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  1. Article ; Online: Presence and activity of nitrogen-fixing bacteria in Scots pine needles in a boreal forest: a nitrogen-addition experiment.

    Bizjak, Tinkara / Sellstedt, Anita / Gratz, Regina / Nordin, Annika

    Tree physiology

    2023  Volume 43, Issue 8, Page(s) 1354–1364

    Abstract: Endophytic nitrogen-fixing bacteria have been detected and isolated from the needles of conifer trees growing in North American boreal forests. Because boreal forests are nutrient-limited, these bacteria could provide an important source of nitrogen for ... ...

    Abstract Endophytic nitrogen-fixing bacteria have been detected and isolated from the needles of conifer trees growing in North American boreal forests. Because boreal forests are nutrient-limited, these bacteria could provide an important source of nitrogen for tree species. This study aimed to determine their presence and activity in a Scandinavian boreal forest, using immunodetection of nitrogenase enzyme subunits and acetylene-reduction assays of native Scots pine (Pinus sylvestris L.) needles. The presence and rate of nitrogen fixation by endophytic bacteria were compared between control plots and fertilized plots in a nitrogen-addition experiment. In contrast to the expectation that nitrogen-fixation rates would decline in fertilized plots, as seen, for instance, with nitrogen-fixing bacteria associated with bryophytes, there was no difference in the presence or activity of nitrogen-fixing bacteria between the two treatments. The extrapolated calculated rate of nitrogen fixation relevant for the forest stand was 20 g N ha-1 year-1, which is rather low compared with Scots pine annual nitrogen use but could be important for the nitrogen-poor forest in the long term. In addition, of 13 colonies of potential nitrogen-fixing bacteria isolated from the needles on nitrogen-free media, 10 showed in vitro nitrogen fixation. In summary, 16S rRNA sequencing identified the species as belonging to the genera Bacillus, Variovorax, Novosphingobium, Sphingomonas, Microbacterium and Priestia, which was confirmed by Illumina whole-genome sequencing. Our results confirm the presence of endophytic nitrogen-fixing bacteria in Scots pine needles and suggest that they could be important for the long-term nitrogen budget of the Scandinavian boreal forest.
    MeSH term(s) Nitrogen-Fixing Bacteria ; Taiga ; Pinus sylvestris ; RNA, Ribosomal, 16S ; Acetylene ; Nitrogen
    Chemical Substances RNA, Ribosomal, 16S ; Acetylene (OC7TV75O83) ; Nitrogen (N762921K75)
    Language English
    Publishing date 2023-04-18
    Publishing country Canada
    Document type Journal Article
    ZDB-ID 743341-4
    ISSN 1758-4469 ; 0829-318X
    ISSN (online) 1758-4469
    ISSN 0829-318X
    DOI 10.1093/treephys/tpad048
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 4737: Show issues

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  2. Article ; Online: Metabolomic Study of Heterotrophically Grown

    Nzayisenga, Jean Claude / Sellstedt, Anita

    Molecules (Basel, Switzerland)

    2021  Volume 26, Issue 9

    Abstract: There are numerous strains ... ...

    Abstract There are numerous strains of
    MeSH term(s) Biomass ; Chlorella/growth & development ; Chlorella/metabolism ; Fatty Acids/metabolism ; Heterotrophic Processes ; Metabolome ; Metabolomics/methods ; Microalgae/metabolism ; Sweden ; Waste Water
    Chemical Substances Fatty Acids ; Waste Water
    Language English
    Publishing date 2021-04-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules26092410
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.MO 81: Show issues

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  3. Article: Effects of light intensity on growth and lipid production in microalgae grown in wastewater.

    Nzayisenga, Jean Claude / Farge, Xavier / Groll, Sophia Leticia / Sellstedt, Anita

    Biotechnology for biofuels

    2020  Volume 13, Page(s) 4

    Abstract: Background: Cultivation of microalgae in wastewater could significantly contribute to wastewater treatment, biodiesel production, and thus the transition to renewable energy. However, more information on effects of environmental factors, including light ...

