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  1. Article ; Online: To Study the Histomorphological Changes in Cerebellar Purkinje Cells after Exposure to Fine Particulate Matter in C57BL/6J Mice

    Saima Saleem / Shabnam Hamid / Abdul Basit Jilani / Sana Malik / Saima Mumtaz Khatak

    Journal of Islamic International Medical College, Vol 18, Iss 2, Pp 109-

    2023  Volume 115

    Abstract: Objective: To study the histomorphological changes in cerebellar Purkinje cells after exposure to fine particulate matter in C57BL/6J mice. Study Design: Laboratory based experimental study. Place and Duration of Study: The study was conducted in the ... ...

    Abstract Objective: To study the histomorphological changes in cerebellar Purkinje cells after exposure to fine particulate matter in C57BL/6J mice. Study Design: Laboratory based experimental study. Place and Duration of Study: The study was conducted in the Anatomy department of the Army Medical College, Rawalpindi, from 15 June to 15 September 2020, in coordination with the Military Hospital, Rawalpindi, and the National Institute of Health (NIH), Islamabad. Materials and Methods: Thirty male and female C57BL/6 mice, 8 weeks of age, weighing 37± 2 gm were obtained from NIH, Islamabad. The animals were divided in two groups, 15 mice in each group (8 male and 7 female) Group A were marked as control, received regular diet and water ad libitum. Group B (experimental group) received dynamic inhalation of 3mg/m³ fine particles (soot) through air circulation for 6h/d for 12 weeks, in plastic cabin measuring 2x2x2 feet fitted with two small fans for evenly distribution of Particulate Matter. After exposure period, the animals were sacrificed. After sectioning the tissue and staining, the microscopic analysis was carried out. Purkinje cell margins were evaluated. Number of Purkinje cells and changes in Purkinje cell size were noted. Data was collected, analyzed with the statistical package for social sciences version 23. A p value ≤ 0.05 was considered significant. Result: The Purkinje cell margins were observed to become irregular and corrugated in the experimental groups B when compared with control group A. The number and size of Purkinje cells also showed difference when compared to the control group A. Conclusion: The present study concluded that fine particulate matter induces changes in histomorphological features of mice cerebellar tissue including Purkinje cells.
    Keywords air pollution ; cerebellum ; fine particulate matter ; purkinje cells ; Medicine ; R
    Subject code 630
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher Riphah International University, Islamabad
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: EFFECT OF PRUNUS DULCIS ON EPITHELIAL DAMAGE CAUSED BY NONSTEROIDAL ANTI-IFLAMMATORY DRUGS

    Muhammad Saad Abdullah / Shabnam Hamid / Shadab Ahmed Butt

    Pakistan Armed Forces Medical Journal, Vol 70, Iss 2, Pp 333-

    2020  Volume 337

    Abstract: Objective: To assess the effects of Prunus dulcis on gastric epithelium affected by nonsteroidal anti-inflammatory drugs. Study Design: Laboratory based experimental study. Place and Duration of Study: Anatomy Department, Army Medical College Rawalpindi, ...

    Abstract Objective: To assess the effects of Prunus dulcis on gastric epithelium affected by nonsteroidal anti-inflammatory drugs. Study Design: Laboratory based experimental study. Place and Duration of Study: Anatomy Department, Army Medical College Rawalpindi, in collaboration with National Institute of Health (NIH), Islamabad, from Jan 2015 to Dec 2015. Methodology: Forty healthy adult male and female (BALB/c strain) mice, 9-11 weeks age, weighting 30-40gm were used in the experiment and were kept in standard conditions in animal house. Animals were divided into four equal groups. Group A served as control, whereas group B, C and D served as experimental groups. Animals in all groups were given standard lab diet for 60 days. In addition to standard lab diet, experimental groups C and D were given almond oil (extracted without peel of almonds) and finely ground almonds (with peel), respectively, via oral gavage once a day for 60 days. At 60th day, 400 mg/kg of ASA solution was given, via oral gavage needle, to induce gastric ulcers in animals of experimental groups B, C and D. Type of epithelium and height of epithelium were assessed under Light Microscope. Results: Height and type of epithelium improved in experimental groups C and D on intergroup comparison. Whereas group D showed improved protection as compared to group C. Conclusion: Prunus dulcis provides protection, to gastric epithelium, against gastric ulcers induced by non-steroidal anti inflammatory drugs. In addition, finely granular almonds provided better protective outcome in comparison with almond oil.
    Keywords acetylsalicylic acid ; nonsteroidal anti-inflammatory drugs ; Medicine ; R ; Medicine (General) ; R5-920
    Subject code 630
    Language English
    Publishing date 2020-04-01T00:00:00Z
    Publisher Army Medical College Rawalpindi
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: EFFECT OF FINE PARTICULATE MATTER EXPOSURE ON TRIGGERING DEPRESSIVE-LIKE RESPONSE AND TOTAL BODY WEIGHT IN C57BL/6J MICE

    Saima Saleem / Shabnam Hamid / Maria Iram / Abdul Basit Jilani / Faiza Umbreen / Iram Zakria

    Pakistan Armed Forces Medical Journal, Vol 71, Iss 6, Pp 2114-

    2021  Volume 2117

    Abstract: Objective: To study the effect of fine particulate matter exposure in instigating depressive-like response and total body weight changes in C57BL/6J mice. Study Design: Laboratory-based experimental study. Place and Duration of Study: Anatomy Department, ...

