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  1. Article ; Online: Immunohistochemical Techniques for Phosphoproteins.

    Sanz Ressel, Berenice L / Molinolo, Alfredo A

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2593, Page(s) 259–264

    Abstract: The use of immunohistochemical techniques to study the patterns of protein phosphorylation has revolutionized the study of signaling pathways. This technique allows detecting the phosphorylated state of signaling proteins in formalin-fixed and paraffin- ... ...

    Abstract The use of immunohistochemical techniques to study the patterns of protein phosphorylation has revolutionized the study of signaling pathways. This technique allows detecting the phosphorylated state of signaling proteins in formalin-fixed and paraffin-embedded tissue sections by using phosphospecific antibodies. This chapter describes in detail the immunohistocshemical protocols from which the study of phosphoproteins in tissue sections can be approached.
    MeSH term(s) Paraffin Embedding ; Phosphoproteins ; Immunohistochemistry ; Formaldehyde
    Chemical Substances Phosphoproteins ; Formaldehyde (1HG84L3525)
    Language English
    Publishing date 2022-12-13
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2811-9_17
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  2. Article ; Online: Altered E-cadherin/β-catenin expression in feline cutaneous squamous cell carcinomas.

    Sanz Ressel, Berenice L / Gomez Castro, Gimena / Massone, Adriana R / Barbeito, Claudio G

    Journal of comparative pathology

    2023  Volume 207, Page(s) 21–24

    Abstract: Cutaneous squamous cell carcinoma (CSCC) is the most common malignant skin tumour in cats and the nature of the molecular mechanisms involved is poorly defined. Included among the molecular mechanisms in human and canine CSCCs is altered expression of E- ... ...

    Abstract Cutaneous squamous cell carcinoma (CSCC) is the most common malignant skin tumour in cats and the nature of the molecular mechanisms involved is poorly defined. Included among the molecular mechanisms in human and canine CSCCs is altered expression of E-cadherin/β-catenin. This study aimed to explore the immunohistochemical expression pattern of E-cadherin and β-catenin in 43 samples of feline CSCC by using a tissue microarray to elucidate whether expression of these molecules is dysregulated. Membrane expression of E-cadherin and membrane and cytoplasmic expression of β-catenin were significantly reduced in the CSCCs. Cytoplasmic expression of E-cadherin and nuclear expression of β-catenin were also found in some CSCCs. These findings indicate that altered expression of E-cadherin and β-catenin is a frequent event in feline CSCCs, suggesting that these molecules play an important role in acquisition of the malignant phenotype in feline patients with CSCC. The results also suggest the existence of a subpopulation of feline patients with CSCC in which the Wnt pathway may contribute to epidermal carcinogenesis.
    MeSH term(s) Cats ; Animals ; Humans ; Dogs ; beta Catenin/metabolism ; Carcinoma, Squamous Cell/pathology ; Carcinoma, Squamous Cell/veterinary ; Skin Neoplasms/pathology ; Skin Neoplasms/veterinary ; Cadherins ; Carcinogenesis ; Cat Diseases ; Dog Diseases/metabolism
    Chemical Substances beta Catenin ; Cadherins
    Language English
    Publishing date 2023-11-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 390920-7
    ISSN 1532-3129 ; 0021-9975
    ISSN (online) 1532-3129
    ISSN 0021-9975
    DOI 10.1016/j.jcpa.2023.09.007
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  3. Article ; Online: Persistent activation of the mammalian target of rapamycin signalling pathway in cutaneous squamous cell carcinomas in cats.

    Sanz Ressel, Berenice L / Massone, Adriana R / Barbeito, Claudio G

    Veterinary dermatology

    2021  Volume 32, Issue 6, Page(s) 675–e180

    Abstract: Background: Cutaneous squamous cell carcinoma (CSCC) represents the most common malignant tumour of the feline skin. Emerging evidence suggests that the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) signalling pathway ... ...

    Abstract Background: Cutaneous squamous cell carcinoma (CSCC) represents the most common malignant tumour of the feline skin. Emerging evidence suggests that the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) signalling pathway may represent a potential target for pharmacological intervention in human and canine CSCC.
    Hypothesis/objectives: The present study aimed to explore the expression pattern and status of activation of relevant signalling proteins of the PI3K/Akt/mTOR signalling pathway in feline CSCC.
    Methods and materials: The expression of pEGFR
    Results: The immunodetection using phosphospecific antibodies to detect the activated forms of signalling proteins showed that the PI3K/Akt/mTOR signalling pathway is frequently activated in feline CSCCs, and may be independent of the activation of EGFR. The results also showed that PTEN expression is not significantly altered in feline CSCCs.
    Conclusions and clinical importance: Our study shows that the persistent activation of the PI3K/Akt/mTOR signalling pathway represents a key event in feline CSCC, pointing to this signalling pathway being a potential therapeutic target in feline patients with CSCC.
    MeSH term(s) Animals ; Carcinoma, Squamous Cell/veterinary ; Cat Diseases ; Cats ; Phosphatidylinositol 3-Kinases ; Sirolimus ; Skin Neoplasms/veterinary ; TOR Serine-Threonine Kinases/genetics
    Chemical Substances TOR Serine-Threonine Kinases (EC 2.7.11.1) ; Sirolimus (W36ZG6FT64)
    Language English
    Publishing date 2021-07-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 2011122-8
    ISSN 1365-3164 ; 0959-4493
    ISSN (online) 1365-3164
    ISSN 0959-4493
    DOI 10.1111/vde.13001
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  4. Article: Persistent activation of the mammalian target of rapamycin signalling pathway in cutaneous squamous cell carcinomas in cats

    Sanz Ressel, Berenice L. / Massone, Adriana R. / Barbeito, Claudio G.

