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  1. Article: Recipe for pituitary organoids.

    Kano, Mayuko / Sasaki, Hiroo / Miwata, Tsutomu / Suga, Hidetaka

    Frontiers in endocrinology

    2023  Volume 13, Page(s) 1025825

    Abstract: Generation of a variety of organs and tissues from human pluripotent stem cells (hPSCs) has been ... ...

    Abstract Generation of a variety of organs and tissues from human pluripotent stem cells (hPSCs) has been attempted
    MeSH term(s) Animals ; Humans ; Mice ; Cell Differentiation ; Hedgehog Proteins ; Hypopituitarism ; Organoids ; Pituitary Gland
    Chemical Substances Hedgehog Proteins
    Language English
    Publishing date 2023-01-19
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2592084-4
    ISSN 1664-2392
    ISSN 1664-2392
    DOI 10.3389/fendo.2022.1025825
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Complex aneurysm formation in the proximal segment of the posterior cerebral artery: a report of two cases.

    Sasaki, Hiroo / Takasu, Syuntaro / Seki, Yukio

    Nagoya journal of medical science

    2023  Volume 85, Issue 2, Page(s) 350–356

    Abstract: Posterior cerebral artery (PCA) aneurysms are rare and often fusiform. We describe two cases of complex proximal PCA aneurysm in two women in their 60's, which probably resulted from segmental arterial degeneration. Both presented with subarachnoid ... ...

    Abstract Posterior cerebral artery (PCA) aneurysms are rare and often fusiform. We describe two cases of complex proximal PCA aneurysm in two women in their 60's, which probably resulted from segmental arterial degeneration. Both presented with subarachnoid hemorrhage and had common angiographic and intraoperative findings: tortuous configuration of the affected P1 segment, whitish or yellowish appearance of a portion of the lesion, lesion calcification, and multiple aneurysms in the segment. Interestingly, no significant atherosclerotic changes were noted in other cerebral arteries. The ruptured aneurysm could be successfully trapped, with superficial temporal artery (STA)-PCA bypass in one and without bypass in the other, and both patients recovered well. As complex aneurysm formation in the cases described here are probably related to proximal PCA segmental degeneration, we recommend trapping the lesion, with or without STA-PCA bypass, depending on the size and patency of the posterior communicating artery.
    MeSH term(s) Humans ; Female ; Posterior Cerebral Artery/diagnostic imaging ; Posterior Cerebral Artery/surgery ; Posterior Cerebral Artery/pathology ; Cerebral Revascularization/methods ; Intracranial Aneurysm/diagnostic imaging ; Intracranial Aneurysm/surgery ; Subarachnoid Hemorrhage/diagnostic imaging ; Subarachnoid Hemorrhage/etiology ; Subarachnoid Hemorrhage/surgery ; Temporal Arteries/surgery ; Aneurysm, Ruptured/diagnostic imaging ; Aneurysm, Ruptured/surgery ; Cerebral Angiography
    Language English
    Publishing date 2023-06-13
    Publishing country Japan
    Document type Case Reports
    ZDB-ID 193148-9
    ISSN 2186-3326 ; 0027-7622
    ISSN (online) 2186-3326
    ISSN 0027-7622
    DOI 10.18999/nagjms.85.2.350
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Modified Shoelace Dural Closure with Collagen Matrix in Extended Transsphenoidal Surgery.

    Nagata, Yuichi / Takeuchi, Kazuhito / Sasaki, Hiroo / Mizuno, Akihiro / Harada, Hideyuki / Tanahashi, Kuniaki / Araki, Yoshio / Saito, Ryuta

    Neurologia medico-chirurgica

    2022  Volume 62, Issue 4, Page(s) 203–208

    Abstract: Extended endonasal transsphenoidal surgery (eTSS) offers a wide surgical field for various parasellar lesions; however, intraoperative high-flow cerebrospinal fluid (CSF) leakage is inevitable. Therefore, secure sellar reconstruction methods are ... ...

