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  1. Book ; Online ; E-Book: Allergy prevention and exacerbation

    Schmidt-Weber, Carsten B.

    the paradox of microbial impact on the immune system

    (Birkhäuser advances in infectious diseases)

    2017  

    Author's details Carsten B. Schmidt-Weber editor
    Series title Birkhäuser advances in infectious diseases
    Language English
    Size 1 Online-Ressource (vi, 248 Seiten), Illustrationen
    Publisher Springer
    Publishing place Cham
    Publishing country Switzerland
    Document type Book ; Online ; E-Book
    Remark Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
    HBZ-ID HT019702236
    ISBN 978-3-319-69968-4 ; 9783319699677 ; 3-319-69968-7 ; 3319699679
    DOI 10.1007/978-3-319-69968-4
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

    Full text online

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  2. Article ; Online: In Vitro Study of TLR4-NLRP3-Inflammasome Activation in Innate Immune Response.

    Mezzasoma, Letizia / Schmidt-Weber, Carsten B / Fallarino, Francesca

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2700, Page(s) 163–176

    Abstract: Toll-like receptors (TLRs) are pivotal players in mediating immune responses. TLR4 is the main receptor for LPS, a strong activator of immune cells. LPS/TLR4-dependent pathway, by inducing NF-κB activation, is responsible for the release of several ... ...

    Abstract Toll-like receptors (TLRs) are pivotal players in mediating immune responses. TLR4 is the main receptor for LPS, a strong activator of immune cells. LPS/TLR4-dependent pathway, by inducing NF-κB activation, is responsible for the release of several mediators, including IL-1β, one of the most powerful cytokines deeply involved in inflammatory and immune responses. The same pathway is also involved in NLRP3-inflammasome activation, essential for IL-1β maturation. NLRP3 is a major component of innate immune responses, being a crucial player of host immune defense against virus, bacterial, or fungal infections. NLRP3-inflammasome and IL-1β hyperactivation have been associated to the pathogenesis of a wide range of disorders and represent therapeutic targets for the development of new treatments of inflammasome-driven inflammatory and autoimmune diseases.Here, we describe an in vitro protocol to induce LPS/TLR4-dependent NLRP3-inflammasome/IL-1β activation in immune cells, in order to provide a useful assay to study the efficacy of different anti-inflammatory/immune-modulatory agents.
    MeSH term(s) Inflammasomes ; NLR Family, Pyrin Domain-Containing 3 Protein ; Toll-Like Receptor 4 ; Lipopolysaccharides/pharmacology ; Immunity, Innate ; Radiopharmaceuticals
    Chemical Substances Inflammasomes ; NLR Family, Pyrin Domain-Containing 3 Protein ; Toll-Like Receptor 4 ; Lipopolysaccharides ; Radiopharmaceuticals
    Language English
    Publishing date 2023-08-21
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3366-3_9
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  3. Article ; Online: Dosing intact birch pollen grains at the air-liquid interface (ALI) to the immortalized human bronchial epithelial cell line BEAS-2B.

    Candeias, Joana / Schmidt-Weber, Carsten B / Buters, Jeroen

    PloS one

    2021  Volume 16, Issue 11, Page(s) e0259914

    Abstract: In real life, humans are exposed to whole pollen grains at the air epithelial barrier. We developed a system for in vitro dosing of whole pollen grains at the Air-Liquid Interface (ALI) and studied their effect on the immortalized human bronchial ... ...