    Veterinary dermatology. 2021 Dec., v. 32, no. 6

    2021  

    Abstract: BACKGROUND: Cutaneous squamous cell carcinoma (CSCC) represents the most common malignant tumour of the feline skin. Emerging evidence suggests that the phosphatidylinositol 3‐kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) signalling pathway ... ...

    Abstract BACKGROUND: Cutaneous squamous cell carcinoma (CSCC) represents the most common malignant tumour of the feline skin. Emerging evidence suggests that the phosphatidylinositol 3‐kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) signalling pathway may represent a potential target for pharmacological intervention in human and canine CSCC. HYPOTHESIS/OBJECTIVES: The present study aimed to explore the expression pattern and status of activation of relevant signalling proteins of the PI3K/Akt/mTOR signalling pathway in feline CSCC. METHODS AND MATERIALS: The expression of pEGFRᵀʸʳ¹⁰⁶⁸, pAktSᵉʳ⁴⁷³, pS6Sᵉʳ²³⁵/²³⁶ combined with Ki‐67, and the tumour suppressor protein PTEN was evaluated by immunohistochemical analysis in 45 samples of feline CSCC, using a tissue microarray. RESULTS: The immunodetection using phosphospecific antibodies to detect the activated forms of signalling proteins showed that the PI3K/Akt/mTOR signalling pathway is frequently activated in feline CSCCs, and may be independent of the activation of EGFR. The results also showed that PTEN expression is not significantly altered in feline CSCCs. CONCLUSIONS AND CLINICAL IMPORTANCE: Our study shows that the persistent activation of the PI3K/Akt/mTOR signalling pathway represents a key event in feline CSCC, pointing to this signalling pathway being a potential therapeutic target in feline patients with CSCC.
    Keywords cats ; dogs ; humans ; immunohistochemistry ; microarray technology ; rapamycin ; squamous cell carcinoma ; therapeutics ; veterinary medicine
    Language English
    Dates of publication 2021-12
    Size p. 675-e180.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 2011122-8
    ISSN 1365-3164 ; 0959-4493
    ISSN (online) 1365-3164
    ISSN 0959-4493
    DOI 10.1111/vde.13001
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  5. Article ; Online: Dysregulated expression of the key effectors of the mammalian target of rapamycin signalling pathway in cutaneous papillomas of dogs.

    Sanz Ressel, Berenice L / Massone, Adriana R / Barbeito, Claudio G

    Veterinary and comparative oncology

    2019  Volume 17, Issue 4, Page(s) 522–527

    Abstract: Cutaneous papillomas (CP) are one of the most common skin neoplasms in dogs. Different murine models have shown that persistent activation of the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway has a central role ... ...

    Abstract Cutaneous papillomas (CP) are one of the most common skin neoplasms in dogs. Different murine models have shown that persistent activation of the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway has a central role in the development and progression of CP. The purpose of this study were to evaluate the immunohistochemical expression pattern of two key molecules involved in the PI3K/Akt/mTOR signalling pathway, pAkt
    MeSH term(s) Animals ; Dog Diseases/metabolism ; Dogs ; Gene Expression Regulation ; Papilloma/veterinary ; Signal Transduction/physiology ; Skin Neoplasms/metabolism ; Skin Neoplasms/veterinary ; TOR Serine-Threonine Kinases/genetics ; TOR Serine-Threonine Kinases/metabolism ; Tissue Array Analysis
    Chemical Substances TOR Serine-Threonine Kinases (EC 2.7.1.1)
    Language English
    Publishing date 2019-07-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 2129634-0
    ISSN 1476-5829 ; 1476-5810
    ISSN (online) 1476-5829
    ISSN 1476-5810
    DOI 10.1111/vco.12516
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  6. Article: Dysregulated expression of the key effectors of the mammalian target of rapamycin signalling pathway in cutaneous papillomas of dogs

    Sanz Ressel, Berenice L / Massone, Adriana R / Barbeito, Claudio G

    Veterinary and comparative oncology. 2019 Dec., v. 17, no. 4

    2019  

    Abstract: Cutaneous papillomas (CP) are one of the most common skin neoplasms in dogs. Different murine models have shown that persistent activation of the phosphatidylinositol 3‐kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway has a central role ... ...

    Abstract Cutaneous papillomas (CP) are one of the most common skin neoplasms in dogs. Different murine models have shown that persistent activation of the phosphatidylinositol 3‐kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway has a central role in the development and progression of CP. The purpose of this study were to evaluate the immunohistochemical expression pattern of two key molecules involved in the PI3K/Akt/mTOR signalling pathway, pAktSer473, and pS6Ser235/236, on 36 canine specimens of CP using a tissue microarray. The results show that the PI3K/Akt/mTOR signalling pathway is persistently activated in CP of dogs, pointing to this pathway as a potential therapeutic target.
    Keywords animal models ; dogs ; immunohistochemistry ; microarray technology ; phosphatidylinositol 3-kinase ; rapamycin ; signal transduction ; skin neoplasms ; therapeutics
    Language English
    Dates of publication 2019-12
    Size p. 522-527.
    Publishing place Blackwell Publishing Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 2129634-0
    ISSN 1476-5829 ; 1476-5810
    ISSN (online) 1476-5829
    ISSN 1476-5810
    DOI 10.1111/vco.12516
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