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  1. Article ; Online: Lighting the way: how the

    Septer, Alecia N / Visick, Karen L

    Journal of bacteriology

    2024  , Page(s) e0003524

    Abstract: ... ...

    Abstract Vibrio
    Language English
    Publishing date 2024-05-02
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2968-3
    ISSN 1098-5530 ; 0021-9193
    ISSN (online) 1098-5530
    ISSN 0021-9193
    DOI 10.1128/jb.00035-24
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.50: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  2. Article: The

    Septer, Alecia N

    mSystems

    2019  Volume 4, Issue 3

    Abstract: The symbiosis ... ...

    Abstract The symbiosis between
    Language English
    Publishing date 2019-06-11
    Publishing country United States
    Document type Journal Article
    ISSN 2379-5077
    ISSN 2379-5077
    DOI 10.1128/mSystems.00108-19
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: The

    Septer, Alecia N / Sharpe, Garrett / Shook, Erika A

    bioRxiv : the preprint server for biology

    2023  

    Abstract: The type VI secretion system (T6SS) is an interbacterial weapon composed of thousands of protein subunits and predicted to require significant cellular energy to deploy, yet a fitness cost from T6SS use is rarely observed. Here, we identify host-like ... ...

    Abstract The type VI secretion system (T6SS) is an interbacterial weapon composed of thousands of protein subunits and predicted to require significant cellular energy to deploy, yet a fitness cost from T6SS use is rarely observed. Here, we identify host-like conditions where the T6SS incurs a fitness cost using the beneficial symbiont,
    Language English
    Publishing date 2023-03-07
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.03.07.529561
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Quantification of Interbacterial Competition using Single-Cell Fluorescence Imaging.

    Smith, Stephanie / Septer, Alecia N

    Journal of visualized experiments : JoVE

    2021  , Issue 175

    Abstract: Interbacterial competition can directly impact the structure and function of microbiomes. This work describes a fluorescence microscopy approach that can be used to visualize and quantify competitive interactions between different bacterial strains at ... ...

    Abstract Interbacterial competition can directly impact the structure and function of microbiomes. This work describes a fluorescence microscopy approach that can be used to visualize and quantify competitive interactions between different bacterial strains at the single-cell level. The protocol described here provides methods for advanced approaches in slide preparation on both upright and inverted epifluorescence microscopes, live-cell and time-lapse imaging techniques, and quantitative image analysis using the open-source software FIJI. The approach in this manuscript outlines the quantification of competitive interactions between symbiotic Vibrio fischeri populations by measuring the change in area over time for two coincubated strains that are expressing different fluorescent proteins from stable plasmids. Alternative methods are described for optimizing this protocol in bacterial model systems that require different growth conditions. Although the assay described here uses conditions optimized for V. fischeri, this approach is highly reproducible and can easily be adapted to study competition among culturable isolates from diverse microbiomes.
    MeSH term(s) Aliivibrio fischeri ; Microscopy, Fluorescence ; Optical Imaging ; Plasmids ; Symbiosis
    Language English
    Publishing date 2021-09-02
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Video-Audio Media
    ZDB-ID 2259946-0
    ISSN 1940-087X ; 1940-087X
    ISSN (online) 1940-087X
    ISSN 1940-087X
    DOI 10.3791/62851
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Quantification of interbacterial competition using single-cell fluorescence imaging

    Smith, Stephanie / Septer, Alecia N.

    Journal of visualized experiments. 2021 Sept. 02, , no. 175

    2021  

    Abstract: Interbacterial competition can directly impact the structure and function of microbiomes. This work describes a fluorescence microscopy approach that can be used to visualize and quantify competitive interactions between different bacterial strains at ... ...

