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Article ; Online: A Knockout of the IFITM3 Gene Increases the Sensitivity of WI-38 VA13 Cells to the Influenza A Virus.

Eshchenko, Natalya / Sergeeva, Mariia / Zhuravlev, Evgenii / Kudria, Kira / Goncharova, Elena / Komissarov, Andrey / Stepanov, Grigory

International journal of molecular sciences

2024 Volume 25, Issue 1

Abstract: One of the ways to regulate the sensitivity of human cells to the influenza virus is to knock out genes of the innate immune response. Promising targets for the knockout are genes of the interferon-inducible transmembrane protein (IFITM) family, in ... ...

Abstract	One of the ways to regulate the sensitivity of human cells to the influenza virus is to knock out genes of the innate immune response. Promising targets for the knockout are genes of the interferon-inducible transmembrane protein (IFITM) family, in particular the
MeSH term(s)	Humans ; Influenza A virus/genetics ; Cell Line ; Culture Media ; Endosomes ; Gene Editing ; Membrane Proteins/genetics ; RNA-Binding Proteins
Chemical Substances	Culture Media ; IFITM3 protein, human ; Membrane Proteins ; RNA-Binding Proteins
Language	English
Publishing date	2024-01-03
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms25010625
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Aging Brain, Dementia and Impaired Glymphatic Pathway: Causal Relationships.

Shirolapov, Igor / Zakharov, Alexander / Smirnova, Daria / Khivintseva, Elena / Sergeeva, Mariya

Psychiatria Danubina

2023 Volume 35, Issue Suppl 2, Page(s) 236–244

Abstract: Cellular and molecular processes that are of key importance in the development of neuroinflammation and increased cytokine response, activation of microglia and astrogliosis, contributing to the accumulation of metabolites and aberrant proteins in the ... ...

Abstract	Cellular and molecular processes that are of key importance in the development of neuroinflammation and increased cytokine response, activation of microglia and astrogliosis, contributing to the accumulation of metabolites and aberrant proteins in the brain tissue due to their overproduction and insufficient clearance, concomitant disturbance of architecture and sleep patterns are interconnected and induce brain aging with the formation its complex neurobiological mechanism. The study of these processes brings us closer to understanding the main determinants of healthy and unhealthy aging, primary prevention and preclinical diagnosis of age-related diseases, as well as to solving problems of longevity and increasing quality of life. The imbalance of homeostatic functions that support the exchange of fluids and solutes in the brain tissue is observed both in physiological aging and in the development of pathology of the nervous system with long-term consequences - from impaired synaptic signaling to the development of neurodegenerative diseases. Dementia is one of the major health problems worldwide and is very complex in terms of pathophysiology. Therefore, one of the priorities of fundamental neurobiology is to elucidate the main morbid mechanisms of Alzheimer's disease as the most common form of dementia. The hypotheses of β-amyloid and tau protein largely explain the main pathological features of Alzheimer's disease, however, there remains a need for further research on biomarkers with high validity and predictive applicability in people without cognitive impairment and clinical symptoms and in the early stages of the disease. There is a need to intensify the search for effective solutions to slow or stop the progression of the disease, especially therapeutic approaches that modify the disease at the preclinical stage, when it is most beneficial to change its course. At the same time, the discovery of aquaporin-dependent clearance pathways in the brain made it possible to identify new mechanisms underlying the etiology and progression of neurodegeneration and neuroinflammation. Glial-mediated clearance plays a fundamental role in the process of physiological aging, the development of age-related changes in the brain, and neurodegenerative processes. We analyzed 273 articles posted in PubMed database selected by keywords "glymphatic system, Alzheimer's disease, dementia, amyloid Aβ, aquaporin, aging, brain clearance". A total of 102 full-text articles were included in this review. This article presents up-to-date evidence on the causes and consequences in the study of the relationship between dysfunction of the glymphatic pathway and the accumulation of pathological proteins with insufficient excretion of toxic metabolites from the brain parenchyma, which is considered a key factor in the development of Alzheimer's dementia.
MeSH term(s)	Humans ; Alzheimer Disease/diagnosis ; Glymphatic System ; Neuroinflammatory Diseases ; Quality of Life ; Brain/pathology ; Aging ; Aquaporins/metabolism
Chemical Substances	Aquaporins
Language	English
Publishing date	2023-08-08
Publishing country	Croatia
Document type	Review ; Journal Article
ZDB-ID	1067580-2
ISSN	0353-5053
ISSN	0353-5053
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Article ; Online: Vestibular disorders following different types of head and neck trauma.

