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  1. Article ; Online: The rat Downunder (Du) coat color mutation is associated with eye anomalies and embryonic lethality and maps to a 3.9-Mb region on chromosome 3.

    Hieu, Hoang Trung / Tanaka, Miyuu / Kuwamura, Mitsuru / Mashimo, Tomoji / Serikawa, Tadao / Kuramoto, Takashi

    Experimental animals

    2022  Volume 72, Issue 1, Page(s) 88–94

    Abstract: Rodent coat color genes have been studied as a bioresource to understand developmental and cellular processes. The Downunder rat is a fancy variety with a marking on its belly that runs from the neck to the breech and appears to mirror the dorsal hooded ... ...

    Abstract Rodent coat color genes have been studied as a bioresource to understand developmental and cellular processes. The Downunder rat is a fancy variety with a marking on its belly that runs from the neck to the breech and appears to mirror the dorsal hooded marking. Here, we established a congenic strain carrying the Downunder (Du) gene in an F344 genetic background. In addition to the ventral marking, Du/+ rats exhibit anophthalmia or microphthalmia with incomplete penetrance. Du/Du embryos die in the early stages of organogenesis. Genetic linkage analysis mapped the Du gene to rat chromosome 3 and haplotype mapping with congenic rats localized the Du locus to a 3.9-Mb region. The Du locus includes two functional genes, glycosyltransferase-like domain-containing 1 (Gtdc1) and zinc finger E-box binding homeobox 2 (Zeb2). Although we found no functional variation within any of Zeb2's exons or intron-exon boundaries, Zeb2 mRNA levels were significantly lower in Du/+ rats compared with wild-type rats. It is known that melanocyte-specific Zeb2 deletion results in the congenital loss of hair pigmentation in mice. Taken together, our results indicate that the Du mutation exerts pleiotropic effects on hair pigmentation, eye morphology, and development. Moreover, the Zeb2 gene is a strong candidate for the Du mutation.
    MeSH term(s) Humans ; Rats ; Mice ; Animals ; Chromosomes, Human, Pair 3 ; Phenotype ; Rats, Inbred F344 ; Mutation ; Pigmentation/genetics ; Glycosyltransferases/genetics
    Chemical Substances GTDC1 protein, human (EC 2.4.-) ; Glycosyltransferases (EC 2.4.-)
    Language English
    Publishing date 2022-09-19
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 2088228-2
    ISSN 1881-7122 ; 1341-1357 ; 0007-5124
    ISSN (online) 1881-7122
    ISSN 1341-1357 ; 0007-5124
    DOI 10.1538/expanim.22-0086
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  2. Article: Imbalance of glutamatergic and GABAergic neurotransmission in audiogenic seizure-susceptible L

    Kinboshi, Masato / Shimizu, Saki / Tokudome, Kentaro / Mashimo, Tomoji / Serikawa, Tadao / Ito, Hidefumi / Takahashi, Ryosuke / Ikeda, Akio / Ohno, Yukihiro

    Heliyon

    2023  Volume 9, Issue 7, Page(s) e17984

    Abstract: Leucine-rich glioma-inactivated ... ...

    Abstract Leucine-rich glioma-inactivated 1
    Language English
    Publishing date 2023-07-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2023.e17984
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  3. Article: A missense mutation in the

    Tanaka, Miyuu / Fujikawa, Ryoko / Sekiguchi, Takahiro / Hernandez, Jason / Johnson, Oleta T / Tanaka, Daisuke / Kumafuji, Kenta / Serikawa, Tadao / Hoang Trung, Hieu / Hattori, Kosuke / Mashimo, Tomoji / Kuwamura, Mitsuru / Gestwicki, Jason E / Kuramoto, Takashi

    Frontiers in neuroscience

    2024  Volume 18, Page(s) 1263724

    Abstract: Neuroaxonal dystrophy (NAD) is a neurodegenerative disease characterized by spheroid (swollen axon) formation in the nervous system. In the present study, we focused on a newly established autosomal recessive mutant strain of F344- ...

    Abstract Neuroaxonal dystrophy (NAD) is a neurodegenerative disease characterized by spheroid (swollen axon) formation in the nervous system. In the present study, we focused on a newly established autosomal recessive mutant strain of F344-
    Language English
    Publishing date 2024-02-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2411902-7
    ISSN 1662-453X ; 1662-4548
    ISSN (online) 1662-453X
    ISSN 1662-4548
    DOI 10.3389/fnins.2024.1263724
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  4. Article: [Function of synaptic vesicle protein 2A (SV2A) as a novel therapeutic target for epilepsy].

    Tokudome, Kentaro / Shimizu, Saki / Serikawa, Tadao / Ohno, Yukihiro

    Nihon yakurigaku zasshi. Folia pharmacologica Japonica

    2018  Volume 152, Issue 6, Page(s) 275–280

    Abstract: Epilepsy is a chronic neurologic disease characterized by recurrent seizures, affecting nearly 1% of the population. Synaptic vesicle protein 2A (SV2A) is a membrane protein specifically expressed in synaptic vesicles and is now implicated in the ... ...

