LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 4 of total 4

Search options

  1. Article ; Online: GHEP-ISFG collaborative exercise on mixture profiles (GHEP-MIX06). Reporting conclusions: Results and evaluation.

    Barrio, P A / Crespillo, M / Luque, J A / Aler, M / Baeza-Richer, C / Baldassarri, L / Carnevali, E / Coufalova, P / Flores, I / García, O / García, M A / González, R / Hernández, A / Inglés, V / Luque, G M / Mosquera-Miguel, A / Pedrosa, S / Pontes, M L / Porto, M J /
    Posada, Y / Ramella, M I / Ribeiro, T / Riego, E / Sala, A / Saragoni, V G / Serrano, A / Vannelli, S

    Forensic science international. Genetics

    2018  Volume 35, Page(s) 156–163

    Abstract: One of the main goals of the Spanish and Portuguese-Speaking Group of the International Society for Forensic Genetics (GHEP-ISFG) is to promote and contribute to the development and dissemination of scientific knowledge in the field of forensic genetics. ...

    Abstract One of the main goals of the Spanish and Portuguese-Speaking Group of the International Society for Forensic Genetics (GHEP-ISFG) is to promote and contribute to the development and dissemination of scientific knowledge in the field of forensic genetics. Due to this fact, GHEP-ISFG holds different working commissions that are set up to develop activities in scientific aspects of general interest. One of them, the Mixture Commission of GHEP-ISFG, has organized annually, since 2009, a collaborative exercise on analysis and interpretation of autosomal short tandem repeat (STR) mixture profiles. Until now, six exercises have been organized. At the present edition (GHEP-MIX06), with 25 participant laboratories, the exercise main aim was to assess mixture profiles results by issuing a report, from the proposal of a complex mock case. One of the conclusions obtained from this exercise is the increasing tendency of participating laboratories to validate DNA mixture profiles analysis following international recommendations. However, the results have shown some differences among them regarding the edition and also the interpretation of mixture profiles. Besides, although the last revision of ISO/IEC 17025:2017 gives indications of how results should be reported, not all laboratories strictly follow their recommendations. Regarding the statistical aspect, all those laboratories that have performed statistical evaluation of the data have employed the likelihood ratio (LR) as a parameter to evaluate the statistical compatibility. However, LR values obtained show a wide range of variation. This fact could not be attributed to the software employed, since the vast majority of laboratories that performed LR calculation employed the same software (LRmixStudio). Thus, the final allelic composition of the edited mixture profile and the parameters employed in the software could explain this data dispersion. This highlights the need, for each laboratory, to define through internal validations its criteria for editing and interpreting mixtures, and to continuous train in software handling.
    MeSH term(s) DNA Fingerprinting/standards ; Forensic Genetics/standards ; Humans ; Laboratories/statistics & numerical data ; Likelihood Functions ; Microsatellite Repeats ; Research Report/standards ; Societies, Scientific ; Software
    Language English
    Publishing date 2018-05-18
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2493339-9
    ISSN 1878-0326 ; 1872-4973
    ISSN (online) 1878-0326
    ISSN 1872-4973
    DOI 10.1016/j.fsigen.2018.05.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6764: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: GHEP-ISFG collaborative exercise on mixture profiles of autosomal STRs (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03): results and evaluation.

    Crespillo, M / Barrio, P A / Luque, J A / Alves, C / Aler, M / Alessandrini, F / Andrade, L / Barretto, R M / Bofarull, A / Costa, S / García, M A / García, O / Gaviria, A / Gladys, A / Gorostiza, A / Hernández, A / Piñero, M Herrera / Hombreiro, L / Ibarra, A A /
    Jiménez, M J / Luque, G M / Madero, P / Martínez-Jarreta, B / Masciovecchio, M V / Modesti, N M / Moreno, F / Pagano, S / Pedrosa, S / Plaza, G / Prat, E / Puente, J / Rendo, F / Ribeiro, T / Sala, A / Santamaría, E / Saragoni, V G / Whittle, M R

    Forensic science international. Genetics

    2014  Volume 10, Page(s) 64–72

    Abstract: One of the main objectives of the Spanish and Portuguese-Speaking Group of the International Society for Forensic Genetics (GHEP-ISFG) is to promote and contribute to the development and dissemination of scientific knowledge in the area of forensic ... ...