    Abstract Extended endonasal transsphenoidal surgery (eTSS) offers a wide surgical field for various parasellar lesions; however, intraoperative high-flow cerebrospinal fluid (CSF) leakage is inevitable. Therefore, secure sellar reconstruction methods are essential to prevent postoperative CSF leakage. Although collagen matrix has been applied for dural reconstruction in neurosurgery, its suitability for application in extended eTSS remains unclear. Eighteen patients underwent modified shoelace dural closure using collagen matrix after lesionectomy via extended eTSS. In this technique, a collagen matrix, which was placed subdurally (inlay graft), was continuously sutured with both open dural edges like a shoelace. Then, another collagen matrix was placed epidurally (onlay graft), and rigid reconstruction was performed using the septal bone and a resorbable fixation mesh. Postoperative CSF leakage did not occur in 17 patients but did occur in 1 patient with tuberculum sellae meningioma. In this case, the CSF leakage point was detected just around the area between the coagulated dura and the adjacent collagen matrix. The collagen matrix harvested from this area was pathologically examined; neovascularization and fibroblastic infiltration into the collagen matrix were not detected. On the other hand, neovascularization and fibroblast infiltration into the collagen matrix were apparent on the surface of the collagen matrix harvested from the non-CSF leakage area. Our novel dural closure technique using collagen matrix could be an effective option for sellar reconstruction in extended eTSS; however, it should be applied in patients in whom normal dural edges are preserved.
    MeSH term(s) Cerebrospinal Fluid Leak/etiology ; Cerebrospinal Fluid Leak/prevention & control ; Collagen/therapeutic use ; Dura Mater/surgery ; Humans ; Meningeal Neoplasms/pathology ; Postoperative Complications/etiology ; Postoperative Complications/prevention & control
    Chemical Substances Collagen (9007-34-5)
    Language English
    Publishing date 2022-03-16
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 604061-5
    ISSN 1349-8029 ; 0470-8105
    ISSN (online) 1349-8029
    ISSN 0470-8105
    DOI 10.2176/jns-nmc.2021-0355
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: The importance of preserving the superior hypophyseal artery infundibular branch in craniopharyngioma surgery.

    Sasaki, Hiroo / Takeuchi, Kazuhito / Nagata, Yuichi / Tanahashi, Kuniaki / Mizuno, Akihiro / Harada, Hideyuki / Kishida, Yugo / Watanabe, Tadashi / Nagatani, Tetsuya / Saito, Ryuta

    Acta neurochirurgica

    2022  Volume 165, Issue 3, Page(s) 667–675

    Abstract: Purpose: Postoperative pituitary dysfunction, a critical problem in the treatment of craniopharyngiomas, can occur even when the pituitary stalk is preserved. We hypothesized that compromise of the primary superior hypophyseal artery (pSHA) might be ... ...

