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  1. Article ; Online: Recognition of polymorphic Csd proteins determines sex in the honeybee.

    Otte, Marianne / Netschitailo, Oksana / Weidtkamp-Peters, Stefanie / Seidel, Claus A M / Beye, Martin

    Science advances

    2023  Volume 9, Issue 40, Page(s) eadg4239

    Abstract: Sex in honeybees, ...

    Abstract Sex in honeybees,
    MeSH term(s) Bees/genetics ; Female ; Male ; Animals ; Amino Acid Sequence ; Sex Determination Processes/genetics ; Genotype ; Polymorphism, Genetic ; Heterozygote
    Language English
    Publishing date 2023-10-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2810933-8
    ISSN 2375-2548 ; 2375-2548
    ISSN (online) 2375-2548
    ISSN 2375-2548
    DOI 10.1126/sciadv.adg4239
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: An optofluidic antenna for enhancing the sensitivity of single-emitter measurements.

    Morales-Inostroza, Luis / Folz, Julian / Kühnemuth, Ralf / Felekyan, Suren / Wieser, Franz-Ferdinand / Seidel, Claus A M / Götzinger, Stephan / Sandoghdar, Vahid

    Nature communications

    2024  Volume 15, Issue 1, Page(s) 2545

    Abstract: Many single-molecule investigations are performed in fluidic environments, for example, to avoid unwanted consequences of contact with surfaces. Diffusion of molecules in this arrangement limits the observation time and the number of collected photons, ... ...

    Abstract Many single-molecule investigations are performed in fluidic environments, for example, to avoid unwanted consequences of contact with surfaces. Diffusion of molecules in this arrangement limits the observation time and the number of collected photons, thus, compromising studies of processes with fast or slow dynamics. Here, we introduce a planar optofluidic antenna (OFA), which enhances the fluorescence signal from molecules by about 5 times per passage, leads to about 7-fold more frequent returns to the observation volume, and significantly lengthens the diffusion time within one passage. We use single-molecule multi-parameter fluorescence detection (sm-MFD), fluorescence correlation spectroscopy (FCS) and Förster resonance energy transfer (FRET) measurements to characterize our OFAs. The antenna advantages are showcased by examining both the slow (ms) and fast (50 μs) dynamics of DNA four-way (Holliday) junctions with real-time resolution. The FRET trajectories provide evidence for the absence of an intermediate conformational state and introduce an upper bound for its lifetime. The ease of implementation and compatibility with various microscopy modalities make OFAs broadly applicable to a diverse range of studies.
    Language English
    Publishing date 2024-03-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-024-46730-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Book ; Online ; Thesis: Protein Structure and Dynamics probed by Multiparameter Fluorescence

    Folz, Julian [Verfasser] / Seidel, Claus A. M. [Gutachter] / Herrmann, Christian [Gutachter]

    2023  

    Author's details Julian Folz ; Gutachter: Claus A. M. Seidel, Christian Herrmann
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language English
    Publisher Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf
    Publishing place Düsseldorf
    Document type Book ; Online ; Thesis
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  4. Book ; Online ; Thesis: Towards improved FRET-derived biophysical models of proteins

    Popara, Milana [Verfasser] / Seidel, Claus A. M. [Gutachter] / Gohlke, Holger [Gutachter]

    2023  

    Author's details Milana Popara ; Gutachter: Claus A. M. Seidel, Holger Gohlke
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language English
    Publisher Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf
    Publishing place Düsseldorf
    Document type Book ; Online ; Thesis
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  5. Article: Fundamental photophysics of isomorphic and expanded fluorescent nucleoside analogues

    Dziuba, Dmytro / Didier, Pascal / Ciaco, Stefano / Barth, Anders / Seidel, Claus A. M. / Mély, Yves

    Chemical Society reviews. 2021 June 21, v. 50, no. 12

    2021  

    Abstract: Fluorescent nucleoside analogues (FNAs) are structurally diverse mimics of the natural essentially non-fluorescent nucleosides which have found numerous applications in probing the structure and dynamics of nucleic acids as well as their interactions ... ...