    Abstract In real life, humans are exposed to whole pollen grains at the air epithelial barrier. We developed a system for in vitro dosing of whole pollen grains at the Air-Liquid Interface (ALI) and studied their effect on the immortalized human bronchial epithelial cell line BEAS-2B. Pollen are sticky and large particles. Dosing pollen needs resuspension of single particles rather than clusters, and subsequent transportation to the cells with little loss to the walls of the instrumentation i.e. in a straight line. To avoid high speed impacting insults to cells we chose sedimentation by gravity as a delivery step. Pollen was resuspended into single particles by pressured air. A pollen dispersion unit including PTFE coating of the walls and reduced air pressure limited impaction loss to the walls. The loss of pollen to the system was still about 40%. A linear dose effect curve resulted in 327-2834 pollen/cm2 (± 6.1%), the latter concentration being calculated as the amount deposited on epithelial cells on high pollen days. After whole pollen exposure, the largest differential gene expression at the transcriptomic level was late, about 7 hours after exposure. Inflammatory and response to stimulus related genes were up-regulated. We developed a whole pollen exposure air-liquid interface system (Pollen-ALI), in which cells can be gently and reliably dosed.
    MeSH term(s) Betula/chemistry ; Bronchi/chemistry ; Bronchi/cytology ; Bronchi/drug effects ; Cell Line ; Cytokines/genetics ; Epithelial Cells/chemistry ; Epithelial Cells/cytology ; Epithelial Cells/drug effects ; Fractionation, Field Flow ; Gene Expression Profiling/methods ; Gene Expression Regulation ; Humans ; Interleukin-17/genetics ; Interleukin-33/genetics ; Pollen/adverse effects ; Pollen/immunology
    Chemical Substances Cytokines ; IL25 protein, human ; IL33 protein, human ; Interleukin-17 ; Interleukin-33 ; TSLP protein, human
    Language English
    Publishing date 2021-11-16
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0259914
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Book ; Thesis: Untersuchungen zum Wirkungsmechanismus von Anti-T-Zell- und Anti-Makrophagen-Therapeutika in der Adjuvansarthritis der Ratte

    Schmidt-Weber, Carsten B.

    1996  

    Author's details vorgelegt von Carsten B. Schmidt-Weber
    Language German
    Size 133 S. : Ill., graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Erlangen-Nürnberg, Univ., Diss., 1996
    HBZ-ID HT007370798
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    96 D 3721 order for loan Magazin

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  5. Article ; Online: Predicting Success of Allergen-Specific Immunotherapy.

    Zissler, Ulrich M / Schmidt-Weber, Carsten B

    Frontiers in immunology

    2020  Volume 11, Page(s) 1826

    Abstract: The immune response to antigens is a key aspect of immunology, as it provides opportunities for therapeutic intervention. However, the induction of immunological tolerance is an evolving area that is still not sufficiently understood. Allergen ... ...

    Abstract The immune response to antigens is a key aspect of immunology, as it provides opportunities for therapeutic intervention. However, the induction of immunological tolerance is an evolving area that is still not sufficiently understood. Allergen immunotherapy (AIT) is a disease-modulating therapy available for immunoglobulin E (IgE)-mediated airway diseases such as allergic rhinitis or allergic asthma. This disease-modifying effect is not only antigen driven but also antigen specific. The specificity and also the long-lasting, often life-long symptom reduction make the therapy attractive for patients. Additionally, the chance to prevent the onset of asthma by treating allergic rhinitis with AIT is important. The mechanism and, in consequence, therapy guiding biomarker are still in its infancy. Recent studies demonstrated that the interaction of T, B, dendritic, and epithelial cells and macrophages are individually contributing to clinical tolerance and therefore underline the need for a system to monitor the progress and success of AIT. As clinical improvement is often accompanied by decreases in numbers of effector cells in the tissue, analyses of cellular responses and cytokine pattern provide a good insight into the mechanisms of AIT. The suppression of type-2 immunity is accompanied by decreased levels of type-2 mediators such as epithelial CCL-26 and interleukin (IL)-4, IL-13 produced by T cells that are constituting the immune memory and are increasingly controlled by regulatory T and B cells following AIT. Immune tolerance is also associated with increased production of type-1 mediators like interferon-gamma, tissue-homeostating factors like indoleamine 2,3-dioxygenase (IDO) expressed by macrophages and dendritic cells. Although these individual genes were convincingly demonstrated to play a role immune tolerance, they do not predict therapy outcomes of AIT on an individual level. Therefore, combinations or ratios of gene expression levels are a promising way to achieve predictive value and definition of helpful biomarker.
    MeSH term(s) Allergens/adverse effects ; Allergens/immunology ; Allergens/therapeutic use ; Biomarkers/metabolism ; Cytokines/metabolism ; Desensitization, Immunologic/adverse effects ; Humans ; Hypersensitivity/diagnosis ; Hypersensitivity/immunology ; Hypersensitivity/metabolism ; Hypersensitivity/therapy ; Immune Tolerance ; Inflammation Mediators/metabolism ; Predictive Value of Tests ; Treatment Outcome
    Chemical Substances Allergens ; Biomarkers ; Cytokines ; Inflammation Mediators
    Language English
    Publishing date 2020-08-25
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2020.01826
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Role of Respiratory Epithelial Cells in Allergic Diseases.