Kolev, Ognyan I / Sergeeva, M

Functional neurology

2016 Volume 31, Issue 2, Page(s) 75–80

Abstract: This review focuses on the published literature on vestibular disorders following different types of head and neck trauma. Current knowledge of the different causes and underlying mechanisms of vestibular disorders, as well as the sites of organic damage, ...

Abstract	This review focuses on the published literature on vestibular disorders following different types of head and neck trauma. Current knowledge of the different causes and underlying mechanisms of vestibular disorders, as well as the sites of organic damage, is presented. Non-organic mechanisms are also surveyed. The frequency of occurrence of vestibular symptoms, and of other accompanying subjective complaints, associated with different types of trauma is presented and related to the specific causes. Hypotheses about the pathogenesis of traumatic vestibular disorders are presented, and the knowledge derived from animal experiments is also discussed. We believe this to be a very important topic, since vestibular complaints in traumatic patients often remain undiagnosed or underestimated in clinical practice. This review article aims to suggest directions for additional research and to provide guidance to both the scientific and clinical practice communities.
MeSH term(s)	Craniocerebral Trauma/complications ; Craniocerebral Trauma/physiopathology ; Humans ; Neck Injuries/complications ; Neck Injuries/physiopathology ; Vestibular Diseases/etiology ; Vestibular Diseases/physiopathology
Language	English
Publishing date	2016-06-30
Publishing country	Italy
Document type	Journal Article ; Review
ZDB-ID	645047-7
ISSN	1971-3274 ; 0393-5264
ISSN (online)	1971-3274
ISSN	0393-5264
DOI	10.11138/fneur/2016.31.2.075
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Zs.A 2434: Show issues

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Article ; Online: Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus.

Prokhorova, Daria / Zhukova Eschenko, Natalya / Lemza, Anna / Sergeeva, Mariia / Amirkhanov, Rinat / Stepanov, Grigory

Viruses

2022 Volume 14, Issue 2

Abstract: Influenza A virus (IAV) causes a respiratory infection that affects millions of people of different age groups and can lead to acute respiratory distress syndrome. Currently, host genes, receptors, and other cellular components critical for IAV ... ...

Abstract	Influenza A virus (IAV) causes a respiratory infection that affects millions of people of different age groups and can lead to acute respiratory distress syndrome. Currently, host genes, receptors, and other cellular components critical for IAV replication are actively studied. One of the most convenient and accessible genome-editing tools to facilitate these studies is the CRISPR/Cas9 system. This tool allows for regulating the expression of both viral and host cell genes to enhance or impair viral entry and replication. This review considers the effect of the genome editing system on specific target genes in cells (human and chicken) in terms of subsequent changes in the influenza virus life cycle and the efficiency of virus particle production.
MeSH term(s)	Animals ; CRISPR-Cas Systems/genetics ; Cell Line ; Chickens ; Gene Editing/methods ; Humans ; Immunity, Cellular ; Influenza A virus/genetics ; Influenza A virus/physiology ; Virus Internalization ; Virus Replication
Language	English
Publishing date	2022-02-21
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v14020437
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Article ; Online: Climate change and public health in South America: a scoping review of governance and public engagement research.

Takahashi, Bruno / Gil Posse, Carolina / Sergeeva, Milena / Salas, María Fernanda / Wojczynski, Sydney / Hartinger, Stella / Yglesias-González, Marisol

Lancet regional health. Americas

2023 Volume 26, Page(s) 100603

Abstract: This scoping review examines peer-reviewed literature of governance and public engagement at the intersection of public health and climate change in South America. The review shows significant gaps in academic publications, particularly because health ... ...

Abstract	This scoping review examines peer-reviewed literature of governance and public engagement at the intersection of public health and climate change in South America. The review shows significant gaps in academic publications, particularly because health was mostly a secondary theme examined in the studies. The few studies about governmental interventions (e.g., policies and programs) suggest that these have not been effective. Regarding public engagement, no studies examined social media engagement with health and climate change, and only one examined news coverage. Finally, most articles focused primarily on individual countries, with few comparative or regional analyses of South America. Strategic action addressing climate change and its effects on public health needs to be based on empirical evidence.
Language	English
Publishing date	2023-09-26
Publishing country	England
Document type	Journal Article ; Review
ISSN	2667-193X
ISSN (online)	2667-193X
DOI	10.1016/j.lana.2023.100603
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Article: Intranasal Immunization with the Influenza A Virus Encoding Truncated NS1 Protein Protects Mice from Heterologous Challenge by Restraining the Inflammatory Response in the Lungs.

