Article ; Online: Development of a molecular marker for the Pi1 gene based on the association of the SNAP protocol with the touch-up gradient amplification method.
Journal of microbiological methods
2023 Volume 214, Page(s) 106845
Abstract: Genetic resistance is the most effective and eco-friendly approach to combat rice blast. The application of resistance genes may be facilitated by the availability of molecular markers that allow marker-assisted selection during the breeding process. The ...
| Abstract | Genetic resistance is the most effective and eco-friendly approach to combat rice blast. The application of resistance genes may be facilitated by the availability of molecular markers that allow marker-assisted selection during the breeding process. The Pi1 gene, considered to be a broad-spectrum resistance gene, might contribute to enhancing resistance to rice blast, but it lacks a suitable marker that can be used. In this study, we investigated nucleotide polymorphism in the Pik locus and combined the SNAP protocol with the touch-up gradient amplification method to develop a SNAP marker. The Pi1 SNAP marker could distinguish Pi1 from Pik alleles, and when used for screening a germplasm bank and an F2 population, it consistently identified germplasms carrying the Pi1 gene. The P1 SNAP marker offers as advantages to involve only the presence/absence analysis of PCR amplicons resolved on an agarose gel. |
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| MeSH term(s) | Alleles ; Disease Resistance/genetics ; Genetic Markers/genetics ; Oryza/genetics ; Plant Diseases ; Polymorphism, Genetic |
| Chemical Substances | Genetic Markers |
| Language | English |
| Publishing date | 2023-10-17 |
| Publishing country | Netherlands |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 604916-3 |
| ISSN | 1872-8359 ; 0167-7012 |
| ISSN (online) | 1872-8359 |
| ISSN | 0167-7012 |
| DOI | 10.1016/j.mimet.2023.106845 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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