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  1. Book ; Online ; E-Book: Biochips and medical imaging

    Wang, Shan Xiang / De la Zerda, Adam

    2022  

    Abstract: Biochips are essentially miniaturized laboratories that can perform hundreds or thousands of simultaneous biochemical reactions. Biochips enable researchers to quickly screen large numbers of biological analytes for a variety of purposes, from disease ... ...

    Author's details Adam De la Zerda, Shan X. Wang
    Abstract "Biochips are essentially miniaturized laboratories that can perform hundreds or thousands of simultaneous biochemical reactions. Biochips enable researchers to quickly screen large numbers of biological analytes for a variety of purposes, from disease diagnosis to detection of bioterrorism agents. Protein microarrays, antibody microarrays, and chemical compound microarrays can also be produced using biochips"--
    Keywords Biochips ; Diagnostic imaging
    Subject code 610.28
    Language English
    Size 1 online resource (429 pages)
    Publisher John Wiley & Sons Inc
    Publishing place Hoboken, New Jersey
    Document type Book ; Online ; E-Book
    Remark Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
    ISBN 1-118-91063-X ; 1-118-91048-6 ; 1-118-91057-5 ; 1-118-91050-8 ; 978-1-118-91063-4 ; 978-1-118-91048-1 ; 978-1-118-91057-3 ; 978-1-118-91050-4
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Article: Effects of the novel sodium-dependent phosphate cotransporter 2b inhibitor DZ1462 on hyperphosphatemia in chronic kidney disease.

    Lu, Xiao / Yu, Ling-Zhi / Guo, Qiu-Li / Zhang, Lin / Jiang, Shan-Xiang

    American journal of translational research

    2024  Volume 16, Issue 3, Page(s) 768–780

    Abstract: Background: Serum phosphate levels remain insufficiently controlled in chronic kidney disease (CKD) patients, and novel therapeutic strategies are needed. Blocking intestinal phosphate absorption mediated by sodium-dependent phosphate cotransporter type ...

    Abstract Background: Serum phosphate levels remain insufficiently controlled in chronic kidney disease (CKD) patients, and novel therapeutic strategies are needed. Blocking intestinal phosphate absorption mediated by sodium-dependent phosphate cotransporter type 2b (NPT2b) holds promise as one such strategy.
    Methods: The in vitro cellular potency of DZ1462 was evaluated using a radioactive Pi uptake assay on stable Chinese hamster ovary (CHO) cell clones transfected with human NPT2b (hNPT2b) or rat NPT2b (rNPT2b). The ability of DZ1462 to inhibit phosphate absorption was studied in vivo in an acute model after oral bolus challenge with
    Results: The cellular assays with the hNPT2b-CHO and rNPT2b-CHO clones showed that DZ1462 significantly and potently inhibited phosphate uptake. In vivo, in a chronic Pi-fed rat model, DZ1462 effectively inhibited intestinal Pi uptake. In a hyperphosphatemia rat model, DZ1462 significantly inhibited Pi uptake, and DZ1462 in combination with sevelamer had a synergistic effect. The pharmacokinetics (PK) study confirmed that DZ1462 is a gastrointestinal (GI)-restricted compound that can remain in the intestine for a sufficient duration. In addition, DZ1462 also reduced cardiovascular events and ameliorated osteoporosis in a CKD animal model.
    Conclusions: This study revealed that a GI-restricted NPT2b inhibitor DZ1462 potently inhibits NPT2b in vitro and blocks intestinal phosphate uptake in multiple animal models with potential to reduce various cardiovascular events in CKD models. Therefore, DZ1462 may be useful to treat renal disease patients who have shown an unsatisfactory response to phosphate binders.
    Language English
    Publishing date 2024-03-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2471058-1
    ISSN 1943-8141
    ISSN 1943-8141
    DOI 10.62347/UGTW5471
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  3. Article ; Online: Enhanced complex local frequency elastography method for tumor viscoelastic shear modulus reconstruction.

    Hu, Liangliang / Shan, Xiang

    Computer methods and programs in biomedicine

    2020  Volume 195, Page(s) 105605

    Abstract: Background and objectives: The Mayo Clinic provides a magnetic resonance (MR) elastography software named MRE Wave, which uses the conventional local frequency elastography (LFE) method. However, MRE Wave is unable to supply complex viscoelasticity maps ...

