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  1. Article ; Online: Lipopolysaccharide Is a 4-Aminoarabinose Donor to Exogenous Polyisoprenyl Phosphates through the Reverse Reaction of the Enzyme ArnT.

    Scarbrough, Beth A / Eade, Colleen R / Reid, Amanda J / Williams, Tiffany C / Troutman, Jerry M

    ACS omega

    2021  Volume 6, Issue 39, Page(s) 25729–25741

    Abstract: Modification of the lipid A portion of LPS with cationic monosaccharides provides resistance to polymyxins, which are often employed as a last resort to treat multidrug-resistant bacterial infections. Here, we describe the use of fluorescent ... ...

    Abstract Modification of the lipid A portion of LPS with cationic monosaccharides provides resistance to polymyxins, which are often employed as a last resort to treat multidrug-resistant bacterial infections. Here, we describe the use of fluorescent polyisoprenoids, liquid chromatography-mass spectrometry, and bacterial genetics to probe the activity of membrane-localized proteins that utilize the 55-carbon lipid carrier bactoprenyl phosphate (BP). We have discovered that a substantial background reaction occurs when B-strain
    Language English
    Publishing date 2021-09-20
    Publishing country United States
    Document type Journal Article
    ISSN 2470-1343
    ISSN (online) 2470-1343
    DOI 10.1021/acsomega.1c04036
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: General Utilization of Fluorescent Polyisoprenoids with Sugar Selective Phosphoglycosyltransferases

    Reid, Amanda J / Scarbrough, Beth A / Williams, Tiffany C / Gates, Claire E / Eade, Colleen R / Troutman, Jerry M

    Biochemistry. 2019 Dec. 26, v. 59, no. 4

    2019  

    Abstract: The protective surfaces of bacteria are comprised of polysaccharides and are involved in host invasion and colonization, host immune system evasion, and antibacterial resistance. A major barrier to our fundamental understanding of these complex surface ... ...

    Abstract The protective surfaces of bacteria are comprised of polysaccharides and are involved in host invasion and colonization, host immune system evasion, and antibacterial resistance. A major barrier to our fundamental understanding of these complex surface polysaccharides lies in the tremendous diversity in glycan composition among bacterial species. The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly. Because of the ubiquity of bactoprenyl phosphate in these critical processes, molecular probes appended to this lipid carrier simplify identification of enzymatic roles during polysaccharide bioassembly. A limited number of these probes exist in the literature or have been assessed with such pathways, and the limits of their use are not currently known. Herein, we devise an efficient method for producing fluorescently modified bactoprenyl probes. We further expand our previous efforts utilizing 2-nitrileaniline and additionally prepare nitrobenzoxadizol-tagged bactoprenyl phosphate for the first time. We then assess the enzyme promiscuity of these two probes utilizing four well-characterized initiating phosphoglycosyltransferases: CPS2E (Streptococcus pneumoniae), WbaP (Salmonella enterica), WecA (Escherichia coli), and WecP (Aeromonas hydrophilia). Both probes serve as substrates for these enzymes and could be readily used to investigate a wide range of bacterial glycoassembly pathways. Interestingly, we have also identified unique solubility requirements for the nitrobenzoxadizol moiety for efficient enzymatic utilization that was not observed for the 2-nitrileaniline.
    Keywords Aeromonas ; Escherichia coli ; Salmonella enterica ; Streptococcus pneumoniae ; antibiotic resistance ; bacteria ; enzymes ; fluorescence ; immune system ; lipids ; literature ; moieties ; phosphates ; polysaccharides ; solubility ; sugars ; surfaces
    Language English
    Dates of publication 2019-1226
    Size p. 615-626.
    Publishing place American Chemical Society
    Document type Article
    Note NAL-light
    ZDB-ID 1108-3
    ISSN 1520-4995 ; 0006-2960
    ISSN (online) 1520-4995
    ISSN 0006-2960
    DOI 10.1021/acs.biochem.9b01026
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 217: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: General Utilization of Fluorescent Polyisoprenoids with Sugar Selective Phosphoglycosyltransferases.

    Reid, Amanda J / Scarbrough, Beth A / Williams, Tiffany C / Gates, Claire E / Eade, Colleen R / Troutman, Jerry M

    Biochemistry

    2020  Volume 59, Issue 4, Page(s) 615–626

    Abstract: The protective surfaces of bacteria are comprised of polysaccharides and are involved in host invasion and colonization, host immune system evasion, and antibacterial resistance. A major barrier to our fundamental understanding of these complex surface ... ...

