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Article ; Online: In Vitro Risk Assessment of Dental Acid Erosion Caused by Long-Term Exposure to Oral Liquid Bandages.

Satou, Ryouichi / Sugihara, Naoki

2024 Volume 12, Issue 3

Abstract: Oral mucosa inflammation can cause severe pain and interfere with eating, reducing quality of life. However, few options for self-care are available. An oral liquid bandage forms a protective film over the affected area. We aimed to assess the acid ... ...

Abstract	Oral mucosa inflammation can cause severe pain and interfere with eating, reducing quality of life. However, few options for self-care are available. An oral liquid bandage forms a protective film over the affected area. We aimed to assess the acid erosion risk when a newly developed oral liquid bandage (ORAPLA) is accidentally deposited on teeth and to examine the relative acid erosion risk at multiple time points of the maximum recommended duration of continuous use. ORAPLA was applied to both enamel and dentin blocks from 45 bovine anterior mandibular teeth, and an acid challenge was performed in a simulated oral cavity with artificial saliva, with one exposure cycle lasting 6 h. The enamel showed substantial defects and a decrease in Vickers hardness after nine cycles, with no change in surface roughness. Dentin showed an increase in parenchymal defects and surface roughness and a trend toward decreased Vickers hardness with increasing exposure time. We found no significant acid corrosion in enamel after up to nine times the upper limit of normal use time or in dentin after up to six times the upper limit. We conclude that the acid erosion risk due to accidental attachment to teeth is low, and in the human oral cavity with salivary buffering and remineralization, likely even lower.
Language	English
Publishing date	2024-03-06
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2681351-8
ISSN	2304-6767 ; 2304-6767
ISSN (online)	2304-6767
ISSN	2304-6767
DOI	10.3390/dj12030070
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Fully Automated Bioreactor-Based pH-Cycling System for Demineralization: A Comparative Study with a Conventional Method.

Satou, Ryouichi / Miki, Naoko / Iwasaki, Miyu / Sugihara, Naoki

Materials (Basel, Switzerland)

2023 Volume 16, Issue 14

Abstract: This study aimed to develop an automated pH-cycling system using inexpensive commercial components that can replicate pH fluctuations in the oral cavity and salivary clearance to compare demineralization characteristics with the conventional method. The ... ...

Abstract	This study aimed to develop an automated pH-cycling system using inexpensive commercial components that can replicate pH fluctuations in the oral cavity and salivary clearance to compare demineralization characteristics with the conventional method. The study found that the newly developed cycle-1 group showed improved demineralization properties, including apparent lesion depth, surface roughness, Vickers hardness, mineral loss, and depth of demineralization, compared to the control group. Additionally, the cycle-2 group, which had a longer cycle interval, showed further improvements in the demineralization properties. This system can replicate the differences in dental damage caused by differences in meals, snacking frequencies, and lifestyle rhythms, making it useful in cariology, preventive dentistry research, and oral care product development. It can be constructed using inexpensive commercial products, significantly reducing research costs and improving reproducibility and fairness between different experimental facilities. The system can replicate lifestyle rhythms, such as meals, sleep, and oral clearance by saliva, making it an in vitro pseudo-oral cavity.
Language	English
Publishing date	2023-07-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma16144929
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Inhibition of Citric Acid-Induced Dentin Erosion by an Acidulated Phosphate Sodium Monofluorophosphate Solution.

Satou, Ryouichi / Ueno, Susumu / Kamijo, Hideyuki / Sugihara, Naoki

Materials (Basel, Switzerland)

2023 Volume 16, Issue 15

Abstract: Sodium monofluorophosphate ( ... ...

Abstract	Sodium monofluorophosphate (Na
Language	English
Publishing date	2023-07-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma16155230
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Development of Root Caries Prevention by Nano-Hydroxyapatite Coating and Improvement of Dentin Acid Resistance.

Iwasaki, Miyu / Satou, Ryouichi / Sugihara, Naoki

Materials (Basel, Switzerland)

2022 Volume 15, Issue 22

Abstract: There is no established method for optimizing the use of dentin to prevent root caries, which are increasing in the elderly population. This study aimed to develop a new approach for root caries prevention by focusing on bioapatite (BioHap), a new ... ...

