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Artikel ; Online: Linear and Nonlinear Elastic Properties of Polystyrene-Based Nanocomposites with Allotropic Carbon Fillers and Binary Mixtures.

Belashov, Andrey V / Zhikhoreva, Anna A / Moskalyuk, Olga A / Beltukov, Yaroslav M / Semenova, Irina V

2022 Band 14, Heft 24

Abstract: We report measurements of linear and nonlinear elastic properties of polystyrene-based nanocomposites with six types of nanofillers, including single and binary mixtures of allotropic carbon nanoparticles. Composite samples were fabricated by the same ... ...

Abstract	We report measurements of linear and nonlinear elastic properties of polystyrene-based nanocomposites with six types of nanofillers, including single and binary mixtures of allotropic carbon nanoparticles. Composite samples were fabricated by the same technology and contained the same filler concentration (5% wt.), which allowed for a direct comparison of their properties. It was shown that the most significant variations of linear and nonlinear elastic properties occur in different nanocomposites. In particular, the most pronounced enhancements of linear elastic moduli (in about 50%) obtained in tensile and flexural tests and in dynamic mechanical analysis were recorded in the sample filled with spherical fullerene nanoparticles. While the most profound rise of absolute values of nonlinear elastic moduli (tens of times) was obtained in the sample filled with the mixture of carbon nanotubes and graphene. The observed tendencies demonstrated the synergistic effect of fillers of different dimensionality on the elastic properties of nanocomposites.
Sprache	Englisch
Erscheinungsdatum	2022-12-13
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article
ZDB-ID	2527146-5
ISSN	2073-4360 ; 2073-4360
ISSN (online)	2073-4360
ISSN	2073-4360
DOI	10.3390/polym14245462
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: The B-box1 domain of PML mediates SUMO E2-E3 complex formation through an atypical interaction with UBC9.

Bregnard, Thomas / Ahmed, Affrin / Semenova, Irina V / Weller, Sandra K / Bezsonova, Irina

Biophysical chemistry

2022 Band 287, Seite(n) 106827

Abstract: The small, ubiquitin-like modifier SUMO is covalently attached to substrates by the enzyme UBC9. SUMO conjugation of substrates often requires an E3 ligase, which ensures substrate specificity by simultaneously binding UBC9 and the substrate. E3 SUMO ... ...

Abstract	The small, ubiquitin-like modifier SUMO is covalently attached to substrates by the enzyme UBC9. SUMO conjugation of substrates often requires an E3 ligase, which ensures substrate specificity by simultaneously binding UBC9 and the substrate. E3 SUMO ligases commonly use a RING domain to engage UBC9. The Promyelocytic Leukemia protein (PML) has been implicated as a probable SUMO ligase. Although PML does contain a RING domain, which is expected to recruit UBC9, we demonstrate that PML RING does not bind UBC9 in vitro. Instead, we show that isolated PML B-box1 possesses UBC9-binding activity and map the B-box1 binding site on UBC9. This site also binds the upstream E1 enzyme that transfers SUMO to UBC9. The overlap of these two binding sites suggests that UBC9 cannot interact with its E1 and E3 partners simultaneously. Furthermore, we present a model of the PML dimer that supports the accessibility of B-box1 for UBC9 binding in the context of the full-length PML.
Mesh-Begriff(e)	Binding Sites ; Substrate Specificity ; Transcription Factors/metabolism ; Ubiquitin/chemistry ; Ubiquitin-Conjugating Enzymes/chemistry ; Ubiquitin-Conjugating Enzymes/metabolism
Chemische Substanzen	Transcription Factors ; Ubiquitin ; Ubiquitin-Conjugating Enzymes (EC 2.3.2.23)
Sprache	Englisch
Erscheinungsdatum	2022-05-18
Erscheinungsland	Netherlands
Dokumenttyp	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural
ZDB-ID	185052-0
ISSN	1873-4200 ; 0301-4622
ISSN (online)	1873-4200
ISSN	0301-4622
DOI	10.1016/j.bpc.2022.106827
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Conformational exchange at a C

