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  1. Book ; Thesis: Die Regulation der 4-Dihydromethyltrisporat-Dehydrogenase während der sexuellen und parasitischen Interaktion von Parasitella parasitica

    Schultze, Kornelia

    2005  

    Author's details von Kornelia Schultze
    Language German
    Size 98 Bl., Ill., graph. Darst., 30 cm
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Univ., Diss.--Jena, 2005
    Note Auch als elektronisches Dokument vorhanden
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  2. Book ; Online ; Thesis: Die Regulation der 4-Dihydromethyltrisporat-Dehydrogenase während der sexuellen und parasitischen Interaktion von Parasitella parasitica

    Schultze, Kornelia [Verfasser]

    2005  

    Author's details von Kornelia Schultze
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  3. Article: Sexuality and parasitism share common regulatory pathways in the fungus Parasitella parasitica.

    Schultze, Kornelia / Schimek, Christine / Wöstemeyer, Johannes / Burmester, Anke

    Gene

    2005  Volume 348, Page(s) 33–44

    Abstract: Parasitella parasitica, a facultative mycoparasite of zygomycetous fungi, forms cytoplasmic fusions with its hosts during infection. Thus, the organism is an efficient donor of genetic material in parasexual host-parasite interactions. Recognition ... ...

    Abstract Parasitella parasitica, a facultative mycoparasite of zygomycetous fungi, forms cytoplasmic fusions with its hosts during infection. Thus, the organism is an efficient donor of genetic material in parasexual host-parasite interactions. Recognition between parasite and host is mediated by trisporoids, which are also responsible for sexual communication. The TDH gene for one of the key enzymes of trisporic acid biosynthesis, 4-dihydromethyl-trisporate dehydrogenase, was cloned and its transcription analysed. TDH was cloned on a 6175-bp insert and was found to map in a complex cluster of genes that suggest post-transcriptional antisense regulation. Histochemical TDH analysis in developing parasitic or sexual structures shows high enzymatic activity in Parasitella. TDH is linked to a gene for a putative acyl-CoA thioesterase (ACT). Two ORFs were identified in the 5'-region of the TDH gene, a third one, coding for 176 amino acids overlaps the ACT gene in antisense direction completely. Expression levels of ACT and ORF1 depend on parasitic and sexual interactions.
    MeSH term(s) Amino Acid Sequence ; Base Sequence ; Blotting, Northern ; Cloning, Molecular ; DNA, Fungal/chemistry ; DNA, Fungal/genetics ; Fatty Acids, Unsaturated/biosynthesis ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Gene Expression Regulation, Fungal ; Genes, Fungal/genetics ; Genetic Linkage ; Molecular Sequence Data ; Mucorales/genetics ; Mucorales/growth & development ; Mucorales/metabolism ; Open Reading Frames/genetics ; Oxidoreductases/genetics ; Oxidoreductases/metabolism ; Palmitoyl-CoA Hydrolase/genetics ; Poly A/genetics ; RNA, Fungal/genetics ; RNA, Fungal/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Sequence Homology, Nucleic Acid
    Chemical Substances DNA, Fungal ; Fatty Acids, Unsaturated ; Fungal Proteins ; RNA, Fungal ; trisporic acid (11043-91-7) ; Poly A (24937-83-5) ; Oxidoreductases (EC 1.-) ; dihydromethyl-trisporate dehydrogenase (EC 1.-) ; Palmitoyl-CoA Hydrolase (EC 3.1.2.2)
    Language English
    Publishing date 2005-03-28
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 391792-7
    ISSN 1879-0038 ; 0378-1119
    ISSN (online) 1879-0038
    ISSN 0378-1119
    DOI 10.1016/j.gene.2005.01.007
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1357: Show issues Location:
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  4. Article ; Online: Retrocyte Display® technology: generation and screening of a high diversity cellular antibody library.