    Abstract Fluorescent nucleoside analogues (FNAs) are structurally diverse mimics of the natural essentially non-fluorescent nucleosides which have found numerous applications in probing the structure and dynamics of nucleic acids as well as their interactions with various biomolecules. In order to minimize disturbance in the labelled nucleic acid sequences, the FNA chromophoric groups should resemble the natural nucleobases in size and hydrogen-bonding patterns. Isomorphic and expanded FNAs are the two groups that best meet the criteria of non-perturbing fluorescent labels for DNA and RNA. Significant progress has been made over the past decades in understanding the fundamental photophysics that governs the spectroscopic and environmentally sensitive properties of these FNAs. Herein, we review recent advances in the spectroscopic and computational studies of selected isomorphic and expanded FNAs. We also show how this information can be used as a rational basis to design new FNAs, select appropriate sequences for optimal spectroscopic response and interpret fluorescence data in FNA applications.
    Keywords DNA ; RNA ; fluorescence ; hydrogen bonding ; nucleobases ; nucleosides ; spectroscopy
    Language English
    Dates of publication 2021-0621
    Size p. 7062-7107.
    Publishing place The Royal Society of Chemistry
    Document type Article
    ZDB-ID 1472875-8
    ISSN 1460-4744 ; 0306-0012
    ISSN (online) 1460-4744
    ISSN 0306-0012
    DOI 10.1039/d1cs00194a
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: PARP1-DNA co-condensation drives DNA repair site assembly to prevent disjunction of broken DNA ends.

    Chappidi, Nagaraja / Quail, Thomas / Doll, Simon / Vogel, Laura T / Aleksandrov, Radoslav / Felekyan, Suren / Kühnemuth, Ralf / Stoynov, Stoyno / Seidel, Claus A M / Brugués, Jan / Jahnel, Marcus / Franzmann, Titus M / Alberti, Simon

    Cell

    2024  Volume 187, Issue 4, Page(s) 945–961.e18

    Abstract: DNA double-strand breaks (DSBs) are repaired at DSB sites. How DSB sites assemble and how broken DNA is prevented from separating is not understood. Here we uncover that the synapsis of broken DNA is mediated by the DSB sensor protein poly(ADP-ribose) ( ... ...

    Abstract DNA double-strand breaks (DSBs) are repaired at DSB sites. How DSB sites assemble and how broken DNA is prevented from separating is not understood. Here we uncover that the synapsis of broken DNA is mediated by the DSB sensor protein poly(ADP-ribose) (PAR) polymerase 1 (PARP1). Using bottom-up biochemistry, we reconstitute functional DSB sites and show that DSB sites form through co-condensation of PARP1 multimers with DNA. The co-condensates exert mechanical forces to keep DNA ends together and become enzymatically active for PAR synthesis. PARylation promotes release of PARP1 from DNA ends and the recruitment of effectors, such as Fused in Sarcoma, which stabilizes broken DNA ends against separation, revealing a finely orchestrated order of events that primes broken DNA for repair. We provide a comprehensive model for the hierarchical assembly of DSB condensates to explain DNA end synapsis and the recruitment of effector proteins for DNA damage repair.
    MeSH term(s) DNA/metabolism ; DNA Breaks, Double-Stranded ; DNA Damage ; DNA Repair ; Poly (ADP-Ribose) Polymerase-1/genetics ; Poly (ADP-Ribose) Polymerase-1/metabolism ; Humans
    Chemical Substances DNA (9007-49-2) ; Poly (ADP-Ribose) Polymerase-1 (EC 2.4.2.30) ; PARP1 protein, human (EC 2.4.2.30)
    Language English
    Publishing date 2024-02-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 187009-9
    ISSN 1097-4172 ; 0092-8674
    ISSN (online) 1097-4172
    ISSN 0092-8674
    DOI 10.1016/j.cell.2024.01.015
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1167: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 58: Show issues

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  7. Book: Ultra-empfindliche und ultra-schnelle Forschungs- und Technikkamera für neue Anwendungen mit breitem Einsatz in Lebenswissenschaften und Umweltüberwachung

    Seidel, Claus A. M

    exemplarische Evaluierung der Leistungsfähigkeit in den Bereichen Neurobiologie, Zellkernforschung und Photosynthese ; Teilprojekt: Evaluierung der Leistungsfähigkeit der ultraempfindlichen und ultraschnellen Forschungs- und Technikkamera ; Teilprojekt-Abschlussbericht nach 48 Projektmonaten (zum 31.12.2012) ; Laufzeit 01.01.09 - 31.12.12 ; [Abschlussbericht 2012 zum Projekt "Quantum"]

    2012  

    Title variant Forschungskamera
    Institution Universität Düsseldorf
    Author's details Heinrich-Heine-Universität Düsseldorf. Claus A. M. Seidel
    Language German
    Size 16 Bl., Ill., graph. Darst.
    Publishing place Düsseldorf
    Document type Book
    Note Förderkennzeichen BMBF 13N10075. - Verbund-Nr. 01066853 ; Unterschiede zwischen dem gedruckten Dokument und der elektronischen Ressource können nicht ausgeschlossen werden
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  8. Book ; Online: Ultra-empfindliche und ultra-schnelle Forschungs- und Technikkamera für neue Anwendungen mit breitem Einsatz in Lebenswissenschaften und Umweltüberwachung