Bregnard, Thomas A / Fairchild, Daniel / Erlandsen, Heidi / Semenova, Irina V / Szczepaniak, Renata / Ahmed, Affrin / Weller, Sandra K / Korzhnev, Dmitry M / Bezsonova, Irina

Structure (London, England : 1993)

2023 Band 31, Heft 9, Seite(n) 1086–1099.e6

Abstract: The promyelocytic leukemia protein, PML, plays a vital role in the cellular response to oxidative stress; however, the molecular mechanism of its action remains poorly understood. Here, we identify redox-sensitive sites of PML. A molecule of PML is ... ...

Abstract	The promyelocytic leukemia protein, PML, plays a vital role in the cellular response to oxidative stress; however, the molecular mechanism of its action remains poorly understood. Here, we identify redox-sensitive sites of PML. A molecule of PML is cysteine-rich and contains three zinc-binding domains including RING, B-box1, and B-box2. Using in vitro assays, we have compared the sensitivity of the isolated RING and B-box1 domains and shown that B-box1 is more sensitive to oxidation. NMR studies of PML dynamics showed that one of the Zn-coordination sites within the B-box1 undergoes significant conformational exchange, revealing a hotspot for exposure of reactive cysteines. In agreement with the in vitro data, enhancement of the B-box1 Zn-coordination dynamics led to more efficient recruitment of PML into PML nuclear bodies in cells. Overall, our results suggest that the increased sensitivity of B-box1 to oxidative stress makes this domain an important redox-sensing component of PML.
Mesh-Begriff(e)	Nuclear Proteins/metabolism ; Zinc/metabolism ; Promyelocytic Leukemia Protein/genetics ; Promyelocytic Leukemia Protein/metabolism ; Binding Sites ; Oxidation-Reduction
Chemische Substanzen	Nuclear Proteins ; Zinc (J41CSQ7QDS) ; Promyelocytic Leukemia Protein
Sprache	Englisch
Erscheinungsdatum	2023-07-19
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	1213087-4
ISSN	1878-4186 ; 0969-2126
ISSN (online)	1878-4186
ISSN	0969-2126
DOI	10.1016/j.str.2023.06.014
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: A Novel Interaction Between RAD23A/B and Y-family DNA Polymerases.

Ashton, Nicholas W / Jaiswal, Nancy / Moreno, Natália Cestari / Semenova, Irina V / D'Orlando, Dana A / Latancia, Marcela Teatin / McIntyre, Justyna / Woodgate, Roger / Bezsonova, Irina

Journal of molecular biology

2023 Band 435, Heft 24, Seite(n) 168353

Abstract: The Y-family DNA polymerases - Pol ι, Pol η, Pol κ and Rev1 - are most well-known for their roles in the DNA damage tolerance pathway of translesion synthesis (TLS). They function to overcome replication barriers by bypassing DNA damage lesions that ... ...