    Abstract Background: Cultivation of microalgae in wastewater could significantly contribute to wastewater treatment, biodiesel production, and thus the transition to renewable energy. However, more information on effects of environmental factors, including light intensity, on their growth and composition (particularly fatty acid contents) is required. Therefore, we investigated the biomass and fatty acid production of four microalgal species, isolated in the Northern hemisphere and grown at three light intensities (50, 150 and 300 μE m
    Results: Increases in light intensities resulted in higher biomass of all four species and, importantly, raised fatty acid contents of both
    Conclusion: The results show the importance of optimizing light intensities to improve fatty acid production by microalgae and their quality as sources of biodiesel. In addition, increase in fatty acid content is associated with decrease in protein content.
    Language English
    Publishing date 2020-01-07
    Publishing country England
    Document type Journal Article
    ZDB-ID 2421351-2
    ISSN 1754-6834
    ISSN 1754-6834
    DOI 10.1186/s13068-019-1646-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Mixotrophic and heterotrophic production of lipids and carbohydrates by a locally isolated microalga using wastewater as a growth medium.

    Nzayisenga, Jean Claude / Eriksson, Karolina / Sellstedt, Anita

    Bioresource technology

    2018  Volume 257, Page(s) 260–265

    Abstract: The biomass production and changes in biochemical composition of a locally isolated microalga (Chlorella sp.) were investigated in autotrophic, mixotrophic and heterotrophic conditions, using glucose or glycerol as carbon sources and municipal wastewater ...

    Abstract The biomass production and changes in biochemical composition of a locally isolated microalga (Chlorella sp.) were investigated in autotrophic, mixotrophic and heterotrophic conditions, using glucose or glycerol as carbon sources and municipal wastewater as the growth medium. Both standard methods and Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) analysis of data acquired by Fourier-transform IR (FTIR) spectrometry showed that autotrophic and mixotrophic conditions promoted carbohydrate accumulation, while heterotrophic conditions with glycerol resulted in the highest lipid content and lowest carbohydrate content. Heterotrophic conditions with glycerol as a carbon source also resulted in high oleic acid (18:1) contents and low linolenic acid (18:3) contents, and thus increasing biodiesel quality. The results also show the utility of MCR-ALS for analyzing changes in microalgal biochemical composition.
    MeSH term(s) Biofuels ; Biomass ; Carbohydrates ; Chlorella ; Heterotrophic Processes ; Lipids ; Microalgae ; Waste Water
    Chemical Substances Biofuels ; Carbohydrates ; Lipids ; Waste Water
    Language English
    Publishing date 2018-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 1065195-0
    ISSN 1873-2976 ; 0960-8524
    ISSN (online) 1873-2976
    ISSN 0960-8524
    DOI 10.1016/j.biortech.2018.02.085
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 3258: Show issues

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  5. Book ; Conference proceedings: Special issue: Frankia and Actionorhizal plants

    Sellstedt, Anita

    [The 14th International Meeting on Frankia and Actinorhizal Plants took place in Ume°a, Sweden from 15 July 2006 to 18 July 2006]

    (Physiologia plantaruem ; 130.2007,3)

    2007  

    Event/congress International Meeting on Frankia and Actinorhizal Plants (14, 2006.07.15-17, Ume°a)
    Author's details [ed. Anita Sellstedt ...]
    Series title Physiologia plantaruem ; 130.2007,3
    Language English
    Size S. 315 - 470, Ill., graph. Darst
    Publisher Blackwell
    Publishing place Oxford
    Document type Book ; Conference proceedings
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  6. Article: Effects of light intensity on growth and lipid production in microalgae grown in wastewater

    Nzayisenga, Jean Claude / Farge, Xavier / Groll, Sophia Leticia / Sellstedt, Anita

    Biotechnology for biofuels. 2020 Dec., v. 13, no. 1

    2020  

    Abstract: BACKGROUND: Cultivation of microalgae in wastewater could significantly contribute to wastewater treatment, biodiesel production, and thus the transition to renewable energy. However, more information on effects of environmental factors, including light ... ...

