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Article ; Online: Domain analysis of Drosophila Blimp-1 reveals the importance of its repression function and instability in determining pupation timing.

Sarhan, Moustafa / Miyagawa, Koichi / Ueda, Hitoshi

Genes to cells : devoted to molecular & cellular mechanisms

2023 Volume 28, Issue 5, Page(s) 338–347

Abstract: The PRDM family transcription repressor Blimp-1 is present in almost all multicellular organisms and plays important roles in various developmental processes. This factor has several conserved motifs among different species, but the function of each ... ...

Abstract	The PRDM family transcription repressor Blimp-1 is present in almost all multicellular organisms and plays important roles in various developmental processes. This factor has several conserved motifs among different species, but the function of each motif is unclear. Drosophila Blimp-1 plays an important role in determining pupation timing by acting as an unstable transcriptional repressor of the βftz-f1 gene. Thus, Drosophila provides a good system for analyzing the molecular and biological functions of each region in Blimp-1. Various Blimp-1 mutants carrying deletions at the conserved motifs were induced under the control of the heat shock promoter in prepupae, and the expression patterns of βFTZ-F1 and Blimp-1 and pupation timing were observed. The results showed that the regions with strong and weak repressor functions exist within the proline-rich middle section of the factor and near the N-terminal conserved motif, respectively. Rapid degradation was supported by multiple regions that were mainly located in a large proline-rich region. Results revealed that pupation timing was affected by the repression ability and stability of Blimp-1. This suggests that both the repression function and instability of Blimp-1 are indispensable for the precise determination of pupation timing.
MeSH term(s)	Animals ; DNA-Binding Proteins/metabolism ; Drosophila/genetics ; Drosophila/metabolism ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Promoter Regions, Genetic ; Repressor Proteins/metabolism
Chemical Substances	Blimp-1 protein, Drosophila ; DNA-Binding Proteins ; Drosophila Proteins ; Repressor Proteins ; nuclear hormone receptor FTZ-F1, Drosophila
Language	English
Publishing date	2023-03-06
Publishing country	England
Document type	Journal Article
ZDB-ID	1330000-3
ISSN	1365-2443 ; 1356-9597
ISSN (online)	1365-2443
ISSN	1356-9597
DOI	10.1111/gtc.13020
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Article: Topical application of Aloe gel and/or olive oil combination promotes the wound healing properties of streptozotocin-induced diabetic rats

Massoud, Diaa / Fouda, Maged M. A. / Sarhan, Moustafa / Salama, Shaimaa Gamal / Khalifa, Heba Saied

Environmental science and pollution research. 2022 Aug., v. 29, no. 39

2022

Abstract: Diabetic wounds are characterized by a delayed closure rate due to the excess inflammation and the inhibition of angiogenesis. Natural products derived from Aloe vera have shown great promise due to their healing magnificent properties. Olive oil is ... ...

Abstract	Diabetic wounds are characterized by a delayed closure rate due to the excess inflammation and the inhibition of angiogenesis. Natural products derived from Aloe vera have shown great promise due to their healing magnificent properties. Olive oil is another natural product with anti-microbial and anti-inflammatory properties that may contribute to the healing process. In the present investigation, we tried to evaluate the efficacy of topical application of Aloe gel and/or olive oil in the enhancement of diabetic wounds using histological and immunohistochemical analysis. Excisional wounds were created on the back skin of streptozotocin-induced diabetic rats. Topical treatments of Aloe gel and/or olive oil were applied separately and in a combination (AVO) daily for experimental groups. Macroscopic and microscopic observations of the excision wounds were monitored at time intervals (3, 6, 9, 14 days) post-wounding. Macroscopic observations of the AVO group exhibited almost complete healing at day 14, while other groups were still in progress. Similarly, immunohistochemical analysis of the AVO group showed a mild expression pattern of NF-κB.. While, the cell proliferation (Ki-67), and angiogenesis (CD34) markers were upregulated. Conclusively, the obtained results showed that the AVO combination effectively improved the healing process in diabetic excisional wounds with significant differences in the healing kinetics compared to wounds that received Aloe gel or olive oil separately.
Keywords	Aloe vera ; angiogenesis ; cell proliferation ; excision ; gels ; immunohistochemistry ; inflammation ; olive oil ; pollution ; research ; topical application
Language	English
Dates of publication	2022-08
Size	p. 59727-59735.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-022-20100-9
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Article ; Online: Potent virucidal activity of honeybee "Apis mellifera" venom against Hepatitis C Virus.