    Abstract The protective surfaces of bacteria are comprised of polysaccharides and are involved in host invasion and colonization, host immune system evasion, and antibacterial resistance. A major barrier to our fundamental understanding of these complex surface polysaccharides lies in the tremendous diversity in glycan composition among bacterial species. The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly. Because of the ubiquity of bactoprenyl phosphate in these critical processes, molecular probes appended to this lipid carrier simplify identification of enzymatic roles during polysaccharide bioassembly. A limited number of these probes exist in the literature or have been assessed with such pathways, and the limits of their use are not currently known. Herein, we devise an efficient method for producing fluorescently modified bactoprenyl probes. We further expand our previous efforts utilizing 2-nitrileaniline and additionally prepare nitrobenzoxadizol-tagged bactoprenyl phosphate for the first time. We then assess the enzyme promiscuity of these two probes utilizing four well-characterized initiating phosphoglycosyltransferases: CPS2E (
    MeSH term(s) Bacterial Proteins/chemistry ; Cloning, Molecular/methods ; Escherichia coli/metabolism ; Polyisoprenyl Phosphates/chemistry ; Polyprenols/chemistry ; Salmonella enterica/metabolism ; Streptococcus pneumoniae/metabolism ; Sugars
    Chemical Substances Bacterial Proteins ; Polyisoprenyl Phosphates ; Polyprenols ; Sugars ; undecaprenyl phosphate (25126-51-6)
    Language English
    Publishing date 2020-01-07
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 1108-3
    ISSN 1520-4995 ; 0006-2960
    ISSN (online) 1520-4995
    ISSN 0006-2960
    DOI 10.1021/acs.biochem.9b01026
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 217: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Making the Enterobacterial Common Antigen Glycan and Measuring Its Substrate Sequestration.

    Eade, Colleen R / Wallen, Timothy W / Gates, Claire E / Oliverio, Cassidy L / Scarbrough, Beth A / Reid, Amanda J / Jorgenson, Matthew A / Young, Kevin D / Troutman, Jerry M

    ACS chemical biology

    2021  Volume 16, Issue 4, Page(s) 691–700

    Abstract: The enterobacterial common antigen (ECA), a three-sugar repeat unit polysaccharide produced by Enterobacteriaceae family members, impacts bacterial outer membrane permeability, and its biosynthesis affects the glycan landscape of the organism. ECA ... ...

    Abstract The enterobacterial common antigen (ECA), a three-sugar repeat unit polysaccharide produced by Enterobacteriaceae family members, impacts bacterial outer membrane permeability, and its biosynthesis affects the glycan landscape of the organism. ECA synthesis impacts the production of other polysaccharides by reducing the availability of shared substrates, the most notable of which is the 55-carbon polyisoprenoid bactoprenyl phosphate (BP), which serves as a carrier for the production of numerous bacterial glycans including ECA, peptidoglycan, O-antigen, and more. Here, using a combination of
    MeSH term(s) Antigens, Bacterial/metabolism ; Chromatography, Liquid ; Mass Spectrometry ; Polysaccharides/metabolism ; Substrate Specificity
    Chemical Substances Antigens, Bacterial ; Polysaccharides ; enterobacterial common antigen
    Language English
    Publishing date 2021-03-19
    Publishing country United States
    Document type Journal Article
    ISSN 1554-8937
    ISSN (online) 1554-8937
    DOI 10.1021/acschembio.0c00983
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Influence of Cationic

    Hurst, Alexandra N / Scarbrough, Beth / Saleh, Roa / Hovey, Jessica / Ari, Farideh / Goyal, Shreya / Chi, Richard J / Troutman, Jerry M / Vivero-Escoto, Juan L

    International journal of molecular sciences

    2019  Volume 20, Issue 1

    Abstract: Photodynamic inactivation (PDI) is a non-antibiotic option for the treatment of infectious diseases. Although Gram-positive bacteria have been shown to be highly susceptible to PDI, the inactivation of Gram-negative bacteria has been more challenging due ...

    Abstract Photodynamic inactivation (PDI) is a non-antibiotic option for the treatment of infectious diseases. Although Gram-positive bacteria have been shown to be highly susceptible to PDI, the inactivation of Gram-negative bacteria has been more challenging due to the impermeability properties of the outer membrane. In the present study, a series of photosensitizers which contain one to four positive charges (
    MeSH term(s) Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/pharmacology ; Biological Transport ; Cations/chemistry ; Cell Membrane/drug effects ; Escherichia coli/drug effects ; Escherichia coli/radiation effects ; Photosensitizing Agents/chemistry ; Photosensitizing Agents/pharmacology ; Photosensitizing Agents/radiation effects ; Porphyrins/chemistry ; Porphyrins/pharmacology ; Porphyrins/radiation effects ; Quantitative Structure-Activity Relationship ; Static Electricity ; Ultraviolet Rays
    Chemical Substances Anti-Bacterial Agents ; Cations ; Photosensitizing Agents ; Porphyrins
    Language English
    Publishing date 2019-01-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms20010134
    Database MEDical Literature Analysis and Retrieval System OnLINE

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