    Abstract One of the main objectives of the Spanish and Portuguese-Speaking Group of the International Society for Forensic Genetics (GHEP-ISFG) is to promote and contribute to the development and dissemination of scientific knowledge in the area of forensic genetics. Due to this fact, GHEP-ISFG holds different working commissions that are set up to develop activities in scientific aspects of general interest. One of them, the Mixture Commission of GHEP-ISFG, has organized annually, since 2009, a collaborative exercise on analysis and interpretation of autosomal short tandem repeat (STR) mixture profiles. Until now, three exercises have been organized (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03), with 32, 24 and 17 participant laboratories respectively. The exercise aims to give a general vision by addressing, through the proposal of mock cases, aspects related to the edition of mixture profiles and the statistical treatment. The main conclusions obtained from these exercises may be summarized as follows. Firstly, the data show an increased tendency of the laboratories toward validation of DNA mixture profiles analysis following international recommendations (ISO/IEC 17025:2005). Secondly, the majority of discrepancies are mainly encountered in stutters positions (53.4%, 96.0% and 74.9%, respectively for the three editions). On the other hand, the results submitted reveal the importance of performing duplicate analysis by using different kits in order to reduce errors as much as possible. Regarding the statistical aspect (GHEP-MIX02 and 03), all participants employed the likelihood ratio (LR) parameter to evaluate the statistical compatibility and the formulas employed were quite similar. When the hypotheses to evaluate the LR value were locked by the coordinators (GHEP-MIX02) the results revealed a minor number of discrepancies that were mainly due to clerical reasons. However, the GHEP-MIX03 exercise allowed the participants to freely come up with their own hypotheses to calculate the LR value. In this situation the laboratories reported several options to explain the mock cases proposed and therefore significant differences between the final LR values were obtained. Complete information concerning the background of the criminal case is a critical aspect in order to select the adequate hypotheses to calculate the LR value. Although this should be a task for the judicial court to decide, it is important for the expert to account for the different possibilities and scenarios, and also offer this expertise to the judge. In addition, continuing education in the analysis and interpretation of mixture DNA profiles may also be a priority for the vast majority of forensic laboratories.
    MeSH term(s) Humans ; Microsatellite Repeats ; Surveys and Questionnaires
    Language English
    Publishing date 2014-02-06
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2493339-9
    ISSN 1878-0326 ; 1872-4973
    ISSN (online) 1878-0326
    ISSN 1872-4973
    DOI 10.1016/j.fsigen.2014.01.009
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6764: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: GHEP-ISFG proficiency test 2011: Paper challenge on evaluation of mitochondrial DNA results.

    Prieto, L / Alves, C / Zimmermann, B / Tagliabracci, A / Prieto, V / Montesino, M / Whittle, M R / Anjos, M J / Cardoso, S / Heinrichs, B / Hernandez, A / López-Parra, A M / Sala, A / Saragoni, V G / Burgos, G / Marino, M / Paredes, M / Mora-Torres, C A / Angulo, R /
    Chemale, G / Vullo, C / Sánchez-Simón, M / Comas, D / Puente, J / López-Cubría, C M / Modesti, N / Aler, M / Merigioli, S / Betancor, E / Pedrosa, S / Plaza, G / Masciovecchio, M V / Schneider, P M / Parson, W

    Forensic science international. Genetics

    2013  Volume 7, Issue 1, Page(s) 10–15

    Abstract: The GHEP-ISFG Working Group performed a collaborative exercise to monitor the current practice of mitochondrial (mt)DNA reporting. The participating laboratories were invited to evaluate a hypothetical case example and assess the statistical significance ...

    Abstract The GHEP-ISFG Working Group performed a collaborative exercise to monitor the current practice of mitochondrial (mt)DNA reporting. The participating laboratories were invited to evaluate a hypothetical case example and assess the statistical significance of a match between the haplotypes of a case (hair) sample and a suspect. A total of 31 forensic laboratories participated of which all but one used the EMPOP database. Nevertheless, we observed a tenfold range of reported LR values (32-333.4), which was mainly due to the selection of different reference datasets in EMPOP but also due to different applied formulae. The results suggest the need for more standardization as well as additional research to harmonize the reporting of mtDNA evidence.
    MeSH term(s) DNA, Mitochondrial/genetics ; Databases, Genetic ; Haplotypes ; Humans
    Chemical Substances DNA, Mitochondrial
    Language English
    Publishing date 2013-01
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2493339-9
    ISSN 1878-0326 ; 1872-4973
    ISSN (online) 1878-0326
    ISSN 1872-4973
    DOI 10.1016/j.fsigen.2012.04.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6764: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Analysis of uni and bi-parental markers in mixture samples: Lessons from the 22nd GHEP-ISFG Intercomparison Exercise.

