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  1. Article ; Online: APPLICATION OF NUCLEIC ACID AMPLIFICATION TESTS IN MANAGING COVID-19 PANDEMIC

    Serhiy Souchelnytskyi / Nazariy Souchelnytskyi

    Праці Наукового товариства імені Шевченка. Медичні науки, Vol 62, Iss

    2020  Volume 2

    Abstract: Background. COVID-19 pandemic highlighted an importance of sensitive and specific tests that would be cost-efficient, fast and scalable. There are more than 200 COVID-19 detection tests available worldwide, with every country developing own assays. ... ...

    Abstract Background. COVID-19 pandemic highlighted an importance of sensitive and specific tests that would be cost-efficient, fast and scalable. There are more than 200 COVID-19 detection tests available worldwide, with every country developing own assays. Sample collection, preparation for a test, tests itself and interpretation of results have a strong impact on the clinical value of the testing. Diversity of tests and workflows requires an analysis of their performance in clinics. Methods. Literature review, analysis of clinical reports, online resources, public and commercial reports were used to collect information about the tests. Collected information was processed for extraction of relevant to this review information. Results. Here we review COVID-19 detection tests that are based on amplification of nucleic acids. The tests employ polymerase chain reaction (PCR) or loop-mediated isothermal amplification (LAMP). Clinical value of the tests depends on used technologies that differ for LAMP, real-time and standard PCR methods. Diversity of sample preparation protocols, different designs of the tests, used chemistries and protocols have significant impact on performance of the tests. Tailoring of a testing workflow to available infrastructure and selection of the most efficient combination of tests and protocols for each step in a testing workflow is crucial for the success. Conclusion. Reviewed here strong and weak sides of different tests and protocols can be of help in selection of a testing workflow for achieving maximal clinical utility.
    Keywords covid-19 ; detection test ; pcr ; lamp ; Medicine ; R
    Subject code 519
    Language English
    Publishing date 2020-09-01T00:00:00Z
    Publisher Shevchenko Scientific Society
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: COVID-19 engages clinical markers for the management of cancer and cancer-relevant regulators of cell proliferation, death, migration, and immune response

    Serhiy Souchelnytskyi / Andriy Nera / Nazariy Souchelnytskyi

    Scientific Reports, Vol 11, Iss 1, Pp 1-

    2021  Volume 11

    Abstract: Abstract Clinical reports show that the management of cancer patients infected with SARS-CoV-2 requires modifications. Understanding of cancer-relevant mechanisms engaged by the virus is essential for the evidence-based management of cancer. The network ... ...

    Abstract Abstract Clinical reports show that the management of cancer patients infected with SARS-CoV-2 requires modifications. Understanding of cancer-relevant mechanisms engaged by the virus is essential for the evidence-based management of cancer. The network of SARS-CoV-2 regulatory mechanisms was used to study potential engagement of oncogenes, tumor suppressors, other regulators of tumorigenesis and clinical markers used in the management of cancer patients. Our network analysis confirms links between COVID-19 and tumorigenesis that were predicted in epidemiological reports. The COVID-19 network shows the involvement of tumorigenesis regulators and clinical markers. Regulators of cell proliferation, death, migration, and the immune system were retrieved. Examples are pathways initiated by EGF, VEGF, TGFβ and FGF. The SARS-CoV-2 network engages markers for diagnosis, prognosis and selection of treatment. Intersection with cancer diagnostic signatures supports a potential impact of the virus on tumorigenesis. Clinical observations show the diversity of symptoms correlating with biological processes and types of cells engaged by the virus, e.g. epithelial, endothelial, smooth muscle, glial and immune system cells. Our results describe an extensive engagement of cancer-relevant mechanisms and clinical markers by COVID-19. Engagement by the virus of clinical markers provides a rationale for clinical decisions based on these markers.
    Keywords Medicine ; R ; Science ; Q
    Subject code 610
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: CELL-BASED DIAGNOSTIC OF BRONCHIAL CARCINOMA

    Nazariy Souchelnytskyi / Andriy Kost / Serhiy Souchelnytskyi

    Праці Наукового товариства імені Шевченка. Медичні науки, Vol 59, Iss

    CASE STUDY OF A DIAGNOSTIC VALUE OF TESTS OF CIRCULATING TUMOR CELLS AND ORGANOID CULTURE

    2020  Volume 1

    Abstract: Diagnostic based on analysis of living tumor cells is frequently used in oncology. Circulating tumor cells in the blood and cells obtained from a tumor biopsy are used to access their carcinogenic properties and subsequently to predict possible ... ...