    Seidel, Claus A. M

    exemplarische Evaluierung der Leistungsfähigkeit in den Bereichen Neurobiologie, Zellkernforschung und Photosynthese ; Teilprojekt: Evaluierung der Leistungsfähigkeit der ultraempfindlichen und ultraschnellen Forschungs- und Technikkamera ; Teilprojekt-Abschlussbericht nach 48 Projektmonaten (zum 31.12.2012) ; Laufzeit 01.01.09 - 31.12.12 ; Abschlussbericht 2012 zum Projekt "Quantum"

    2012  

    Title variant Forschungskamera
    Institution Universität Düsseldorf
    Author's details Heinrich-Heine-Universität Düsseldorf. Claus A. M. Seidel
    Language German
    Size Online-Ressource (16 S., 1,53 MB), Ill., graph. Darst.
    Publisher Technische Informationsbibliothek u. Universitätsbibliothek
    Publishing place Hannover ; Düsseldorf
    Document type Book ; Online
    Note Förderkennzeichen BMBF 13N10075. - Verbund-Nr. 01066853 ; Unterschiede zwischen dem gedruckten Dokument und der elektronischen Ressource können nicht ausgeschlossen werden
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  9. Article ; Online: Fundamental photophysics of isomorphic and expanded fluorescent nucleoside analogues.

    Dziuba, Dmytro / Didier, Pascal / Ciaco, Stefano / Barth, Anders / Seidel, Claus A M / Mély, Yves

    Chemical Society reviews

    2021  Volume 50, Issue 12, Page(s) 7062–7107

    Abstract: Fluorescent nucleoside analogues (FNAs) are structurally diverse mimics of the natural essentially non-fluorescent nucleosides which have found numerous applications in probing the structure and dynamics of nucleic acids as well as their interactions ... ...

    Abstract Fluorescent nucleoside analogues (FNAs) are structurally diverse mimics of the natural essentially non-fluorescent nucleosides which have found numerous applications in probing the structure and dynamics of nucleic acids as well as their interactions with various biomolecules. In order to minimize disturbance in the labelled nucleic acid sequences, the FNA chromophoric groups should resemble the natural nucleobases in size and hydrogen-bonding patterns. Isomorphic and expanded FNAs are the two groups that best meet the criteria of non-perturbing fluorescent labels for DNA and RNA. Significant progress has been made over the past decades in understanding the fundamental photophysics that governs the spectroscopic and environmentally sensitive properties of these FNAs. Herein, we review recent advances in the spectroscopic and computational studies of selected isomorphic and expanded FNAs. We also show how this information can be used as a rational basis to design new FNAs, select appropriate sequences for optimal spectroscopic response and interpret fluorescence data in FNA applications.
    MeSH term(s) Fluorescence ; Fluorescent Dyes/chemistry ; Nucleosides/chemistry ; Photochemical Processes
    Chemical Substances Fluorescent Dyes ; Nucleosides
    Language English
    Publishing date 2021-05-06
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1472875-8
    ISSN 1460-4744 ; 0306-0012
    ISSN (online) 1460-4744
    ISSN 0306-0012
    DOI 10.1039/d1cs00194a
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Unraveling multi-state molecular dynamics in single-molecule FRET experiments. I. Theory of FRET-lines.

    Barth, Anders / Opanasyuk, Oleg / Peulen, Thomas-Otavio / Felekyan, Suren / Kalinin, Stanislav / Sanabria, Hugo / Seidel, Claus A M

    The Journal of chemical physics

    2022  Volume 156, Issue 14, Page(s) 141501

    Abstract: Conformational dynamics of biomolecules are of fundamental importance for their function. Single-molecule studies of Förster Resonance Energy Transfer (smFRET) between a tethered donor and acceptor dye pair are a powerful tool to investigate the ... ...

    Abstract Conformational dynamics of biomolecules are of fundamental importance for their function. Single-molecule studies of Förster Resonance Energy Transfer (smFRET) between a tethered donor and acceptor dye pair are a powerful tool to investigate the structure and dynamics of labeled molecules. However, capturing and quantifying conformational dynamics in intensity-based smFRET experiments remains challenging when the dynamics occur on the sub-millisecond timescale. The method of multiparameter fluorescence detection addresses this challenge by simultaneously registering fluorescence intensities and lifetimes of the donor and acceptor. Together, two FRET observables, the donor fluorescence lifetime τ
    MeSH term(s) Fluorescence Resonance Energy Transfer/methods ; Molecular Conformation ; Molecular Dynamics Simulation
    Language English
    Publishing date 2022-04-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3113-6
    ISSN 1089-7690 ; 0021-9606
    ISSN (online) 1089-7690
    ISSN 0021-9606
    DOI 10.1063/5.0089134
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 4' 49/16: Show issues

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