Abstract	The Y-family DNA polymerases - Pol ι, Pol η, Pol κ and Rev1 - are most well-known for their roles in the DNA damage tolerance pathway of translesion synthesis (TLS). They function to overcome replication barriers by bypassing DNA damage lesions that cannot be normally replicated, allowing replication forks to continue without stalling. In this work, we demonstrate a novel interaction between each Y-family polymerase and the nucleotide excision repair (NER) proteins, RAD23A and RAD23B. We initially focus on the interaction between RAD23A and Pol ι, and through a series of biochemical, cell-based, and structural assays, find that the RAD23A ubiquitin-binding domains (UBA1 and UBA2) interact with separate sites within the Pol ι catalytic domain. While this interaction involves the ubiquitin-binding cleft of UBA2, Pol ι interacts with a distinct surface on UBA1. We further find that mutating or deleting either UBA domain disrupts the RAD23A-Pol ι interaction, demonstrating that both interactions are necessary for stable binding. We also provide evidence that both RAD23 proteins interact with Pol ι in a similar manner, as well as with each of the Y-family polymerases. These results shed light on the interplay between the different functions of the RAD23 proteins and reveal novel binding partners for the Y-family TLS polymerases.
Mesh-Begriff(e)	DNA Damage ; DNA Polymerase iota/chemistry ; DNA Repair ; DNA Replication ; DNA-Directed DNA Polymerase/metabolism ; Ubiquitins/chemistry ; DNA-Binding Proteins/chemistry ; DNA Repair Enzymes/chemistry
Chemische Substanzen	DNA Polymerase iota (EC 2.7.7.7) ; DNA-Directed DNA Polymerase (EC 2.7.7.7) ; Ubiquitins ; RAD23A protein, human (156533-33-4) ; RAD23B protein, human ; DNA-Binding Proteins ; DNA Repair Enzymes (EC 6.5.1.-)
Sprache	Englisch
Erscheinungsdatum	2023-11-05
Erscheinungsland	Netherlands
Dokumenttyp	Journal Article ; Research Support, N.I.H., Intramural ; Research Support, N.I.H., Extramural
ZDB-ID	80229-3
ISSN	1089-8638 ; 0022-2836
ISSN (online)	1089-8638
ISSN	0022-2836
DOI	10.1016/j.jmb.2023.168353
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Application of digital holographic tomography in antitumor effect of cantharides complex on 4T1 breast cancer cells.

Lu, Chen-Wen / Belashov, Andrey V / Zhikhoreva, Anna A / Semenova, Irina V / Cheng, Chau-Jern / Su, Li-Yu / Wu, Chung-Hsin

Applied optics

2021 Band 60, Heft 12, Seite(n) 3365–3373

Abstract: The study focuses on a methodology providing noninvasive monitoring and evaluation of the antitumor effect of traditional Chinese medicine, cantharides complex (canth), on 4T1 breast tumor cells. Digital holographic tomography (DHT) and developed data ... ...

Abstract	The study focuses on a methodology providing noninvasive monitoring and evaluation of the antitumor effect of traditional Chinese medicine, cantharides complex (canth), on 4T1 breast tumor cells. Digital holographic tomography (DHT) and developed data post-processing algorithms were used for quantitative estimation of changes in optical and morphological parameters of cells. We calculated and compared data on the refractive index, thickness, and projected area of 4T1 breast tumor cells in control untreated specimens and those treated with doxorubicin hydrochloride (DOX), canth, and their combinations. Post-treatment changes in cellular morphology recorded by DHT demonstrated that the two drugs led to noticeably different morphological changes in cells that can be presumably associated with different pathways of their death, apoptosis, or necrosis. The effect of combined treatment with these two drugs strongly depended on their relative concentrations and could lead to changes characteristic either for DOX or for canth; however, being more profound than those obtained when using each drug solely. The results obtained by DHT are in a good correspondence with commonly used cell viability analysis and immunofluorescent analysis of changes in cellular cytoskeleton.
Mesh-Begriff(e)	Animals ; Antineoplastic Agents/pharmacology ; Antineoplastic Combined Chemotherapy Protocols/pharmacology ; Breast Neoplasms/diagnostic imaging ; Breast Neoplasms/drug therapy ; Breast Neoplasms/pathology ; Cantharidin/pharmacology ; Cell Line, Tumor ; Cell Survival/drug effects ; Doxorubicin/pharmacology ; Female ; Fluorescent Antibody Technique ; Holography/methods ; Medicine, Chinese Traditional ; Mice ; Refractometry/methods ; Tomography/methods
Chemische Substanzen	Antineoplastic Agents ; Doxorubicin (80168379AG) ; Cantharidin (IGL471WQ8P)
Sprache	Englisch
Erscheinungsdatum	2021-05-13
Erscheinungsland	United States
Dokumenttyp	Journal Article
ISSN	1539-4522
ISSN (online)	1539-4522
DOI	10.1364/AO.416943
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Machine Learning Assisted Classification of Cell Lines and Cell States on Quantitative Phase Images.