    Abstract Purpose: Postoperative pituitary dysfunction, a critical problem in the treatment of craniopharyngiomas, can occur even when the pituitary stalk is preserved. We hypothesized that compromise of the primary superior hypophyseal artery (pSHA) might be related to this occurrence.
    Methods: We performed a retrospective review of 131 patients with craniopharyngioma who underwent surgery from April 2009 to September 2021. The inclusion criteria were initial surgery, endoscopic transsphenoidal surgery, preoperative normal pituitary function or pituitary dysfunction in one axis, and morphological preservation of the pituitary stalk. The branches of the pSHA consist mainly of the chiasmatic branches (Cb), infundibular branches (Ib), and descending branches (Db). We analyzed the association between postoperative pituitary function and preservation of these branches.
    Results: Twenty patients met the criteria. Preoperative anterior pituitary function was normal in 18 patients, and there was isolated growth hormone deficiency in two patients. No patient had preoperative diabetes insipidus (DI). Anterior pituitary function was unchanged postoperatively in eight patients. Of these eight patients, bilateral preservation of pSHA Ib was confirmed in seven patients. Bilateral preservation of pSHA Ib was the only factor associated with preserved anterior pituitary function (p < 0.01). Fifteen patients were free of permanent DI, and the preservation of any given pSHA branch produced no significant difference in the postoperative occurrence of permanent DI.
    Conclusions: Our study shows that bilateral preservation of pSHA Ib provides favorable postoperative anterior pituitary function in craniopharyngioma surgery; however, such preservation may have little effect on the postoperative occurrence of DI.
    MeSH term(s) Humans ; Craniopharyngioma/surgery ; Pituitary Neoplasms/surgery ; Pituitary Gland/surgery ; Diabetes Insipidus/complications ; Postoperative Complications ; Arteries ; Retrospective Studies
    Language English
    Publishing date 2022-11-10
    Publishing country Austria
    Document type Journal Article
    ZDB-ID 80010-7
    ISSN 0942-0940 ; 0001-6268
    ISSN (online) 0942-0940
    ISSN 0001-6268
    DOI 10.1007/s00701-022-05415-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Efficacy and safety of the endoscopic "wet-field" technique for removal of supratentorial cavernous malformations.

    Takeuchi, Kazuhito / Nagata, Yuichi / Tanahashi, Kuniaki / Araki, Yoshio / Mizuno, Akihiro / Sasaki, Hiroo / Harada, Hideyuki / Ito, Keishi / Saito, Ryuta

    Acta neurochirurgica

    2022  Volume 164, Issue 10, Page(s) 2587–2594

    Abstract: Objective: Cerebral cavernous malformations (CMs) presenting with focal neurological symptoms or mass effects require surgical removal. In recent years, cylindrical retractors have been widely utilized for the removal of deep-seated lesions during both ... ...

    Abstract Objective: Cerebral cavernous malformations (CMs) presenting with focal neurological symptoms or mass effects require surgical removal. In recent years, cylindrical retractors have been widely utilized for the removal of deep-seated lesions during both microscopic and endoscopic surgery. In the present study, we evaluated the efficacy and safety of endoscopic transcylinder removal of CMs using a novel wet-field technique.
    Methods: We included 13 patients with supratentorial CMs who had undergone endoscopic transcylinder surgery between April 2013 and March 2022. One patient experienced recurrence of the CM and underwent a second endoscopic transcylinder surgery. Therefore, we retrospectively evaluated 14 procedures. The surgical field was continuously irrigated with artificial cerebrospinal fluid to maintain expansion and visualization of the tumor bed. We termed this method as the "wet-field technique." Patient characteristics, symptoms, and pre- and postoperative magnetic resonance imaging results were obtained from medical records.
    Results: The average maximum CM diameter was 35.3 mm (range: 10-65 mm). Cylinder diameters were 6 mm in eight procedures, 10 mm in four procedures, and 17 mm in one procedure. Wet-field technique was applied in all cases. The endoscope provided a bright field of view even under water. Continuous water irrigation made it easier to observe the entire tumor bed which naturally expanded by water pressure. Gross total resection was achieved in 13 procedures, while subtotal resection was achieved in one procedure. No surgical complications were observed.
    Conclusions: The endoscopic transcylinder removal using wet-field technique is safe and effective for the removal of symptomatic intracranial supratentorial CMs.
    MeSH term(s) Hemangioma, Cavernous, Central Nervous System ; Humans ; Neurosurgical Procedures/methods ; Retrospective Studies ; Treatment Outcome ; Water
    Chemical Substances Water (059QF0KO0R)
    Language English
    Publishing date 2022-06-22
    Publishing country Austria
    Document type Journal Article
    ZDB-ID 80010-7
    ISSN 0942-0940 ; 0001-6268
    ISSN (online) 0942-0940
    ISSN 0001-6268
    DOI 10.1007/s00701-022-05273-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Safety and efficacy of the endoscopic transsphenoidal transclival approach performed using direct cortical stimulation for pontine cavernous malformations.

