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  1. Article ; Online: WRNIP1 Protects Reversed DNA Replication Forks from SLX4-Dependent Nucleolytic Cleavage

    Bartlomiej Porebski / Sebastian Wild / Sandra Kummer / Sarah Scaglione / Pierre-Henri L. Gaillard / Kerstin Gari

    iScience, Vol 21, Iss , Pp 31-

    2019  Volume 41

    Abstract: Summary: During DNA replication stress, stalled replication forks need to be stabilized to prevent fork collapse and genome instability. The AAA + ATPase WRNIP1 (Werner Helicase Interacting Protein 1) has been implicated in the protection of stalled ... ...

    Abstract Summary: During DNA replication stress, stalled replication forks need to be stabilized to prevent fork collapse and genome instability. The AAA + ATPase WRNIP1 (Werner Helicase Interacting Protein 1) has been implicated in the protection of stalled replication forks from nucleolytic degradation, but the underlying molecular mechanism has remained unclear. Here we show that WRNIP1 exerts its protective function downstream of fork reversal. Unexpectedly though, WRNIP1 is not part of the well-studied BRCA2-dependent branch of fork protection but seems to protect the junction point of reversed replication forks from SLX4-mediated endonucleolytic degradation, possibly by directly binding to reversed replication forks. This function is specific to the shorter, less abundant, and less conserved variant of WRNIP1. Overall, our data suggest that in the absence of BRCA2 and WRNIP1 different DNA substrates are generated at reversed forks but that nascent strand degradation in both cases depends on the activity of exonucleases and structure-specific endonucleases. : Biological Sciences; Molecular Biology; Cell Biology Subject Areas: Biological Sciences, Molecular Biology, Cell Biology
    Keywords Science ; Q
    Subject code 612
    Language English
    Publishing date 2019-11-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Annex 1 to

    Sergio Calabrese / Sarah Scaglione / Silvia Milazzo / Walter D'Alessandro / Nicole Bobrowski / Giovanni Bruno Giuffrida / Dario Tedesco / Francesco Parello / Mathiew Yalire

    Annals of Geophysics, Vol 57, Iss

    Passive Degassing at Nyiragongo (D.R. Congo) and Etna (Italy) Volcanoes.

    2015  

    Abstract: Volcanic Emissions The technique for the assessment of the metal output from volcanoes was based on direct (in- plume) collection of the plume on filter substrates. Gas and aerosols in the volcanic plume have been sampled from the rims of the active ... ...

    Abstract Volcanic Emissions The technique for the assessment of the metal output from volcanoes was based on direct (in- plume) collection of the plume on filter substrates. Gas and aerosols in the volcanic plume have been sampled from the rims of the active craters. [.]
    Keywords Geophysics. Cosmic physics ; QC801-809 ; Physics ; QC1-999 ; Science ; Q
    Language English
    Publishing date 2015-03-01T00:00:00Z
    Publisher Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Annex 2 to

    Sergio Calabrese / Sarah Scaglione / Silvia Milazzo / Walter D'Alessandro / Nicole Bobrowski / Giovanni Bruno Giuffrida / Dario Tedesco / Francesco Parello / Mathiew Yalire

    Annals of Geophysics, Vol 57, Iss

    Passive Degassing at Nyiragongo (D.R. Congo) and Etna (Italy) Volcanoes.

    2015  

    Abstract: Plumes [.] ...

    Abstract Plumes [.]
    Keywords Geophysics. Cosmic physics ; QC801-809 ; Physics ; QC1-999 ; Science ; Q
    Language English
    Publishing date 2015-03-01T00:00:00Z
    Publisher Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Passive degassing at Nyiragongo (D.R. Congo) and Etna (Italy) volcanoes

    Sergio Calabrese / Sarah Scaglione / Silvia Milazzo / Walter D'Alessandro / Nicole Bobrowski / Giovanni Bruno Giuffrida / Dario Tedesco / Francesco Parello / Mathiew Yalire

    Annals of Geophysics, Vol 57, Iss

    2015  

    Abstract: Volcanoes are well known as an impressive large natural source of trace elements into the troposphere. Etna (Italy) and Nyiragongo (D.R. Congo) are two stratovolcanoes located in different geological settings, both characterized by persistent passive ... ...