Belashov, Andrey V / Zhikhoreva, Anna A / Belyaeva, Tatiana N / Salova, Anna V / Kornilova, Elena S / Semenova, Irina V / Vasyutinskii, Oleg S

Cells

2021 Band 10, Heft 10

Abstract: In this report, we present implementation and validation of machine-learning classifiers for distinguishing between cell types (HeLa, A549, 3T3 cell lines) and states (live, necrosis, apoptosis) based on the analysis of optical parameters derived from ... ...

Abstract	In this report, we present implementation and validation of machine-learning classifiers for distinguishing between cell types (HeLa, A549, 3T3 cell lines) and states (live, necrosis, apoptosis) based on the analysis of optical parameters derived from cell phase images. Validation of the developed classifier shows the accuracy for distinguishing between the three cell types of about 93% and between different cell states of the same cell line of about 89%. In the field test of the developed algorithm, we demonstrate successful evaluation of the temporal dynamics of relative amounts of live, apoptotic and necrotic cells after photodynamic treatment at different doses.
Mesh-Begriff(e)	Cell Line, Tumor/classification ; HeLa Cells/metabolism ; Humans ; Machine Learning/standards ; Microscopy, Phase-Contrast/methods
Sprache	Englisch
Erscheinungsdatum	2021-09-29
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2661518-6
ISSN	2073-4409 ; 2073-4409
ISSN (online)	2073-4409
ISSN	2073-4409
DOI	10.3390/cells10102587
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Relaxation dynamics of alkyl derivatives of fluorescein MitoFluo and C

Beltukova, Dina M / Belik, Victor P / Semak, Bogdan V / Semenova, Irina V / Smolin, Andrey G / Vasyutinskii, Oleg S

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

2021 Band 263, Seite(n) 120145

Abstract: We present results of experimental and theoretical studies of excited state dynamics in two alkyl derivatives of fluorescein, MitoFluo and ... ...

Abstract	We present results of experimental and theoretical studies of excited state dynamics in two alkyl derivatives of fluorescein, MitoFluo and C
Mesh-Begriff(e)	Diffusion ; Fluorescein ; Fluorescence Polarization ; Liposomes ; Spectrometry, Fluorescence
Chemische Substanzen	Liposomes ; Fluorescein (TPY09G7XIR)
Sprache	Englisch
Erscheinungsdatum	2021-07-05
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	210413-1
ISSN	1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
ISSN (online)	1873-3557
ISSN	0370-8322 ; 0584-8539 ; 1386-1425
DOI	10.1016/j.saa.2021.120145
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Polystyrene-Based Nanocomposites with Different Fillers: Fabrication and Mechanical Properties.

Moskalyuk, Olga A / Belashov, Andrey V / Beltukov, Yaroslav M / Ivan'kova, Elena M / Popova, Elena N / Semenova, Irina V / Yelokhovsky, Vladimir Y / Yudin, Vladimir E

Polymers

2020 Band 12, Heft 11

Abstract: The paper presents a comprehensive analysis of the elastic properties of polystyrene-based nanocomposites filled with different types of inclusions: small spherical particles ( ... ...

Abstract	The paper presents a comprehensive analysis of the elastic properties of polystyrene-based nanocomposites filled with different types of inclusions: small spherical particles (SiO
Sprache	Englisch
Erscheinungsdatum	2020-10-23
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article
ZDB-ID	2527146-5
ISSN	2073-4360 ; 2073-4360
ISSN (online)	2073-4360
ISSN	2073-4360
DOI	10.3390/polym12112457
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Protein kinase A, which regulates intracellular transport, forms complexes with molecular motors on organelles.

