Article: Human immunodeficiency virus type 1 capsid protein is a substrate of the retroviral proteinase while integrase is resistant toward proteolysis.
2003 Volume 310, Issue 1, Page(s) 16–23
Abstract: The capsid protein of human immunodeficiency virus type 1 was observed to undergo proteolytic cleavage in vitro when viral lysate was incubated in the presence of dithiothreitol at acidic pH. Purified HIV-1 capsid protein was also found to be a substrate ...
| Abstract | The capsid protein of human immunodeficiency virus type 1 was observed to undergo proteolytic cleavage in vitro when viral lysate was incubated in the presence of dithiothreitol at acidic pH. Purified HIV-1 capsid protein was also found to be a substrate of the viral proteinase in a pH-dependent manner; acidic pH (<7) was necessary for cleavage, and decreasing the pH toward 4 increased the degree of processing. Based on N-terminal sequencing of the cleavage products, the capsid protein was found to be cleaved at two sites, between residues 77 and 78 as well as between residues 189 and 190. Oligopeptides representing these cleavage sites were also cleaved at the expected peptide bonds. The presence of cyclophilin A decreased the degree of capsid protein processing. Unlike the capsid protein, integrase was found to be resistant toward proteolysis in good agreement with its presence in the preintegration complex. |
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| MeSH term(s) | Capsid Proteins/metabolism ; Cyclophilin A/pharmacology ; HIV Integrase/metabolism ; HIV Protease/metabolism ; HIV-1/metabolism ; Hydrogen-Ion Concentration |
| Chemical Substances | Capsid Proteins ; HIV Integrase (EC 2.7.7.-) ; HIV Protease (EC 3.4.23.-) ; Cyclophilin A (EC 5.2.1.-) |
| Language | English |
| Publishing date | 2003-05-25 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S. |
| ZDB-ID | 200425-2 |
| ISSN | 1096-0341 ; 0042-6822 |
| ISSN (online) | 1096-0341 |
| ISSN | 0042-6822 |
| DOI | 10.1016/s0042-6822(03)00074-6 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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