Article ; Online: Methods to Investigate Signal Transduction Pathways in Trypanosoma cruzi: Cyclic Nucleotide Phosphodiesterases Assay Protocols.
Methods in molecular biology (Clifton, N.J.)
2020 Volume 2116, Page(s) 523–534
Abstract: Intracellular levels of cyclic nucleotide second messengers are regulated predominantly by a large superfamily of phosphodiesterases (PDEs). Most of the different PDE variants play specific physiological functions; in fact, PDEs can associate with other ... ...
Abstract | Intracellular levels of cyclic nucleotide second messengers are regulated predominantly by a large superfamily of phosphodiesterases (PDEs). Most of the different PDE variants play specific physiological functions; in fact, PDEs can associate with other proteins allowing them to be strategically anchored throughout the cell. In this regard, precise cellular expression and compartmentalization of these enzymes produce the specific control of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) gradients in cells and enable their integration with other signaling pathways.In trypanosomatids, some PDEs are essential for their survival and play fundamental roles in the adaptation of these parasites to different environmental stresses, as well as in the differentiation between their different life cycle forms. Given that these enzymes not only are similar to human PDEs but also have differential biochemical properties, and due to the great knowledge of drugs that target human PDEs, trypanosomatid PDEs could be postulated as important therapeutic targets through the repositioning of drugs.In this chapter, we describe a simple and sensitive radioisotope-based method to measure cyclic 3',5'-nucleotide phosphodiesterase using [ |
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MeSH term(s) | 3',5'-Cyclic-AMP Phosphodiesterases/chemistry ; 3',5'-Cyclic-AMP Phosphodiesterases/isolation & purification ; 3',5'-Cyclic-AMP Phosphodiesterases/metabolism ; Cyclic AMP/chemistry ; Cyclic AMP/metabolism ; Enzyme Assays/methods ; Isotope Labeling/methods ; Life Cycle Stages ; Protozoan Proteins/chemistry ; Protozoan Proteins/isolation & purification ; Protozoan Proteins/metabolism ; Signal Transduction ; Tritium/chemistry ; Trypanosoma cruzi/metabolism |
Chemical Substances | Protozoan Proteins ; Tritium (10028-17-8) ; Cyclic AMP (E0399OZS9N) ; 3',5'-Cyclic-AMP Phosphodiesterases (EC 3.1.4.17) |
Language | English |
Publishing date | 2020-03-27 |
Publishing country | United States |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ISSN | 1940-6029 |
ISSN (online) | 1940-6029 |
DOI | 10.1007/978-1-0716-0294-2_31 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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