    Abstract Epilepsy is a chronic neurologic disease characterized by recurrent seizures, affecting nearly 1% of the population. Synaptic vesicle protein 2A (SV2A) is a membrane protein specifically expressed in synaptic vesicles and is now implicated in the pathogenesis of epileptic disorders. This is because 1) Sv2a-knockout mice exhibit severe seizures, 2) SV2A serves as a specific binding site for certain antiepileptics (e.g., levetiracetam and its analogues) and 3) the SV2A expression changes under various epileptic conditions both in animals (e.g., kindling) and humans (e.g., intractable temporal lobe epilepsy and focal cortical dysplasia). Furthermore, it has been shown that a missense mutation in the SV2A gene caused intractable epilepsy, involuntary movements and developmental retardation, indicating a causative role of SV2A dysfunction in epilepsy. In order to explore the mechanism of SV2A in modulating development of epileptogenesis, we recently developed a novel rat model (Sv2a
    MeSH term(s) Animals ; Epilepsy ; Humans ; Kindling, Neurologic ; Membrane Glycoproteins ; Mice ; Nerve Tissue Proteins ; Rats ; Synaptic Vesicles
    Chemical Substances Membrane Glycoproteins ; Nerve Tissue Proteins ; Sv2a protein, rat ; SV2A protein, human (148845-93-6)
    Language Japanese
    Publishing date 2018-12-05
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 1097532-9
    ISSN 1347-8397 ; 0015-5691
    ISSN (online) 1347-8397
    ISSN 0015-5691
    DOI 10.1254/fpj.152.275
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    Zs.A 439: Show issues Location:
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  5. Article ; Online: Characterization of novel, severely immunodeficient Prkdc

    Takagi, Yumie / Sudo, Katsuko / Yamaguchi, Sachiko / Urata, Shuzo / Ohno, Tatsukuni / Hirose, Sachiko / Matsumoto, Kiyoshi / Kuramoto, Takashi / Serikawa, Tadao / Yasuda, Jiro / Ikutani, Masashi / Nakae, Susumu

    Biochemical and biophysical research communications

    2023  Volume 678, Page(s) 193–199

    Abstract: Severely immunodeficient mice are useful for understanding the pathogenesis of certain tumors and for developing therapeutic agents for such tumors. In addition, engraftment of these mice with human hematopoietic cells can yield information that helps us ...

    Abstract Severely immunodeficient mice are useful for understanding the pathogenesis of certain tumors and for developing therapeutic agents for such tumors. In addition, engraftment of these mice with human hematopoietic cells can yield information that helps us understand the in vivo molecular mechanisms underlying actual human viral infections. In our present research, we discovered a novel, severely immunodeficient strain of mice having a mutation in exon 57 of the Prkdc gene (Prkdc
    MeSH term(s) Humans ; Animals ; Mice ; Immunity, Innate ; Immunologic Deficiency Syndromes ; Killer Cells, Natural ; B-Lymphocytes ; Cell Line, Tumor ; DNA-Binding Proteins ; DNA-Activated Protein Kinase
    Chemical Substances Prkdc protein, mouse (EC 2.7.11.1) ; DNA-Binding Proteins ; DNA-Activated Protein Kinase (EC 2.7.11.1)
    Language English
    Publishing date 2023-08-26
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 205723-2
    ISSN 1090-2104 ; 0006-291X ; 0006-291X
    ISSN (online) 1090-2104 ; 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2023.08.055
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  6. Article: [Rat genome sequencing and rat resources in Japan].

    Serikawa, Tadao

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme

    2004  Volume 49, Issue 13, Page(s) 2139–2148

    MeSH term(s) Animals ; Disease Models, Animal ; Genetic Predisposition to Disease ; Genetic Research ; Genome ; Japan ; Rats/genetics ; Sequence Analysis, DNA
    Language Japanese
    Publishing date 2004-10
    Publishing country Japan
    Document type Journal Article ; Review
    ZDB-ID 391163-9
    ISSN 0039-9450
    ISSN 0039-9450
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  7. Article ; Online: Colourful history of Japan's rat resources.

    Serikawa, Tadao

    Nature

    2004  Volume 429, Issue 6987, Page(s) 15

    MeSH term(s) Animals ; Databases, Factual ; Hair Color ; Japan ; Mice ; Models, Animal ; Phenotype ; Rats ; Rats, Inbred Strains
    Language English
    Publishing date 2004-05-06
    Publishing country England
    Document type Comment ; Letter
    ZDB-ID 120714-3
    ISSN 1476-4687 ; 0028-0836
    ISSN (online) 1476-4687
    ISSN 0028-0836
    DOI 10.1038/429015b
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  8. Article ; Online: Successful long-term preservation of rat sperm by freeze-drying.

    Kaneko, Takehito / Serikawa, Tadao

    PloS one

    2012  Volume 7, Issue 4, Page(s) e35043

    Abstract: Background: Freeze-drying sperm has been developed as a new preservation method where liquid nitrogen is no longer necessary. An advantage of freeze-drying sperm is that it can be stored at 4 °C and transported at room temperature. Although the ... ...