    Toscanini, U / Gusmão, L / Álava Narváez, M C / Álvarez, J C / Baldassarri, L / Barbaro, A / Berardi, G / Betancor Hernández, E / Camargo, M / Carreras-Carbonell, J / Castro, J / Costa, S C / Coufalova, P / Domínguez, V / Fagundes de Carvalho, E / Ferreira, S T G / Furfuro, S / García, O / Goios, A /
    González, R / de la Vega, A González / Gorostiza, A / Hernández, A / Jiménez Moreno, S / Lareu, M V / León Almagro, A / Marino, M / Martínez, G / Miozzo, M C / Modesti, N M / Onofri, V / Pagano, S / Pardo Arias, B / Pedrosa, S / Penacino, G A / Pontes, M L / Porto, M J / Puente-Prieto, J / Pérez, R Ramírez / Ribeiro, T / Rodríguez Cardozo, B / Rodríguez Lesmes, Y M / Sala, A / Santiago, B / Saragoni, V G / Serrano, A / Streitenberger, E R / Torres Morales, M A / Vannelli Rey, S A / Velázquez Miranda, M / Whittle, M R / Fernández, K / Salas, A

    Forensic science international. Genetics

    2016  Volume 25, Page(s) 63–72

    Abstract: Since 1992, the Spanish and Portuguese-Speaking Working Group of the ISFG (GHEP-ISFG) has been organizing annual Intercomparison Exercises (IEs) coordinated by the Quality Service at the National Institute of Toxicology and Forensic Sciences (INTCF) from ...

    Abstract Since 1992, the Spanish and Portuguese-Speaking Working Group of the ISFG (GHEP-ISFG) has been organizing annual Intercomparison Exercises (IEs) coordinated by the Quality Service at the National Institute of Toxicology and Forensic Sciences (INTCF) from Madrid, aiming to provide proficiency tests for forensic DNA laboratories. Each annual exercise comprises a Basic (recently accredited under ISO/IEC 17043: 2010) and an Advanced Level, both including a kinship and a forensic module. Here, we show the results for both autosomal and sex-chromosomal STRs, and for mitochondrial DNA (mtDNA) in two samples included in the forensic modules, namely a mixture 2:1 (v/v) saliva/blood (M4) and a mixture 4:1 (v/v) saliva/semen (M8) out of the five items provided in the 2014 GHEP-ISFG IE. Discrepancies, other than typos or nomenclature errors (over the total allele calls), represented 6.5% (M4) and 4.7% (M8) for autosomal STRs, 15.4% (M4) and 7.8% (M8) for X-STRs, and 1.2% (M4) and 0.0% (M8) for Y-STRs. Drop-out and drop-in alleles were the main cause of errors, with laboratories using different criteria regarding inclusion of minor peaks and stutter bands. Commonly used commercial kits yielded different results for a micro-variant detected at locus D12S391. In addition, the analysis of electropherograms revealed that the proportions of the contributors detected in the mixtures varied among the participants. In regards to mtDNA analysis, besides important discrepancies in reporting heteroplasmies, there was no agreement for the results of sample M4. Thus, while some laboratories documented a single control region haplotype, a few reported unexpected profiles (suggesting contamination problems). For M8, most laboratories detected only the haplotype corresponding to the saliva. Although the GHEP-ISFG has already a large experience in IEs, the present multi-centric study revealed challenges that still exist related to DNA mixtures interpretation. Overall, the results emphasize the need for further research and training actions in order to improve the analysis of mixtures among the forensic practitioners.
    MeSH term(s) Amelogenin/genetics ; Blood Chemical Analysis ; Chromosomes, Human, X ; Chromosomes, Human, Y ; DNA Fingerprinting ; DNA, Mitochondrial/genetics ; Female ; Forensic Genetics ; Genetic Markers ; Haplotypes ; Humans ; Laboratories/standards ; Male ; Microsatellite Repeats ; Saliva/chemistry ; Semen/chemistry
    Chemical Substances Amelogenin ; DNA, Mitochondrial ; Genetic Markers
    Language English
    Publishing date 2016-11
    Publishing country Netherlands
    Document type Journal Article ; Multicenter Study
    ZDB-ID 2493339-9
    ISSN 1878-0326 ; 1872-4973
    ISSN (online) 1878-0326
    ISSN 1872-4973
    DOI 10.1016/j.fsigen.2016.07.010
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6764: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top