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Article: A Study on Knowledge, Attitude, and Practice Related to Sexually Transmitted Infections Among Students of Nonmedical Background in Manipal, India.

Vasudev, Meghana B / Ballambat, Sathish P / Shetty, Varsha M

2023 Volume 15, Issue 1, Page(s) 39–44

Abstract: Background: Sexually transmitted infections (STIs) are one of the major public health problems in India with adolescents being a vulnerable section of the affected population. According to the World Health Organization (WHO), one in twenty adolescents ... ...

Abstract	Background: Sexually transmitted infections (STIs) are one of the major public health problems in India with adolescents being a vulnerable section of the affected population. According to the World Health Organization (WHO), one in twenty adolescents contracts an STI every year, excluding the human immunodeficiency virus (HIV) and other viral infections. It is important to assess the knowledge, attitude, and practices related to STIs among adolescents and young adults of non-medical backgrounds to educate them properly about protective and preventive measures and to allay misconceptions, if any. Materials and methods: This is a cross-sectional study involving 507 engineering students who were asked to fill out the predesigned anonymous questionnaire form. Results were statistically analyzed and tabulated. Results: Of the 507 students, 375 were males and 132 were females. The mean age of the patients was 20.5 years. HIV was the most commonly identified STI (94.2%), followed by herpes genitalis (76.5%). Around 97.4% and 85.7% identified the sexual route and blood transfusion as the modes of transmission of STIs, respectively. A total of 90.9% said that the Internet was their primary source of information regarding STIs. Infertility and cancer were identified as complications by 59.5% and 25% of them, respectively. Only 23.4% were aware of the National STI Control Program. The results with respect to attitudes and practices related to STIs were variable. Conclusion: Our study reflects that with change in time, the attitude of young adults toward sexuality is becoming very casual, but knowledge remains low. A lot of educational activities should be undertaken to educate our youth about various aspects of STIs and thus reduce the burden of the disease in the community.
Language	English
Publishing date	2023-12-22
Publishing country	India
Document type	Journal Article
ZDB-ID	2585814-2
ISSN	2249-5673 ; 2229-5178
ISSN (online)	2249-5673
ISSN	2229-5178
DOI	10.4103/idoj.idoj_687_22
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Article ; Online: Elevated CO

Vanaja, M / Sarkar, B / Sathish, P / Jyothi Lakshmi, N / Yadav, S K / Mohan, Ch / Sushma, A / Yashavanth, B S / Srinivasa Rao, M / Prabhakar, M / Singh, V K

Scientific reports

2024 Volume 14, Issue 1, Page(s) 2928

Abstract: The rising temperatures and levels of carbon dioxide in the atmosphere are anticipated to have a significant impact on the productivity of agricultural crops. Although, the individual effects of elevated ... ...

Abstract	The rising temperatures and levels of carbon dioxide in the atmosphere are anticipated to have a significant impact on the productivity of agricultural crops. Although, the individual effects of elevated CO
MeSH term(s)	Carbon Dioxide/metabolism ; Temperature ; Zea mays ; Photosynthesis/physiology ; Edible Grain/metabolism ; Crops, Agricultural/metabolism
Chemical Substances	Carbon Dioxide (142M471B3J)
Language	English
Publishing date	2024-02-05
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-53343-2
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Article ; Online: Impact of effluent parameters and vancomycin concentration on vancomycin resistant Escherichia coli and its host specific bacteriophage lytic activity in hospital effluent.

Nithiya, P / Alagarsamy, G / Sathish, P B / Rajarathnam, D / Li, Xu / Jeyaraj, Sankarganesh / Satheesh, Manjima / Selvakumar, R

Environmental research

2024 Volume 247, Page(s) 118334

Abstract: Vancomycin resistance in bacteria has been classified under high priority category by World Health Organization (WHO) and its presence in hospital effluent is reported to be increasing owing to excess antibiotics use. Among various strategies, ... ...