Abstract	There is no established method for optimizing the use of dentin to prevent root caries, which are increasing in the elderly population. This study aimed to develop a new approach for root caries prevention by focusing on bioapatite (BioHap), a new biomaterial, combined with fluoride. Bovine dentin was used as a sample, and an acid challenge was performed in three groups: no fluoride (control group), acidulated phosphate fluoride treatment (APF group), and BioHap + APF treatment (BioHap group). After applying the new compound, the acid resistance of dentin was compared with that of APF alone. The BioHap group had fewer defects and an increased surface hardness than the APF group. The BioHap group had the smallest lesion depth and least mineral loss among all groups. Using a scanning electron microscope in the BioHap group showed the closure of dentinal tubules and a coating on the surface. The BioHap group maintained a coating and had higher acid resistance than the APF group. The coating prevents acid penetration, and the small particle size of BioHap and its excellent reactivity with fluoride are thought to have contributed to the improvement of acid resistance in dentin. Topical fluoride application using BioHap protects against root caries.
Language	English
Publishing date	2022-11-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma15228263
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Improved Enamel Acid Resistance Using Biocompatible Nano-Hydroxyapatite Coating Method.

Satou, Ryouichi / Iwasaki, Miyu / Kamijo, Hideyuki / Sugihara, Naoki

Materials (Basel, Switzerland)

2022 Volume 15, Issue 20

Abstract: In this study, we attempted to develop a dental caries prevention method using a bioapatite (BioHap), an eggshell-derived apatite with nanoparticle size and biocompatibility, with a high-concentration fluoride tooth surface application method. The enamel ...

Abstract	In this study, we attempted to develop a dental caries prevention method using a bioapatite (BioHap), an eggshell-derived apatite with nanoparticle size and biocompatibility, with a high-concentration fluoride tooth surface application method. The enamel acid resistance after the application of the proposed method was compared with that of a conventional topical application of fluoride using bovine tooth enamel as an example. The tooth samples were divided into three groups based on the preventive treatment applied, and an acid challenge was performed. The samples were evaluated for acid resistance using qualitative and quantitative analytical methods. The BioHap group demonstrated reduced enamel loss and improved micro-Vickers hardness, along with a thick coating layer, decreased reaction area depth, and decreased mineral loss value and lesion depth. The combination of BioHap with high-concentration fluoride led to the formation of a thick coating layer on the enamel surface and better suppression of demineralization than the conventional method, both qualitatively and quantitatively. The proposed biocompatible nano-hydroxyapatite coating method is expected to become a new standard for providing professional care to prevent dental caries.
Language	English
Publishing date	2022-10-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma15207171
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Delivery of Low-Diluted Toothpaste during Brushing Improves Enamel Acid Resistance.

Satou, Ryouichi / Shibata, Chikara / Takayanagi, Atsushi / Yamagishi, Atsushi / Birkhed, Dowen / Sugihara, Naoki

Materials (Basel, Switzerland)

2023 Volume 16, Issue 14

Abstract: Toothpaste viscosity decreases rapidly when diluted with saliva during brushing, potentially causing premature washout of high-risk caries areas and reducing the uptake of dental fluoride ions. However, no reports have examined the acid resistance of ... ...

Abstract	Toothpaste viscosity decreases rapidly when diluted with saliva during brushing, potentially causing premature washout of high-risk caries areas and reducing the uptake of dental fluoride ions. However, no reports have examined the acid resistance of enamel from the perspective of the toothpaste's physical properties. This study aimed to elucidate the impact of toothpaste dilution on the acid resistance of the enamel, using bovine enamel as the subject. Five diluted toothpaste groups were created: a control group without toothpaste, and 100% (1.00×), 67% (1.50×), 50% (2.00×), and 25% (4.00×) dilution groups. Acid resistance was evaluated through pH cycling after toothpaste application. The results revealed a significant increase in substantial defects, compared to 67% (1.50×) at dilutions of 50% (2.00×) or higher, accompanied by a decrease in Vickers hardness. Moreover, the mineral loss increased with dilution, and a significant difference was observed between 67% (1.50×) and 50% (2.00×) (
Language	English
Publishing date	2023-07-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma16145089
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Oral Health Status of Workers in Acid Exposure Environments in Japan: A Cross-sectional Study.

