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  1. Article ; Online: Relationship of body mass index to percent body fat determined by deuterium isotopic dilution and impedancemetry among Tunisian schoolchildren.

    Houda, Ben Jemaa / Sarra, Khlifi / Karmous, Inchirah / Henda, Jamoussi / Khalid, El Kari / Hassan, Aguenaou / Abdallah, Aouidet / Amani, Mankaï

    African health sciences

    2024  Volume 23, Issue 3, Page(s) 664–671

    Abstract: Objectives: This study aims to evaluate the relation of body mass index (BMI) to fat mass among children by two techniques impedancemetry and deuterium oxide dilution (D: Methods: This study was carried out in 156 schoolchildren aged between 8 and 11 ...

    Abstract Objectives: This study aims to evaluate the relation of body mass index (BMI) to fat mass among children by two techniques impedancemetry and deuterium oxide dilution (D
    Methods: This study was carried out in 156 schoolchildren aged between 8 and 11 years. The children received interrogation specifying lifestyle and food habits. Body composition was determined using the impedancemetry and D2O technique.
    Results: The results showed a difference between the percentage of obese and overweight children according to BMIZ classification (30.1%), bioelectrical impedance method (14.7%) and D
    Conclusion: This study has demonstrated that the percentage of overweight and obesity varied according the methods used. Further development of body composition methods is needed in children for the real determination of the obesity prevalence and therefore a better monitoring of this public health problem.
    MeSH term(s) Male ; Female ; Child ; Humans ; Body Mass Index ; Deuterium ; Overweight/diagnosis ; Overweight/epidemiology ; Adipose Tissue ; Body Composition ; Obesity/diagnosis ; Obesity/epidemiology
    Chemical Substances Deuterium (AR09D82C7G)
    Language English
    Publishing date 2024-02-03
    Publishing country Uganda
    Document type Journal Article
    ZDB-ID 2240308-5
    ISSN 1729-0503 ; 1680-6905
    ISSN (online) 1729-0503
    ISSN 1680-6905
    DOI 10.4314/ahs.v23i3.77
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  2. Article: Antidiabetic and Antioxidant Effects of Apple Cider Vinegar on Normal and Streptozotocin-Induced Diabetic Rats.

    Hmad Halima, Ben / Sarra, Khlifi / Jemaa Houda, Ben / Sonia, Gara / Abdallah, Aouidet

    International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition

    2019  Volume 88, Issue 5-6, Page(s) 223–233

    Abstract: Diabetes mellitus (DM) is a globally growing health problem and one of the most prevalent endocrine disorders worldwide. Chronic hyperglycemia status favors the manifestation of oxidative stress by increasing the production of reactive oxygen species and/ ...

    Abstract Diabetes mellitus (DM) is a globally growing health problem and one of the most prevalent endocrine disorders worldwide. Chronic hyperglycemia status favors the manifestation of oxidative stress by increasing the production of reactive oxygen species and/or by reducing the antioxidant defense system activity. In this study, the beneficial effects of apple cider vinegar (ACV) supplementation on blood glucose level, hepatic and renal toxicity and antioxidant enzyme activities in normal and diabetic rats were investigated. Four groups (8 animals/group) of male
    MeSH term(s) Animals ; Antioxidants/pharmacology ; Diabetes Mellitus, Experimental ; Hypoglycemic Agents/metabolism ; Hypoglycemic Agents/pharmacology ; Lipid Peroxidation ; Liver/chemistry ; Liver/metabolism ; Male ; Malus ; Oxidative Stress ; Rats ; Rats, Wistar ; Streptozocin/administration & dosage ; Streptozocin/chemistry
    Chemical Substances Antioxidants ; Hypoglycemic Agents ; Streptozocin (5W494URQ81)
    Language English
    Publishing date 2019-05-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 120692-8
    ISSN 0300-9831
    ISSN 0300-9831
    DOI 10.1024/0300-9831/a000246
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  3. Article ; Online: Apple Cider Vinegar Attenuates Oxidative Stress and Reduces the Risk of Obesity in High-Fat-Fed Male Wistar Rats.

    Halima, Ben Hmad / Sonia, Gara / Sarra, Khlifi / Houda, Ben Jemaa / Fethi, Ben Slama / Abdallah, Aouidet

    Journal of medicinal food

    2017  

    Abstract: Metabolic syndrome is a serious consequence of obesity characterized by increased cardiovascular risk factors such as hypertension, dyslipidemia, and glucose intolerance. While diets enriched with natural antioxidants showed beneficial effects on ... ...

