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Article: Artificial Intelligence: A Next-Level Approach in Confronting the COVID-19 Pandemic.

Mahalakshmi, V / Balobaid, Awatef / Kanisha, B / Sasirekha, R / Ramkumar Raja, M

2023 Volume 11, Issue 6

Abstract: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which caused coronavirus diseases (COVID-19) in late 2019 in China created a devastating economical loss and loss of human lives. To date, 11 variants have been identified with minimum to ... ...

Abstract	The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which caused coronavirus diseases (COVID-19) in late 2019 in China created a devastating economical loss and loss of human lives. To date, 11 variants have been identified with minimum to maximum severity of infection and surges in cases. Bacterial co-infection/secondary infection is identified during viral respiratory infection, which is a vital reason for morbidity and mortality. The occurrence of secondary infections is an additional burden to the healthcare system; therefore, the quick diagnosis of both COVID-19 and secondary infections will reduce work pressure on healthcare workers. Therefore, well-established support from Artificial Intelligence (AI) could reduce the stress in healthcare and even help in creating novel products to defend against the coronavirus. AI is one of the rapidly growing fields with numerous applications for the healthcare sector. The present review aims to access the recent literature on the role of AI and how its subfamily machine learning (ML) and deep learning (DL) are used to curb the pandemic's effects. We discuss the role of AI in COVID-19 infections, the detection of secondary infections, technology-assisted protection from COVID-19, global laws and regulations on AI, and the impact of the pandemic on public life.
Language	English
Publishing date	2023-03-14
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2721009-1
ISSN	2227-9032
ISSN	2227-9032
DOI	10.3390/healthcare11060854
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Article ; Online: Smart Poultry House Monitoring System Using IoT

Sasirekha R. / R Kaviya / G Saranya / Mohamed Aezeden / Iroda Utaeva

E3S Web of Conferences, Vol 399, p

2023 Volume 04055

Abstract: In modern India, the poultry industry is one of the largest and fastest growing segments of the agrarian economy. Due to standardized farming management and excellent manufacturing practices, chicken output has been steadily increasing in recent years. ... ...

Abstract	In modern India, the poultry industry is one of the largest and fastest growing segments of the agrarian economy. Due to standardized farming management and excellent manufacturing practices, chicken output has been steadily increasing in recent years. Automation is crucial in the modern world, and the Internet of Things (IoT) concept is also evolving rapidly. It is possible to automate manual processes using acertain technique. The project's objective is to use IoT technologies to automate management-related chores on a chicken farm. Environmental factors like temperature and humidity are just a few that have an impact on chicken wellness. By keeping an eye on the chicks according to their cycle, the proprietor is informed. The weight of the chicken is taken into consideration for high - quality manufacturing. If all these factors remain consistent, chicken production and quality increase.
Keywords	iot ; alarm ; sensor ; coop ; chicken detection ; temperature ; light ; water level ; humidity ; node mcu ; thingspeak ; mymqtt ; Environmental sciences ; GE1-350
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	EDP Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: In silico analysis of diversity, specificity and molecular evolution of Stenotrophomonas phages

Sasirekha, Revathy / Sharma, Osheen / Sugumar, Shobana

Environmental microbiology reports. 2022 June, v. 14, no. 3

2022

Abstract: In this study, we have focused on the lytic phage proteins encoded by the Stenotrophomonas phages. A total of 60 lytic proteins were identified to be encoded by 19 different phages. Those proteins were characterized under eight classes: amidases, ... ...