    Jakwerth, Constanze A / Ordovas-Montanes, Jose / Blank, Simon / Schmidt-Weber, Carsten B / Zissler, Ulrich M

    Cells

    2022  Volume 11, Issue 9

    Abstract: The airway epithelium provides the first line of defense to the surrounding environment. However, dysfunctions of this physical barrier are frequently observed in allergic diseases, which are tightly connected with pro- or anti-inflammatory processes. ... ...

    Abstract The airway epithelium provides the first line of defense to the surrounding environment. However, dysfunctions of this physical barrier are frequently observed in allergic diseases, which are tightly connected with pro- or anti-inflammatory processes. When the epithelial cells are confronted with allergens or pathogens, specific response mechanisms are set in motion, which in homeostasis, lead to the elimination of the invaders and leave permanent traces on the respiratory epithelium. However, allergens can also cause damage in the sensitized organism, which can be ascribed to the excessive immune reactions. The tight interaction of epithelial cells of the upper and lower airways with local and systemic immune cells can leave an imprint that may mirror the pathophysiology. The interaction with effector T cells, along with the macrophages, play an important role in this response, as reflected in the gene expression profiles (transcriptomes) of the epithelial cells, as well as in the secretory pattern (secretomes). Further, the storage of information from past exposures as memories within discrete cell types may allow a tissue to inform and fundamentally alter its future responses. Recently, several lines of evidence have highlighted the contributions from myeloid cells, lymphoid cells, stromal cells, mast cells, and epithelial cells to the emerging concepts of inflammatory memory and trained immunity.
    MeSH term(s) Allergens ; Epithelial Cells/metabolism ; Epithelium ; Humans ; Hypersensitivity ; Respiratory Mucosa
    Chemical Substances Allergens
    Language English
    Publishing date 2022-04-20
    Publishing country Switzerland
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells11091387
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  7. Article ; Online: Molecular determination of insect venom allergies.

    Blank, Simon / Jakwerth, Constanze A / Zissler, Ulrich M / Schmidt-Weber, Carsten B

    Expert review of molecular diagnostics

    2022  , Page(s) 1–4

    Language English
    Publishing date 2022-11-29
    Publishing country England
    Document type Editorial
    ZDB-ID 2112530-2
    ISSN 1744-8352 ; 1473-7159
    ISSN (online) 1744-8352
    ISSN 1473-7159
    DOI 10.1080/14737159.2022.2153038
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6073: Show issues Location:
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  8. Article ; Online: Flow-Based CL-SMIA for the Quantification of Protein Biomarkers from Nasal Secretions in Comparison with Sandwich ELISA.

    Neumair, Julia / Kröger, Marie / Stütz, Evamaria / Jerin, Claudia / Chaker, Adam M / Schmidt-Weber, Carsten B / Seidel, Michael

    Biosensors

    2023  Volume 13, Issue 7

    Abstract: Protein biomarkers in nasal secretions can be used as a measure to differentiate between allergies, airway diseases and infections for non-invasive diagnostics. The point-of-care quantification of biomarker levels using flow-based microarray facilitates ... ...

    Abstract Protein biomarkers in nasal secretions can be used as a measure to differentiate between allergies, airway diseases and infections for non-invasive diagnostics. The point-of-care quantification of biomarker levels using flow-based microarray facilitates precise and rapid diagnosis and displays the potential for targeted and effective treatment. For the first time, we developed a flow-based chemiluminescence sandwich microarray immunoassay (CL-SMIA) for the quantification of nasal interferon-beta (IFN-β) on the Microarray Chip Reader-Research (MCR-R). Polycarbonate foils are used as a cost-effective surface for immobilizing capture antibodies. By using a commercially available set of anti-human IFN-β antibodies, the CL-SMIA can be compared directly to an enzyme-linked immunosorbent assay (ELISA) performed in microtiter plates concerning the bioanalytical performance and economic issues. Pre-incubation of the sample with detection antibodies facilitates the lower consumption of detection antibodies, as this allows for a longer interaction time between the antibody and the biomarker. The direct injection of pre-incubated samples into the microarray chips eliminates the adsorption of proteins in the tubing as well as the contamination of the tubing and valves of the MCR-R with clinical samples. The small flow cell allows for a low sample volume of 50 μL. The limit of detection of 4.53 pg mL
    MeSH term(s) Immunoassay ; Enzyme-Linked Immunosorbent Assay ; Biomarkers/analysis ; Antibodies ; Oligonucleotide Array Sequence Analysis
    Chemical Substances Biomarkers ; Antibodies
    Language English
    Publishing date 2023-06-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662125-3
    ISSN 2079-6374 ; 2079-6374
    ISSN (online) 2079-6374
    ISSN 2079-6374
    DOI 10.3390/bios13070670
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  9. Article ; Online: Inhibition of SARS-CoV-2 infection and replication by Petasites hybridus CO2-extract (Ze 339).