    Abstract Volcanoes are well known as an impressive large natural source of trace elements into the troposphere. Etna (Italy) and Nyiragongo (D.R. Congo) are two stratovolcanoes located in different geological settings, both characterized by persistent passive degassing from their summit craters. Here, we present some results on trace element composition in volcanic plume emissions, atmospheric bulk deposition (rainwater) and their uptake by the surrounding vegetation, with the aim to compare and identify differences and similarities between these two volcanoes. Volcanic emissions were sampled by using active filter-packs for acid gases (sulfur and halogens) and specific teflon filters for particulates (major and trace elements). The environmental impact of the volcanogenic deposition in the area surrounding of the crater rims was investigated by using different sampling techniques: bulk rain collectors’ gauges were used to collect atmospheric bulk deposition, and biomonitoring was carried out to collect gases and particulates by using endemic plant species. The estimates of the trace element fluxes confirm that Etna and Nyiragongo are large sources of metals into the atmosphere, especially considering their persistent state of passive degassing. The large amount of emitted trace elements is clearly reflected on the chemical composition of rainwater collected at the summit areas both for Etna and Nyiragongo. Moreover, the biomonitoring results highlight that bioaccumulation of trace elements is extremely high in the proximity of the crater rim and de- creases with the distance from the active craters.
    Keywords Trace elements ; volcanic emissions ; rainwater ; biomonitoring ; Geophysics. Cosmic physics ; QC801-809 ; Physics ; QC1-999 ; Science ; Q
    Subject code 550
    Language English
    Publishing date 2015-02-01T00:00:00Z
    Publisher Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: The SLX4 Complex Is a SUMO E3 Ligase that Impacts on Replication Stress Outcome and Genome Stability

    Guervilly, Jean-Hugues / Arato Takedachi / Charly Chawhan / Emmanuelle Despras / Filippo Rosselli / Isao Kuraoka / Patricia Kannouche / Pierre-Henri L. Gaillard / Sarah Scaglione / Valeria Naim / Yoann Lovera

    Molecular cell. 2015 Jan. 08, v. 57

    2015  

    Abstract: The SLX4 Fanconi anemia protein is a tumor suppressor that may act as a key regulator that engages the cell into specific genome maintenance pathways. Here, we show that the SLX4 complex is a SUMO E3 ligase that SUMOylates SLX4 itself and the XPF subunit ...

    Abstract The SLX4 Fanconi anemia protein is a tumor suppressor that may act as a key regulator that engages the cell into specific genome maintenance pathways. Here, we show that the SLX4 complex is a SUMO E3 ligase that SUMOylates SLX4 itself and the XPF subunit of the DNA repair/recombination XPF-ERCC1 endonuclease. This SLX4-dependent activity is mediated by a remarkably specific interaction between SLX4 and the SUMO-charged E2 conjugating enzyme UBC9 and relies not only on newly identified SUMO-interacting motifs (SIMs) in SLX4 but also on its BTB domain. In contrast to its ubiquitin-binding UBZ4 motifs, SLX4 SIMs are dispensable for its DNA interstrand crosslink repair functions. Instead, while detrimental in response to global replication stress, the SUMO E3 ligase activity of the SLX4 complex is critical to prevent mitotic catastrophe following common fragile site expression.
    Keywords DNA ; DNA repair ; Fanconi anemia ; genome ; mitosis ; tumor suppressor proteins ; ubiquitin-protein ligase
    Language English
    Dates of publication 2015-0108
    Size p. 123-137.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 1415236-8
    ISSN 1097-4164 ; 1097-2765
    ISSN (online) 1097-4164
    ISSN 1097-2765
    DOI 10.1016/j.molcel.2014.11.014
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: A genome-wide collection of Mos1 transposon insertion mutants for the C. elegans research community.

    Elodie Vallin / Joseph Gallagher / Laure Granger / Edwige Martin / Jérôme Belougne / Julien Maurizio / Yohann Duverger / Sarah Scaglione / Caroline Borrel / Elisabeth Cortier / Karima Abouzid / Maité Carre-Pierrat / Kathrin Gieseler / Laurent Ségalat / Patricia E Kuwabara / Jonathan J Ewbank

    PLoS ONE, Vol 7, Iss 2, p e

    2012  Volume 30482

    Abstract: Methods that use homologous recombination to engineer the genome of C. elegans commonly use strains carrying specific insertions of the heterologous transposon Mos1. A large collection of known Mos1 insertion alleles would therefore be of general ... ...

    Abstract Methods that use homologous recombination to engineer the genome of C. elegans commonly use strains carrying specific insertions of the heterologous transposon Mos1. A large collection of known Mos1 insertion alleles would therefore be of general interest to the C. elegans research community. We describe here the optimization of a semi-automated methodology for the construction of a substantial collection of Mos1 insertion mutant strains. At peak production, more than 5,000 strains were generated per month. These strains were then subject to molecular analysis, and more than 13,300 Mos1 insertions characterized. In addition to targeting directly more than 4,700 genes, these alleles represent the potential starting point for the engineered deletion of essentially all C. elegans genes and the modification of more than 40% of them. This collection of mutants, generated under the auspices of the European NEMAGENETAG consortium, is publicly available and represents an important research resource.
    Keywords Medicine ; R ; Science ; Q
    Subject code 001
    Language English
    Publishing date 2012-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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