    Abstract Metabolic syndrome is a serious consequence of obesity characterized by increased cardiovascular risk factors such as hypertension, dyslipidemia, and glucose intolerance. While diets enriched with natural antioxidants showed beneficial effects on oxidative stress, blood pressure, and serum lipid composition, diet supplementation with synthetic antioxidants showed contradictive results. Thus, we tested, in this study, whether a daily dosage of Apple Cider Vinegar (ACV) would affect cardiovascular risk factor associated with obesity in high-fat diet (HFD)-induced hyperlipidemic Wistar rats. Obese rats showed increased serum total cholesterol, triglyceride, low-density lipoprotein-cholesterol (LDL-C), very low density lipoprotein (VLDL) and atherogenic index after 6 and 9 weeks of being fed an HFD. Importantly, ACV ameliorated all of these parameters significantly. Oxidative stress already developed after 6 weeks of HFD and was significantly reduced by daily doses of ACV. Oral administration of ACV normalized various biochemical and metabolic changes since it exhibited a very significant (P < .001) reduction in malondialdehyde levels, whereas an increase in thiol group concentrations and antioxidant status (superoxide dismutase [SOD], glutathione peroxidase [GPx], and catalase [CAT] activities and vitamin E concentrations). In addition, a modulation in trace element levels was observed when compared with HFD groups. These findings suggested that HFD alters the oxidant-antioxidant balance, as evidenced by a reduction in the antioxidant enzyme activities and vitamin E level, and enhanced lipid peroxidation. ACV can be beneficial for the suppression of obesity-induced oxidative stress in HFD rats through the modulating antioxidant defense system and reduces the risk of obesity-associated diseases by preventing the atherogenic risk.
    Language English
    Publishing date 2017-11-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1427365-2
    ISSN 1557-7600 ; 1096-620X
    ISSN (online) 1557-7600
    ISSN 1096-620X
    DOI 10.1089/jmf.2017.0039
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  4. Article ; Online: Antioxidant, Antidiabetic and Antihyperlipidemic Effects of Trigonella foenum - graecum Seeds

    Sarra Khlifi / Houda Ben Jemaa / Halima Ben Hmad / Hajer Abaza / Inchirah Karmous / Abdelmajid Abid / Anis Benzarti / Jalila Elati / Abdallah Aouidet

    International Journal of Pharmacology, Vol 12, Iss 4, Pp 394-

    2016  Volume 400

    Abstract: Fenugreek ( Trigonella foenum - graecum ) seeds have been known as a traditional plant treatment for diabetes. The aims of this study were to determine the antioxidant activity of fenugreek seeds and to evaluate the antidiabetic and antihyperlipidemic ... ...

    Abstract Fenugreek ( Trigonella foenum - graecum ) seeds have been known as a traditional plant treatment for diabetes. The aims of this study were to determine the antioxidant activity of fenugreek seeds and to evaluate the antidiabetic and antihyperlipidemic effect of fenugreek seeds intake on diabetic patients. Sixty newly diagnosed diabetic patients were recruited for this study and have received two doses of fenugreek seeds (2.5 and 5 g) for 4 weeks. Phytochemicals analysis and antioxidant activity of Trigonella foenum - graecum seeds methanolic extract were also studied. The chemical analysis of fenugreek seeds exhibited a high level in total polyphenols (9.5 GAE g/g VM) and flavonoids (3.7 mg RE/g VM). Fenugreek seeds exertan important DPPH scavenging ability in terms of Trolox Equivalents (TE) with 1.03±0.008 mg TE/g VM and also exhibited a high antioxidant potential according to ABTS test. In Tunisian type 2 diabetic patients, the administration of fenugreek seeds (2.5 and 5 g) for 4 weeks improved blood glucose level in dose-dependent. Total cholesterol and triglycerides rate were significantly decreased by the dose of 5 g Trigonella foenum - graecum seeds. Moreover, daily oral intake of our treatment by diabetic subjects reduced significantly serum α-amylase activity. The present findings indicate that fenugreek seeds with abundant flavonoids and total polyphenols content may be a good adjuvant for the treatment of diabetes.
    Keywords blood glucose levels ; Hyperglycemia ; lipid profile ; triglycerides ; fenugreek ; Therapeutics. Pharmacology ; RM1-950 ; Medicine ; R
    Subject code 571
    Language English
    Publishing date 2016-01-01T00:00:00Z
    Publisher Asian Network for Scientific Information
    Document type Article ; Online
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  5. Article ; Online: Antidiabetic, Antihyperlipidemic and Antioxidant Effects of Artemisia herba alba Extract on Experimental Diabetes