    Abstract Objective: To study the effect of fine particulate matter exposure in instigating depressive-like response and total body weight changes in C57BL/6J mice. Study Design: Laboratory-based experimental study. Place and Duration of Study: Anatomy Department, Army Medical College, Rawalpindi, in collaboration with the Military Hospital, Rawalpindi and the National Institute of Health (NIH), Islamabad, from Jun to Sep 2020. Methodology: Thirty male and female C57BL/6 mice, 8 weeks of age, weighing 37 ± 2 gm were obtained from NIH, Islamabad. They were retained in separate cages in the animal house of NIH under standard laboratory conditions. The animals were divided into two groups, 15 mice in each group (8 males and 7 females) Group A was marked as control, received regular diet and water ad libitum. Group B (experimental group) received dynamic inhalation of 3 mg/m³ fine particles (soot) through air circulation for 6h/d for 12 weeks, in a plastic cabin measuring 2x2x2 feet fitted with two small fans for evenly distribution of PM. The initial and final weight of animals was recorded, and a tail suspension test was performed to assess depression in form of immobility. Result: The experimental group after exposure to fine particulate matter showed decreased activity and behavioural deficits with p-value <0.001 and loss in total body weight as compared to the control group with a p-value <0.001. Conclusion: Fine particulate matter has a significant effect on behaviour and cognition. It also causes a decrease in the total body weight of animals due to stress and agitation.
    Keywords fine particulate matter (fpm) ; tail suspension test (tst) ; depressive behavior ; stress ; Medicine ; R ; Medicine (General) ; R5-920
    Subject code 630
    Language English
    Publishing date 2021-12-01T00:00:00Z
    Publisher Army Medical College Rawalpindi
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: EFFECT OF NIGELLA SATIVA ON SERUM TESTOSTERONE LEVELS IN LETROZOLE INDUCED POLYCYSTIC OVARIAN SYNDROME IN MICE

    Noreen Anwar / Shabnam Hamid / Amer Nadeem / Ayesha Asad / Naureen Waseem / Nomana Mehmood

    Pakistan Armed Forces Medical Journal, Vol 71, Iss 1, Pp 338-

    2021  Volume 342

    Abstract: Objective: Monitor the ameliorative impact of Nigella sativa on fasting blood glucose and serum testosterone levels in polycystic ovarian syndrome caused by letrozole in mice. Study Design: Laboratory based randomized control trial. Place and Duration of ...

    Abstract Objective: Monitor the ameliorative impact of Nigella sativa on fasting blood glucose and serum testosterone levels in polycystic ovarian syndrome caused by letrozole in mice. Study Design: Laboratory based randomized control trial. Place and Duration of Study: Anatomy department, Army Medical College in teamwork with National Institute of Health. Time span is Dec 2014 Dec 2015. Methodology: Total 40 mature female BALB/c mice were chosen and distributed in four groups (ten mice in each one). Group 1 was given normal diet. Rests of three groups were treated with Letrozole at dose of 1 mg/kg body weight once daily for eight weeks. Group 3 was also given Nigella sativa seeds powder at dose of 10 g/kg body weight once daily starting at 22 day and continued up to eight weeks. Group 4 was treated with Nigella sativa oil at a dose of 4 ml/kg body weight once daily starting at 22 day and continued up to eight weeks. At termination of trial, fasting blood glucose level (FBG) was recorded by taking blood sample by glucometer from mouse lateral tail vein after 12 hours fasting. Mice with blood glucose level 250-464 mg/dl were marked hyperglycemic. Final blood sample was taken through cardiac puncture for serum testosterone evaluation. Results: Fasting blood glucose and Serum testosterone levels were raised in group 2 animals in comparison to group 1 while they were reduced remarkably in group 3 and 4 in comparison of group 2. Conclusion: Nigella sativa seeds in both solid and liquid state leave an identical curative impact on fasting blood glucose and serum testosterone levels in polycystic ovarian syndrome in mice.
    Keywords fasting blood glucose ; letrozole ; nigella sativa ; polycystic ovarian syndrome ; serum testosterone level ; Medicine ; R ; Medicine (General) ; R5-920
    Language English
    Publishing date 2021-02-01T00:00:00Z
    Publisher Army Medical College Rawalpindi
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: INFLAMMATION OF INTERSTITIAL CONNECTIVE TISSUE SPACE INDUCED BY LEAD ACETATE AND AMELIORATIVE AFTERMATH OF FICUSCARICAON RAT TESTIS