    Abstract Background: Freeze-drying sperm has been developed as a new preservation method where liquid nitrogen is no longer necessary. An advantage of freeze-drying sperm is that it can be stored at 4 °C and transported at room temperature. Although the successful freeze-drying of sperm has been reported in a number of animals, the possibility of long-term preservation using this method has not yet been studied.
    Methodology/principal findings: Offspring were obtained from oocytes fertilized with rat epididymal sperm freeze-dried using a solution containing 10 mM Tris and 1 mM EDTA adjusted to pH 8.0. Tolerance of testicular sperm to freeze-drying was increased by pre-treatment with diamide. Offspring with normal fertility were obtained from oocytes fertilized with freeze-dried epididymal sperm stored at 4 °C for 5 years.
    Conclusions and significance: Sperm with -SS- cross-linking in the thiol-disulfide of their protamine were highly tolerant to freeze-drying, and the fertility of freeze-dried sperm was maintained for 5 years without deterioration. This is the first report to demonstrate the successful freeze-drying of sperm using a new and simple method for long-term preservation.
    MeSH term(s) Animals ; Cryopreservation/methods ; Diamide/administration & dosage ; Edetic Acid/administration & dosage ; Female ; Fertility ; Freeze Drying ; Male ; Rats ; Semen Preservation/methods ; Tromethamine/administration & dosage
    Chemical Substances Tromethamine (023C2WHX2V) ; Diamide (10465-78-8) ; Edetic Acid (9G34HU7RV0)
    Language English
    Publishing date 2012-04-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0035043
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  9. Article ; Online: Long-term preservation of freeze-dried mouse spermatozoa.

    Kaneko, Takehito / Serikawa, Tadao

    Cryobiology

    2012  Volume 64, Issue 3, Page(s) 211–214

    Abstract: Many genetically engineered mice strains have been generated worldwide and sperm preservation is a valuable method for storing these strains as genetic resources. Freeze-drying is a useful sperm preservation method because it requires neither liquid ... ...

    Abstract Many genetically engineered mice strains have been generated worldwide and sperm preservation is a valuable method for storing these strains as genetic resources. Freeze-drying is a useful sperm preservation method because it requires neither liquid nitrogen nor dry ice for preservation and transportation. We report here successful long-term preservation at 4 °C of mouse spermatozoa freeze-dried using a simple buffer solution (10mM Tris, 1mM EDTA, pH 8.0). Offspring with fertility were obtained from oocytes fertilized with freeze-dried spermatozoa from C57BL/6 and B6D2F1 mouse strains stored at 4 °C for 3 years. This freeze-drying method is a safe and economical tool for the biobanking of valuable mouse strains.
    MeSH term(s) Animals ; Biological Specimen Banks ; Buffers ; Edetic Acid/chemistry ; Embryo Implantation ; Embryo Transfer ; Female ; Fertility/physiology ; Freeze Drying/economics ; Freeze Drying/methods ; Male ; Mice ; Mice, Inbred C57BL ; Oocytes/physiology ; Semen Preservation/economics ; Semen Preservation/methods ; Sperm Injections, Intracytoplasmic ; Spermatozoa/physiology ; Time Factors
    Chemical Substances Buffers ; Edetic Acid (9G34HU7RV0)
    Language English
    Publishing date 2012-06
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80098-3
    ISSN 1090-2392 ; 0011-2240
    ISSN (online) 1090-2392
    ISSN 0011-2240
    DOI 10.1016/j.cryobiol.2012.01.010
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  10. Article: Long-term preservation of freeze-dried mouse spermatozoa

    Kaneko, Takehito / Serikawa, Tadao

    Cryobiology. 2012 June, v. 64, no. 3

    2012  

    Abstract: Many genetically engineered mice strains have been generated worldwide and sperm preservation is a valuable method for storing these strains as genetic resources. Freeze-drying is a useful sperm preservation method because it requires neither liquid ... ...

    Abstract Many genetically engineered mice strains have been generated worldwide and sperm preservation is a valuable method for storing these strains as genetic resources. Freeze-drying is a useful sperm preservation method because it requires neither liquid nitrogen nor dry ice for preservation and transportation. We report here successful long-term preservation at 4°C of mouse spermatozoa freeze-dried using a simple buffer solution (10mM Tris, 1mM EDTA, pH 8.0). Offspring with fertility were obtained from oocytes fertilized with freeze-dried spermatozoa from C57BL/6 and B6D2F1 mouse strains stored at 4°C for 3years. This freeze-drying method is a safe and economical tool for the biobanking of valuable mouse strains.
    Keywords EDTA (chelating agent) ; dry ice ; freeze drying ; genetic resources ; mice ; nitrogen ; oocytes ; pH ; progeny ; spermatozoa ; transportation
    Language English
    Dates of publication 2012-06
    Size p. 211-214.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 80098-3
    ISSN 1090-2392 ; 0011-2240
    ISSN (online) 1090-2392
    ISSN 0011-2240
    DOI 10.1016/j.cryobiol.2012.01.010
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