    Houda Ben Jemaa / Sarra Khlifi / Halima Ben Hmed / Inchirah Karmous / Anis Benzarti / Jalila Elati / Abdallah Aouidet

    International Journal of Pharmacology, Vol 11, Iss 6, Pp 552-

    2015  Volume 560

    Abstract: Artemisia herba alba was widely used in traditional medicine for the treatment of diabetes. The objective of this study was to evaluate the antidiabetic, antihyperlipidemic and antioxidant effects of Artemisia herba alba extract on high fat diet and ... ...

    Abstract Artemisia herba alba was widely used in traditional medicine for the treatment of diabetes. The objective of this study was to evaluate the antidiabetic, antihyperlipidemic and antioxidant effects of Artemisia herba alba extract on high fat diet and diabetic models. Wistar albinos male rats were divided into four groups, the first was fed with high fat diet, the second was mimetic diabetes type II with high fat diet combined with double low dose of streptozotocin , the third was a model of type I diabetes with high dose of streptozotocin and the fourth was a control group. The ethanolic extract of Artemisia herba alba was given orally (2 g kg -1 b.wt.) and daily for a period of 8 weeks. Our results showed that Artemisia herba alba treatment reduces the blood glucose level significantly in type II diabetic group (17.50±2.34 g L -1 vs., 10.93±3.19 g L -1 ). The plasma lipoproteins HDL, LDL-cholesterol and the triglyceride levels were altered by high fat diet and also by streptozotocin injection. These levels were also reverted back to near normalcy by treatment. Lipid peroxidation was decreased significantly by Artemisia herba alba supplementation in high fat diet and diabetic groups (p<0.05). Treatment with investigational drug increases antioxidant enzymes activities (superoxide dismutase and catalase in liver and kidneys) for type I and type II diabetic groups. In conclusion, this study suggests that the Artemisia herba alba has a beneficial effect in controlling diabetes by reducing lipid peroxidation and increasing antioxidant enzymes activities, which reduces the risk of developing diabetes complications.
    Keywords antihyperlipidemic ; antioxidant ; Antidiabetic ; Artemisia herba alba ; diabetes ; Therapeutics. Pharmacology ; RM1-950 ; Medicine ; R
    Subject code 630
    Language English
    Publishing date 2015-01-01T00:00:00Z
    Publisher Asian Network for Scientific Information
    Document type Article ; Online
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  6. Article: Development and validation of impedance-based equations for the prediction of total body water and fat-free mass in children aged 8–11 years

    Ben Jemaa, Houda / Abdallah Aouidet / Amani Mankaï / Belhassen Kortobi / Dhekra Ghozzi / Fethi Ben Slama / Halima Ben Hmad / Hassan Aguenaou / Henda Jamoussi / Inchirah Karmous / Khalid El Kari / Manel Zediri / Rim Minaoui / Sarra Khlifi

    Clinical nutrition. 2019 Feb., v. 38, no. 1

    2019  

    Abstract: Accuracy of impedance analysis depends on population-specific prediction equations. The aim of this study was to propose new prediction equations to estimate body composition using impedance analysis for healthy Tunisian children and validate them using ... ...