    Ayesha Asad / Shabnam Hamid / Afnan Gul / Noreen Anwar / Dujanah Bhatti / Naureen Waseem

    Pakistan Armed Forces Medical Journal, Vol 71, Iss 3, Pp 1066-

    2021  Volume 70

    Abstract: Objective: To observe inflammation of interstitial connective tissue space caused by Lead acetate in rat testis and ameliorative after math caused by Ficuscarica. Study Design: Laboratory based experimental study. Place and Duration of Study: Anatomy ... ...

    Abstract Objective: To observe inflammation of interstitial connective tissue space caused by Lead acetate in rat testis and ameliorative after math caused by Ficuscarica. Study Design: Laboratory based experimental study. Place and Duration of Study: Anatomy Department, Army Medical College Rawalpindi together with NIH (National Institute of Health) Islamabad, from Mar to Nov 2017. Methodology: Sprague Dawley male rats, 30 in quantity were chosen and 10 animals each partited into 3 groups. Treatments were given for 8 weeks, once daily. Group A was control group. Group B was treated with dosage of 30 mg/kg of Lead acetate. Group C was given dosage of 30 mg/kg of Lead acetate as well as 80 mg/kg of Ficuscarica. Twenty four hours after the concluding dose, animals were vivisected. For histological study, testis were fixed and stained with Haematoxylin and eosin. Interstitial connective tissue space thickness was morphometrically and assessed by SPSS version 22. A p-value <0.05 was considered significant in results. Results: Interstitial space thickness was significantly increased due to inflammation (>3 times normal) in group B in comparison to groups A and C. Thickness of space was slightly increased (<2 times normal) in group C in comparison to groups A due to reduction in inflammation. Conclusion: There was increased thickness of interstitium due to inflammation, cellular congestion and lymphocytic infiltration in rat’s testis because of lead acetate but concomitant dose of Ficuscarica protects against inflammation, venous congestion of interstitial space.
    Keywords ficuscarica ; inflammation ; interstitial connective tissue space ; lead acetate ; Medicine ; R ; Medicine (General) ; R5-920
    Subject code 630
    Language English
    Publishing date 2021-06-01T00:00:00Z
    Publisher Army Medical College Rawalpindi
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: EFFECT OF NIGELLA SATIVA ON NUMBER OF CYSTIC FOLLICLES IN LETROZOLE INDUCED POLYCYSTIC OVARIES IN MICE

    Noreen Anwar / Shabnam Hamid / Shadab Ahmed Butt

    Pakistan Armed Forces Medical Journal, Vol 66, Iss 3, Pp 310-

    2016  Volume 313

    Abstract: Objective: To observe the protective effect of Nigella sativa on number of cystic follicles in Letrozole induced polycystic ovaries in mice. Study Design: Laboratory based randomized control trial. Place and Duration of Study: Department of Anatomy, Army ...

    Abstract Objective: To observe the protective effect of Nigella sativa on number of cystic follicles in Letrozole induced polycystic ovaries in mice. Study Design: Laboratory based randomized control trial. Place and Duration of Study: Department of Anatomy, Army Medical College in collaboration with National Institute of Health from Nov 2014 to Nov 2015. Material and Methods: Forty female BALB/c mice were selected and divided in four groups, each having 10 animals. Group A served as control and was given normal diet. Group B was given Letrozole at a dose of 1milligram/kilogram body weight. Group C was treated with Letrozole for eight weeks at a dose of 1milligram/kilogram body weight and Nigella sativa seeds powder at a dose of 10grams/kilogram body weight once daily starting at 22 day and continued up to eight weeks. Group D was treated with Letrozole for eight weeks at a dose of 1milligram/kilogram body weight and Nigella sativa oil at a dose of 4milliliter/kilogram body weight once daily starting at 22 day and continued up to eight weeks. Animals were dissected a day after last dose. Size, shape, color and consistency of ovary was observed. Right ovary was processed, embedded and stained for histological study. Number of cystic follicles were counted and noted. Results: Significant number of cystic follicles was observed in ovaries of animals of group B as compared to group A. While their number decreased significantly in group C and D as compared to group B. Conclusion: Nigella sativa seeds powder and its oil, both have a similar protective effect on histomorphology of ovary of polycystic ovarian syndrome (PCOS) in mice by decreasing the number of cystic follicles.
    Keywords : Cystic follicles ; Letrozole ; Nigella sativa ; Medicine ; R ; Medicine (General) ; R5-920
    Subject code 630
    Language English
    Publishing date 2016-06-01T00:00:00Z
    Publisher Army Medical College Rawalpindi
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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