Abstract	Vancomycin resistance in bacteria has been classified under high priority category by World Health Organization (WHO) and its presence in hospital effluent is reported to be increasing owing to excess antibiotics use. Among various strategies, bacteriophage has been recently considered as a promising biological agent for combating such antimicrobial resistant bacteria (ARB). However, the influence of effluent's properties on phage-ARB interaction in actual hospital effluent is not completely understood. The present works intends to study this influence of hospital effluent and its parameters on the interaction between vancomycin resistant E. coli (VRE) and its host specific bacteriophage. The isolated VRE was identified by 16S rRNA sequencing, matrix-assisted laser desorption/ionization-time of flight (MALDI - TOF) and whole genome sequencing. The infectivity of phage onto host bacteria was investigated using electron microscopic techniques, dynamic light scattering (DLS), spectrofluorophotometer and confirmed using double agar overlay method. The monovalency and polyvalency of isolated phage against various bacterial species were determined. The phage morphology was identical to T7 phage belonging to Podoviridae. The phage lysis was maximum at pH 7 (90.2%), 37 °C (91.6%) and vancomycin concentration of 50 μg/mL in both synthetic media (89.13%) and effluent (100%). At a maximum vancomycin concentration of 100 μg/mL, decrease in Ca, K, Mg and P (up to 19.70, 14.18, 28, and 15.82% respectively) concentration in effluent was observed due to phage infectivity when compared to control. The whole genome sequencing was performed and the bioinformatics analysis presented the role of mdfA gene encoding the efflux pump in causing vancomycin resistance in E. coli. It also depicted the presence of multiple genes responsible for mercury, cobalt, zinc and cadmium resistance in VRE. These results clearly indicate that bacteriophage mediated combating of VRE is possible in actual hospital effluent and can be used as one of the treatment methods.
MeSH term(s)	Vancomycin/pharmacology ; Bacteriophages ; Escherichia coli/genetics ; Angiotensin Receptor Antagonists ; RNA, Ribosomal, 16S ; Angiotensin-Converting Enzyme Inhibitors ; Hospitals
Chemical Substances	Vancomycin (6Q205EH1VU) ; Angiotensin Receptor Antagonists ; RNA, Ribosomal, 16S ; Angiotensin-Converting Enzyme Inhibitors
Language	English
Publishing date	2024-02-03
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	205699-9
ISSN	1096-0953 ; 0013-9351
ISSN (online)	1096-0953
ISSN	0013-9351
DOI	10.1016/j.envres.2024.118334
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Zs.A 726: Show issues

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Article ; Online: Mutation profile of neurodegenerative mitochondriopathy - LHON in Southern India.

Gowri, Poigaialwar / Sathish, Ponraj / Mahesh Kumar, Shanmugam / Sundaresan, Periasamy

Gene

2022 Volume 819, Page(s) 146202

Abstract: Background: Leber's Hereditary Optic Neuropathy (LHON) is a rare mitochondriopathy causing retinal ganglion cell degeneration resulting in central vision loss. It is caused by mitochondrial DNA (mtDNA) mutations and thus follows maternal inheritance ... ...

Abstract	Background: Leber's Hereditary Optic Neuropathy (LHON) is a rare mitochondriopathy causing retinal ganglion cell degeneration resulting in central vision loss. It is caused by mitochondrial DNA (mtDNA) mutations and thus follows maternal inheritance pattern. Methods: We analysed the whole mitochondrial genome in 100 South Indian LHON patients by utilizing Sanger and Next Generation Sequencing approaches. Haplogroup analysis was performed using HaploGrep2 to predict the risk group. Methylation changes in the mtDNA D-loop region were investigated by performing methylation-specific polymerase chain reaction (MSP). Results: LHON associated mutations were detected in 55% of the patients of which 42% harboured the primary mutations and 13% harboured potentially pathogenic variants that were previously reported to cause LHON. The candidate mutations identified with confirmed pathogenicity are: m.11778G > A (38%), m.14484 T > C (3%), m.4171C > A (1%) and m.11696G > A (1%). MSP results demonstrated that the D-loop region was unmethylated in all the study subjects including mutation-positive patients, mutation-negative patients, asymptomatic carriers, and controls. Haplogroup-M was prevalent (69%) in the study cohort followed by R (14%), U (9%), N (3%), HV (2%), G (2%), and W (1%). The frequency of the predominant mutation m.11778G > A was found lower (̴ 11%) in haplogroup-U. Conclusions: South Indian LHON cohort shows a unique profile of mtDNA mutations and haplogroup association presumably with no role of D-loop methylation. MT-ND4, MT-ND5, and MT-ND1 serve as the hotspot genes in this cohort. The presence of LHON associated mutations in patients lacking the common primary mutations insists on the necessity of mitochondrial genome sequencing in individuals suspected with LHON.
MeSH term(s)	Adolescent ; Adult ; Asians/genetics ; Child ; Child, Preschool ; DNA, Mitochondrial/metabolism ; Female ; Genes, Mitochondrial ; Genetic Predisposition to Disease ; Genome, Mitochondrial ; Humans ; India ; Male ; Methylation ; Middle Aged ; Mitochondria/genetics ; Mutation ; Neurodegenerative Diseases/genetics ; Optic Atrophy, Hereditary, Leber/genetics ; Pedigree ; Phylogeny ; Young Adult
Chemical Substances	DNA, Mitochondrial
Language	English
Publishing date	2022-01-30
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	391792-7
ISSN	1879-0038 ; 0378-1119
ISSN (online)	1879-0038
ISSN	0378-1119
DOI	10.1016/j.gene.2022.146202
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Zs.A 1357: Show issues