Sarhan, Moustafa / El-Bitar, Alaa M H / Hotta, Hak

Toxicon : official journal of the International Society on Toxinology

2020 Volume 188, Page(s) 55–64

Abstract: Hepatitis C virus (HCV) is a global viral widespread without an available vaccine to prevent infection. HCV infection can cause serious liver diseases such as hepatocellular carcinoma (HCC). Current treatment of HCV infection depends on the FDA approved ... ...

Abstract	Hepatitis C virus (HCV) is a global viral widespread without an available vaccine to prevent infection. HCV infection can cause serious liver diseases such as hepatocellular carcinoma (HCC). Current treatment of HCV infection depends on the FDA approved direct-acting antivirals (DAAs) which have side effects and expensive. Thus, development of a novel, more efficient, along with affordable pricing anti-HCV agents is still required. The purpose of the present study is to evaluate the antiviral effects of bee venom (BV) from the honeybee Apis mellifera on the HCV replication life cycle. The crude venom and its components were examined for their anti-HCV activities using Huh7it-1 cultured cells and the JFH1 strain of HCV genotype 2a. Results revealed that BV inhibited HCV infection with 50% inhibitory concentration (IC
MeSH term(s)	Animals ; Antiviral Agents/pharmacology ; Bee Venoms/pharmacology ; Bees ; Carcinoma, Hepatocellular ; Cell Line ; Hepacivirus ; Hepatitis C ; Hepatitis C, Chronic ; Liver Neoplasms ; Melitten ; Peptides
Chemical Substances	Antiviral Agents ; Bee Venoms ; Peptides ; Melitten (20449-79-0) ; mast cell degranulating peptide (32908-73-9)
Language	English
Publishing date	2020-10-14
Publishing country	England
Document type	Journal Article
ZDB-ID	204479-1
ISSN	1879-3150 ; 0041-0101
ISSN (online)	1879-3150
ISSN	0041-0101
DOI	10.1016/j.toxicon.2020.10.014
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Article ; Online: Topical application of Aloe gel and/or olive oil combination promotes the wound healing properties of streptozotocin-induced diabetic rats.

Massoud, Diaa / Fouda, Maged M A / Sarhan, Moustafa / Salama, Shaimaa Gamal / Khalifa, Heba Saied

Environmental science and pollution research international

2022 Volume 29, Issue 39, Page(s) 59727–59735

Abstract	Diabetic wounds are characterized by a delayed closure rate due to the excess inflammation and the inhibition of angiogenesis. Natural products derived from Aloe vera have shown great promise due to their healing magnificent properties. Olive oil is another natural product with anti-microbial and anti-inflammatory properties that may contribute to the healing process. In the present investigation, we tried to evaluate the efficacy of topical application of Aloe gel and/or olive oil in the enhancement of diabetic wounds using histological and immunohistochemical analysis. Excisional wounds were created on the back skin of streptozotocin-induced diabetic rats. Topical treatments of Aloe gel and/or olive oil were applied separately and in a combination (AVO) daily for experimental groups. Macroscopic and microscopic observations of the excision wounds were monitored at time intervals (3, 6, 9, 14 days) post-wounding. Macroscopic observations of the AVO group exhibited almost complete healing at day 14, while other groups were still in progress. Similarly, immunohistochemical analysis of the AVO group showed a mild expression pattern of NF-κB.. While, the cell proliferation (Ki-67), and angiogenesis (CD34) markers were upregulated. Conclusively, the obtained results showed that the AVO combination effectively improved the healing process in diabetic excisional wounds with significant differences in the healing kinetics compared to wounds that received Aloe gel or olive oil separately.
MeSH term(s)	Aloe ; Animals ; Diabetes Mellitus, Experimental ; Olive Oil/pharmacology ; Rats ; Streptozocin/pharmacology ; Wound Healing
Chemical Substances	Olive Oil ; Streptozocin (5W494URQ81)
Language	English
Publishing date	2022-04-08
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-022-20100-9
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Article ; Online: DNA barcoding supports life stages of wrasse fish, Novaculichthys taeniourus (Family Labridae) in the Hurghada Red Sea, Egypt