    Takeuchi, Kazuhito / Nagata, Yuichi / Sasagawa, Yasuo / Ito, Eiji / Yamamoto, Taiki / Mizuno, Akihiro / Sasaki, Hiroo / Kondo, Tatsuma / Araki, Yoshio / Nakada, Mitsutoshi / Saito, Ryuta

    Journal of neurosurgery

    2023  Volume 140, Issue 2, Page(s) 469–477

    Abstract: Objective: Surgical treatment of brainstem cavernous malformations (CMs) is challenging. Surgery using the endoscopic transsphenoidal transclival approach (eTSTCA) is reported as a useful alternative for ventral brainstem CMs. However, CMs located in ... ...

    Abstract Objective: Surgical treatment of brainstem cavernous malformations (CMs) is challenging. Surgery using the endoscopic transsphenoidal transclival approach (eTSTCA) is reported as a useful alternative for ventral brainstem CMs. However, CMs located in the ventral midline of the brainstem are rare, and only a small number of case reports on these CMs treated with the eTSTCA exist. The efficacy and safety of the eTSTCA have not yet been fully examined.
    Methods: A retrospective analysis was performed for 5 consecutive patients who underwent surgery via the eTSTCA for treating ventral pontine CMs.
    Results: The average maximum CM diameter was 26.0 mm (18-38 mm). All patients underwent MR-diffusion tensor imaging, which confirmed that the corticospinal tract (CST) deviated posteriorly or laterally to the CM. Direct brainstem cortical stimulation was performed to localize the CST before making the cortical incision. After the excision of the CM, the cavity was filled with artificial CSF to make an aqueous surgical field (wet-field technique) for observing the tumor cavity and confirming complete hemostasis and resection. Total removal was achieved in all patients. The preoperative modified Rankin Scale score was 3 in 3 patients and 4 in 2 patients, whereas it was 1 in 2 patients and 0 in 3 patients 3 months after surgery. Postoperative CSF leakage was observed in 1 patient, and transient abducens nerve palsy was observed in 1 patient. No other intra- or postoperative complications were observed.
    Conclusions: MR-diffusion tensor imaging and direct brainstem cortical stimulation were useful to ascertain the proximity of the CST to the CM. The endoscope provides a clear view even underwater, and it was safe and effective to observe the entire CM cavity and confirm complete hemostasis without additional retraction of the brainstem parenchyma, including the CST. The eTSTCA provides a direct access point to the lesion and may be a safer alternative treatment for patients whose CST deviates laterally or posteriorly to the CM.
    MeSH term(s) Humans ; Diffusion Tensor Imaging/methods ; Retrospective Studies ; Pons/surgery ; Endoscopy ; Brain Stem/diagnostic imaging ; Brain Stem/surgery ; Brain Stem/pathology ; Postoperative Complications/pathology
    Language English
    Publishing date 2023-08-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3089-2
    ISSN 1933-0693 ; 0022-3085
    ISSN (online) 1933-0693
    ISSN 0022-3085
    DOI 10.3171/2023.6.JNS23324
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Second-best efficiency of allocation rules

    Anno, Hidekazu / Sasaki, Hiroo

    Economic theory Vol. 54, No. 3 , p. 693-716

    strategy-proofness and single-peaked preferences with multiple commodities

    2013  Volume 54, Issue 3, Page(s) 693–716

    Author's details Hidekazu Anno; Hiroo Sasaki
    Keywords Strategy-proofness ; Single-peaked preference ; Second-best efficiency ; Generalized uniform rule
    Language English
    Size graph. Darst.
    Publisher Springer
    Publishing place Berlin ; Heidelberg
    Document type Article
    ZDB-ID 1059110-2 ; 1398355-6
    ISSN 1432-0479 ; 0938-2259
    ISSN (online) 1432-0479
    ISSN 0938-2259
    Database ECONomics Information System

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  8. Article ; Online: Generation of hypothalamic neural stem cell-like cells in vitro from human pluripotent stem cells.