    Abstract Background and objectives: The Mayo Clinic provides a magnetic resonance (MR) elastography software named MRE Wave, which uses the conventional local frequency elastography (LFE) method. However, MRE Wave is unable to supply complex viscoelasticity maps for elastography. We sought to improve the local frequency estimation algorithm used in LFE, which we refer to as the Enhanced Complex Local Frequency Elastography (EC-LFE) algorithm.
    Methods: The proposed algorithm uses wave equations under the hypotheses of being linear, isotropic, and locally homogeneous. Two 2D simulation models were used to investigate the accuracy and sensitivity of the EC-LFE algorithm for detecting small tumors. The corresponding statistical parameters were the relative root mean square (RMS) error and contrast-to-noise ratio (CNR). EC-LFE was investigated with two different parameter sets, one with an optimally chosen parameter ξ (EC-LFE Adj, for short) and the other with ξ = 0 (EC-LFE0). We compared the MRE Wave and the EC-LFE using series signal-to-noise (SNR) wave data.
    Results: The elasticity RMS error of the MRE Wave software was about 1%, and that of the EC-LFE0 and EC-LFE Adj were about 0.2%. The elasticity standard deviation of the MRE Wave software was about 3% of the mean value, and those of the EC-LFE0 and EC-LFE Adj were about 1% of the mean value. The elasticity CNR value of EC-LFE0 reached 1.93 times that of the MRE Wave in the region of small tumors (less than 10-point sampling). The viscosity RMS errors of the EC-LFE0 could be less than 5%.
    Conclusion: Compared to conventional methods, the EC-LFE was more accurate and sensitive for small tumor detection and exhibited higher noise immunity. The improved algorithm output more parameters and outperformed than the MRE Wave, thereby rendering them more suitable for clinical applications.
    MeSH term(s) Algorithms ; Elasticity ; Elasticity Imaging Techniques ; Humans ; Magnetic Resonance Imaging ; Neoplasms/diagnostic imaging ; Viscosity
    Language English
    Publishing date 2020-06-12
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 632564-6
    ISSN 1872-7565 ; 0169-2607
    ISSN (online) 1872-7565
    ISSN 0169-2607
    DOI 10.1016/j.cmpb.2020.105605
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  4. Article: Resuscitative endovascular balloon occlusion of the aorta in the treatment of severe hemorrhagic shock caused by upper gastrointestinal bleeding.

    Li, Yu-Lin / Wang, Jun-Yang / Han, Wen / Sun, Tao / Lyu, Kang / Xu, Shan-Xiang

    World journal of emergency medicine

    2023  Volume 14, Issue 3, Page(s) 247–249

    Language English
    Publishing date 2023-05-02
    Publishing country China
    Document type Journal Article
    ZDB-ID 2753264-1
    ISSN 1920-8642
    ISSN 1920-8642
    DOI 10.5847/wjem.j.1920-8642.2023.051
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  5. Article ; Online: Deep neural networks for magnetic resonance elastography acceleration in thermal-ablation monitoring.

    Shan, Xiang / Yang, Jinying / Xu, Peng / Hu, Liangliang / Ge, Haitao

    Medical physics

    2022  Volume 49, Issue 3, Page(s) 1803–1813

    Abstract: Purpose: To develop a deep neural network for accelerating magnetic resonance elastography (MRE) acquisition, to validate the ability to generate reliable MRE results with the down-sampled k-space data, and to demonstrate the feasibility of the proposed ...