Abstract	In this study, we have focused on the lytic phage proteins encoded by the Stenotrophomonas phages. A total of 60 lytic proteins were identified to be encoded by 19 different phages. Those proteins were characterized under eight classes: amidases, muramidases, pectate lyase, peptidases, holins and spanins. The phages encoding these proteins come under the family of Ackermannviridae, Autographiviridae, Myoviridae, Podoviridae and Siphoviridae. All the phages encoding those proteins were found to infect Stenotrophomonas maltophilia. Among the phages, about 50% were found to undergo a lytic lifecycle. The isolated proteins were clustered according to the similarity in the amino acid sequence. These clusters were used to make their phylogenetic tree. The co‐occurrence of the amidase, pectate lyase and lipase genes in the phage genome was found using a correlation analysis.
Keywords	Ackermannviridae ; Autographiviridae ; Myoviridae ; Podoviridae ; Siphoviridae ; Stenotrophomonas maltophilia ; amidase ; amino acid sequences ; bacteriophages ; carboxylic ester hydrolases ; computer simulation ; pectate lyase ; peptidases ; phylogeny
Language	English
Dates of publication	2022-06
Size	p. 422-430.
Publishing place	John Wiley & Sons, Inc.
Document type	Article
Note	JOURNAL ARTICLE
ISSN	1758-2229
DOI	10.1111/1758-2229.13025
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: In silico analysis of diversity, specificity and molecular evolution of Stenotrophomonas phages.

Sasirekha, Revathy / Sharma, Osheen / Sugumar, Shobana

Environmental microbiology reports

2021 Volume 14, Issue 3, Page(s) 422–430

Abstract	In this study, we have focused on the lytic phage proteins encoded by the Stenotrophomonas phages. A total of 60 lytic proteins were identified to be encoded by 19 different phages. Those proteins were characterized under eight classes: amidases, muramidases, pectate lyase, peptidases, holins and spanins. The phages encoding these proteins come under the family of Ackermannviridae, Autographiviridae, Myoviridae, Podoviridae and Siphoviridae. All the phages encoding those proteins were found to infect Stenotrophomonas maltophilia. Among the phages, about 50% were found to undergo a lytic lifecycle. The isolated proteins were clustered according to the similarity in the amino acid sequence. These clusters were used to make their phylogenetic tree. The co-occurrence of the amidase, pectate lyase and lipase genes in the phage genome was found using a correlation analysis.
MeSH term(s)	Bacteriophages ; Evolution, Molecular ; Genome, Viral ; Phylogeny ; Stenotrophomonas/genetics
Language	English
Publishing date	2021-11-18
Publishing country	United States
Document type	Journal Article
ISSN	1758-2229
ISSN (online)	1758-2229
DOI	10.1111/1758-2229.13025
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Article ; Online: Ruptured renal cell carcinoma in pregnancy

Prameela RC / Sasirekha R

International Journal of Reproduction, Contraception, Obstetrics and Gynecology, Vol 5, Iss 5, Pp 1677-

a rare case presentation

2016 Volume 1679

Abstract: Malignancy in pregnancy is rare. Carcinomas in pregnancy are mostly kidney cell mass. Renal cell carcinoma (RCC) is the commonest malignancy in pregnancy. Because of softness and increased vascularity, rupture of renal cell carcinoma is not uncommon. ... ...

Abstract	Malignancy in pregnancy is rare. Carcinomas in pregnancy are mostly kidney cell mass. Renal cell carcinoma (RCC) is the commonest malignancy in pregnancy. Because of softness and increased vascularity, rupture of renal cell carcinoma is not uncommon. Here we are presenting a rare case of renal cell carcinoma in pregnancy with spontaneous rupture resulting in massive hemoperitoneum and serious outcome because of late presentation renal cell carcinoma seldom ruptures. A 26 year old woman G2P1L1 with term pregnancy was referred to hospital 80kms away from periphery with non-progression of labour. There was antenatal record suggesting hypertensive disorder of pregnancy in second trimester. On examination, patient was in hypovolemic shock with profuse distension of abdomen. Diagnosis of abruption grade 3 or rupture uterus was made and immediate laparotomy was done. On opening the abdomen, there was hemoperitoneum but uterus was intact. Emergency LSCS done extracted a stillborn baby. There were no retro placental clots also. There was lot of necrotic tissue in the abdomen and there was a tumour arising from lower pole of left kidney which had invaded the renal vessels and had ruptured. Peripartum hysterectomy and left nephrectomy was done. Women did not respond to treatment and died. The objective of presenting this case is the dilemmas faced by the obstetrician in case of shock in 2nd stage of labour. Simple diagnostic tool like renal ultrasound will help to detect at an early stage which could improve the outcome. All cases of hypertensive disorders of pregnancy should be investigated for secondary causes of hypertension. Abdominal USG must be done for all cases of hypertensive disorders of pregnancy in 2nd trimester. Prompt diagnosis and early treatment is the key in management of such condition in pregnancy. [Int J Reprod Contracept Obstet Gynecol 2016; 5(5.000): 1677-1679]
Keywords	Renal cell carcinoma ; Pregnancy ; Rupture ; Gynecology and obstetrics ; RG1-991 ; Medicine ; R
Subject code	616
Language	English
Publishing date	2016-05-01T00:00:00Z
Publisher	Medip Academy
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Successful management of pregnancy in a non-communicating rudimentary horn of a unicornuate uterus