    Miwata, Tsutomu / Suga, Hidetaka / Kawaguchi, Yohei / Sakakibara, Mayu / Kano, Mayuko / Taga, Shiori / Soen, Mika / Ozaki, Hajime / Asano, Tomoyoshi / Sasaki, Hiroo / Miyata, Takashi / Yasuda, Yoshinori / Kobayashi, Tomoko / Sugiyama, Mariko / Onoue, Takeshi / Takagi, Hiroshi / Hagiwara, Daisuke / Iwama, Shintaro / Arima, Hiroshi

    Stem cell reports

    2023  Volume 18, Issue 4, Page(s) 869–883

    Abstract: When damaged, restoring the function of the hypothalamus is currently impossible. It is unclear whether neural stem cells exist in the hypothalamus. Studies have reported that adult rodent tanycytes around the third ventricle function as hypothalamic ... ...

    Abstract When damaged, restoring the function of the hypothalamus is currently impossible. It is unclear whether neural stem cells exist in the hypothalamus. Studies have reported that adult rodent tanycytes around the third ventricle function as hypothalamic neural stem cell-like cells. However, it is currently impossible to collect periventricular cells from humans. We attempted to generate hypothalamic neural stem cell-like cells from human embryonic stem cells (ESCs). We focused on retina and anterior neural fold homeobox (RAX) because its expression is gradually restricted to tanycytes during the late embryonic stage. We differentiated RAX::VENUS knockin human ESCs (hESCs) into hypothalamic organoids and sorted RAX
    MeSH term(s) Mice ; Animals ; Humans ; Neural Stem Cells ; Cell Differentiation/physiology ; Pluripotent Stem Cells ; Neurogenesis/physiology ; Hypothalamus/metabolism
    Language English
    Publishing date 2023-03-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2720528-9
    ISSN 2213-6711 ; 2213-6711
    ISSN (online) 2213-6711
    ISSN 2213-6711
    DOI 10.1016/j.stemcr.2023.02.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Subcutaneous transplantation of human embryonic stem cells-derived pituitary organoids.

    Sasaki, Hiroo / Suga, Hidetaka / Takeuchi, Kazuhito / Nagata, Yuichi / Harada, Hideyuki / Kondo, Tatsuma / Ito, Eiji / Maeda, Sachi / Sakakibara, Mayu / Soen, Mika / Miwata, Tsutomu / Asano, Tomoyoshi / Ozaki, Hajime / Taga, Shiori / Kuwahara, Atsushi / Nakano, Tokushige / Arima, Hiroshi / Saito, Ryuta

    Frontiers in endocrinology

    2023  Volume 14, Page(s) 1130465

    Abstract: Introduction: The pituitary gland, regulating various hormones, is central in the endocrine system. As spontaneous recovery from hypopituitarism is rare, and exogenous-hormone substitution is clumsy, pituitary replacement : Methods: hESC-derived POs ... ...