    Abstract Purpose: To develop a deep neural network for accelerating magnetic resonance elastography (MRE) acquisition, to validate the ability to generate reliable MRE results with the down-sampled k-space data, and to demonstrate the feasibility of the proposed method in monitoring the stiffness changes during thermal ablation in a phantom study.
    Materials and methods: MRE scans were performed with 60 Hz excitation on porcine ex-vivo liver gel phantoms in a 0.36T MRI scanner to generate the training dataset. The acquisition protocol was based on a spin-echo MRE pulse sequence with tailored motion-sensitive gradients to reduce echo time (TE). A U-Net based deep neural network was developed and trained to interpolate the missing data from down-sampled k-space. We calculated the errors of 80 sets magnitude/phase images reconstructed from the zero-filled, compressive sensing (CS) and deep learning (DL) method for comparison. The peak signal-to-noise rate (PSNR) and structural similarity index (SSIM) of the magnitude/phase images were also calculated for comparison. The stiffness changes were recorded before, during, and after ablation. The mean stiffness values over the region of interest (ROI) were compared between the elastograms reconstructed from the fully sampled k-space and interpolated k-space after thermal ablation.
    Results: The mean absolute error (MAE), PSNR, and SSIM of the proposed DL approach were significantly better than the results from the zero-filled method (p < 0.0001) and CS (p < 0.0001). The stiffness changes before and after thermal ablation assessed by the proposed approach (before: 7.7±1.1 kPa, after: 11.9±4.0 kPa, 4.2-kPa increase) gave close agreement with the values calculated from the fully sampled data (before: 8.0±1.0 kPa, after: 12.6±4.2 kPa, 4.6-kPa increase). In contrast, the stiffness changes computed from the zero-filled method (before: 4.9±1.4 kPa, after: 5.6±2.8 kPa, 0.7-kPa increase) were substantially underestimated.
    Conclusion: This study demonstrated the capability of the proposed DL method for rapid MRE acquisition and provided a promising solution for monitoring the MRI-guided thermal ablation.
    MeSH term(s) Acceleration ; Animals ; Echo-Planar Imaging/methods ; Elasticity Imaging Techniques/methods ; Magnetic Resonance Imaging/methods ; Neural Networks, Computer ; Swine
    Language English
    Publishing date 2022-02-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 188780-4
    ISSN 2473-4209 ; 0094-2405
    ISSN (online) 2473-4209
    ISSN 0094-2405
    DOI 10.1002/mp.15471
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  6. Article ; Online: Association Between Anatomic and Clinical Indicators of Injury Severity After Moderate-Severe Traumatic Brain Injury: A Pilot Study Using Multiparametric Magnetic Resonance Imaging.

    Esterov, Dmitry / Yin, Ziying / Persaud, Trevor / Shan, Xiang / Murphy, Mathew C / Ehman, Richard L / Huston, John / Brown, Allen W

    Neurotrauma reports

    2024  Volume 5, Issue 1, Page(s) 232–242

    Abstract: This study sought to identify whether an anatomical indicator of injury severity as measured by multiparametric magnetic resonance imaging (MRI) including magnetic resonance elastography (MRE), is predictive of a clinical measure of injury severity after ...

    Abstract This study sought to identify whether an anatomical indicator of injury severity as measured by multiparametric magnetic resonance imaging (MRI) including magnetic resonance elastography (MRE), is predictive of a clinical measure of injury severity after moderate-severe traumatic brain injury (TBI). Nine individuals who were admitted to acute inpatient rehabilitation after moderate-to-severe TBI completed a comprehensive MRI protocol prior to discharge from rehabilitation, which included conventional MRI with diffusion tensor imaging (DTI). Of those, five of nine also underwent brain MRE to measure the brain parenchyma stiffness. Clinical severity of injury was measured by the length of post-traumatic amnesia (PTA). MRI-assessed non-hemorrhage contusion score and hemorrhage score, DTI-measured white matter fractional anisotropy, and MRE-measured lesion stiffness were all assessed. A higher hemorrhagic score was significantly associated with a longer length of PTA (
    Language English
    Publishing date 2024-03-13
    Publishing country United States
    Document type Journal Article
    ISSN 2689-288X
    ISSN (online) 2689-288X
    DOI 10.1089/neur.2023.0122
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  7. Article ; Online: Diagenetic Genesis and Evolution of Coal-Bearing Tight Sandstone Reservoir in the Yangxia Formation, Northern Kuqa Depression, Tarim Basin.

    Guo, Huajun / Xia, Wenhao / Shan, Xiang / Xi, Kelai / Peng, Bo / Yang, Xianzhang / Zou, Zhiwen / Yuan, Wenfang

    ACS omega

    2024  Volume 9, Issue 16, Page(s) 18314–18326

    Abstract: Coal seams of the Yangxia Formation are widespread in the northern part of the Kuqa Depression in the Tarim Basin. During the thermal evolution of the coal seams, the generated fluids of different periods and natures have a significant impact on tight ... ...

