Article: A conserved Toll-like receptor-to-NF-κB signaling pathway in the endangered coral Orbicella faveolata
Developmental and comparative immunology. 2018 Feb., v. 79
2018
Abstract: Herein, we characterize the Toll-like receptor (TLR)-to-NF-κB innate immune pathway of Orbicella faveolata (Of), which is an ecologically important, disease-susceptible, reef-building coral. As compared to human TLRs, the intracellular TIR domain of Of- ... ...
| Abstract | Herein, we characterize the Toll-like receptor (TLR)-to-NF-κB innate immune pathway of Orbicella faveolata (Of), which is an ecologically important, disease-susceptible, reef-building coral. As compared to human TLRs, the intracellular TIR domain of Of-TLR is most similar to TLR4, and it can interact in vitro with the human TLR4 adapter MYD88. Treatment of O. faveolata tissue with lipopolysaccharide, a ligand for mammalian TLR4, resulted in gene expression changes consistent with NF-κB pathway mobilization. Biochemical and cell-based assays revealed that Of-NF-κB resembles the mammalian non-canonical NF-κB protein p100 in that C-terminal truncation results in translocation of Of-NF-κB to the nucleus and increases its DNA-binding and transcriptional activation activities. Moreover, human IκB kinase (IKK) and Of-IKK can both phosphorylate conserved residues in Of-NF-κB in vitro and induce C-terminal processing of Of-NF-κB in vivo. These results are the first characterization of TLR-to-NF-κB signaling proteins in an endangered coral, and suggest that these corals have conserved innate immune pathways. |
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| Keywords | coral reefs ; corals ; humans ; IKappaB kinase ; ligands ; lipopolysaccharides ; signal transduction ; Toll-like receptor 4 ; transcription factor NF-kappa B ; transcriptional activation | |||||
| Language | English | |||||
| Dates of publication | 2018-02 | |||||
| Size | p. 128-136. | |||||
| Publishing place | Elsevier Ltd | |||||
| Document type | Article | |||||
| ZDB-ID | 752411-0 | |||||
| ISSN | 1879-0089 ; 0145-305X | |||||
| ISSN (online) | 1879-0089 | |||||
| ISSN | 0145-305X | |||||
| DOI | 10.1016/j.dci.2017.10.016 | |||||
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| Database | NAL-Catalogue (AGRICOLA) |
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