Sasirekha R. / Reddi P. Rani

International Journal of Reproduction, Contraception, Obstetrics and Gynecology, Vol 4, Iss 6, Pp 2059-

2015 Volume 2061

Abstract: Pregnancy in a rudimentary horn pregnancy is a rare clinical condition. The usual consequence is rupture culminating in hypovolemic shock. We had a different scenario of unruptured rudimentary horn pregnancy which was detected following failed attempt of ...

Abstract	Pregnancy in a rudimentary horn pregnancy is a rare clinical condition. The usual consequence is rupture culminating in hypovolemic shock. We had a different scenario of unruptured rudimentary horn pregnancy which was detected following failed attempt of MTP. She underwent laparotomy and excision of rudimentary horn. Post-operative period was uneventful. We present this case not only because of rarity but also high index of suspicion is required to diagnose at an early stage before a devastating course. [Int J Reprod Contracept Obstet Gynecol 2015; 4(6.000): 2059-2061]
Keywords	Pregnancy ; Rudimentary horn ; Un ruptured ; Gynecology and obstetrics ; RG1-991 ; Medicine ; R
Language	English
Publishing date	2015-12-01T00:00:00Z
Publisher	Medip Academy
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Surface engineered Amphora subtropica frustules using chitosan as a drug delivery platform for anticancer therapy.

Sasirekha, Rajendran / Sheena, Thankaraj Salammal / Sathiya Deepika, Murugesan / Santhanam, Perumal / Townley, Helen E / Jeganathan, K / Dinesh Kumar, Sundarrajan / Premkumar, Kumpati

Materials science & engineering. C, Materials for biological applications

2018 Volume 94, Page(s) 56–64

Abstract: Drug delivery using synthetic mesoporous nanomaterials, including porous silicon, has been extensively used to ameliorate the constraints currently experienced with conventional chemotherapy. Owing to the amazing potential, the silica based nanomaterials ...

Abstract	Drug delivery using synthetic mesoporous nanomaterials, including porous silicon, has been extensively used to ameliorate the constraints currently experienced with conventional chemotherapy. Owing to the amazing potential, the silica based nanomaterials have been used widely. Nevertheless, synthetic nanomaterial involves high cost, lack of scalability, and the use of toxic substances limits its utilization. These issues can be overcome by the use of nature generated nanoscale materials, such as diatoms would serve as a boon for pharmaceutical industries. In this study we investigate the use of a mesoporous, biodegradable nanomaterial obtained from the natural silica found in the diatom species Amphora subtropica (AMPS) for drug delivery applications. AMPS cultures cleaned and chemically treated to obtain Amphora frustules (exoskeleton) (AF), followed by surface functionalization with chitosan (Chi). Results of our experiments demonstrate high drug loading, strong luminescence, biodegradable and biocompatible nature of the doxorubicin tethered diatom. Further, toxicity studies employing immortalized lung cancer cell line (A549) indicates sustained drug delivery and less toxic compared to the free doxorubicin (DOX), suggesting AF could be an excellent substitute for synthetic nanomaterials used in drug delivery applications.
MeSH term(s)	A549 Cells ; Antineoplastic Agents/pharmacology ; Cell Death/drug effects ; Chitosan/chemistry ; Diatoms/chemistry ; Drug Delivery Systems ; Humans ; Materials Testing ; Microalgae/isolation & purification ; Phylogeny ; Silicon Dioxide/chemistry ; Spectroscopy, Fourier Transform Infrared ; Static Electricity ; Surface Properties
Chemical Substances	Antineoplastic Agents ; Silicon Dioxide (7631-86-9) ; Chitosan (9012-76-4)
Language	English
Publishing date	2018-09-06
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2012160-X
ISSN	1873-0191 ; 0928-4931
ISSN (online)	1873-0191
ISSN	0928-4931
DOI	10.1016/j.msec.2018.09.009
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Article ; Online: A novel rutin-fucoidan complex based phytotherapy for cervical cancer through achieving enhanced bioavailability and cancer cell apoptosis.