Afifi, Moharam Adel Mohamed / Sarhan, Moustafa / Khalaf-Allah, Hassan M.M. / Alabssawy, Ahmed Nasr / Abbas, Mahmoud Mahrous M. / Abdel-Hamid, Fouad / El-Tabakh, Mohamed Ahmed Mohamed

Zoologischer Anzeiger. 2023 May, v. 304 p.84-93

2023

Abstract: Family Labridae considered diverse group of the fish that come in a variety of body forms, sizes, colors, and environments. Due to the great overlap between the species of this family, we find it very difficult to classify them using traditional methods. ...

Abstract	Family Labridae considered diverse group of the fish that come in a variety of body forms, sizes, colors, and environments. Due to the great overlap between the species of this family, we find it very difficult to classify them using traditional methods. The goal of current study is to look at intra-specific development in Novaculichthys taeniourus, family Labridae. To assess whether the differences of morphological aspects are related to age differences, we conducted morphometric and DNA barcoding analyses. The majority of related body metrics in both sub-adult and adult were development, according to the findings. However, there is no significant changes in cytochrome oxidase subunit I (COI) sequence were found between sub-adult and adult specimens studied, indicating that the differences of phenotypic are related to development rather than differences of the species.
Keywords	DNA barcoding ; Labridae ; adults ; cytochrome-c oxidase ; fish ; morphometry ; phenotype ; Egypt ; Red Sea ; Life stages ; Intra-specific ; Cytochrome oxidase subunit I ; Sub-adult and adult
Language	English
Dates of publication	2023-05
Size	p. 84-93.
Publishing place	Elsevier GmbH
Document type	Article ; Online
ZDB-ID	398-0
ISSN	1873-2674 ; 0044-5231
ISSN (online)	1873-2674
ISSN	0044-5231
DOI	10.1016/j.jcz.2023.03.005
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Article: Proteomic analysis of Red Sea

Fouda, Maged M A / Abdel-Wahab, Mohammed / Mohammadien, Amal / Germoush, Mousa O / Sarhan, Moustafa

The journal of venomous animals and toxins including tropical diseases

2021 Volume 27, Page(s) e20210023

Abstract: Background: Diverse and unique bioactive neurotoxins known as conopeptides or conotoxins are produced by venomous marine cone snails. Currently, these small and stable molecules are of great importance as research tools and platforms for discovering new ...

Abstract	Background: Diverse and unique bioactive neurotoxins known as conopeptides or conotoxins are produced by venomous marine cone snails. Currently, these small and stable molecules are of great importance as research tools and platforms for discovering new drugs and therapeutics. Therefore, the characterization of Methods: In this study, we used bioanalytical techniques to determine the venom profile and emphasize the functional composition of conopeptides in Results: The proteomic analysis revealed that 84.0% of the venom proteins were between 500 and 4,000 Da, and 16.0% were > 4,000 Da. In Conclusion: Our study showed that the composition of the
Language	English
Publishing date	2021-10-18
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2031021-3
ISSN	1678-9199
ISSN	1678-9199
DOI	10.1590/1678-9199-JVATITD-2021-0023
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Article: Virucidal activity of oriental hornet