    Breous-Nystrom, Ekaterina / Schultze, Kornelia / Meier, Marco / Flueck, Lukas / Holzer, Christina / Boll, Melanie / Seibert, Volker / Schuster, Andrea / Blanusa, Milan / Schaefer, Verena / Grawunder, Ulf / Martin-Parras, Luis / van Dijk, Marc A

    Methods (San Diego, Calif.)

    2014  Volume 65, Issue 1, Page(s) 57–67

    Abstract: Over the last nearly three decades in vitro display technologies have played an important role in the discovery and optimization of antibodies and other proteins for therapeutic applications. Here we describe the use of retroviral expression technology ... ...

    Abstract Over the last nearly three decades in vitro display technologies have played an important role in the discovery and optimization of antibodies and other proteins for therapeutic applications. Here we describe the use of retroviral expression technology for the display of full-length IgG on B lineage cells in vitro with a hallmark of a tight and stable genotype to phenotype coupling. We describe the creation of a high-diversity (>1.0E09 different heavy- and light-chain combinations) cell displayed fully human antibody library from healthy donor-derived heavy- and light-chain gene libraries, and demonstrate the recovery of high affinity target-specific antibodies from this library by staining of cells with a labeled target antigen and their magnetic- and flow cytometry-based cell sorting. The present technology represents a further evolution in the discovery of full-length, fully human antibodies using mammalian display, and is termed Retrocyte Display® (Retroviral B lymphocyte Display).
    MeSH term(s) Animals ; Antibodies, Monoclonal/biosynthesis ; Antibodies, Monoclonal/genetics ; B-Lymphocytes/metabolism ; Cryopreservation ; Drug Evaluation, Preclinical ; Flow Cytometry ; Genetic Variation ; Genetic Vectors ; HEK293 Cells ; Humans ; Immunoglobulin Heavy Chains/biosynthesis ; Immunoglobulin Heavy Chains/genetics ; Immunoglobulin Light Chains/biosynthesis ; Immunoglobulin Light Chains/genetics ; Immunomagnetic Separation ; Peptide Library ; Protein Binding ; Retroviridae/genetics
    Chemical Substances Antibodies, Monoclonal ; Immunoglobulin Heavy Chains ; Immunoglobulin Light Chains ; Peptide Library
    Language English
    Publishing date 2014-01-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1066584-5
    ISSN 1095-9130 ; 1046-2023
    ISSN (online) 1095-9130
    ISSN 1046-2023
    DOI 10.1016/j.ymeth.2013.09.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3239: Show issues Location:
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  5. Article: 4-Dihydromethyltrisporate dehydrogenase, an enzyme of the sex hormone pathway in Mucor mucedo, is constitutively transcribed but its activity is differently regulated in (+) and (-) mating types.

    Schimek, Christine / Petzold, Annett / Schultze, Kornelia / Wetzel, Jana / Wolschendorf, Frank / Burmester, Anke / Wöstemeyer, Johannes

    Fungal genetics and biology : FG & B

    2005  Volume 42, Issue 9, Page(s) 804–812

    Abstract: 4-Dihydromethyltrisporate dehydrogenase (TDH) converts the (+) mating type sex pheromone 4-dihydromethyltrisporate into methyltrisporate. In Mucor mucedo, this conversion is required only in the (-) mating type. Expression of the TDH encoding TSP1 gene ... ...