Deepika, Murugesan Sathiya / Thangam, Ramar / Sheena, Thankaraj Salammal / Sasirekha, Rajendran / Sivasubramanian, Srinivasan / Babu, Manikandan Dinesh / Jeganathan, Kulandaivel / Thirumurugan, Ramasamy

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

2018 Volume 109, Page(s) 1181–1195

Abstract: Recent studies on flavonoids forming complexes with macromolecules attract researchers due to their enhanced bioavailability as well as chemo-preventive efficacy. In this study, a flavonoid rutin (Ru) is non-covalently complexed with fucoidan (Fu) using ... ...

Abstract	Recent studies on flavonoids forming complexes with macromolecules attract researchers due to their enhanced bioavailability as well as chemo-preventive efficacy. In this study, a flavonoid rutin (Ru) is non-covalently complexed with fucoidan (Fu) using the functional groups to obtain a therapeutic polymeric complex overcoming the limitations of bioavailability of rutin. The prepared novel rutin-fucoidan (Ru-Fu) complex is characterized for spectroscopic features, particle size and distribution analysis by DLS. It is shown that the complex displayed the nanostructural features that are different from that of the usual rutin-fucoidan mixture. The studies on drug release profiles at different pH (5.5, 6.8 and 7.4) show that the sustained release of compounds from complex occurs preferentially at the desired endosomal pH (5.5). Further, the chemopreventive potential of Ru-Fu complex is investigated against HeLa cells by cellular apoptotic assays and flow cytometric analysis. It showed that the complex is able to disrupt cell cycle regulation and has the ability to induce cellular apoptosis via nuclear fragmentation, ROS generation and mitochondrial potential loss. In vitro cell viability assay with Ru-Fu complex shows that the complex is biocompatible on normal cells. The hemolysis assay also reveals that the complex does not release hemoglobin from human red blood cells (RBCs). Thus, the study is envisaged to open up interests for developing such formulations against cervical cancer and other cancers.
MeSH term(s)	Apoptosis/drug effects ; Biological Availability ; Cell Cycle Checkpoints/drug effects ; Cell Line, Tumor ; Cell Survival/drug effects ; Female ; Flavonoids/pharmacology ; HeLa Cells ; Humans ; Mitochondria/drug effects ; Phytotherapy/methods ; Polysaccharides/pharmacology ; Rutin/pharmacology ; Uterine Cervical Neoplasms/drug therapy
Chemical Substances	Flavonoids ; Polysaccharides ; Rutin (5G06TVY3R7) ; fucoidan (9072-19-9)
Language	English
Publishing date	2018-11-06
Publishing country	France
Document type	Journal Article
ZDB-ID	392415-4
ISSN	1950-6007 ; 0753-3322 ; 0300-0893
ISSN (online)	1950-6007
ISSN	0753-3322 ; 0300-0893
DOI	10.1016/j.biopha.2018.10.178
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Article ; Online: Antibacterial synergy between rutin and florfenicol enhances therapeutic spectrum against drug resistant Aeromonas hydrophila.

Deepika, Murugesan Sathiya / Thangam, Ramar / Vijayakumar, Tharumasivam Siva / Sasirekha, Rajendran / Vimala, R T V / Sivasubramanian, Srinivasan / Arun, Sridhar / Babu, Manikandan Dinesh / Thirumurugan, Ramasamy

Microbial pathogenesis

2019 Volume 135, Page(s) 103612

Abstract: Emergence of antibiotic resistant bacteria has necessitated the drive to explore competent antimicrobial agents or to develop novel formulations to treat infections including Aeromonas hydrophila. The present study investigates the synergistic ... ...