    Abstract Diagnostic based on analysis of living tumor cells is frequently used in oncology. Circulating tumor cells in the blood and cells obtained from a tumor biopsy are used to access their carcinogenic properties and subsequently to predict possible development of the disease. Here we report use of these two tests to assess aggressiveness and metastatic potential of a bronchial adenocarcinoma. The circulating tumor cells test was negative, no circulating cells was observed. It indicates that there was no metastatic spread. However, test with the surgery biopsy showed presence of aggressive cellular clones. The tumor cells from the biopsy proliferated and spread from the cultured tissue. Moreover, the tumor cells formed colonies of cells which lost contact inhibition. This is an indication of aggressive carcinogenic features of the cells in tumor organoids. Combination of both tests showed that the local tumor had an aggressive phenotype, but no detectable spreading of cells. Therefore, these tests support a management plan with removal the primary tumor and regular monitoring, without need of an extensive chemotherapy.
    Keywords cancer ; adenocarcinoma ; circulating tumor cells ; organ culture ; Medicine ; R
    Subject code 610
    Language English
    Publishing date 2020-04-01T00:00:00Z
    Publisher Shevchenko Scientific Society
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: A role of TGFß1 dependent 14-3-3σ phosphorylation at Ser69 and Ser74 in the regulation of gene transcription, stemness and radioresistance.

    Olena Zakharchenko / Monica Cojoc / Anna Dubrovska / Serhiy Souchelnytskyi

    PLoS ONE, Vol 8, Iss 5, p e

    2013  Volume 65163

    Abstract: Transforming growth factor-β (TGFβ) is a potent regulator of tumorigenesis, although mechanisms defining its tumor suppressing and tumor promoting activities are not understood. Here we describe phosphoproteome profiling of TGFβ signaling in mammary ... ...

    Abstract Transforming growth factor-β (TGFβ) is a potent regulator of tumorigenesis, although mechanisms defining its tumor suppressing and tumor promoting activities are not understood. Here we describe phosphoproteome profiling of TGFβ signaling in mammary epithelial cells, and show that 60 identified TGFβ-regulated phosphoproteins form a network with scale-free characteristics. The network highlighted interactions, which may distribute signaling inputs to regulation of cell proliferation, metabolism, differentiation and cell organization. In this report, we identified two novel and TGFβ-dependent phosphorylation sites of 14-3-3σ, i.e. Ser69 and Ser74. We observed that 14-3-3σ phosphorylation is a feed-forward mechanism in TGFβ/Smad3-dependent transcription. TGFβ-dependent 14-3-3σ phosphorylation may provide a scaffold for the formation of the protein complexes which include Smad3 and p53 at the Smad3-specific CAGA element. Furthermore, breast tumor xenograft studies in mice and radiobiological assays showed that phosphorylation of 14-3-3σ at Ser69 and Ser74 is involved in regulation of cancer progenitor population and radioresistance in breast cancer MCF7 cells. Our data suggest that TGFβ-dependent phosphorylation of 14-3-3σ orchestrates a functional interaction of TGFβ/Smad3 with p53, plays a role in the maintenance of cancer stem cells and could provide a new potential target for intervention in breast cancer.
    Keywords Medicine ; R ; Science ; Q
    Subject code 571
    Language English
    Publishing date 2013-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: Proteomic analysis of the endometrium during early pregnancy in the domestic pig

    Kolakowska, Justyna / Serhiy Souchelnytskyi / Ravi Kanth Rao Saini / Anita Franczak

    Reproduction, fertility, and development. 2017, v. 29, no. 11

    2017  

    Abstract: Reproductive processes in domestic pigs have been studied extensively. Pigs are one of the main sources of meat for human consumption and are an established model for investigations into mammalian, including human, reproductive physiology. Studies of the ...

    Abstract Reproductive processes in domestic pigs have been studied extensively. Pigs are one of the main sources of meat for human consumption and are an established model for investigations into mammalian, including human, reproductive physiology. Studies of the uterus during early pregnancy will lead to a better understanding of mechanisms governing pregnancy. Proteomics provides the possibility to explore endometrial functions in an unbiased way. The aim of the study was to compare endometrium harvested from Days 12–13 and 15–16 of pregnancy with the corresponding days of the oestrous cycle. We identified endometrial proteins that are unique to the early stages of pregnancy (Days 12–13 and 15–16). Twenty-one proteins were identified that were uniquely expressed on the selected days of pregnancy or the oestrous cycle. Out of 21 identified proteins, 14 referred to the pregnancy periods. Systemic analysis of the identified proteins revealed cell adhesion and cytoskeletal organisation as two of the major functions, both of which are important for the establishment and maintenance of pregnancy. Thrombospondin 1 expression was validated using western blotting analysis and the results suggest its involvement in the adhesiveness of the embryo during the peri-implantation period in pigs.
    Keywords Western blotting ; adhesion ; cell adhesion ; cytoskeleton ; endometrium ; estrous cycle ; models ; pregnancy ; proteins ; proteomics ; swine
    Language English
    Size p. 2255-2268.
    Publishing place CSIRO Publishing
    Document type Article
    ZDB-ID 1019913-5
    ISSN 1448-5990 ; 1031-3613
    ISSN (online) 1448-5990
    ISSN 1031-3613
    DOI 10.1071/RD16435
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Optimized Protocol for Protein Extraction from the Breast Tissue that is Compatible with Two-Dimensional Gel Electrophoresis

