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Article ; Online: Simultaneous Surface-Enhanced Raman Scattering with a Kerr Gate for Fluorescence Suppression.

Cabello, Gema / Sazanovich, Igor V / Siachos, Ioannis / Bilton, Matthew / Mehdi, Beata L / Neale, Alex R / Hardwick, Laurence J

The journal of physical chemistry letters

2024 Volume 15, Issue 2, Page(s) 608–615

Abstract: The combination of surface-enhanced and Kerr-gated Raman spectroscopy for the enhancement of the Raman signal and suppression of fluorescence is reported. Surface-enhanced Raman scattering (SERS)-active gold substrates were demonstrated for the expansion ...

Abstract	The combination of surface-enhanced and Kerr-gated Raman spectroscopy for the enhancement of the Raman signal and suppression of fluorescence is reported. Surface-enhanced Raman scattering (SERS)-active gold substrates were demonstrated for the expansion of the surface generality of optical Kerr-gated Raman spectroscopy, broadening its applicability to the study of analytes that show a weak Raman signal in highly fluorescent media under (pre)resonant conditions. This approach is highlighted by the well-defined spectra of rhodamine 6G, Nile red, and Nile blue. The Raman spectra of fluorescent dyes were obtained only when SERS-active substrates were used in combination with the Kerr gate. To achieve enhancement of the weaker Raman scattering, Au films with different roughnesses or Au-core-shell-isolated nanoparticles (SHINs) were used. The use of SHINs enabled measurement of fluorescent dyes on non-SERS-active, optically flat Au, Cu, and Al substrates.
Language	English
Publishing date	2024-01-10
Publishing country	United States
Document type	Journal Article
ISSN	1948-7185
ISSN (online)	1948-7185
DOI	10.1021/acs.jpclett.3c02926
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Unraveling Excited State Dynamics of a Single-Stranded DNA-Assembled Conjugated Polyelectrolyte.

Nicolaidou, Eliana / Parker, Anthony W / Sazanovich, Igor V / Towrie, Michael / Hayes, Sophia C

The journal of physical chemistry letters

2023 Volume 14, Issue 44, Page(s) 9794–9803

Abstract: Conformational templating of conjugated polyelectrolytes with single-stranded DNAs (ssDNAs) has the prospect of tailoring excited state dynamics for specific optoelectronic applications. We use ultrafast time-resolved infrared spectroscopy to study the ... ...

Abstract	Conformational templating of conjugated polyelectrolytes with single-stranded DNAs (ssDNAs) has the prospect of tailoring excited state dynamics for specific optoelectronic applications. We use ultrafast time-resolved infrared spectroscopy to study the photophysics of a cationic polythiophene assembled with different ssDNAs, inducing distinct conformations (flexible disordered structures vs more rigid complexes with increased backbone planarity). Intrachain polarons are always produced upon selective excitation of the polymer, the extent being dependent on backbone torsional order. Polaron formation and decay were monitored through evolution of IR-active vibrational modes that interfere with mid-IR polaron electronic absorption giving rise to Fano-antiresonances. Selective UV excitation of ssDNAs revealed that stacking interactions between thiophene rings and nucleic acid bases can promote the formation of an intermolecular charge transfer complex. The findings inform designers of functional conjugated polymers by identifying that involvement of the scaffold in the photophysics needs to be considered when developing such structures for optoelectronic applications.
MeSH term(s)	DNA, Single-Stranded ; Polyelectrolytes ; Spectrophotometry, Infrared
Chemical Substances	DNA, Single-Stranded ; Polyelectrolytes
Language	English
Publishing date	2023-10-26
Publishing country	United States
Document type	Journal Article
ISSN	1948-7185
ISSN (online)	1948-7185
DOI	10.1021/acs.jpclett.3c01803
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Article: Lithium Insertion into Graphitic Carbon Observed via Operando Kerr-Gated Raman Spectroscopy Enables High State of Charge Diagnostics.

Neale, Alex R / Milan, David C / Braga, Filipe / Sazanovich, Igor V / Hardwick, Laurence J

ACS energy letters

2022 Volume 7, Issue 8, Page(s) 2611–2618

Abstract: Monitoring the precise lithium inventory of the graphitic carbon electrode within the Li-ion battery, in order to assess cell aging, has remained challenging. Herein, operando electrochemical Kerr-gated Raman spectroscopy measurements on microcrystalline ...