    Abstract BACKGROUND: Cultivation of microalgae in wastewater could significantly contribute to wastewater treatment, biodiesel production, and thus the transition to renewable energy. However, more information on effects of environmental factors, including light intensity, on their growth and composition (particularly fatty acid contents) is required. Therefore, we investigated the biomass and fatty acid production of four microalgal species, isolated in the Northern hemisphere and grown at three light intensities (50, 150 and 300 μE m⁻² s⁻¹). RESULTS: Increases in light intensities resulted in higher biomass of all four species and, importantly, raised fatty acid contents of both Desmodesmus sp. and Scenedesmus obliquus. Fourier-transform IR spectrometry analysis showed that the increases in fatty acid content were associated with reductions in protein, but not carbohydrate, contents. Assessment of fatty acid composition revealed that increasing light intensity led to higher and lower contents of oleic (18:1) and linolenic (18:3) acids, respectively. The microalgae consumed more than 75% of the nitrogen and phosphorus present in the wastewater used as growth medium. CONCLUSION: The results show the importance of optimizing light intensities to improve fatty acid production by microalgae and their quality as sources of biodiesel. In addition, increase in fatty acid content is associated with decrease in protein content.
    Keywords Desmodesmus ; Scenedesmus obliquus ; biodiesel ; biomass ; biotechnology ; carbohydrates ; culture media ; environmental factors ; fatty acid composition ; fatty acids ; information ; light intensity ; microalgae ; nitrogen ; phosphorus ; protein content ; spectroscopy ; wastewater ; wastewater treatment
    Language English
    Dates of publication 2020-12
    Size p. 4.
    Publishing place BioMed Central
    Document type Article
    Note NAL-light
    ZDB-ID 2421351-2
    ISSN 1754-6834
    ISSN 1754-6834
    DOI 10.1186/s13068-019-1646-x
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: More than protection: the function of TiO

    Kawde, Anurag / Annamalai, Alagappan / Sellstedt, Anita / Uhlig, Jens / Wågberg, Thomas / Glatzel, Pieter / Messinger, Johannes

    Physical chemistry chemical physics : PCCP

    2020  Volume 22, Issue 48, Page(s) 28459–28467

    Abstract: Worldwide significant efforts are ongoing to develop devices that store solar energy as fuels. In one such approach, solar energy is absorbed by semiconductors and utilized directly by catalysts at their surfaces to split water into H2 and O2. To protect ...

    Abstract Worldwide significant efforts are ongoing to develop devices that store solar energy as fuels. In one such approach, solar energy is absorbed by semiconductors and utilized directly by catalysts at their surfaces to split water into H2 and O2. To protect the semiconductors in these photo-electrochemical cells (PEC) from corrosion, frequently thin TiO2 interlayers are applied. Employing a well-performing photoanode comprised of 1-D n-Si microwires (MWs) covered with a mesoporous (mp) TiO2 interlayer fabricated by solution processing and functionalized with α-Fe2O3 nanorods, we studied here the function of this TiO2 interlayer by high-energy resolution fluorescence detected X-ray absorption near edge structure (HERFD-XANES) spectroscopy, along with X-ray emission spectroscopy (XES) and standard characterization techniques. Our data reveal that the TiO2 interlayer not only protects the n-Si MW surface from corrosion, but that it also acts as a template for the hydrothermal growth of α-Fe2O3 nanorods and improves the photocatalytic efficiency. We show that the latter effect correlates with the presence of stable oxygen vacancies at the interface between mp-TiO2 and α-Fe2O3, which act as electron traps and thereby substantially reduce the charge recombination rate at the hematite surface.
    Language English
    Publishing date 2020-12-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 1476244-4
    ISSN 1463-9084 ; 1463-9076
    ISSN (online) 1463-9084
    ISSN 1463-9076
    DOI 10.1039/d0cp04280c
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Aspects of nitrogen-fixing Actinobacteria, in particular free-living and symbiotic Frankia.

    Sellstedt, Anita / Richau, Kerstin H

    FEMS microbiology letters

    2013  Volume 342, Issue 2, Page(s) 179–186

    Abstract: Studies of nitrogen-fixing properties among the Gram-positive Actinobacteria revealed that some species of Arthrobacter, Agromyces, Corynebacterium, Mycobacterium, Micromonospora, Propionibacteria and Streptomyces have nitrogen-fixing capacity. This is ... ...

