Article ; Online: Influence of cytochrome P450 and glutathione S transferase polymorphisms on response to nilotinib therapy among chronic myeloidleukemia patients from Pakistan.
2022 Volume 22, Issue 1, Page(s) 519
Abstract: Background: Cytochrome P450 (CYP) and glutathione S transferases (GSTs) are important biotransforming enzymes responsible for detoxification of anticancer drugs and carcinogens. Polymorphisms in these enzymes may greatly influence the susceptibility to ... ...
Abstract | Background: Cytochrome P450 (CYP) and glutathione S transferases (GSTs) are important biotransforming enzymes responsible for detoxification of anticancer drugs and carcinogens. Polymorphisms in these enzymes may greatly influence the susceptibility to CML and overall efficacy of tyrosine kinase inhibitors. This study was aimed to estimate the possible influence of the polymorphisms of GSTs and CYP in the occurrence of CML as well as in predicting therapeutic outcome of nilotinib therapy in Pakistani CML patients. Methods: The polymorphic variability in CYP 1A1*2C, GSTP1 (A3131G), GSTT1 and GSTM1 was assessed either by RFLP or multiplex PCR. The BCR ABL1 transcripts were quantified by qPCR to monitor response to nilotinib. Results: The CYP1A1*2C heterozygous and GSTP1 homozygous polymorphisms seemed to be a contributing factor in developing CML. Altogether, there were 12 non-responders, 66 responders and 21 partial responders. The most frequent genotype was null GSTM1 in responders followed by CYP 1A1 and GSTP1 -wild type (p = < 0.05). Whereas, homozygous GSTP1 and GSTT1 null genotype is significantly higher only among nilotinib non-responders. Conclusion: Hence, it can be concluded that wild type CYP1A1, GSTP1 and null GSTM1 may be frequently linked to favorable outcome in patients treated with nilotinib as depicted by sustained deep molecular response in most CML patients. |
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MeSH term(s) | Case-Control Studies ; Cytochrome P-450 CYP1A1/genetics ; Genetic Predisposition to Disease ; Genotype ; Glutathione S-Transferase pi/genetics ; Glutathione Transferase/genetics ; Humans ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/drug therapy ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics ; Pakistan ; Polymorphism, Genetic ; Pyrimidines/therapeutic use ; Risk Factors |
Chemical Substances | Pyrimidines ; Cytochrome P-450 CYP1A1 (EC 1.14.14.1) ; glutathione S-transferase T1 (EC 2.5.1.-) ; GSTP1 protein, human (EC 2.5.1.18) ; Glutathione S-Transferase pi (EC 2.5.1.18) ; Glutathione Transferase (EC 2.5.1.18) ; glutathione S-transferase M1 (EC 2.5.1.18) ; nilotinib (F41401512X) |
Language | English |
Publishing date | 2022-05-08 |
Publishing country | England |
Document type | Journal Article |
ZDB-ID | 2041352-X |
ISSN | 1471-2407 ; 1471-2407 |
ISSN (online) | 1471-2407 |
ISSN | 1471-2407 |
DOI | 10.1186/s12885-022-09605-1 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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