    Abstract Coal seams of the Yangxia Formation are widespread in the northern part of the Kuqa Depression in the Tarim Basin. During the thermal evolution of the coal seams, the generated fluids of different periods and natures have a significant impact on tight sandstone reservoirs. To further investigate the diagenetic characteristics and reservoir genesis of the tight sandstones due to the influence of coal seams, an in-depth exploration of the causes of dissolution and cementation in the reservoirs was conducted through thin-section casting, cathode luminescence, scanning electron microscopy, carbon-oxygen isotopic analyses, and X-ray diffraction of whole rock and authigenic clay minerals, along with burial evolution history and fluid evolution history. It is suggested that two phases of acidic fluids are mainly produced during the thermal evolution process of coal seams, including an early humic acid and a late organic carboxylic acid. The early phase humic acid plays a purifying role in reservoirs with coarse particles, rigidity-rich particles, and good permeability conditions. It selectively dissolves sedimentary calcareous mud and calcite, and the dissolution products are completely migrated. At the same time, it inhibits early carbonate cementation. The late organic carboxylic acid will dissolve potassium feldspar and some volcanic rock debris, and the dissolution products are difficult to migrate under the sealing conditions caused by lithological differences, which often take the cementation form of siliceous overgrowth and kaolinite or illite. In addition to the cementation resulting from the dissolution products of acidic fluids produced by the coal seams, the CO
    Language English
    Publishing date 2024-04-11
    Publishing country United States
    Document type Journal Article
    ISSN 2470-1343
    ISSN (online) 2470-1343
    DOI 10.1021/acsomega.3c10100
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  8. Article ; Online: Using halofuginone-silver thermosensitive nanohydrogels with antibacterial and anti-inflammatory properties for healing wounds infected with Staphylococcus aureus.

    Zuo, Ru-Nan / Gong, Jia-Hao / Gao, Xiu-Ge / Huang, Jin-Hu / Zhang, Jun-Ren / Jiang, Shan-Xiang / Guo, Da-Wei

    Life sciences

    2024  Volume 339, Page(s) 122414

    Abstract: Contamination by pathogens, such as bacteria, can irritate a wound and prevent its healing, which may affect the physical fitness of the infected person. As such, the development of more novel nano-biomaterials able to cope with the inflammatory reaction ...

    Abstract Contamination by pathogens, such as bacteria, can irritate a wound and prevent its healing, which may affect the physical fitness of the infected person. As such, the development of more novel nano-biomaterials able to cope with the inflammatory reaction to bacterial infection during the wound healing process to accelerate wound healing is required. Herein, a halofuginone‑silver nano thermosensitive hydrogel (HTPM&AgNPs-gel) was prepared via a physical swelling method. HTPM&AgNPs-gel was characterized based on thermogravimetric analysis, differential scanning calorimetry, morphology, injectability, and rheological mechanics that reflected its exemplary nature. Moreover, HTPM&AgNPs-gel was further tested for its ability to facilitate healing of skin fibroblasts and exert antibacterial activity. Finally, HTPM&AgNPs-gel was tested for its capacity to accelerate general wound healing and treat bacterially induced wound damage. HTPM&AgNPs-gel appeared spherical under a transmission electron microscope and showed a grid structure under a scanning electron microscope. Additionally, HTPM&AgNPs-gel demonstrated excellent properties, including injectability, temperature-dependent swelling behavior, low loss at high temperatures, and appropriate rheological properties. Further, HTPM&AgNPs-gel was found to effectively promote healing of skin fibroblasts and inhibit the proliferation of Escherichia coli and Staphylococcus aureus. An evaluation of the wound healing efficacy demonstrated that HTPM&AgNPs-gel had a more pronounced ability to facilitate wound repair and antibacterial effects than HTPM-gel or AgNPs-gel alone, and exhibited ideal biocompatibility. Notably, HTPM&AgNPs-gel also inhibited inflammatory responses in the healing process. HTPM&AgNPs-gel exhibited antibacterial, anti-inflammatory, and scar repair features, which remarkably promoted wound healing. These findings indicated that HTPM&AgNPs-gel holds great clinical potential as a promising and valuable wound healing treatment.
    MeSH term(s) Humans ; Silver/pharmacology ; Silver/chemistry ; Staphylococcus aureus ; Wound Healing ; Metal Nanoparticles/chemistry ; Anti-Bacterial Agents/pharmacology ; Hydrogels/chemistry ; Anti-Inflammatory Agents/pharmacology ; Piperidines ; Quinazolinones
    Chemical Substances Silver (3M4G523W1G) ; halofuginone (L31MM1385E) ; Anti-Bacterial Agents ; Hydrogels ; Anti-Inflammatory Agents ; Piperidines ; Quinazolinones
    Language English
    Publishing date 2024-01-10
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 3378-9
    ISSN 1879-0631 ; 0024-3205
    ISSN (online) 1879-0631
    ISSN 0024-3205
    DOI 10.1016/j.lfs.2024.122414
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    Uc I Zs.368: Show issues Location:
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  9. Article ; Online: Metabolomic alternations of follicular fluid of obese women undergoing in-vitro fertilization treatment