    Abstract Studies of nitrogen-fixing properties among the Gram-positive Actinobacteria revealed that some species of Arthrobacter, Agromyces, Corynebacterium, Mycobacterium, Micromonospora, Propionibacteria and Streptomyces have nitrogen-fixing capacity. This is also valid for Frankia that fix nitrogen both in free-living and in symbiotic conditions. Frankia symbiosis results from interaction between the Frankia bacteria and dicotyledonous plants, that is, actinorhiza. These plants, which are important in forestry and agroforestry, form, together with the legumes (Fabales), a single nitrogen-fixing clade. It has been shown that a receptor-like kinase gene, SymRK, is necessary for nodulation in actinorhizal plants as well as in legumes and arbuscular mycorrhizal fungi. Recently, the involvement of isoflavonoids as signal molecules during nodulation of an actinorhizal plant was shown. The genome sizes of three Frankia species, Frankia EANpec, ACN14a and CcI3, are different, revealing a relationship between genome size and geographical distribution. Recent genomic sequencing data of Frankia represent genomes from cluster I to IV, indicating that the genome of DgI is one of the smallest genomes in Frankia. In addition, nonsymbiotic Frankiales such as Acidothermus cellulolyticus, Blastococcus saxoobsidens, Geodermatophilus obscurus and Modestobacter marinus have a variety of genome sizes ranging from 2.4 to 5.57 Mb.
    MeSH term(s) Frankia/classification ; Frankia/growth & development ; Frankia/physiology ; Genetic Variation ; Genome, Bacterial ; Nitrogen Fixation ; Phylogeography ; Plants/microbiology ; Symbiosis
    Language English
    Publishing date 2013-05
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 752343-9
    ISSN 1574-6968 ; 0378-1097
    ISSN (online) 1574-6968
    ISSN 0378-1097
    DOI 10.1111/1574-6968.12116
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1372: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Article ; Online: Screening Suitability of Northern Hemisphere Algal Strains for Heterotrophic Cultivation and Fatty Acid Methyl Ester Production.

    Nzayisenga, Jean Claude / Niemi, Calle / Ferro, Lorenza / Gorzsas, Andras / Gentili, Francesco G / Funk, Christiane / Sellstedt, Anita

    Molecules (Basel, Switzerland)

    2020  Volume 25, Issue 9

    Abstract: Rapid rises in atmospheric ... ...

    Abstract Rapid rises in atmospheric CO
    MeSH term(s) Autotrophic Processes ; Biomass ; Esters/metabolism ; Fatty Acids/metabolism ; Heterotrophic Processes ; Lipids/biosynthesis ; Methylation ; Microalgae/growth & development ; Microalgae/metabolism ; Spectroscopy, Fourier Transform Infrared
    Chemical Substances Esters ; Fatty Acids ; Lipids
    Language English
    Publishing date 2020-04-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules25092107
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    Zs.MO 81: Show issues

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  10. Article ; Online: A microstructured p-Si photocathode outcompetes Pt as a counter electrode to hematite in photoelectrochemical water splitting.

    Kawde, Anurag / Annamalai, Alagappan / Sellstedt, Anita / Glatzel, Pieter / Wågberg, Thomas / Messinger, Johannes

    Dalton transactions (Cambridge, England : 2003)

    2018  Volume 48, Issue 4, Page(s) 1166–1170

    Abstract: Herein, we communicate about an Earth-abundant semiconductor photocathode (p-Si/TiO2/NiOx) as an alternative for the rare and expensive Pt as a counter electrode for overall photoelectrochemical water splitting. The proposed photoelectrochemical (PEC) ... ...

    Abstract Herein, we communicate about an Earth-abundant semiconductor photocathode (p-Si/TiO2/NiOx) as an alternative for the rare and expensive Pt as a counter electrode for overall photoelectrochemical water splitting. The proposed photoelectrochemical (PEC) water-splitting device mimics the "Z"-scheme observed in natural photosynthesis by combining two photoelectrodes in a parallel-illumination mode. A nearly 60% increase in the photocurrent density (Jph) for pristine α-Fe2O3 and a 77% increase in the applied bias photocurrent efficiency (ABPE) were achieved by replacing the conventionally used Pt cathode with an efficient, cost effective p-Si/TiO2/NiOx photocathode under parallel illumination. The resulting photocurrent density of 1.26 mA cm-2 at 1.23VRHE represents a new record performance for hydrothermally grown pristine α-Fe2O3 nanorod photoanodes in combination with a photocathode, which opens the prospect for further improvement by doping α-Fe2O3 or by its decoration with co-catalysts. Electrochemical impedance spectroscopy measurements suggest that this significant performance increase is due to the enhancement of the space-charge field in α-Fe2O3.
    Language English
    Publishing date 2018-11-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 1472887-4
    ISSN 1477-9234 ; 1364-5447 ; 0300-9246 ; 1477-9226
    ISSN (online) 1477-9234 ; 1364-5447
    ISSN 0300-9246 ; 1477-9226
    DOI 10.1039/c8dt03653e
    Database MEDical Literature Analysis and Retrieval System OnLINE

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