Kashina, Anna S / Semenova, Irina V / Ivanov, Pavel A / Potekhina, Ekaterina S / Zaliapin, Ilya / Rodionov, Vladimir I

Current biology : CB

2004 Band 14, Heft 20, Seite(n) 1877–1881

Abstract: Major signaling cascades have been shown to play a role in the regulation of intracellular organelle transport . Aggregation and dispersion of pigment granules in melanophores are regulated by the second messenger cAMP through the protein kinase A (PKA) ... ...

Abstract	Major signaling cascades have been shown to play a role in the regulation of intracellular organelle transport . Aggregation and dispersion of pigment granules in melanophores are regulated by the second messenger cAMP through the protein kinase A (PKA) signaling pathway however, the exact mechanisms of this regulation are poorly understood. To study the role of signaling molecules in the regulation of pigment transport in melanophores, we have asked the question whether the components of the cAMP-signaling pathway are bound to pigment granules and whether they interact with molecular motors to regulate the granule movement throughout the cytoplasm. We found that purified pigment granules contain PKA and scaffolding proteins and that PKA associates with pigment granules in cells. Furthermore, we found that the PKA regulatory subunit forms two separate complexes, one with cytoplasmic dynein ("aggregation complex") and one with kinesin II and myosin V ("dispersion complex"), and that the removal of PKA from granules causes dissociation of dynein and disruption of dynein-dependent pigment aggregation. We conclude that cytoplasmic organelles contain protein complexes that include motor proteins and signaling molecules involved in different components of intracellular transport. We propose to call such complexes 'regulated motor units' (RMU).
Mesh-Begriff(e)	Animals ; Biological Transport/physiology ; Calcium-Binding Proteins/metabolism ; Cell Line ; Cyclic AMP-Dependent Protein Kinases/metabolism ; Dyneins/metabolism ; Immunoblotting ; Immunoprecipitation ; Kinesin ; Melanophores/metabolism ; Microinjections ; Molecular Motor Proteins/metabolism ; Muscle Proteins/metabolism ; Myosin Type V/metabolism ; Organelles/physiology ; Pigments, Biological/metabolism ; Signal Transduction/physiology ; Transfection ; Xenopus ; Xenopus Proteins
Chemische Substanzen	Calcium-Binding Proteins ; Molecular Motor Proteins ; Muscle Proteins ; Pigments, Biological ; XKLP3 protein, Xenopus ; Xenopus Proteins ; kinesin-II (147096-75-1) ; Cyclic AMP-Dependent Protein Kinases (EC 2.7.11.11) ; Myosin Type V (EC 3.6.1.-) ; Dyneins (EC 3.6.4.2) ; Kinesin (EC 3.6.4.4)
Sprache	Englisch
Erscheinungsdatum	2004-10-26
Erscheinungsland	England
Dokumenttyp	Comparative Study ; Journal Article ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	1071731-6
ISSN	1879-0445 ; 0960-9822
ISSN (online)	1879-0445
ISSN	0960-9822
DOI	10.1016/j.cub.2004.10.003
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Formation of a new structure of landed property and land tenure as a part of agrarian reform

Krylatych, Ėlʹmira N / Semenova, Irina V / Uzun, V. Ja

Studies on Russian economic development : the official journal of the Institute of Economic Forecasting, Russian Academy of Sciences 7 ,4, S. 314-321

materials of socioeconomic monitoring

1996

Verfasserangabe	E. N. Krylatykh, I. V. Semenova, and V. Ya. Uzun
Schlagwörter	Agrarreform ; Wirkungsanalyse ; Russland
Sprache	Englisch
Verlag	Pleiades Publ
Erscheinungsort	Moscow
Dokumenttyp	Artikel
ZDB-ID	913675-7
ISSN	1054-6588
Datenquelle	ECONomics Information System

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