    Abstract Interbacterial competition can directly impact the structure and function of microbiomes. This work describes a fluorescence microscopy approach that can be used to visualize and quantify competitive interactions between different bacterial strains at the single-cell level. The protocol described here provides methods for advanced approaches in slide preparation on both upright and inverted epifluorescence microscopes, live-cell and time-lapse imaging techniques, and quantitative image analysis using the open-source software FIJI. The approach in this manuscript outlines the quantification of competitive interactions between symbiotic Vibrio fischeri populations by measuring the change in area over time for two coincubated strains that are expressing different fluorescent proteins from stable plasmids. Alternative methods are described for optimizing this protocol in bacterial model systems that require different growth conditions. Although the assay described here uses conditions optimized for V. fischeri, this approach is highly reproducible and can easily be adapted to study competition among culturable isolates from diverse microbiomes.
    Keywords Vibrio fischeri ; computer software ; fluorescence ; fluorescence microscopy ; image analysis ; microbiome ; plasmids
    Language English
    Dates of publication 2021-0902
    Size p. e62851.
    Publishing place Journal of Visualized Experiments
    Document type Article
    ZDB-ID 2259946-0
    ISSN 1940-087X
    ISSN 1940-087X
    DOI 10.3791/62851
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Calcium Promotes T6SS-Mediated Killing and Aggregation between Competing Symbionts.

    Speare, Lauren / Jackson, Aundre / Septer, Alecia N

    Microbiology spectrum

    2022  Volume 10, Issue 6, Page(s) e0139722

    Abstract: Bacteria use a variety of strategies to exclude competitors from accessing resources, including space within a host niche. Because these mechanisms are typically costly to deploy, they are often tightly regulated for use in environments where the ... ...

    Abstract Bacteria use a variety of strategies to exclude competitors from accessing resources, including space within a host niche. Because these mechanisms are typically costly to deploy, they are often tightly regulated for use in environments where the benefits outweigh the energetic cost. The type VI secretion system (T6SS) is a competitive mechanism that allows inhibitors to kill competing microbes by physically puncturing and translocating cytotoxic effectors directly into neighboring competitor cells. Although T6SSs are encoded in both symbiotic and free-living taxa where they may be actively secreting into the extracellular milieu during growth in liquid culture, there is little evidence for bacteria engaging in T6SS-mediated, contact-dependent killing under low-viscosity liquid conditions. Here, we determined that calcium acts as a pH-dependent cue to activate the assembly of an antibacterial T6SS in a Vibrio fischeri light organ symbiont in a low-viscosity liquid medium. Moreover, competing V. fischeri isolates formed mixed-strain aggregates that promoted the contact necessary for T6SS-dependent elimination of a target population. Our findings expand our knowledge of V. fischeri T6SS ecology and identify a low-viscosity liquid condition where cells engage in contact-dependent killing.
    MeSH term(s) Calcium ; Gene Expression Regulation, Bacterial ; Type VI Secretion Systems/metabolism ; Symbiosis ; Aliivibrio fischeri/metabolism ; Bacterial Proteins/genetics
    Chemical Substances Calcium (SY7Q814VUP) ; Type VI Secretion Systems ; Bacterial Proteins
    Language English
    Publishing date 2022-12-01
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.01397-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: A Putative Lipoprotein Mediates Cell-Cell Contact for Type VI Secretion System-Dependent Killing of Specific Competitors.

    Speare, Lauren / Woo, Madison / Dunn, Anne K / Septer, Alecia N

    mBio

    2022  Volume 13, Issue 2, Page(s) e0308521

    Abstract: Interbacterial competition is prevalent in host-associated microbiota, where it can shape community structure and function, impacting host health in both positive and negative ways. However, the factors that permit bacteria to discriminate among their ... ...

    Abstract Interbacterial competition is prevalent in host-associated microbiota, where it can shape community structure and function, impacting host health in both positive and negative ways. However, the factors that permit bacteria to discriminate among their various neighbors for targeted elimination of competitors remain elusive. We identified a putative lipoprotein (TasL) in
    MeSH term(s) Aliivibrio fischeri/genetics ; Aliivibrio fischeri/metabolism ; Animals ; Decapodiformes/microbiology ; Lipoproteins/genetics ; Symbiosis ; Type VI Secretion Systems/genetics ; Type VI Secretion Systems/metabolism
    Chemical Substances Lipoproteins ; Type VI Secretion Systems
    Language English
    Publishing date 2022-04-11
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2557172-2
    ISSN 2150-7511 ; 2161-2129
    ISSN (online) 2150-7511
    ISSN 2161-2129
    DOI 10.1128/mbio.03085-21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: A subcellular biochemical model for T6SS dynamics reveals winning competitive strategies.

