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  1. Article ; Online: Quantitative mechanical stimulation of GPR68 using a novel 96 well flow plugin.

    Segeritz, Philipp / Kolesnik, Kirill / Scott, Daniel J / Collins, David J

    Lab on a chip

    2024  Volume 24, Issue 6, Page(s) 1616–1625

    Abstract: Mechanosensitive proteins play a crucial role in a range of physiological processes, including hearing, tactile sensation and regulating blood flow. While previous work has demonstrated the mechanosensitivity of several proteins, the ability to apply ... ...

    Abstract Mechanosensitive proteins play a crucial role in a range of physiological processes, including hearing, tactile sensation and regulating blood flow. While previous work has demonstrated the mechanosensitivity of several proteins, the ability to apply precisely defined mechanical forces to cells in a consistent, replicable manner remains a significant challenge. In this work we present a novel 96-well plate-compatible plugin device for generating highly-controlled flow-based mechanical simulation of cells, which enables quantitative assessment of mechanosensitive protein function. The device is used to mechanically stimulate HEK 293T cells expressing the mechanosensitive protein GPR68, a G protein-coupled receptor. By assaying intracellular calcium levels during flow-based cell stimulation, we determine that GPR68 signalling is a function of the applied shear-force. As this approach is compatible with conventional cell culture plates and allows for simultaneous readout in a conventional fluorescence plate reader, this represents a valuable new tool to investigate mechanotransduction.
    MeSH term(s) Mechanotransduction, Cellular/physiology ; Cell Culture Techniques ; Receptors, G-Protein-Coupled/metabolism ; Stress, Mechanical
    Chemical Substances Receptors, G-Protein-Coupled
    Language English
    Publishing date 2024-03-12
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2056646-3
    ISSN 1473-0189 ; 1473-0197
    ISSN (online) 1473-0189
    ISSN 1473-0197
    DOI 10.1039/d3lc00767g
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Sub-wavelength acoustic stencil for tailored micropatterning.

    Kolesnik, Kirill / Segeritz, Philipp / Scott, Daniel J / Rajagopal, Vijay / Collins, David J

    Lab on a chip

    2023  Volume 23, Issue 10, Page(s) 2447–2457

    Abstract: Acoustofluidic devices are ideal for biomedical micromanipulation applications, with high biocompatibility and the ability to generate force gradients down to the scale of cells. However, complex and designed patterning at the microscale remains ... ...

    Abstract Acoustofluidic devices are ideal for biomedical micromanipulation applications, with high biocompatibility and the ability to generate force gradients down to the scale of cells. However, complex and designed patterning at the microscale remains challenging. In this work we report an acoustofluidic approach to direct particles and cells within a structured surface in arbitrary configurations. Wells, trenches and cavities are embedded in this surface. Combined with a half-wavelength acoustic field, together these form an 'acoustic stencil' where arbitrary cell and particle arrangements can be reversibly generated. Here a bulk-wavemode lithium niobate resonator generates multiplexed parallel patterning
    Language English
    Publishing date 2023-05-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 2056646-3
    ISSN 1473-0189 ; 1473-0197
    ISSN (online) 1473-0189
    ISSN 1473-0197
    DOI 10.1039/d3lc00043e
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Respiration mask waveguide optimisation for maximised speech intelligibility.

    Kolesnik, Kirill / Bryan, David / Harley, William / Segeritz, Philipp / Guest, Matthew / Rajagopal, Vijay / Collins, David J

    The Journal of the Acoustical Society of America

    2021  Volume 150, Issue 3, Page(s) 2030

    Abstract: Both the scarcity and environmental impact of disposable face masks, as in the COVID-19 pandemic, have instigated the recent development of reusable masks. Such face masks reduce transmission of infectious agents and particulates, but often impact a user' ...

    Abstract Both the scarcity and environmental impact of disposable face masks, as in the COVID-19 pandemic, have instigated the recent development of reusable masks. Such face masks reduce transmission of infectious agents and particulates, but often impact a user's ability to be understood when materials, such as silicone or hard polymers, are used. In this work, we present a numerical optimisation approach to optimise waveguide topology, where a waveguide is used to transmit and direct sound from the interior of the mask volume to the outside air. This approach allows acoustic energy to be maximised according to specific frequency bands, including those most relevant to human speech. We employ this method to convert a resuscitator mask, made of silicone, into respiration personal protective equipment (PPE) that maximises the speech intelligibility index (SII). We validate this approach experimentally as well, showing improved SII when using the fabricated device. Together, this design represents a unique and effective approach to utilize and adapt available apparatus to filter air while improving the ability to communicate effectively, including in healthcare settings.
    MeSH term(s) COVID-19 ; Humans ; Masks ; Pandemics ; Respiration ; SARS-CoV-2 ; Speech Intelligibility
    Language English
    Publishing date 2021-10-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 219231-7
    ISSN 1520-8524 ; 0001-4966
    ISSN (online) 1520-8524
    ISSN 0001-4966
    DOI 10.1121/10.0006235
    Database MEDical Literature Analysis and Retrieval System OnLINE

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