Abstract	Emergence of antibiotic resistant bacteria has necessitated the drive to explore competent antimicrobial agents or to develop novel formulations to treat infections including Aeromonas hydrophila. The present study investigates the synergistic antibacterial effects of citrus flavonoid rutin and florfenicol (FF) against A. hydrophila in vitro and in vivo. Rutin is extracted and purified from Citrus sinensis peel through preparative HPLC and characterized through TLC, GC-MS and
MeSH term(s)	Aeromonas hydrophila/drug effects ; Aeromonas hydrophila/growth & development ; Animals ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Biofilms/drug effects ; Citrus sinensis/chemistry ; DNA Damage/drug effects ; Disease Models, Animal ; Drug Combinations ; Drug Synergism ; Fish Diseases/drug therapy ; Fish Diseases/immunology ; Fish Diseases/microbiology ; Fish Diseases/pathology ; Fisheries ; Gram-Negative Bacterial Infections/drug therapy ; Gram-Negative Bacterial Infections/veterinary ; Immunity/drug effects ; Immunomodulation ; India ; Microbial Sensitivity Tests ; Oxidative Stress/drug effects ; Plant Extracts/pharmacology ; Rutin/immunology ; Rutin/pharmacology ; Rutin/therapeutic use ; Thiamphenicol/analogs & derivatives ; Thiamphenicol/immunology ; Thiamphenicol/pharmacology ; Thiamphenicol/therapeutic use ; Tilapia/microbiology ; Virulence/drug effects
Chemical Substances	Anti-Bacterial Agents ; Drug Combinations ; Plant Extracts ; Rutin (5G06TVY3R7) ; florfenicol (9J97307Y1H) ; Thiamphenicol (FLQ7571NPM)
Language	English
Publishing date	2019-06-25
Publishing country	England
Document type	Journal Article
ZDB-ID	632772-2
ISSN	1096-1208 ; 0882-4010
ISSN (online)	1096-1208
ISSN	0882-4010
DOI	10.1016/j.micpath.2019.103612
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Article ; Online: Frequency and Antimicrobial Sensitivity Pattern Of Extended Spectrum β-Lactamases Producing E. Coli And Klebsiella Pneumoniae Isolated In A Tertiary Care Hospital

B. Sasirekha, R. Manasa, P. Ramya and R. Sneha

Al Ameen Journal of Medical Sciences , Vol 03, Iss 04, Pp 265-

2010 Volume 271

Abstract: Infections due to extended spectrum β-lactamases (ESBL) producing Escherichia coli and Klebsiella pneumoniae have become an important clinical problem. Local knowledge of antimicrobial susceptibilities of these organisms is important for implementation ... ...

Abstract	Infections due to extended spectrum β-lactamases (ESBL) producing Escherichia coli and Klebsiella pneumoniae have become an important clinical problem. Local knowledge of antimicrobial susceptibilities of these organisms is important for implementation of effective hospital anti-infective policies. The present study was undertaken to determine the prevalence of ESBLs along with their antimicrobial sensitivity pattern in Escherichia coli and klebsiella pneumoniae isolates at a tertiary care hospital in Bangalore. A total of 243 clinical isolates comprising E. coli (n=157) and K. pneumoniae (n=86) were recovered from various clinical samples over a period of six months from June to November 2008. Antibiogram profile of these isolates was determined by Kirby-Bauer’s disc diffusion method. All the isolates were screened for ESBL production as recommended by the Clinical Laboratory Standard Institute (CLSI). The present findings revealed a high occurrence of multidrug resistance ESBL producing E. coli (61.1%) and K. pneumoniae (40.6%). Imipenem was highly active against ESBL producing isolates. Results indicate that routine ESBL detection should be made imperative and empirical use of third generation cephaolsporins must be discouraged.
Keywords	Extended spectrum β-lactamases ; Escherichia coli ; Klebsiella pneumoniae ; third generation cephalosporin ; Medicine (General) ; R5-920 ; Medicine ; R ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
Language	English
Publishing date	2010-10-01T00:00:00Z
Publisher	Dr.B.S.Patil
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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