    Lin, Yuexia Luna / Smith, Stephanie N / Kanso, Eva / Septer, Alecia N / Rycroft, Chris H

    PNAS nexus

    2023  Volume 2, Issue 7, Page(s) pgad195

    Abstract: The type VI secretion system (T6SS) is a broadly distributed interbacterial weapon that can be used to eliminate competing bacterial populations. Although unarmed target populations are typically used to study T6SS function in vitro, bacteria most likely ...

    Abstract The type VI secretion system (T6SS) is a broadly distributed interbacterial weapon that can be used to eliminate competing bacterial populations. Although unarmed target populations are typically used to study T6SS function in vitro, bacteria most likely encounter other T6SS-armed competitors in nature. However, the connection between subcellular details of the T6SS and the outcomes of such mutually lethal battles is not well understood. Here, we incorporate biological data derived from natural competitors of
    Language English
    Publishing date 2023-06-13
    Publishing country England
    Document type Journal Article
    ISSN 2752-6542
    ISSN (online) 2752-6542
    DOI 10.1093/pnasnexus/pgad195
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates.

    Speare, Lauren / Septer, Alecia N

    Journal of visualized experiments : JoVE

    2019  , Issue 149

    Abstract: This manuscript describes a culture-based, coincubation assay for detecting and characterizing competitive interactions between two bacterial populations. This method employs stable plasmids that allow each population to be differentially tagged with ... ...

    Abstract This manuscript describes a culture-based, coincubation assay for detecting and characterizing competitive interactions between two bacterial populations. This method employs stable plasmids that allow each population to be differentially tagged with distinct antibiotic resistance capabilities and fluorescent proteins for selection and visual discrimination of each population, respectively. Here, we describe the preparation and coincubation of competing Vibrio fischeri strains, fluorescence microscopy imaging, and quantitative data analysis. This approach is simple, yields quick results, and can be used to determine whether one population kills or inhibits the growth of another population, and whether competition is mediated through a diffusible molecule or requires direct cell-cell contact. Because each bacterial population expresses a different fluorescent protein, the assay permits the spatial discrimination of competing populations within a mixed colony. Although the described methods are performed with the symbiotic bacterium V. fischeri using conditions optimized for this species, the protocol can be adapted for most culturable bacterial isolates.
    MeSH term(s) Aliivibrio fischeri/drug effects ; Aliivibrio fischeri/growth & development ; Aliivibrio fischeri/isolation & purification ; Drug Resistance, Microbial ; Plasmids ; Symbiosis
    Language English
    Publishing date 2019-07-22
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Video-Audio Media
    ZDB-ID 2259946-0
    ISSN 1940-087X ; 1940-087X
    ISSN (online) 1940-087X
    ISSN 1940-087X
    DOI 10.3791/59759
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Coincubation assay for quantifying competitive interactions between Vibrio fischeri isolates

    Speare, Lauren / Septer, Alecia N

    Journal of visualized experiments. 2019 July 22, , no. 149

    2019  

    Abstract: This manuscript describes a culture-based, coincubation assay for detecting and characterizing competitive interactions between two bacterial populations. This method employs stable plasmids that allow each population to be differentially tagged with ... ...

    Abstract This manuscript describes a culture-based, coincubation assay for detecting and characterizing competitive interactions between two bacterial populations. This method employs stable plasmids that allow each population to be differentially tagged with distinct antibiotic resistance capabilities and fluorescent proteins for selection and visual discrimination of each population, respectively. Here, we describe the preparation and coincubation of competing Vibrio fischeri strains, fluorescence microscopy imaging, and quantitative data analysis. This approach is simple, yields quick results, and can be used to determine whether one population kills or inhibits the growth of another population, and whether competition is mediated through a diffusible molecule or requires direct cell-cell contact. Because each bacterial population expresses a different fluorescent protein, the assay permits the spatial discrimination of competing populations within a mixed colony. Although the described methods are performed with the symbiotic bacterium V. fischeri using conditions optimized for this species, the protocol can be adapted for most culturable bacterial isolates.
    Keywords antibiotic resistance ; bacteria ; fluorescence microscopy ; fluorescent proteins ; image analysis ; plasmids ; Vibrio fischeri
    Language English
    Dates of publication 2019-0722
    Size p. e59759.
    Publishing place Journal of Visualized Experiments
    Document type Article
    ZDB-ID 2259946-0
    ISSN 1940-087X
    ISSN 1940-087X
    DOI 10.3791/59759
    Database NAL-Catalogue (AGRICOLA)

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