Suzuki, Seitaro / Yoshino, Koichi / Takayanagi, Atsushi / Onose, Yuki / Ohyama, Atsushi / Shibuya, Tomoaki / Satou, Ryouichi / Eguchi, Takako / Kamijo, Hideyuki / Sugihara, Naoki

The Bulletin of Tokyo Dental College

2024

Abstract: Although the working environment may play a role in dental acid erosion, few studies have been conducted on this in Japanese workers in recent years. The purpose of this study was to investigate oral health status, including dental erosion, in workers ... ...

Abstract	Although the working environment may play a role in dental acid erosion, few studies have been conducted on this in Japanese workers in recent years. The purpose of this study was to investigate oral health status, including dental erosion, in workers who may have been exposed to an acidic environment. The study participants were recruited by an online research company. Oral examinations and questionnaire surveys were conducted on this cohort in January 2023. A total of 144 participants were finally included. Workers exposed to an acidic environment showed more use of protective equipment and underwent more dental examinations at the workplace (p<0.001). Possible dental erosion was observed in only 3 out of the total of 144 participants (2.1%), however. No significant difference was observed in the number of teeth, caries experience, or dental erosion according to the number of years of acid exposure in the workplace. The results of this study suggest that acid exposure exerts a relatively small effect on the oral health status of workers in Japan.
Language	English
Publishing date	2024-05-15
Publishing country	Japan
Document type	Journal Article
ZDB-ID	968071-8
ISSN	0040-8891
ISSN	0040-8891
DOI	10.2209/tdcpublication.2023-0034
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Risk assessment of fluoride daily intake from preference beverage.

Satou, Ryouichi / Oka, Sari / Sugihara, Naoki

Journal of dental sciences

2020 Volume 16, Issue 1, Page(s) 220–228

Abstract: Background/purpose: Tea, coffee and alcohol beverages are called preference beverage and are drunk habitual and in large quantities. Therefore, there is a high possibility that a health risk is caused by the contained components, and risk assessment of ... ...

Abstract	Background/purpose: Tea, coffee and alcohol beverages are called preference beverage and are drunk habitual and in large quantities. Therefore, there is a high possibility that a health risk is caused by the contained components, and risk assessment of intake is essential. However, the risk assessment of fluoride intake from preference beverages has not been sufficiently performed. Materials and methods: This study estimated the daily fluoride intake from preference beverages by measuring the fluoride concentration of infusion liquid and measuring the total fluoride content by the microdiffusion method. In addition, Hazard Quotient (HQ) was calculated for children and adults to assess the risk of fluoride intake. Results: As a result of this study, tea was the highest in all sample species, the infusion of tea was 1.06-6.68 mg/L and the total fluoride content of tea was 47.05-291.98 mg/kg. Green tea showed the next highest value, 0.26-4.09 mg/L, 21.91-83.68 mg/kg. Herbal tea fluoride levels were 0.07-0.17 mg/L and 0.05-1.90 mg/kg, Unique tea was 0.03-0.60 mg/L and 0.03-32.37 mg/kg, Coffee was 0.03-0.15 mg/L and 0.04-0.64 mg/kg. Conclusion: The HQ values calculated from the average daily fluoride intake (DFI) of preference beverages were all within the safe range. Some products made from
Language	English
Publishing date	2020-06-08
Publishing country	Netherlands
Document type	Journal Article
ISSN	2213-8862
ISSN (online)	2213-8862
DOI	10.1016/j.jds.2020.05.023
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Improved Enamel Acid Resistance by Highly Concentrated Acidulated Phosphate Sodium Monofluorophosphate Solution.

