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  1. Article ; Online: The potential of Lactobacillus buchneri isolated from spontaneous rabbit meat fermented - bekasam against Salmonella typhimurium by in vivo evaluation

    EKA WULANDARI / HUSMY YURMIATI / TOTO SUBROTO / OKTA WISMANDANU / SHAFIA KHAIRANI

    Biodiversitas, Vol 23, Iss

    2022  Volume 5

    Abstract: Abstract. Wulandari E, Yurmiati H, Subroto T, Wismandanu O, Khairani S. 2022. The potential of Lactobacillus buchneri isolated from spontaneous rabbit meat fermented - bekasam against Salmonella typhimurium by in vivo evaluation. Biodiversitas 23: 2304- ... ...

    Abstract Abstract. Wulandari E, Yurmiati H, Subroto T, Wismandanu O, Khairani S. 2022. The potential of Lactobacillus buchneri isolated from spontaneous rabbit meat fermented - bekasam against Salmonella typhimurium by in vivo evaluation. Biodiversitas 23: 2304-2310. Rabbit meat bekasam is a traditional fermented food and is considered a healthy and functional food due to its nutrient content and microorganism. Lactic acid bacteria (LAB) was dominant microorganism and Lactobacillus buchneri E3 is one of LAB isolated during fermentation of the bekasam. Lactobacillus buchneri E3 has antimicrobial activity against pathogen bacteria. The present study aimed to investigate in vivo assessment of antimicrobial activity against Salmonella typhimurium using BALB/c mice. Oral administrations of three doses of L. buchneri E3(108, 109, and 1010 CFU/day/mouse) were performed for seven consecutive days. On the 8th day, each animal was inoculated with a single S. typhimurium, and on the 13th day, the mice were sacrificed for observation. The result showed that oral administration of L. buchneri E3 significantly increased the total population of LAB, significantly decreased S. typhimurium populations in the intestines, liver, and spleen, while increasing beneficial bacterial population and maintaining the normal hematology in the mice. The probiotic also maintained the histological examination of spleen and liver. These suggest that L. buchneri E3 is safe and could be used as the starter for fermentation products.
    Keywords balb/c ; enteropathogenic ; in vivo ; lactobacillus buchneri e3 ; probiotic ; Biology (General) ; QH301-705.5
    Subject code 630
    Language English
    Publishing date 2022-04-01T00:00:00Z
    Publisher MBI & UNS Solo
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: A Comparative Histological Study of Skin in Clarias gariepinus and Oreochromis niloticus

    Shafia Khairani / Faisal Fikri / Agus Purnomo / Muhammad Thohawi Elziyad Purnama

    Media Kedokteran Hewan, Vol 32, Iss 2, Pp 90-

    2021  Volume 96

    Abstract: This study aimed to evaluate the comparative of histological figures in Clarias gariepinus and Oreochromis niloticus skin. A total of 18 samples were observed and collected the skin preservation. The hematoxylin-eosin staining performed to determine the ... ...

    Abstract This study aimed to evaluate the comparative of histological figures in Clarias gariepinus and Oreochromis niloticus skin. A total of 18 samples were observed and collected the skin preservation. The hematoxylin-eosin staining performed to determine the histological structure. Results showed that there were different structures in the epidermis and dermis layer between C. gariepinus and O. niloticus skin. Club cells showed dominated in the epidermis layer of C. gariepinus. The pigmented cell showed clearly in the basement layer of C. gariepinus compared to O. niloticus. Stratum compactum in O. niloticus also showed adequate compared to C. gariepinus.
    Keywords clarias gariepinus ; oreochromis niloticus ; skin ; histology ; Veterinary medicine ; SF600-1100
    Language English
    Publishing date 2021-05-01T00:00:00Z
    Publisher Universitas Airlangga
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Oral Administration of Piperine as Curative and Prophylaxis Reduces Parasitaemia in Plasmodium berghei ANKA-Infected Mice

    Shafia Khairani / Nisa Fauziah / Hesti Lina Wiraswati / Ramdan Panigoro / Endang Yuni Setyowati / Afiat Berbudi

    Journal of Tropical Medicine, Vol

    2022  Volume 2022

    Abstract: Malaria remains a public health problem and a leading cause of death worldwide. Consequently, the discovery of novel agents, including substances from medicinal plants, is urgently needed. Piper nigrum has long been used by the community in the treatment ...