Vasilyev, Kirill / Shurygina, Anna-Polina / Sergeeva, Maria / Stukova, Marina / Egorov, Andrej

Microorganisms

2021 Volume 9, Issue 4

Abstract: Influenza viruses with an impaired NS1 protein are unable to antagonize the innate immune system and, therefore, are highly immunogenic because of the self-adjuvating effect. Hence, NS1-mutated viruses are considered promising candidates for the ... ...

Abstract	Influenza viruses with an impaired NS1 protein are unable to antagonize the innate immune system and, therefore, are highly immunogenic because of the self-adjuvating effect. Hence, NS1-mutated viruses are considered promising candidates for the development of live-attenuated influenza vaccines and viral vectors for intranasal administration. We investigated whether the immunogenic advantage of the virus expressing only the N-terminal half of the NS1 protein (124 a.a.) can be translated into the induction of protective immunity against a heterologous influenza virus in mice. We found that immunization with either the wild-type A/PR/8/34 (H1N1) influenza strain (A/PR8/NSfull) or its NS1-shortened counterpart (A/PR8/NS124) did not prevent the viral replication in the lungs after the challenge with the A/Aichi/2/68 (H3N2) virus. However, mice immunized with the NS1-shortened virus were better protected from lethality after the challenge with the heterologous virus. Besides showing the enhanced influenza-specific CD8
Language	English
Publishing date	2021-03-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms9040690
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Article ; Online: Code Red for Health

Yglesias-González, Marisol / Palmeiro-Silva, Yasna / Sergeeva, Milena / Cortés, Sandra / Hurtado-Epstein, Andrea / Buss, Daniel F / Hartinger, Stella M

Lancet regional health. Americas

2022 Volume 11, Page(s) 100248

Language	English
Publishing date	2022-04-20
Publishing country	England
Document type	Journal Article ; Review
ISSN	2667-193X
ISSN (online)	2667-193X
DOI	10.1016/j.lana.2022.100248
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Article ; Online: Analysis of Expression Pattern of snoRNAs in Human Cells A549 Infected by Influenza A Virus.

Zhuravlev, Evgenii / Sergeeva, Mariia / Malanin, Sergey / Amirkhanov, Rinat / Semenov, Dmitriy / Grigoryeva, Tatiana / Komissarov, Andrey / Stepanov, Grigory

International journal of molecular sciences

2022 Volume 23, Issue 22

Abstract: Small nucleolar RNAs (snoRNAs) are a highly expressed class of non-coding RNAs known for their role in guiding post-transcriptional modifications of ribosomal RNAs and small nuclear RNAs. Emerging studies suggest that snoRNAs are also implicated in ... ...

Abstract	Small nucleolar RNAs (snoRNAs) are a highly expressed class of non-coding RNAs known for their role in guiding post-transcriptional modifications of ribosomal RNAs and small nuclear RNAs. Emerging studies suggest that snoRNAs are also implicated in regulating other vital cellular processes, such as pre-mRNA splicing and 3'-processing of mRNAs, and in the development of cancer and viral infections. There is an emerging body of evidence for specific snoRNA's involvement in the optimal replication of RNA viruses. In order to investigate the expression pattern of snoRNAs during influenza A viral infection, we performed RNA sequencing analysis of the A549 human cell line infected by influenza virus A/Puerto Rico/8/1934 (H1N1). We identified 66 that were upregulated and 55 that were downregulated in response to influenza A virus infection. The increased expression of most C/D-box snoRNAs was associated with elevated levels of 5'- and 3'-short RNAs derived from this snoRNA. Analysis of the poly(A)+ RNA sequencing data indicated that most of the differentially expressed snoRNAs synthesis was not correlated with the corresponding host genes expression. Furthermore, influenza A viral infection led to an imbalance in the expression of genes responsible for C/D small nucleolar ribonucleoprotein particles' biogenesis. In summary, our results indicate that the expression pattern of snoRNAs in A549 cells is significantly altered during influenza A viral infection.
MeSH term(s)	Humans ; RNA, Small Nucleolar/genetics ; RNA, Small Nucleolar/metabolism ; Influenza A virus/genetics ; Influenza A Virus, H1N1 Subtype/genetics ; Influenza A Virus, H1N1 Subtype/metabolism ; Influenza, Human/genetics ; RNA, Ribosomal
Chemical Substances	RNA, Small Nucleolar ; RNA, Ribosomal
Language	English
Publishing date	2022-11-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms232213666
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Article ; Online: Rapid Assessment of Neutralizing Antibodies Using Influenza Viruses with a Luciferase Reporter

Sergeeva, M. V. / Pulkina, A. A. / Romanovskaya-Romanko, E. A. / Mustafaeva, A. S. / Egorov, A. Yu / Stukova, M. A.