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Article: Impact of water deficit stress on traits influencing the drought tolerance and yield of maize (Zea mays L.) genotypes

Sathish, P. / Vanaja, M. / Jyothi Lakshmi, N. / Sarkar, B. / Vijay Kumar, G. / Vagheera, P. / Mohan, C. H. / Maheswari, M.

Plant Physiology Reports. 2022 Mar., v. 27, no. 1

2022

Abstract: Water deficit stress is one of the major abiotic stresses that severely affects the plant performance and production especially when it occurs during the reproductive phase. That drought research is an ongoing approach as new breeding lines development ... ...

Abstract	Water deficit stress is one of the major abiotic stresses that severely affects the plant performance and production especially when it occurs during the reproductive phase. That drought research is an ongoing approach as new breeding lines development is a continuous process. Therefore, a field experiment was conducted with recently developed 12 maize (Zea mays L.) genotypes under Well Water (WW) and Water Deficit stress (WDS) conditions to quantify the impact of WD stress on phenological, physio-biochemical and yield parameters. The results showed that WD stress significantly altered the Anthesis Silking Interval (ASI) among the genotypes but had no effect on the genotypes DTL-4–1 and Harsha. A significant decrease in net photosynthetic rate, stomatal conductance, transpiration rate and WUE was recorded with WD stress. Accumulation of proline under WD stress was significantly higher with DTL-4–1 and Harsha with a lower malondialdehyde (MDA) content and increased activity of SOD thereby maintained better cell membrane stability (CMS). Biomass and grain yield components were also significantly impacted by the water deficit stress. Among the maize genotypes, DTL-4, DTL-4–1 and Harsha showed better Stress tolerance index (STI). Under WD stress, grain yield showed significant positive association with proline, CMS, kernel number while negative association with MDA and ASI, indicating importance of these traits in imparting WD stress tolerance in maize.
Keywords	Zea mays ; biomass ; cell membranes ; corn ; drought ; drought tolerance ; field experimentation ; flowering ; grain yield ; malondialdehyde ; phenology ; photosynthesis ; proline ; seeds ; stomatal conductance ; stress tolerance ; water stress ; well water
Language	English
Dates of publication	2022-03
Size	p. 109-118.
Publishing place	Springer India
Document type	Article
ZDB-ID	3010000-8
ISSN	2662-2548 ; 2662-253X
ISSN (online)	2662-2548
ISSN	2662-253X
DOI	10.1007/s40502-021-00640-x
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Article ; Online: Recycled mesoporous magnetic composites with high surface area derived from plastic and de-oiled sludge wastes: An empirical comparison on their competitive performance for toxic Cr (VI) removal.

Nirenjan Shenoy, P N / Arjun, N M / Senthil Kumar, P / Sree Hari, A B / Nithya, K / Asha Sathish, P

Chemosphere

2021 Volume 292, Page(s) 133375

Abstract: The current study focused on the comparative removal of hexavalent chromium using two magnetically modified hybrid adsorbent composites. Their precursor chars were prepared from bio sludge and plastic waste derivatives. The coating of magnetite on the ... ...