Sarhan, Moustafa / El-Bitar, Alaa M H / Mohammadein, Amaal / Elshehaby, Mohammed / Hotta, Hak

The journal of venomous animals and toxins including tropical diseases

2021 Volume 27, Page(s) e20210039

Abstract: Background: Hepatitis C virus (HCV) infection is a major worldwide health problem that can cause liver fibrosis and hepatocellular carcinoma (HCC). The clinical treatment of HCV infection mainly relies on the use of direct-acting antivirals (DAAs) that ... ...

Abstract	Background: Hepatitis C virus (HCV) infection is a major worldwide health problem that can cause liver fibrosis and hepatocellular carcinoma (HCC). The clinical treatment of HCV infection mainly relies on the use of direct-acting antivirals (DAAs) that are usually expensive and have side effects. Therefore, achieving the discovery of more successful agents is always urgent. In this context, antiviral compounds that inhibit viral infections and disease progression with important therapeutic activities have been identified in animal venoms including arthropod toxins. This indicates that arthropod venoms represent a good natural source of promising candidates for new antivirals. Methods: The antiviral activity of the wasp venom (WV), isolated from the Oriental hornet ( Results: The results revealed that WV inhibited HCV infectivity with 50% inhibitory concentration (IC Conclusion: WV can inhibit the entry stage of HCV infection at non-cytotoxic concentrations. Therefore, it could be considered a potential candidate for characterization of natural anti-HCV agents targeting the entry step.
Language	English
Publishing date	2021-11-19
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2031021-3
ISSN	1678-9199
ISSN	1678-9199
DOI	10.1590/1678-9199-JVATITD-2021-0039
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Article: Potent virucidal activity of honeybee “Apis mellifera” venom against Hepatitis C Virus

Sarhan, Moustafa / El-Bitar, Alaa M.H / Hotta, Hak

Toxicon. 2020 Dec., v. 188

2020

Abstract	Hepatitis C virus (HCV) is a global viral widespread without an available vaccine to prevent infection. HCV infection can cause serious liver diseases such as hepatocellular carcinoma (HCC). Current treatment of HCV infection depends on the FDA approved direct-acting antivirals (DAAs) which have side effects and expensive. Thus, development of a novel, more efficient, along with affordable pricing anti-HCV agents is still required. The purpose of the present study is to evaluate the antiviral effects of bee venom (BV) from the honeybee Apis mellifera on the HCV replication life cycle. The crude venom and its components were examined for their anti-HCV activities using Huh7it-1 cultured cells and the JFH1 strain of HCV genotype 2a. Results revealed that BV inhibited HCV infection with 50% inhibitory concentration (IC₅₀) of 0.05 ng/ml, while the 50% cytotoxic concentration (CC₅₀) being 20,000 ng/ml. The venom directly blocked HCV/cell entry by acting on virus particles in a dose dependent manner, whereas no interference on the host cells. Furthermore, venom showed no inhibitory effect on HCV replication and release. Interestingly, none of the main BV components including the mast cell degranulating peptide (MCD), mpamin, or the small peptides melittin (MLT) showed anti-HCV activity up to 5 μg/ml. In conclusion, these results suggest that BV has a direct virucidal activity against HCV and may exert its antiviral effect through a non-common peptide(s) or toxin complex within the crude venom. Therefore, the crude BV can be considered as a promising candidate for characterization and development of new and natural anti-HCV therapeutic agents.
Keywords	Apis mellifera ; Hepatitis C virus ; antiviral agents ; antiviral properties ; bee venoms ; cytotoxicity ; dose response ; genotype ; hepatoma ; honey bees ; inhibitory concentration 50 ; liver ; mast cells ; melittin ; peptides ; therapeutics ; toxins ; vaccines ; viruses
Language	English
Dates of publication	2020-12
Size	p. 55-64.
Publishing place	Elsevier Ltd
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	204479-1
ISSN	1879-3150 ; 0041-0101
ISSN (online)	1879-3150
ISSN	0041-0101
DOI	10.1016/j.toxicon.2020.10.014
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Isolation and Characterization of Insecticidal Toxins from the Venom of the North African Scorpion,