Appl Biochem Microbiol. 2022 Dec., v. 58, no. 7 p.878-886

2022

Abstract: Influenza viruses cause acute respiratory infections, especially in the autumn–winter period. They are characterized by a high mutation frequency and cause annual seasonal epidemics. The detection of antibodies that neutralize the virus is an important ... ...

Abstract	Influenza viruses cause acute respiratory infections, especially in the autumn–winter period. They are characterized by a high mutation frequency and cause annual seasonal epidemics. The detection of antibodies that neutralize the virus is an important criterion in the assessment of population immunity and the influenza vaccine effectiveness. In this study, a method for determining the titer of virus-neutralizing antibodies in blood serum has been developed. A new test called the luciferase neutralization assay uses a bioluminescent signal for detection. The assay is based on engineered influenza reporter viruses with various surface antigens and a nanoluciferase reporter protein in the NS1 reading frame. Using the developed method, we studied paired sera of volunteers obtained before and after vaccination. The proposed assay was compared with the conventional antibody assessment methods (microneutralization and hemagglutination inhibition assay); a high degree of correlation was observed. At the same time, the use of the luciferase neutralization assay made it possible to reduce the time required for the analysis and to simplify the detection procedure.
Keywords	antibodies ; bioluminescence ; blood serum ; hemagglutination inhibition test ; immunity ; influenza ; influenza vaccines ; luciferase ; mutation rate ; neutralization tests ; rapid methods ; vaccination ; viruses
Language	English
Dates of publication	2022-12
Size	p. 878-886.
Publishing place	Pleiades Publishing
Document type	Article ; Online
ZDB-ID	412550-2
ISSN	1608-3024 ; 0003-6838
ISSN (online)	1608-3024
ISSN	0003-6838
DOI	10.1134/S0003683822070067
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: IgGκ Signal Peptide Enhances the Efficacy of an Influenza Vector Vaccine against Respiratory Syncytial Virus Infection in Mice.

Pulkina, Anastasia / Vasilyev, Kirill / Muzhikyan, Arman / Sergeeva, Mariia / Romanovskaya-Romanko, Ekaterina / Shurygina, Anna-Polina / Shuklina, Marina / Vasin, Andrey / Stukova, Marina / Egorov, Andrej

International journal of molecular sciences

2023 Volume 24, Issue 14

Abstract: Intranasal vaccination using influenza vectors is a promising approach to developing vaccines against respiratory pathogens due to the activation of the mucosa-associated immune response. However, there is no clear evidence of a vector design that could ... ...

Abstract	Intranasal vaccination using influenza vectors is a promising approach to developing vaccines against respiratory pathogens due to the activation of the mucosa-associated immune response. However, there is no clear evidence of a vector design that could be considered preferable. To find the optimal structure of an influenza vector with a modified NS genomic segment, we constructed four vector expressing identical transgene sequences inherited from the F protein of the respiratory syncytial virus (RSV). Two vectors were designed aiming at transgene accumulation in the cytosol. Another two were supplemented with an IgGκ signal peptide prior to the transgene for its extracellular delivery. Surprisingly, adding the IgGκ substantially enhanced the T-cell immune response to the CD8 epitope of the transgene. Moreover, this strategy allowed us to obtain a better protection of mice from the RSV challenge after a single intranasal immunization. Protection was achieved without antibodies, mediated by a balanced T-cell immune response including the formation of the RSV specific effector CD8+ IFNγ+/IL10+-producing cells and the accumulation of Treg cells preventing immunopathology in the lungs of infected mice. In addition to the presented method for optimizing the influenza vector, our results highlight the possibility of achieving protection against RSV through a respiratory-associated T-cell immune response alone.
MeSH term(s)	Animals ; Mice ; Humans ; Respiratory Syncytial Virus Infections ; Influenza Vaccines ; Influenza, Human ; Antibodies, Viral ; Respiratory Syncytial Virus, Human/genetics ; Respiratory Syncytial Virus Vaccines ; Mice, Inbred BALB C
Chemical Substances	Influenza Vaccines ; Antibodies, Viral ; Respiratory Syncytial Virus Vaccines
Language	English
Publishing date	2023-07-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms241411445
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