Abstract	The current study focused on the comparative removal of hexavalent chromium using two magnetically modified hybrid adsorbent composites. Their precursor chars were prepared from bio sludge and plastic waste derivatives. The coating of magnetite on the chars' surface was evident from the SEM micrographs. Infusion of magnetite nanoparticles in the native chars aided in the reduction of the composite particles' sizes, thus, forming high surface area composites. Screening of uptake capacities among various blends of char and magnetite were surveyed. Composites of both kinds with a 1:5 ratio of char: iron salts composition answered well. The pH
Language	English
Publishing date	2021-12-21
Publishing country	England
Document type	Journal Article
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2021.133375
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Article: Impact of Elevated CO2 on Two Successive Generations of CO2 Responsive Maize Genotype

Khan, Ira / Vanaja, M / Sathish, P / Vagheera, P

Agricultural research. 2020 Sept., v. 9, no. 3

2020

Abstract: A CO₂-responsive maize (Zea mays L.) genotype, Harsha, was selected to assess its response to elevated CO₂ in successive generation. The selfed seeds harvested from elevated CO₂ (eCO₂) raised maize plants were sown in open-top chambers (OTCs) to raise ... ...

Abstract	A CO₂-responsive maize (Zea mays L.) genotype, Harsha, was selected to assess its response to elevated CO₂ in successive generation. The selfed seeds harvested from elevated CO₂ (eCO₂) raised maize plants were sown in open-top chambers (OTCs) to raise second generation (H-II) plants along with fresh seeds as first generation (H-I) under both ambient (400 ppm) and elevated (550 ppm) CO₂ (eCO₂) conditions. It was observed that second-generation (H-II) plants also showed improved performance under eCO₂ for morphological and physiological parameters. It is pertinent to mention that the H-II plants were more vigorous even under ambient CO₂ (aCO₂) condition as compared to H-I plants. The vigor of H-II plants was higher for majority of the morphological and biomass parameters under both aCO₂ and eCO₂. In H-II, the increased photosynthetic rate (Anet) was 6% and decrease in transpiration rate (Tr) was 49%, which resulted in significant increase (110%) in water use efficiency. This reveals that the elevated CO₂ improved the physiological performance of the maize plant over the generations. These results clearly elucidate that a C₄ crop like maize can respond to elevated CO₂ and higher response in subsequent generations may favor the crop resilience under changed climatic conditions.
Keywords	Zea mays ; agricultural research ; biomass ; carbon dioxide ; carbon dioxide enrichment ; corn ; genotype ; photosynthesis ; transpiration ; vigor ; water use efficiency
Language	English
Dates of publication	2020-09
Size	p. 310-315.
Publishing place	Springer India
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	2668414-7
ISSN	2249-7218 ; 2249-720X
ISSN (online)	2249-7218
ISSN	2249-720X
DOI	10.1007/s40003-019-00442-6
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Article ; Online: Brain Tumor Detection by Using Convolution Neural Network

Ayesha Samreen / Amtul Mohimin Taha / Yasa Vishwanath Reddy / Sathish P

International Journal of Online and Biomedical Engineering, Vol 16, Iss 13, Pp 58-

2020 Volume 69

Abstract: Nowadays, Biomedical technology plays a vital role in diagnosis and treatment of small to dangerous life threatening diseases and one of the most life threatening disease is Brain Tumor, which is the mass growth of abnormal cells in brain. Early ... ...

