Culture Techniques ; Bioreactors ; Butylene Glycols/metabolism ; Enterobacteriaceae/metabolism ; Glycerol/metabolism
Chemical Substances	Butylene Glycols ; 2,3-butylene glycol (45427ZB5IJ) ; Glycerol (PDC6A3C0OX)
Language	English
Publishing date	2019-12-17
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1476357-6
ISSN	1615-7605 ; 1432-0797 ; 1615-7591
ISSN (online)	1615-7605 ; 1432-0797
ISSN	1615-7591
DOI	10.1007/s00449-019-02266-8
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Article: Modulating redox metabolism to improve isobutanol production in Shimwellia blattae

Acedos, Miguel G / de la Torre, Isabel / Santos, Victoria E / García-Ochoa, Félix / García, José L / Galán, Beatriz

Biotechnology for biofuels. 2021 Dec., v. 14, no. 1

2021

Abstract: BACKGROUND: Isobutanol is a candidate to replace gasoline from fossil resources. This higher alcohol can be produced from sugars using genetically modified microorganisms. Shimwellia blattae (p424IbPSO) is a robust strain resistant to high concentration ... ...

Abstract	BACKGROUND: Isobutanol is a candidate to replace gasoline from fossil resources. This higher alcohol can be produced from sugars using genetically modified microorganisms. Shimwellia blattae (p424IbPSO) is a robust strain resistant to high concentration of isobutanol that can achieve a high production rate of this alcohol. Nevertheless, this strain, like most strains developed for isobutanol production, has some limitations in its metabolic pathway. Isobutanol production under anaerobic conditions leads to a depletion of NADPH, which is necessary for two enzymes in the metabolic pathway. In this work, two independent approaches have been studied to mitigate the co-substrates imbalance: (i) using a NADH-dependent alcohol dehydrogenase to reduce the NADPH dependence of the pathway and (ii) using a transhydrogenase to increase NADPH level. RESULTS: The addition of the NADH-dependent alcohol dehydrogenase from Lactococcus lactis (AdhA) to S. blattae (p424IbPSO) resulted in a 19.3% higher isobutanol production. The recombinant strain S. blattae (p424IbPSO, pIZpntAB) harboring the PntAB transhydrogenase produced 39.0% more isobutanol than the original strain, reaching 5.98 g L⁻¹ of isobutanol. In both strains, we observed a significant decrease in the yields of by-products such as lactic acid or ethanol. CONCLUSIONS: The isobutanol biosynthesis pathway in S. blattae (p424IbPSO) uses the endogenous NADPH-dependent alcohol dehydrogenase YqhD to complete the pathway. The addition of NADH-dependent AdhA leads to a reduction in the consumption of NADPH that is a bottleneck of the pathway. The higher consumption of NADH by AdhA reduces the availability of NADH required for the transformation of pyruvate into lactic acid and ethanol. On the other hand, the expression of PntAB from E. coli increases the availability of NADPH for IlvC and YqhD and at the same time reduces the availability of NADH and thus, the production of lactic acid and ethanol. In this work it is shown how the expression of AdhA and PntAB enzymes in Shimwellia blattae increases yield from 11.9% to 14.4% and 16.4%, respectively.
Keywords	Escherichia coli ; Lactococcus lactis ; alcohol dehydrogenase ; anaerobic conditions ; biochemical pathways ; biofuels ; biosynthesis ; biotechnology ; byproducts ; ethanol ; gasoline ; lactic acid ; microorganisms ; mineral resources ; pyruvic acid ; strains ; sugars ; yields
Language	English
Dates of publication	2021-12
Size	p. 8.
Publishing place	BioMed Central
Document type	Article
ZDB-ID	2421351-2
ISSN	1754-6834
ISSN	1754-6834
DOI	10.1186/s13068-020-01862-1
Database	NAL-Catalogue (AGRICOLA)

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