Abstract	Monitoring the precise lithium inventory of the graphitic carbon electrode within the Li-ion battery, in order to assess cell aging, has remained challenging. Herein, operando electrochemical Kerr-gated Raman spectroscopy measurements on microcrystalline graphite during complete lithium insertion and extraction are reported and compared to conventional continuous-wave Raman microscopy. Suppression of the fluorescence emission signals via use of the Kerr gate enabled the measurement of the Raman graphitic bands of highly lithiated graphite where 0.5 ≤
Language	English
Publishing date	2022-07-18
Publishing country	United States
Document type	Journal Article
ISSN	2380-8195
ISSN	2380-8195
DOI	10.1021/acsenergylett.2c01120
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Article ; Online: Tuning of B

Camacho, Ines S / Wall, Emma / Sazanovich, Igor V / Gozzard, Emma / Towrie, Mike / Hunt, Neil T / Hay, Sam / Jones, Alex R

Chemical communications (Cambridge, England)

2023 Volume 59, Issue 87, Page(s) 13014–13017

Abstract: Time-resolved infrared spectroscopy reveals the flow of electron density through coenzyme ... ...

Abstract	Time-resolved infrared spectroscopy reveals the flow of electron density through coenzyme B
MeSH term(s)	Photochemistry ; Cobamides ; Bacteria
Chemical Substances	cobamamide (F0R1QK73KB) ; Cobamides
Language	English
Publishing date	2023-10-31
Publishing country	England
Document type	Journal Article
ZDB-ID	1472881-3
ISSN	1364-548X ; 1359-7345 ; 0009-241X
ISSN (online)	1364-548X
ISSN	1359-7345 ; 0009-241X
DOI	10.1039/d3cc03900e
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Article ; Online: Characterization of the Reversible Intersystem Crossing Dynamics of Organic Photocatalysts Using Transient Absorption Spectroscopy and Time-Resolved Fluorescence Spectroscopy.

Whitaker, William / Sazanovich, Igor V / Kwon, Yonghwan / Jeon, Woojin / Kwon, Min Sang / Orr-Ewing, Andrew J

The journal of physical chemistry. A

2023 Volume 127, Issue 51, Page(s) 10775–10788

Abstract: Thermally activated delayed fluorescence (TADF) emitters are molecules of interest as homogeneous organic photocatalysts (OPCs) for photoredox chemistry. Here, three classes of OPC candidates are studied in dichloromethane (DCM) or N,N-dimethylformamide ( ...

Abstract	Thermally activated delayed fluorescence (TADF) emitters are molecules of interest as homogeneous organic photocatalysts (OPCs) for photoredox chemistry. Here, three classes of OPC candidates are studied in dichloromethane (DCM) or N,N-dimethylformamide (DMF) solutions, using transient absorption spectroscopy and time-resolved fluorescence spectroscopy. These OPCs are benzophenones with either carbazole (2Cz-BP and 2tCz-BP) or phenoxazine/phenothiazine (2PXZ-BP and 2PTZ-BP) appended groups and the dicyanobenzene derivative 4DP-IPN. Dual lifetimes of the S
Language	English
Publishing date	2023-12-14
Publishing country	United States
Document type	Journal Article
ISSN	1520-5215
ISSN (online)	1520-5215
DOI	10.1021/acs.jpca.3c04780
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Article ; Online: Photoinduced Electron Transfer from a 1,4,5,6-Tetrahydro Nicotinamide Adenine Dinucleotide (Phosphate) Analogue to Oxidized Flavin in an Ene-Reductase Flavoenzyme.

Speirs, Magnus / Hardman, Samantha J O / Iorgu, Andreea I / Johannissen, Linus O / Heyes, Derren J / Scrutton, Nigel S / Sazanovich, Igor V / Hay, Sam

The journal of physical chemistry letters

2023 Volume 14, Issue 13, Page(s) 3236–3242

Abstract: Recent reports have described the use of ene-reductase flavoenzymes to catalyze non-natural photochemical reactions. These studies have focused on using reduced flavoenzyme, yet oxidized flavins have superior light harvesting properties. In a binary ... ...

Abstract	Recent reports have described the use of ene-reductase flavoenzymes to catalyze non-natural photochemical reactions. These studies have focused on using reduced flavoenzyme, yet oxidized flavins have superior light harvesting properties. In a binary complex of the oxidized ene-reductase pentaerythritol tetranitrate reductase with the nonreactive nicotinamide coenzyme analogs 1,4,5,6-tetrahydro NAD(P)H, visible photoexcitation of the flavin mononucleotide (FMN) leads to one-electron transfer from the NAD(P)H
MeSH term(s)	Oxidoreductases/chemistry ; NAD/chemistry ; NADP ; Oxidation-Reduction ; Electrons ; Flavins/chemistry ; Phosphates ; Kinetics
Chemical Substances	Oxidoreductases (EC 1.-) ; NAD (0U46U6E8UK) ; NADP (53-59-8) ; Flavins ; Phosphates
Language	English
Publishing date	2023-03-27
Publishing country	United States
Document type	Journal Article
ISSN	1948-7185
ISSN (online)	1948-7185
DOI	10.1021/acs.jpclett.3c00176
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Article ; Online: The Transition from Unfolded to Folded G-Quadruplex DNA Analyzed and Interpreted by Two-Dimensional Infrared Spectroscopy.