    Abstract Malaria remains a public health problem and a leading cause of death worldwide. Consequently, the discovery of novel agents, including substances from medicinal plants, is urgently needed. Piper nigrum has long been used by the community in the treatment of the symptoms of malaria. In a previous study, Piper nigrum was demonstrated to exhibit promising antiplasmodial activity against Plasmodium falciparum 3D7 and INDO strains. The aim of this study was to further investigate the antimalarial activity (curative and prophylactic) of piperine (a major isolated constituent of Piper nigrum) in Plasmodium berghei ANKA-infected mice. Piperine 10, 20, and 40 mg/kg body weight (bw), artesunate 5 mg/kg bw, and DMSO were administered orally for four days to different groups of Swiss Webster mice. Then, mice were monitored for parasitaemia, body weight, rectal temperature, survival rate, and clinical parameters. Piperine 40 mg/kg bw in curative and prophylactic tests had the maximum parasitaemia chemosuppression of 79.21% and 58.8% (p < 0.05), respectively, with a significant effect on the survival rate compared with control animals. In the curative test, piperine 40 mg/kg bw reduced the mean clinical score compared with the control group. Additionally, piperine showed an ability to protect organs (lungs, liver, spleen, and kidneys) from some damage in a dose-dependent manner. This study can be used as a basis for further discovery of novel chemotherapeutic or chemoprophylactic compounds.
    Keywords Arctic medicine. Tropical medicine ; RC955-962
    Subject code 630
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article: The potential of Lactobacillus buchneri isolated from spontaneous rabbit meat fermented - bekasam against Salmonella typhimurium by in vivo evaluation

    EKA WULANDARI / HUSMY YURMIATI / TOTO SUBROTO / OKTA WISMANDANU / SHAFIA KHAIRANI

    Biodiversitas. 2022 Apr., v. 23, no. 5

    2022  

    Abstract: Wulandari E, Yurmiati H, Subroto T, Wismandanu O, Khairani S. 2022. The potential of Lactobacillus buchneri isolated from spontaneous rabbit meat fermented - bekasam against Salmonella typhimurium by in vivo evaluation. Biodiversitas 23: 2304-2310. ... ...

    Abstract Wulandari E, Yurmiati H, Subroto T, Wismandanu O, Khairani S. 2022. The potential of Lactobacillus buchneri isolated from spontaneous rabbit meat fermented - bekasam against Salmonella typhimurium by in vivo evaluation. Biodiversitas 23: 2304-2310. Rabbit meat bekasam is a traditional fermented food and is considered a healthy and functional food due to its nutrient content and microorganism. Lactic acid bacteria (LAB) was dominant microorganism and Lactobacillus buchneri E3 is one of LAB isolated during fermentation of the bekasam. Lactobacillus buchneri E3 has antimicrobial activity against pathogen bacteria. The present study aimed to investigate in vivo assessment of antimicrobial activity against Salmonella typhimurium using BALB/c mice. Oral administrations of three doses of L. buchneri E3(108, 109, and 1010 CFU/day/mouse) were performed for seven consecutive days. On the 8th day, each animal was inoculated with a single S. typhimurium, and on the 13th day, the mice were sacrificed for observation. The result showed that oral administration of L. buchneri E3 significantly increased the total population of LAB, significantly decreased S. typhimurium populations in the intestines, liver, and spleen, while increasing beneficial bacterial population and maintaining the normal hematology in the mice. The probiotic also maintained the histological examination of spleen and liver. These suggest that L. buchneri E3 is safe and could be used as the starter for fermentation products.
    Keywords Lactobacillus buchneri ; Salmonella Typhimurium ; antimicrobial properties ; fermentation ; fermented foods ; functional foods ; hematology ; histology ; lactic acid ; liver ; mice ; nutrient content ; oral administration ; pathogens ; probiotics ; rabbit meat ; spleen
    Language English
    Dates of publication 2022-04
    Publishing place MBI & UNS Solo
    Document type Article
    ZDB-ID 2660049-3
    ISSN 2085-4722
    ISSN 2085-4722
    DOI 10.13057/biodiv/d230508
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: The Potential use of a Curcumin-Piperine Combination as an Antimalarial Agent

    Shafia Khairani / Nisa Fauziah / Hesti Lina Wiraswati / Ramdan Panigoro / Endang Yuni Setyowati / Afiat Berbudi

    Journal of Tropical Medicine, Vol

    A Systematic Review

    2021  Volume 2021

    Abstract: Malaria remains a significant global health problem, but the development of effective antimalarial drugs is challenging due to the parasite’s complex life cycle and lack of knowledge about the critical specific stages. Medicinal plants have been ... ...