Abstract	Nowadays, Biomedical technology plays a vital role in diagnosis and treatment of small to dangerous life threatening diseases and one of the most life threatening disease is Brain Tumor, which is the mass growth of abnormal cells in brain. Early detection and treatment of it can save the human life by preventing the further growth of abnormal cells. Detection of it can be done by analysing the Magnetic Resonance Imaging (MRI) Scans. Accurate analysis of MRI Scans need to be done to detect the brain tumor and it can be achieved by using the algorithms of artificial neural networks, although human can detect manually but possibility to human errors is more and is time consuming. This paper proposes an effective algorithm model to predict brain tumor probability by using convolution neural networks. The algorithm includes image pre-processing in which noise is reduced using Gaussian filter and morphological operations. After that, images are normalized to scale fit. Batch normalization is added to the network to speed up the training. BRATS and Kaggle image dataset are used to train and evaluate the model to get maximised accuracy. Confusion matrix is used to evaluate the performance of the maximised model.
Keywords	convolution neural network (cnn) ; tensorflow ; keras ; brain tumor detection ; deep learning ; magnetic resonance imaging (mri) ; Computer applications to medicine. Medical informatics ; R858-859.7
Subject code	006
Language	English
Publishing date	2020-11-01T00:00:00Z
Publisher	International Association of Online Engineering (IAOE)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Polymicrobial Pyomyositis in a Patient with Suspected Myeloma on

Kalra, Simran / Damle, Nishikant Avinash / Gupta, Shreya Datta / Baghel, Vivek / Elavarasi, Arunmozhimaran / Sathish, Parkipandla

Indian journal of nuclear medicine : IJNM : the official journal of the Society of Nuclear Medicine, India

2022 Volume 37, Issue 2, Page(s) 192–193

Abstract: Pyomyositis is an infective involvement of systemic skeletal muscles. We discuss the case of 43-year-old male who presented with quadriparesis, anemia, and hypercalcemia, leading to suspicion of multiple myeloma, and on FDG PET-CT, incidentally, ... ...

Abstract	Pyomyositis is an infective involvement of systemic skeletal muscles. We discuss the case of 43-year-old male who presented with quadriparesis, anemia, and hypercalcemia, leading to suspicion of multiple myeloma, and on FDG PET-CT, incidentally, pyomyositis was found. FDG PET-CT thus helped in diagnosing an occult infection which helped in the treatment of the patient.
Language	English
Publishing date	2022-07-08
Publishing country	India
Document type	Journal Article
ZDB-ID	2181722-4
ISSN	0974-0244 ; 0972-3919
ISSN (online)	0974-0244
ISSN	0972-3919
DOI	10.4103/ijnm.ijnm_130_21
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Article ; Online: 2-(3-Indolyl)acetamides and their oxazoline analogues: Anticancer SAR study.

Aksenov, Dmitrii A / Smith, Jadyn L / Aksenov, Alexander V / Prityko, Lidiya A / Aksenov, Nicolai A / Kuzminov, Iliya K / Aleksandrova, Elena V / Sathish, Puppala / Mesa-Diaz, Nakya / Vernaza, Alexandra / Zhang, Angela / Du, Liqin / Kornienko, Alexander

Bioorganic & medicinal chemistry letters

2024 Volume 102, Page(s) 129681

Abstract: We previously studied 2-aryl-2-(3-indolyl)acetohydroxamates as potential agents against melanoma. These compounds were ineffective in a mouse melanoma xenograft model, most likely due to unfavorable metabolic properties, specifically due to ... ...

Abstract	We previously studied 2-aryl-2-(3-indolyl)acetohydroxamates as potential agents against melanoma. These compounds were ineffective in a mouse melanoma xenograft model, most likely due to unfavorable metabolic properties, specifically due to glucuronidation of the N-hydroxyl of the hydoxamic moiety. In the present work, we prepared a series of analogues, 2-aryl-2-(3-indolyl)acetamides and their oxazoline derivatives, which do not contain the N-hydroxyl group. We investigated the structure-activity relationship in both series of compounds and found that the 2-naphthyl is a preferred group at C-2 of the indole in the amide series, whereas the tetralin moiety is favorable in the same location in the oxazoline series. Overall, three compounds in the amide series have GI
MeSH term(s)	Humans ; Animals ; Mice ; Molecular Structure ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents/therapeutic use ; Acetamides/pharmacology ; Acetamides/therapeutic use ; Melanoma ; Structure-Activity Relationship
Chemical Substances	Antineoplastic Agents ; Acetamides
Language	English
Publishing date	2024-03-01
Publishing country	England
Document type	Journal Article
ZDB-ID	1063195-1
ISSN	1464-3405 ; 0960-894X
ISSN (online)	1464-3405
ISSN	0960-894X
DOI	10.1016/j.bmcl.2024.129681
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