Satou, Ryouichi / Yamagishi, Atsushi / Takayanagi, Atsushi / Iwasaki, Miyu / Kamijo, Hideyuki / Sugihara, Naoki

Materials (Basel, Switzerland)

2022 Volume 15, Issue 20

Abstract: Sodium monofluorophosphate (MFP) is a component of fluoride-containing dentifrices and is more biosafe than the conventional sodium fluoride (NaF). MFP can respond not only on the tooth surface layer but also deep into the enamel. We aim to confirm that ... ...

Abstract	Sodium monofluorophosphate (MFP) is a component of fluoride-containing dentifrices and is more biosafe than the conventional sodium fluoride (NaF). MFP can respond not only on the tooth surface layer but also deep into the enamel. We aim to confirm that high concentrations of acid phosphate MFP (AP-MFP, 9000 ppmF), used in professional care, could lead to a highly biosafe fluoride application method that acts through the deep enamel layers. Sample groups were respectively treated in vitro with NaF, acidulated phosphate fluoride (APF), MFP, and AP-MFP, and the samples were compared against an untreated group. Characterizations after fluoride application confirmed that MFP and AP-MFP treatments improved the acid resistance of enamel compared to that of conventional methods. Furthermore, the acid resistance of highly concentrated MFPs improved by using phosphoric acid. Although the acid resistance from the AP-MFP method is not as good as that using APF, AP-MFP can act both on the surface layer and deep into the enamel. Moreover, AP-MFP retains fluoride ions as much as APF does on the tooth surface. The proposed fluoride application method using AP-MFP introduces a dental treatment for acid resistance that is highly biosafe and penetrates deep layers of the enamel.
Language	English
Publishing date	2022-10-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma15207298
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Comparison of interproximal delivery and flow characteristics by dentifrice dilution and application of prepared toothpaste delivery technique.

Satou, Ryouichi / Yamagishi, Atsushi / Takayanagi, Atsushi / Suzuki, Seitaro / Birkhed, Dowen / Sugihara, Naoki

PloS one

2022 Volume 17, Issue 10, Page(s) e0276227

Abstract: In home care, the toothpaste technique, which can enhance the caries-preventive effect without changing the amount of dentifrice and fluoride ion concentration, is of great significance. This study aimed to construct a model and experimental system that ... ...

Abstract	In home care, the toothpaste technique, which can enhance the caries-preventive effect without changing the amount of dentifrice and fluoride ion concentration, is of great significance. This study aimed to construct a model and experimental system that reproduces the interdental part and to clarify the relationship between the change in dentifrice viscosity due to dilution and washout in the high-risk approximal area of caries. Additionally, the effectiveness of the toothpaste technique and appropriate devices for delivering dentifrice to the interdental area at a low dilution were investigated. Diluted toothpaste samples were prepared (: ×1.00, ×1.25, ×1.50, ×1.75, ×2.00, ×3.00, and ×4.00). An acrylic interproximal model was created for this experiment. The flow characteristics and viscosity by dentifrice dilution were measured. In the case of low dilution of 57% (1.75×) or more, it was shown that the dentifrice in the high-risk area may be washed out early because of the decrease in viscosity, and the caries-preventive effect may be reduced. It was also suggested that to keep the dentifrice in the interdental area for 120 s at the end of brushing, a dilution must be devised to a concentration of at least 50% (2.00×). The prepared toothpaste delivery (PTD) method of delivering dentifrice to the interdental area while maintaining it at a low dilution is an effective toothpaste technique in terms of dentifrice dilution and viscosity. The use of finger brushes in the PTD method could increase the efficiency of dentifrice delivery.
MeSH term(s)	Dentifrices ; Fluorides ; Indicator Dilution Techniques ; Sodium Fluoride ; Toothbrushing/methods ; Toothpastes
Chemical Substances	Dentifrices ; Toothpastes ; Sodium Fluoride (8ZYQ1474W7) ; Fluorides (Q80VPU408O)
Language	English
Publishing date	2022-10-17
Publishing country	United States
Document type	Journal Article
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0276227
Database	MEDical Literature Analysis and Retrieval System OnLINE

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