    Abstract Malaria remains a significant global health problem, but the development of effective antimalarial drugs is challenging due to the parasite’s complex life cycle and lack of knowledge about the critical specific stages. Medicinal plants have been investigated as adjuvant therapy for malaria, so this systematic review summarizes 46 primary articles published until December 2020 that discuss curcumin and piperine as antimalarial agents. The selected articles discussed their antioxidant, anti-inflammatory, and antiapoptosis properties, as well as their mechanism of action against Plasmodium species. Curcumin is a potent antioxidant, damages parasite DNA, and may promote an immune response against Plasmodium by increasing reactive oxygen species (ROS), while piperine is also a potent antioxidant that potentiates the effects of curcumin. Hence, combining these compounds is likely to have the same effect as chloroquine, that is, attenuate and restrict parasite development, thereby reducing parasitemia and increasing host survival. This systematic review presents new information regarding the development of a curcumin-piperine combination for future malaria therapy.
    Keywords Arctic medicine. Tropical medicine ; RC955-962
    Subject code 572
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Effects of dietary supplementation with breadfruit leaf powder on growth performance, meat quality, and antioxidative activity in Japanese quail

    Muhammad Thohawi Elziyad Purnama / Eric Putra Ernanda / Faisal Fikri / Agus Purnomo / Shafia Khairani / Shekhar Chhetri

    Veterinary World, Vol 14, Iss 7, Pp 1946-

    2021  Volume 1953

    Abstract: Background and Aim: In an era of increasing concerns about food availability globally, poultry meat is being increasingly consumed rather than red meat given its quality in terms of pH, color, and tenderness, conferring consumer satisfaction. The choice ... ...

    Abstract Background and Aim: In an era of increasing concerns about food availability globally, poultry meat is being increasingly consumed rather than red meat given its quality in terms of pH, color, and tenderness, conferring consumer satisfaction. The choice of feed is a crucial factor in poultry production. This study investigated the effect of dietary supplementation with breadfruit leaf powder on growth performance, meat quality, and antioxidative activity in Japanese quail. Materials and Methods: A total of 120 day-old quail were used in this study and assigned equally into four treatment groups: Group C fed a basal diet and three treatment groups fed a basal diet supplemented with 2.5% (T1), 5% (T2), or 10% (T3) breadfruit leaf powder. The concentrations of breadfruit leaf powder were 2.5, 5, and 10 g/kg in the basal diet. Quail body weight and feed intake (FI) were evaluated at 1, 21, and 35 days of age at 7 a.m. Pectoral muscle was collected to determine pH, meat color, drip loss, cooking loss, water-holding capacity (WHC), tenderness, and antioxidant levels. All variables were analyzed statistically using ANOVA followed by Duncan's post hoc test (significance set at p<0.05). Results: T3 showed increased body weight gain of quails at 1-21 and 21-35 days (p<0.05). Feeding in the T3 group improved the feed conversion ratio compared with those in the C and T1 groups at the starter phase (p<0.05). Dietary treatment did not affect FI (p>0.05). In the present study, meat redness and WHC were improved in the T3 group (p<0.05). Meanwhile, drip loss, cooking loss, and meat tenderness were improved in the T2 group (p<0.05). The pH45 min, pH24 h, lightness, and yellowness were not influenced by the treatments (p>0.05). The antioxidative activities of superoxide dismutase and malondialdehyde decreased in the T3 group (p<0.05), while no significant difference in glutathione peroxidase level (p>0.05) was identified. Conclusion: Ten grams/kilogram of breadfruit leaf powder, as administered in the ...
    Keywords antioxidative activity ; breadfruit leaf powder ; food availability ; growth performance ; japanese quail ; meat quality ; Animal culture ; SF1-1100 ; Veterinary medicine ; SF600-1100
    Subject code 630
    Language English
    Publishing date 2021-07-01T00:00:00Z
    Publisher Veterinary World
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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