Yoshimoto, Yusuke / Miyashita, Masahiro / Abdel-Wahab, Mohammed / Sarhan, Moustafa / Nakagawa, Yoshiaki / Miyagawa, Hisashi

Toxins

2019 Volume 11, Issue 4

Abstract: Various bioactive peptides have been identified in scorpion venom, but there are many scorpion species whose venom has not been investigated. In this study, we characterized venom components of the North African scorpion, ...

Abstract	Various bioactive peptides have been identified in scorpion venom, but there are many scorpion species whose venom has not been investigated. In this study, we characterized venom components of the North African scorpion,
MeSH term(s)	Amino Acid Sequence ; Animals ; Chromatography, High Pressure Liquid ; Gryllidae/drug effects ; Insecticides/chemistry ; Insecticides/isolation & purification ; Insecticides/toxicity ; Lethal Dose 50 ; Peptides/chemistry ; Peptides/isolation & purification ; Peptides/toxicity ; Scorpion Venoms/chemistry ; Scorpions ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Tandem Mass Spectrometry
Chemical Substances	Insecticides ; Peptides ; Scorpion Venoms
Language	English
Publishing date	2019-04-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2518395-3
ISSN	2072-6651 ; 2072-6651
ISSN (online)	2072-6651
ISSN	2072-6651
DOI	10.3390/toxins11040236
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Occurrence of Penaeus aztecus, Ives, 1891 (Crustacea: Decapoda: Penaeidae) in the coastal water of Alexandria, Egypt

El-Deeb, Rabab S / Sarhan, Moustafa / Khafage, Amal R / Abdel Razek, Fatma A / Abdel-Wahab, Mohammed / Omar, Hamdy A

National Institute of Oceanography and Fisheries Egyptian Journal of Aquatic Research (Online). 2020 Sept., v. 46, no. 3

2020

Abstract: A sample of 347 specimens of the northern brown shrimp Penaeus aztecus, a western Atlantic species, was collected from July 2019 to June 2020 by local bottom trawlers off two fishing areas, Abu-Qir Bay and Ma’deya, east of Alexandria Coast, Egypt. The ... ...

Abstract	A sample of 347 specimens of the northern brown shrimp Penaeus aztecus, a western Atlantic species, was collected from July 2019 to June 2020 by local bottom trawlers off two fishing areas, Abu-Qir Bay and Ma’deya, east of Alexandria Coast, Egypt. The species identification was confirmed by morphological and genetic characters. Some aspects of the species’ morphometry and its monthly abundance were briefly discussed. The collected adults were observed from May to November with higher percentage of occurrence in August (29% of total shrimp catch). Sex ratio was 1: 1.2 (F: M). The total length of the adult specimen ranged from 91 mm to 210 mm. COI sequences gave a maximum identity of 99.56% with P. aztecus from Texas and USA, and 99.44% with species from the Eastern Mediterranean. The genetic distance among the studied species from Egypt, USA and the Eastern Mediterranean ranged from 0.2 to 0.7%. The results of this study suggest that P. aztecus may become a new resource for coastal Mediterranean shrimp fisheries.
Keywords	Farfantepenaeus aztecus ; adults ; coastal water ; coasts ; fishing boats ; genetic distance ; morphometry ; sex ratio ; shrimp ; shrimp fisheries ; species identification ; Egypt ; Texas
Language	English
Dates of publication	2020-09
Size	p. 303-309.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2434122-8
ISSN	1110-0354 ; 1687-4285
ISSN (online)	1110-0354
ISSN	1687-4285
DOI	10.1016/j.ejar.2020.08.004
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