Soenarjo, A Larasati / Lan, Zhihao / Sazanovich, Igor V / Chan, Yee San / Ringholm, Magnus / Jha, Ajay / Klug, David R

Journal of the American Chemical Society

2023 Volume 145, Issue 36, Page(s) 19622–19632

Abstract: A class of DNA folds/structures known collectively as G-quadruplexes (G4) commonly forms in guanine-rich areas of genomes. G4-DNA is thought to have a functional role in the regulation of gene transcription and telomerase-mediated telomere maintenance ... ...

Abstract	A class of DNA folds/structures known collectively as G-quadruplexes (G4) commonly forms in guanine-rich areas of genomes. G4-DNA is thought to have a functional role in the regulation of gene transcription and telomerase-mediated telomere maintenance and, therefore, is a target for drugs. The details of the molecular interactions that cause stacking of the guanine-tetrads are not well-understood, which limits a rational approach to the drugability of G4 sequences. To explore these interactions, we employed electron-vibration-vibration two-dimensional infrared (EVV 2DIR) spectroscopy to measure extended vibrational coupling spectra for a parallel-stranded G4-DNA formed by the Myc2345 nucleotide sequence. We also tracked the structural changes associated with G4-folding as a function of K
MeSH term(s)	G-Quadruplexes ; Spectrophotometry, Infrared ; DNA ; Density Functional Theory ; Electrons
Chemical Substances	DNA (9007-49-2)
Language	English
Publishing date	2023-08-30
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021/jacs.3c04044
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Article: Towards understanding non-equivalence of α and β subunits within human hemoglobin in conformational relaxation and molecular oxygen rebinding.

Lepeshkevich, Sergei V / Sazanovich, Igor V / Parkhats, Marina V / Gilevich, Syargey N / Dzhagarov, Boris M

Chemical science

2021 Volume 12, Issue 20, Page(s) 7033–7047

Abstract: Picosecond to millisecond laser time-resolved transient absorption spectroscopy was used to study molecular oxygen ( ... ...

Abstract	Picosecond to millisecond laser time-resolved transient absorption spectroscopy was used to study molecular oxygen (O
Language	English
Publishing date	2021-04-15
Publishing country	England
Document type	Journal Article
ZDB-ID	2559110-1
ISSN	2041-6539 ; 2041-6520
ISSN (online)	2041-6539
ISSN	2041-6520
DOI	10.1039/d1sc00712b
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Article ; Online: Nonadiabatic excited-state dynamics of ReCl(CO)

Šrut, Adam / Mai, Sebastian / Sazanovich, Igor V / Heyda, Jan / Vlček, Antonín / González, Leticia / Záliš, Stanislav

Physical chemistry chemical physics : PCCP

2022 Volume 24, Issue 42, Page(s) 25864–25877

Abstract: We present a study of excited-states relaxation of the complex ReCl(CO) ...

Abstract	We present a study of excited-states relaxation of the complex ReCl(CO)
Language	English
Publishing date	2022-11-02
Publishing country	England
Document type	Journal Article
ZDB-ID	1476244-4
ISSN	1463-9084 ; 1463-9076
ISSN (online)	1463-9084
ISSN	1463-9076
DOI	10.1039/d2cp02981b
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Article ; Online: Solving the Puzzle of Unusual Excited-State Proton Transfer in 2,5-Bis(6-methyl-2-benzoxazolyl)phenol.

Dobkowski, Jacek / Kijak, Michał / Gawinkowski, Sylwester / Karpiuk, Elena / Pietrzak, Mariusz / Sazanovich, Igor V / Waluk, Jacek

The journal of physical chemistry. A

2022 Volume 126, Issue 11, Page(s) 1823–1836

Abstract: 2,5-Bis(6-methyl-2-benzoxazolyl)phenol ( ...

Abstract	2,5-Bis(6-methyl-2-benzoxazolyl)phenol (
Language	English
Publishing date	2022-03-14
Publishing country	United States
Document type	Journal Article
ISSN	1520-5215
ISSN (online)	1520-5215
DOI	10.1021/acs.jpca.1c10030
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