    Abstract 4-Dihydromethyltrisporate dehydrogenase (TDH) converts the (+) mating type sex pheromone 4-dihydromethyltrisporate into methyltrisporate. In Mucor mucedo, this conversion is required only in the (-) mating type. Expression of the TDH encoding TSP1 gene was analyzed qualitatively using reverse-transcribed PCR. TSP1 is constitutively transcribed in the (+) and in the (-) mating type, irrespective of the mating situation. By immunodetection, the translation product is also formed constitutively. In contrast to gene expression, TDH enzyme activity depends on the sexual status of the mycelium. Activity is restricted to the sexually stimulated (-) mating type. Non-stimulated (-), as well as stimulated and non-stimulated (+) mycelia exhibit no activity and do not influence activity in stimulated (-) mycelia. Time course analysis shows strongly increased enzyme activity at 80 min after stimulation. Low activity exists from the onset of stimulation, indicating that additional regulation mechanisms are involved in TDH function.
    MeSH term(s) Blotting, Western ; DNA, Fungal/chemistry ; DNA, Fungal/genetics ; Gene Expression Regulation, Fungal ; Molecular Sequence Data ; Mucor/enzymology ; Mucor/genetics ; Mycelium/enzymology ; Oxidoreductases/genetics ; Oxidoreductases/metabolism ; RNA, Fungal/analysis ; RNA, Messenger/analysis ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Analysis, DNA ; Transcription, Genetic
    Chemical Substances DNA, Fungal ; RNA, Fungal ; RNA, Messenger ; Oxidoreductases (EC 1.-) ; dihydromethyl-trisporate dehydrogenase (EC 1.-)
    Language English
    Publishing date 2005-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1319820-8
    ISSN 1096-0937 ; 1087-1845 ; 0147-5975
    ISSN (online) 1096-0937
    ISSN 1087-1845 ; 0147-5975
    DOI 10.1016/j.fgb.2005.05.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2431: Show issues Location:
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  6. Article: Cleavage of beta-carotene as the first step in sexual hormone synthesis in zygomycetes is mediated by a trisporic acid regulated beta-carotene oxygenase.

    Burmester, Anke / Richter, Mareike / Schultze, Kornelia / Voelz, Kerstin / Schachtschabel, Doreen / Boland, Wilhelm / Wöstemeyer, Johannes / Schimek, Christine

    Fungal genetics and biology : FG & B

    2007  Volume 44, Issue 11, Page(s) 1096–1108

    Abstract: Carotene cleavage is the necessary initial step in the biosynthesis of trisporic acid, the sexual signal in zygomycete fungi. Two genes encoding putative carotene oxygenases, designated tsp3 and tsp4, were identified in the genome of the zygomycete ... ...

    Abstract Carotene cleavage is the necessary initial step in the biosynthesis of trisporic acid, the sexual signal in zygomycete fungi. Two genes encoding putative carotene oxygenases, designated tsp3 and tsp4, were identified in the genome of the zygomycete Rhizopus oryzae. Using heterologous primers, tsp3 was cloned and sequenced also from Blakeslea trispora. tsp3 transcription correlates with sexual development in both species. Northern hybridization of B. trispora mRNA revealed strong induction of tsp3 transcription in mated cultures. A very strong and direct transient induction of transcription by trisporic acid was proven by quantitative real-time PCR analysis. In R. oryzae, transcriptional induction is also inducible by stimulation with trisporoids and depends on the developmental stage of the mycelium. The functionality of the tsp3 gene product as carotene cleavage enzyme was shown as loss of carotene in an Escherichia coli strain transformed to carotene production and tsp3 expression.
    MeSH term(s) Fatty Acids, Unsaturated ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Fungi ; Gene Expression Regulation, Fungal ; Oxygenases/metabolism ; Polymerase Chain Reaction ; RNA, Messenger/biosynthesis ; Transcription, Genetic ; beta Carotene/biosynthesis ; beta Carotene/chemistry ; beta Carotene/metabolism
    Chemical Substances Fatty Acids, Unsaturated ; Fungal Proteins ; RNA, Messenger ; beta Carotene (01YAE03M7J) ; trisporic acid (11043-91-7) ; Oxygenases (EC 1.13.-) ; beta-carotene ketolase (EC 1.13.-)
    Language English
    Publishing date 2007-11
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1319820-8
    ISSN 1096-0937 ; 1087-1845 ; 0147-5975
    ISSN (online) 1096-0937
    ISSN 1087-1845 ; 0147-5975
    DOI 10.1016/j.fgb.2007.07.008
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2431: Show issues Location:
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