    Abstract Accuracy of impedance analysis depends on population-specific prediction equations. The aim of this study was to propose new prediction equations to estimate body composition using impedance analysis for healthy Tunisian children and validate them using the deuterium oxide dilution (D2O) technique.Body composition was estimated by the Tanita impedance analyzer. The validity of this system was investigated in 134 school children aged between 8 and 11 years. The validity of other published equations was also tested in our sample. Total body water (TBW) and Fat-Free Mass (FFM) were determined using the D2O technique. Participants were divided equally into development (n = 67) and validation groups (n = 67) to develop prediction equation using linear regression models.The comparison between body composition obtained by Tanita system and by D2O technique illustrated a significant difference (p < 0.01). Compared to D2O technique, Tanita impedance analysis underestimates fat mass and overestimates FFM and TBW. The prediction equations for TBW and FFM were developed with sex, age, weight and resistance index as possible predictor variables. The selected equations presented the highest adjusted coefficient of determination (R2), the lowest standard error of the estimate (SEE) value and the lowest p-values. The pure error was 1.263 for the TBW equation and 1.646 for the FFM. The Bland Altman plot illustrated the good level of concordance between the TBW and FFM predicted by the new equations and measured by isotope dilution.Our study provides valid prediction equations for estimation of TBW, FFM from impedance analysis measures for Tunisian children. These equations are applicable to children aged between 8 and 11 years.
    Keywords body water ; children ; deuterium oxide ; equations ; isotopes ; lean body mass ; prediction ; regression analysis
    Language English
    Dates of publication 2019-02
    Size p. 227-233.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 604812-2
    ISSN 1532-1983 ; 0261-5614
    ISSN (online) 1532-1983
    ISSN 0261-5614
    DOI 10.1016/j.clnu.2018.01.028
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Indicators of oxidative stress in weanling and pubertal rats following exposure to nicotine via milk.

    Halima, Ben Ahmed / Sarra, Khlifi / Kais, Rtibi / Salwa, Elfazaa / Najoua, Gharbi

    Human & experimental toxicology

    2010  Volume 29, Issue 6, Page(s) 489–496

    Abstract: Nicotine, a major toxic component of tobacco, has been identified as an important risk factor for infant and children diseases. It is concentrated in breast milk and is absorbed by the infant. The purpose of the present study was to investigate the ... ...

    Abstract Nicotine, a major toxic component of tobacco, has been identified as an important risk factor for infant and children diseases. It is concentrated in breast milk and is absorbed by the infant. The purpose of the present study was to investigate the effects of maternal nicotine exposure during lactation on breast-fed rats and at the pubertal age by measuring biomarkers of oxidative stress. Particularly, a new parameter, the thiol concentration was evaluated. Two groups of lactating Wistar rats were used. For the first group, female rats were given an intraperitoenal injection of nicotine or saline (2 mg/kg per day) during lactation. For the second group, we reproduced the same process described above and then the female and male pups were separately kept after weaning without any treatment until the puberty (at 45 days of age). In the liver and lung of the offspring, we examined the malondialdehyde (MDA) level, the thiol concentration, and the activities of two antioxidant enzymes: superoxyde dismutase (SOD) and catalase (CAT). In the plasma, alanine amino transferase (ALT) and aspartate amino transferase (AST) activities were measured. For rats aged 21 days, the treatment significantly reduced the thiol concentration, SOD, and CAT activities but increased MDA level, AST, and ALT activities. For rats aged 45 days, the males and females did not react the same way. In fact, the males were more affected. These results indicate that maternal nicotine exposure during the lactation period induces oxidative stress in the liver and lung of lactating offspring, which is maintained until the puberty, especially for the male rats.
    MeSH term(s) Aging ; Animals ; Biomarkers/blood ; Biomarkers/metabolism ; Catalase/metabolism ; Female ; Lactation ; Lipid Peroxidation/drug effects ; Liver/drug effects ; Liver/metabolism ; Liver Function Tests ; Lung/drug effects ; Lung/metabolism ; Male ; Malondialdehyde/metabolism ; Maternal Exposure/adverse effects ; Nicotine/toxicity ; Oxidative Stress/drug effects ; Rats ; Rats, Wistar ; Sex Characteristics ; Sexual Maturation ; Sulfhydryl Compounds/metabolism ; Superoxide Dismutase/metabolism ; Weight Gain/drug effects
    Chemical Substances Biomarkers ; Sulfhydryl Compounds ; Malondialdehyde (4Y8F71G49Q) ; Nicotine (6M3C89ZY6R) ; Catalase (EC 1.11.1.6) ; Superoxide Dismutase (EC 1.15.1.1)
    Language English
    Publishing date 2010-06
    Publishing country England
    Document type Comparative Study ; Journal Article
    ZDB-ID 1027454-6
    ISSN 1477-0903 ; 0144-5952 ; 0960-3271
    ISSN (online) 1477-0903
    ISSN 0144-5952 ; 0960-3271
    DOI 10.1177/0960327109354440
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