    Olena Zakharchenko, / Christina Greenwood / Louise Alldridge / Serhiy Souchelnytskyi

    Breast Cancer : Basic and Clinical Research, Vol 2011, Iss 5, Pp 37-

    2011  Volume 42

    Keywords Neoplasms. Tumors. Oncology. Including cancer and carcinogens ; RC254-282 ; Internal medicine ; RC31-1245 ; Medicine ; R ; DOAJ:Oncology ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
    Language English
    Publishing date 2011-03-01T00:00:00Z
    Publisher Libertas Academica
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Identification of a 48 kDa form of unconventional myosin 1c in blood serum of patients with autoimmune diseases

    Severyn Myronovkij / Nazar Negrych / Tetyana Nehrych / Maria J. Redowicz / Serhiy Souchelnytskyi / Rostyslav Stoika / Yuriy Kit

    Biochemistry and Biophysics Reports, Vol 5, Iss C, Pp 175-

    2016  Volume 179

    Abstract: We searched for protein markers present in blood serum of multiple sclerosis (MS), rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) patients in comparison to healthy human individuals. We used precipitation/extraction methods and MALDI ... ...

    Abstract We searched for protein markers present in blood serum of multiple sclerosis (MS), rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) patients in comparison to healthy human individuals. We used precipitation/extraction methods and MALDI TOF/TOF mass spectrometry, and identified a protein with Mr ~46 kDa as a fragment of human unconventional myosin IC isoform b (Myo1C). Western blotting with specific anti-human Myo1C antibodies confirmed the identity. Screening of blood serum samples from different autoimmune patients for the presence of Myo1c revealed its high level in MS and RA patients, relatively low level in SLE patients, and undetected in healthy donors. These data are suggesting that the level of p46 Myo1C in blood serum is a potential marker for testing of autoimmune diseases.
    Keywords Human blood serum ; Autoimmune diseases ; TCA-extracted proteins ; Mass-spectrometry ; Biology (General) ; QH301-705.5 ; Biochemistry ; QD415-436
    Subject code 610
    Language English
    Publishing date 2016-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Characterization of the SNAG and SLUG domains of Snail2 in the repression of E-cadherin and EMT induction

    Patricia Molina-Ortiz / Ana Villarejo / Matthew MacPherson / Vanesa Santos / Amalia Montes / Serhiy Souchelnytskyi / Francisco Portillo / Amparo Cano

    PLoS ONE, Vol 7, Iss 5, p e

    modulation by serine 4 phosphorylation.

    2012  Volume 36132

    Abstract: Snail1 and Snail2, two highly related members of the Snail superfamily, are direct transcriptional repressors of E-cadherin and EMT inducers. Previous comparative gene profiling analyses have revealed important differences in the gene expression pattern ... ...

    Abstract Snail1 and Snail2, two highly related members of the Snail superfamily, are direct transcriptional repressors of E-cadherin and EMT inducers. Previous comparative gene profiling analyses have revealed important differences in the gene expression pattern regulated by Snail1 and Snail2, indicating functional differences between both factors. The molecular mechanism of Snail1-mediated repression has been elucidated to some extent, but very little is presently known on the repression mediated by Snail2. In the present work, we report on the characterization of Snail2 repression of E-cadherin and its regulation by phosphorylation. Both the N-terminal SNAG and the central SLUG domains of Snail2 are required for efficient repression of the E-cadherin promoter. The co-repressor NCoR interacts with Snail2 through the SNAG domain, while CtBP1 is recruited through the SLUG domain. Interestingly, the SNAG domain is absolutely required for EMT induction while the SLUG domain plays a negative modulation of Snail2 mediated EMT. Additionally, we identify here novel in vivo phosphorylation sites at serine 4 and serine 88 of Snail2 and demonstrate the functional implication of serine 4 in the regulation of Snail2-mediated repressor activity of E-cadherin and in Snail2 induction of EMT.
    Keywords Medicine ; R ; Science ; Q
    Subject code 572
    Language English
    Publishing date 2012-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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