    Abstract Introduction: The pituitary gland, regulating various hormones, is central in the endocrine system. As spontaneous recovery from hypopituitarism is rare, and exogenous-hormone substitution is clumsy, pituitary replacement
    Methods: hESC-derived POs were transplanted into inguinal subcutaneous white adipose tissue (ISWAT) and beneath dorsal skin, a relatively avascular region (AR), of hypophysectomized severe combined immunodeficient (SCID) mice. Pituitary function was evaluated thereafter for ¾ 6mo, assaying basal plasma ACTH and ACTH response to corticotropin-releasing hormone (CRH) stimulation. Histopathologic examination of organoids 150d after transplantation assessed engraftment. Some mice received an inhibitor of vascular endothelial growth factor (VEGF) to permit assessment of how angiogenesis contributed to subcutaneous engraftment.
    Results: During follow-up, both basal and CRH-stimulated plasma ACTH levels were significantly higher in the ISWAT group (
    Conclusions: Subcutaneous transplantation of hESC-derived POs into hypopituitary SCID mice efficaciously renders recipients ACTH-sufficient.
    MeSH term(s) Humans ; Mice ; Animals ; Human Embryonic Stem Cells/metabolism ; Vascular Endothelial Growth Factor A/metabolism ; Adrenocorticotropic Hormone/metabolism ; Corticotropin-Releasing Hormone/metabolism ; Mice, SCID ; Pituitary Gland/metabolism ; Pituitary Diseases/metabolism ; Hypopituitarism/metabolism
    Chemical Substances Vascular Endothelial Growth Factor A ; Adrenocorticotropic Hormone (9002-60-2) ; Corticotropin-Releasing Hormone (9015-71-8)
    Language English
    Publishing date 2023-03-02
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2592084-4
    ISSN 1664-2392
    ISSN 1664-2392
    DOI 10.3389/fendo.2023.1130465
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Direct intracranial invasion of eccrine spiradenocarcinoma of the scalp: a case report and literature review.

    Kibe, Yuji / Tanahashi, Kuniaki / Ohtakara, Kazuhiro / Okumura, Yuka / Ohka, Fumiharu / Takeuchi, Kazuhito / Nagata, Yuichi / Motomura, Kazuya / Akahori, Sho / Mizuno, Akihiro / Sasaki, Hiroo / Shimizu, Hiroyuki / Yamaguchi, Junya / Nishikawa, Tomohide / Yokota, Kenji / Saito, Ryuta

    BMC neurology

    2022  Volume 22, Issue 1, Page(s) 223

    Abstract: Background: Eccrine spiradenocarcinoma (SC), also known as malignant eccrine spiradenoma, is a rare malignant cutaneous adnexal neoplasm arising from long-standing benign eccrine spiradenoma. Malignant skin tumors rarely show direct intracranial ... ...

    Abstract Background: Eccrine spiradenocarcinoma (SC), also known as malignant eccrine spiradenoma, is a rare malignant cutaneous adnexal neoplasm arising from long-standing benign eccrine spiradenoma. Malignant skin tumors rarely show direct intracranial invasion. However, once the intracranial structure is infiltrated, curative excision with sufficient margins can become extremely difficult, particularly when the venous sinuses are involved. No effective adjuvant therapies have yet been established. Here, we report an extremely rare case of scalp eccrine SC with direct intracranial invasion, which does not appear to have been reported previously.
    Case presentation: An 81-year-old woman presented with a large swelling on the parietal scalp 12 years after resection of spiradenoma from the same site. The tumor showed intracranial invasion with involvement of the superior sagittal sinus and repeated recurrences after four surgeries with preservation of the sinus. The histopathological diagnosis was eccrine SC. Adjuvant high-precision external beam radiotherapy (EBRT) proved effective after the third surgery, achieving remission of the residual tumor. The patient died 7 years after the first surgery for SC.
    Conclusions: Scalp SC with direct intracranial invasion is extremely rare. Radical resection with tumor-free margins is the mainstay of treatment, but the involvement of venous sinuses makes this unfeasible. High-precision EBRT in combination with maximal resection preserving the venous sinuses could be a treatment option for local tumor control.
    MeSH term(s) Acrospiroma/pathology ; Acrospiroma/surgery ; Aged, 80 and over ; Female ; Humans ; Scalp/pathology ; Scalp/surgery ; Sweat Gland Neoplasms/pathology ; Sweat Gland Neoplasms/surgery
    Language English
    Publishing date 2022-06-18
    Publishing country England
    Document type Case Reports ; Journal Article ; Review
    ZDB-ID 2041347-6
    ISSN 1471-2377 ; 1471-2377
    ISSN (online) 1471-2377
    ISSN 1471-2377
    DOI 10.1186/s12883-022-02749-4
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