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  1. Article ; Online: Live imaging of the Drosophila ovarian germline stem cell niche

    Scott G. Wilcockson / Hilary L. Ashe

    STAR Protocols, Vol 2, Iss 1, Pp 100371- (2021)

    2021  

    Abstract: Summary: The maintenance of stem cell populations and the differentiation of their progeny is coordinated by specific communication with associated niche cells. Here, we describe a protocol for short-term live imaging of the Drosophila ovarian germline ... ...

    Abstract Summary: The maintenance of stem cell populations and the differentiation of their progeny is coordinated by specific communication with associated niche cells. Here, we describe a protocol for short-term live imaging of the Drosophila ovarian germline stem cell niche ex vivo. By immobilizing the ovarian tissue in a fibrinogen-thrombin clot, we are able to maintain the tissue for short-term high-temporal live imaging. This enables the visualization of dynamic cellular processes, such as the cytoskeletal dynamics that control stem cell niche communication.For complete details on the use and execution of this protocol, please refer to Wilcockson and Ashe (2019).
    Keywords Cell Biology ; Microscopy ; Model Organisms ; Stem Cells ; Science (General) ; Q1-390
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Peak BMP Responses in the Drosophila Embryo Are Dependent on the Activation of Integrin Signaling

    Annick Sawala / Margherita Scarcia / Catherine Sutcliffe / Scott G. Wilcockson / Hilary L. Ashe

    Cell Reports, Vol 12, Iss 10, Pp 1584-

    2015  Volume 1593

    Abstract: Within a 3D tissue, cells need to integrate signals from growth factors, such as BMPs, and the extracellular matrix (ECM) to coordinate growth and differentiation. Here, we use the Drosophila embryo as a model to investigate how BMP responses are ... ...

    Abstract Within a 3D tissue, cells need to integrate signals from growth factors, such as BMPs, and the extracellular matrix (ECM) to coordinate growth and differentiation. Here, we use the Drosophila embryo as a model to investigate how BMP responses are influenced by a cell’s local ECM environment. We show that integrins, which are ECM receptors, are absolutely required for peak BMP signaling. This stimulatory effect of integrins requires their intracellular signaling function, which is activated by the ECM protein collagen IV. Mechanistically, integrins interact with the BMP receptor and stimulate phosphorylation of the downstream Mad transcription factor. The BMP-pathway-enhancing function of integrins is independent of focal adhesion kinase, but it requires conserved NPXY motifs in the β-integrin cytoplasmic tail. Furthermore, we show that an α-integrin subunit is a BMP target gene, identifying positive feedback between integrin signaling and BMP pathway activity that may contribute to robust cell fate decisions.
    Keywords Biology (General) ; QH301-705.5
    Subject code 571
    Language English
    Publishing date 2015-09-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: Peak BMP Responses in the Drosophila Embryo Are Dependent on the Activation of Integrin Signaling

    Annick Sawala / Margherita Scarcia / Catherine Sutcliffe / Scott G. Wilcockson / Hilary L. Ashe

    Cell Reports, Vol 13, Iss 7, Pp 1519-

    2015  Volume 1520

    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2015-11-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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