    Jakwerth, Constanze A / Grass, Vincent / Erb, Anna / Pichlmair, Andreas / Boonen, Georg / Butterweck, Veronika / Schmidt-Weber, Carsten B

    Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

    2023  Volume 170, Page(s) 115959

    Abstract: Background: The intensified search for low-threshold herbal anti-viral drugs would be of great advantage in prevention of early stages of infection. Since the SARS-CoV-2 Omicron variant has prevailed in western countries, the course has only been mild, ... ...

    Abstract Background: The intensified search for low-threshold herbal anti-viral drugs would be of great advantage in prevention of early stages of infection. Since the SARS-CoV-2 Omicron variant has prevailed in western countries, the course has only been mild, but there are still no widely available drugs that can alleviate or shorten disease progression and counteract the development of Long-COVID. This study aimed to investigate the antiviral effects of a CO
    Methods: We analyzed the infection and replication rate of SARS-CoV-2 in primary normal human bronchial epithelial cells (NHBEs) using a GFP-expressing version of the wild-type SARS-CoV-2 virus and live cell imaging. Upon infection with a clinical isolate of the Omicron variant, viral RNA content was quantified, and plaque assays were performed. In addition, the human transcriptome was analyzed after 4- and 24-hours post infection using whole genome microarrays.
    Results: Ze 339 had a protective effect on primary airway epithelial cells during SARS-CoV-2 infection and impeded SARS-CoV-2 infection and replication in NHBE. Notably, Ze 339 inhibited the expression of infection-induced IFNA10 by 8.6-fold (p < 0.05) and additionally reduced a wide range of other interferons (IFNA6, IFNA7, IFNA8, IFNA21, IFNE, IFNW1).
    Conclusion: Thereby, Ze 339 attenuated epithelial infection by SARS-CoV-2 and modeled the IFN response. In conclusion, this study highlights Ze 339 as a potential treatment option for COVID-19 that limits infection-associated cell intrinsic immune responses.
    MeSH term(s) Humans ; COVID-19 ; SARS-CoV-2 ; Petasites ; Carbon Dioxide ; Post-Acute COVID-19 Syndrome ; Virus Replication
    Chemical Substances Ze 339 ; Carbon Dioxide (142M471B3J)
    Language English
    Publishing date 2023-12-07
    Publishing country France
    Document type Journal Article
    ZDB-ID 392415-4
    ISSN 1950-6007 ; 0753-3322 ; 0300-0893
    ISSN (online) 1950-6007
    ISSN 0753-3322 ; 0300-0893
    DOI 10.1016/j.biopha.2023.115959
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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.198: Show issues Location:
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  10. Article ; Online: Supporting allergen-specific immunotherapy by inhibition of Janus kinases.

    Gutermuth, Jan / Schmidt-Weber, Carsten B / Blank, Simon

    Allergy

    2019  Volume 74, Issue 9, Page(s) 1814–1816

    MeSH term(s) Allergens/administration & dosage ; Allergens/immunology ; Animals ; Desensitization, Immunologic/methods ; Humans ; Hypersensitivity/etiology ; Hypersensitivity/metabolism ; Hypersensitivity/therapy ; Janus Kinase Inhibitors/pharmacology ; Janus Kinase Inhibitors/therapeutic use ; Janus Kinases/antagonists & inhibitors ; Mice
    Chemical Substances Allergens ; Janus Kinase Inhibitors ; Janus Kinases (EC 2.7.10.2)
    Language English
    Publishing date 2019-05-20
    Publishing country Denmark
    Document type News
    ZDB-ID 391933-x
    ISSN 1398-9995 ; 0105-4538
    ISSN (online) 1398-9995
    ISSN 0105-4538
    DOI 10.1111/all.13808
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