    Jingyan Song / Shan Xiang / Conghui Pang / Jiayin Guo / Zhengao Sun

    Scientific Reports, Vol 10, Iss 1, Pp 1-

    2020  Volume 12

    Abstract: Abstract Obesity exerts negative effects on the metabolic homeostasis of cells in various tissues, but how it influences ovum metabolism is not fully understood. Previous studies demonstrate that oocyte genes that regulate oxidative stress, lipid ... ...

    Abstract Abstract Obesity exerts negative effects on the metabolic homeostasis of cells in various tissues, but how it influences ovum metabolism is not fully understood. Previous studies demonstrate that oocyte genes that regulate oxidative stress, lipid metabolism, and inflammation are highly expressed in obese women. However, the metabolic effects of these genetic variations are not clear. To address this gap, we conducted an exploratory evaluation of follicular fluid (FF) metabolites in underweight, normal-weight, overweight, and obese women undergoing in vitro fertilization (IVF) treatment. The FF samples from the underweight (Group A, n = 40), normal-weight (Group B, n = 40), overweight (Group C, n = 40), and obese women (Group D, n = 40) were analyzed using ultra-performance liquid chromatography high-resolution mass spectrometry. A novel, high-coverage, semi-targeted metabolomics method (SWATH to MRM) and a targeted metabolomics method were employed to identify and verify the differential metabolites between the four groups. Sixteen differentially expressed FF metabolites were identified. Increase of BMI was associated with upregulation of 5 metabolites, ganoderiol H, LPI (18:3), sedoheptulose 1,7-bisphosphate, austalide L and 2 - {[hydroxyl (3-hydroxy-4-methoxyphenylmethylidene] amino} acetic acid, and downregulation of 5 metabolites, 1-phenyl-1,3-elcosanedione, retinol acetate, p-Cresol sulfate, setariol and arachidonyl carnitine. These metabolites were enriched in different metabolic pathways of retinol metabolism and fatty acid metabolism. These obesity-related differential metabolites provide a pathogenesis mechanism that explains the decline of oocyte development during obesity. These results suggest that obesity affects follicular environment prior to pregnancy, a time-window that may be important for lifestyle interventions to decrease obesity levels.
    Keywords Medicine ; R ; Science ; Q
    Subject code 500
    Language English
    Publishing date 2020-04-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article: High-flow nasal cannula oxygen therapy and noninvasive ventilation for preventing extubation failure during weaning from mechanical ventilation assessed by lung ultrasound score: A single-center randomized study.

    Xu, Shan-Xiang / Wu, Chun-Shuang / Liu, Shao-Yun / Lu, Xiao

    World journal of emergency medicine

    2021  Volume 12, Issue 4, Page(s) 274–280

    Abstract: Background: We sought to demonstrate the superiority of a targeted therapy strategy involving high-flow nasal cannula oxygen (HFNCO: Methods: During the study period, 98 patients were enrolled in the study, including 49 in the control group and 49 in ...

    Abstract Background: We sought to demonstrate the superiority of a targeted therapy strategy involving high-flow nasal cannula oxygen (HFNCO
    Methods: During the study period, 98 patients were enrolled in the study, including 49 in the control group and 49 in the treatment group. Patients in the control group and patients with an LUS score <14 points (at low risk of extubation failure) in the treatment group were extubated and received standard preventive care without NIV or HFNCO
    Results: In the control group, 13 patients had the LUS scores ≥14 points, while 36 patients had scores <14 points. In the treatment group, 16 patients had the LUS scores ≥14 points, while 33 patients had scores <14 points. Among patients with the LUS score ≥14 points, the extubation failure rate within 48 hours was 30.8% in the control group and 12.5% in the treatment group, constituting a statistically significant difference (
    Conclusions: Among high-risk adults being weaned from mechanical ventilation and assessed by LUS, the NIV+HFNCO
    Language English
    Publishing date 2021-09-01
    Publishing country China
    Document type Journal Article
    ZDB-ID 2753264-1
    ISSN 1920-8642
    ISSN 1920-8642
    DOI 10.5847/wjem.j.1920-8642.2021.04.004
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